################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbeI BbsI BclI BfaI BfrI BglI BlnI BpuAI BsaBI BsaHI BsaI BseAI BsiWI BsmAI BsmBI BsmI BspDI BspEI BspHI BsrDI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI MaeI MamI MfeI MluI MroI MseI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccII 1562 CGCG CG^CG AflIII 847 ACATGT A^C[GA][CT]GT AlwI 1269 GATCC GGATC(4/5) Asp700I 621 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 110 TGGCCA TGG^CCA BbrPI 1612 CACGTG CAC^GTG BglII 1769 AGATCT A^GATCT BlpI 1282 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1282 GCTGAGC GC^T[ACGT]AGC BsaAI 1612 CACGTG [CT]AC^GT[GA] BsaWI 1231 TCCGGT [AT]^CCGG[AT] BsiEI 1539 CGACCG CG[GA][CT]^CG Bsp1407I 1206 TGTACA T^GTACA BspLU11I 847 ACATGT A^CATGT BspMI 1223 ACCTGC ACCTGC(4/8) BsrGI 1206 TGTACA T^GTACA BstUI 1562 CGCG CG^CG CelII 1282 GCTGAGC GC^T[ACGT]AGC EarI 1336 CTCTTC CTCTTC(1/4) EcoNI 1463 CCTTCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG Ksp632I 1336 CTCTTC CTCTTC(1/4) KspI 1561 CCGCGG CCGC^GG MluNI 110 TGGCCA TGG^CCA MscI 110 TGGCCA TGG^CCA MvnI 1562 CGCG CG^CG NaeI 1040 GCCGGC GCC^GGC NcoI 101 CCATGG C^CATGG NgoMIV 1040 GCCGGC G^CCGGC NspI 847 ACATGT [GA]CATG^[CT] PmaCI 1612 CACGTG CAC^GTG PmlI 1612 CACGTG CAC^GTG SacI 1333 GAGCTC GAGCT^C SacII 1561 CCGCGG CCGC^GG SapI 1335 GCTCTTC GCTCTTC(1/4) SspBI 1206 TGTACA T^GTACA XmnI 621 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### Alw44I 1109 GTGCAC G^TGCAC 1695 GTGCAC ApaLI 1109 GTGCAC G^TGCAC 1695 GTGCAC BanI 699 GGCACC G^G[CT][GA]CC 1743 GGTGCC BbvCI 236 CCTCAGC CCTCAGC(-5/-2) 272 CCTCAGC BcgI 946 CGAAACCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1494 CGATGTCCCTGC BsgI 1477 CTGCAC GTGCAG(16/14) 883 GTGCAG BsrBI 348 GAGCGG CCGCTC(-3/-3) 768 GAGCGG BstXI 101 CCATGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 828 CCAGCATTATGG BstYI 1268 AGATCC [GA]^GATC[CT] 1769 AGATCT CfoI 876 GCGC GCG^C 1713 GCGC DpnII 1269 GATC ^GATC 1770 GATC EaeI 110 TGGCCA [CT]^GGCC[GA] 474 CGGCCA EcoT14I 101 CCATGG C^C[AT][AT]GG 758 CCAAGG HhaI 876 GCGC GCG^C 1713 GCGC HinP1I 876 GCGC G^CGC 1713 GCGC MaeII 1613 ACGT A^CGT 1659 ACGT MboI 1269 GATC ^GATC 1770 GATC MflI 1268 AGATCC [GA]^GATC[CT] 1769 AGATCT MslI 162 CACCTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 832 CATTATGGTG NdeII 1269 GATC ^GATC 1770 GATC PflMI 645 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1161 CCAGGAGCTGG Sau3AI 1269 GATC ^GATC 1770 GATC StuI 504 AGGCCT AGG^CCT 1398 AGGCCT StyI 101 CCATGG C^C[AT][AT]GG 758 CCAAGG TaqI 308 TCGA T^CGA 1493 TCGA TthHB8I 308 TCGA T^CGA 1493 TCGA Van91I 645 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1161 CCAGGAGCTGG XhoII 1268 AGATCC [GA]^GATC[CT] 1769 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AciI AfaI AluI AlwNI ApaI AspHI AvaI AvaII BanII BbvI BciVI BcnI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstAPI BstF5I BstNI Cac8I Cfr10I Cfr13I DdeI DraII DsaI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeIII MboII MnlI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV PleI PpuMI PstI PvuII RsaI Sau96I ScrFI SfaNI SfcI SmlI TseI Tsp45I TspRI XcmI