Restriction map for pFN21AB8241
 (Circular) (Six-base) MAPSORT of: pFN21AB8241.seq  Check: 565  from: 1  to: 6097



pFN21AB8241  6097 bp

 With 182 enzymes: SgfI * 

                        August 16, 2010 14:42  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2384    2503    2384 
   Size:        119    5978
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3907     278 
   Size:         53      83     186     936    2371    2468

  Fragments arranged by size: 

                2468    2371     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2972    3440    1937 
   Size:       1035     468    4594

  Fragments arranged by size: 

                4594    1035     468
 
Acc65I G'GTAC_C

Cuts at:   2780    3418    2780 
   Size:        638    5459
 
AclI AA'CG_TT

Cuts at:   1563    4224    4597    1563 
   Size:       2661     373    3063

  Fragments arranged by size: 

                3063    2661     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2129    2234    2699    4160    5544    5812    1632 
   Size:        497     105     465    1461    1384     268    1917

  Fragments arranged by size: 

                1917    1461    1384     497     465     268     105
 
AfeI AGC'GCT

Cuts at:   2637    2637 
   Size:       6097
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5900
 
AflIII A'CryG_T

Cuts at:   1203    2586    4996    5973    1203 
   Size:       1383    2410     977    1327

  Fragments arranged by size: 

                2410    1383    1327     977
 
AgeI A'CCGG_T

Cuts at:   4928    4928 
   Size:       6097
 
AhdI GACnn_n'nnGTC

Cuts at:   4826    4826 
   Size:       6097
 
AleI CACnn'nnGTG

Cuts at:   1166    2726    1166 
   Size:       1560    4537
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    3378    1184 
   Size:       2194    3903
 
AlwNI CAG_nnn'CTG

Cuts at:   3316    3840    5412    3316 
   Size:        524    1572    4001

  Fragments arranged by size: 

                4001    1572     524
 
ApaI G_GGCC'C

Cuts at:   2223    2223 
   Size:       6097
 
ApaLI G'TGCA_C

Cuts at:   4154    5310    4154 
   Size:       1156    4941
 
ApoI r'AATT_y

Cuts at:   1493    2486    3406    3632    3784    5893    1493 
   Size:        993     920     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     993     920     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4651     160 
   Size:        872    3619    1606

  Fragments arranged by size: 

                3619    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6097
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3341    3422    1870 
   Size:         73    1398      81    4545

  Fragments arranged by size: 

                4545    1398      81      73
 
BamHI G'GATC_C

Cuts at:   2229    3427    3794    2229 
   Size:       1198     367    4532

  Fragments arranged by size: 

                4532    1198     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2372    2453    2780    2819    3418 
   Size:        325     217     627     585      81     327      39     599

Cuts at:   3418    4873     618 
   Size:       1455    1842

  Fragments arranged by size: 

                1842    1455     627     599     585     327     325     217
                  81      39
 
BanII G_rGCy'C

Cuts at:   1391    2223    3342    3416    1391 
   Size:        832    1119      74    4072

  Fragments arranged by size: 

                4072    1119     832      74
 
BbeI G_GCGC'C

Cuts at:   2457    2457 
   Size:       6097
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2124     928 
   Size:        971     225    4901

  Fragments arranged by size: 

                4901     971     225
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    4288    4322    1903 
   Size:         34      50      34    2267      34    3678

  Fragments arranged by size: 

                3678    2267      50      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3989    5199    3989 
   Size:       1210    4887
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       6097
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2009    2271    2537 
   Size:        107     122      71     631     605     337     262     266

Cuts at:   2537    3221    3386    4708     136 
   Size:        684     165    1322    1525

  Fragments arranged by size: 

                1525    1322     684     631     605     337     266     262
                 165     122     107      71
 
BglII A'GATC_T

Cuts at:   2391    6092    2391 
   Size:       3701    2396
 
BlpI GC'TnA_GC

Cuts at:   3514    3514 
   Size:       6097
 
Bme1580I G_kGCm'C

Cuts at:   1790    2223    2375    2889    3056    4158    5314    1790 
   Size:        433     152     514     167    1102    1156    2573

  Fragments arranged by size: 

                2573    1156    1102     514     433     167     152
 
BmgBI CAC'GTC

Cuts at:   1318    2587    2701    1318 
   Size:       1269     114    4714

  Fragments arranged by size: 

                4714    1269     114
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3828    4786     442 
   Size:        416      53      83     399      24    2411     958    1753

  Fragments arranged by size: 

                2411    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6097
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3250    3250 
   Size:       6097
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2176    4757    1239 
   Size:        937    2581    2579

  Fragments arranged by size: 

                2581    2579     937
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2279    4222    5087    5385    2279 
   Size:       1943     865     298    2991

  Fragments arranged by size: 

                2991    1943     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4760     882 
   Size:        617    3261    2219

  Fragments arranged by size: 

                3261    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3888     493 
   Size:        711    2684    2702

  Fragments arranged by size: 

                2702    2684     711
 
BsaBI GATnn'nnATC

Cuts at:   6091    6091 
   Size:       6097
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2454    3904    4286    4921 
   Size:         53      83     186     936     921    1450     382     635

Cuts at:   4921     275 
   Size:       1451

  Fragments arranged by size: 

                1451    1450     936     921     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   2121    4530    4928    5202    5349    5760    2121 
   Size:       2409     398     274     147     411    2458

  Fragments arranged by size: 

                2458    2409     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2864    2894    1178 
   Size:         30     165      30    1461      30    4381

  Fragments arranged by size: 

                4381    1461     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2642    2885    2888    3359    1186 
   Size:       1456     243       3     471    3924

  Fragments arranged by size: 

                3924    1456     471     243       3
 
BseYI C'CCAG_C

Cuts at:   2606    2915    2934    3366    5300    5796    2606 
   Size:        309      19     432    1934     496    2907

  Fragments arranged by size: 

                2907    1934     496     432     309      19
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    3053    3329    1163 
   Size:        573    1317     276    3931

  Fragments arranged by size: 

                3931    1317     573     276
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2146    3221    3225    3335    3547    4308 
   Size:        275     186     145    1075       4     110     212     761

Cuts at:   4308    5336    1540 
   Size:       1028    2301

  Fragments arranged by size: 

                2301    1075    1028     761     275     212     186     145
                 110       4
 
BsiHKAI G_wGCw'C

Cuts at:   3283    3342    4158    4243    5314    3283 
   Size:         59     816      85    1071    4066

  Fragments arranged by size: 

                4066    1071     816      85      59
 
BsmI GAATG_Cn'

Cuts at:   2563    3608    3701    2563 
   Size:       1045      93    4959

  Fragments arranged by size: 

                4959    1045      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2996    2996 
   Size:       6097
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2223    2375    2889    3056    3283    3342    3416 
   Size:        399     433     152     514     167     227      59      74

Cuts at:   3416    4158    4243    5314    1391 
   Size:        742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     514     433     399     227     167
                 152      85      74      59
 
BspEI T'CCGG_A

Cuts at:   5760    5760 
   Size:       6097
 
BspHI T'CATG_A

Cuts at:   2307    3986    5716    2307 
   Size:       1679    1730    2688

  Fragments arranged by size: 

                2688    1730    1679
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2384    2503    2531    3452     844 
   Size:       1540     119      28     921    3489

  Fragments arranged by size: 

                3489    1540     921     119      28
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3081    3349    3984    1394 
   Size:        429    1258     268     635    3507

  Fragments arranged by size: 

                3507    1258     635     429     268
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4592    4766      65 
   Size:       1183    3344     174    1396

  Fragments arranged by size: 

                3344    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3220    4741    4928    1700 
   Size:        110    1410    1521     187    2869

  Fragments arranged by size: 

                2869    1521    1410     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       6097
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6097
 
BssSI C'ACGA_G

Cuts at:   3131    4157    5169    3131 
   Size:       1026    1012    4059

  Fragments arranged by size: 

                4059    1026    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       6097
 
BstBI TT'CG_AA

Cuts at:   4901    4901 
   Size:       6097
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6097
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2341    2728    2341 
   Size:        387    5710
 
BstYI r'GATC_y

Cuts at:   1972    2205    2229    2391    3427    3794    4179    4196    5648 
   Size:        233      24     162    1036     367     385      17    1452

Cuts at:   5648    5734    5746    6092    1972 
   Size:         86      12     346    1977

  Fragments arranged by size: 

                1977    1452    1036     385     367     346     233     162
                  86      24      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2266    2448    2773     513 
   Size:        552    1201     182     325    3837

  Fragments arranged by size: 

                3837    1201     552     325     182
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    3088     487 
   Size:        683      56    1862    3496

  Fragments arranged by size: 

                3496    1862     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2757    3613    4407    4427    2757 
   Size:        856     794      20    4427

  Fragments arranged by size: 

                4427     856     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6097
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6062
 
DraI TTT'AAA

Cuts at:   3401    3401 
   Size:       6097
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5104     809 
   Size:        507    3788    1802

  Fragments arranged by size: 

                3788    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2143    2517    2730 
   Size:         54    1425      50     275      18     313     374     213

Cuts at:   2730    2842    3142    3218    3222    3332    3544    4433    6062 
   Size:        112     300      76       4     110     212     889    1629

Cuts at:   6062       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     889     374     313     300     275     213
                 212     112     110      76      54      50      43      18
                   4
 
EagI C'GGCC_G

Cuts at:   1537    1812    2143    3218    3222    3332    3544    1537 
   Size:        275     331    1075       4     110     212    4090

  Fragments arranged by size: 

                4090    1075     331     275     212     110       4
 
EarI CTCTTCn'nnn_

Cuts at:   1353    4027    1353 
   Size:       2674    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3311    4684    5058    5204     387 
   Size:       1064     138    1722    1373     374     146    1280

  Fragments arranged by size: 

                1722    1373    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   3340    3340 
   Size:       6097
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2129    2176    2234    2699    4160    4757    5544 
   Size:        393     497      47      58     465    1461     597     787

Cuts at:   5544    5812    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524    1461     787     597     497     465     393     268
                  58      47
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2213    2213 
   Size:       6097
 
EcoO109I rG'GnC_Cy

Cuts at:   2219    2610    2219 
   Size:        391    5706
 
EcoRI G'AATT_C

Cuts at:   2486    3406    3784    2486 
   Size:        920     378    4799

  Fragments arranged by size: 

                4799     920     378
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2320    2704    2320 
   Size:        384    5713
 
FseI GG_CCGG'CC

Cuts at:   3224    3224 
   Size:       6097
 
FspI TGC'GCA

Cuts at:   4603    4603 
   Size:       6097
 
HaeII r_GCGC'y

Cuts at:   1678    2277    2457    2639    5244    1678 
   Size:        599     180     182    2605    2531

  Fragments arranged by size: 

                2605    2531     599     182     180
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2864    2896    3265    3297    4738 
   Size:         32     135      32    1292      32     369      32    1441

Cuts at:   4738    4770    4812    4844    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1441    1292     369     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2851    3441    3687     669 
   Size:        988     281     913     590     246    3079

  Fragments arranged by size: 

                3079     988     913     590     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       6097
 
HpaI GTT'AAC

Cuts at:   3687    3687 
   Size:       6097
 
KasI G'GCGC_C

Cuts at:   2453    2453 
   Size:       6097
 
KpnI G_GTAC'C

Cuts at:   2784    3422    2784 
   Size:        638    5459
 
MfeI C'AATT_G

Cuts at:   3696    3696 
   Size:       6097
 
MluI A'CGCG_T

Cuts at:   5973    5973 
   Size:       6097
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    5211    5395    1305 
   Size:       3906     184    2007

  Fragments arranged by size: 

                3906    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2519    2732    2844    3144      10 
   Size:         54    1425     343     687     213     112     300    2963

  Fragments arranged by size: 

                2963    1425     687     343     300     213     112      54
            
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2726    4055    4414    4573     518 
   Size:        648    1560    1329     359     159    2042

  Fragments arranged by size: 

                2042    1560    1329     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2067    3059    3358    3513    4190    5338    5583    1606 
   Size:        461     992     299     155     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     992     677     461     299     245     155
            
 
NaeI GCC'GGC

Cuts at:   3222    3222 
   Size:       6097
 
NarI GG'CG_CC

Cuts at:   2454    2454 
   Size:       6097
 
NcoI C'CATG_G

Cuts at:    513    1065    2448     513 
   Size:        552    1383    4162

  Fragments arranged by size: 

                4162    1383     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       6097
 
NgoMIV G'CCGG_C

Cuts at:   3220    3220 
   Size:       6097
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6097
 
NotI GC'GGCC_GC

Cuts at:   3544    3544 
   Size:       6097
 
NspI r_CATG'y

Cuts at:   1132    1229    2915    3455    5000    1132 
   Size:         97    1686     540    1545    2229

  Fragments arranged by size: 

                2229    1686    1545     540      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       6097
 
PciI A'CATG_T

Cuts at:   4996    4996 
   Size:       6097
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       6097
 
PmeI GTTT'AAAC

Cuts at:   3401    3401 
   Size:       6097
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4166    4198    5679    5711    4166 
   Size:         32    1481      32    4552

  Fragments arranged by size: 

                4552    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6097
 
PsiI TTA'TAA

Cuts at:   3667    3667 
   Size:       6097
 
PspOMI G'GGCC_C

Cuts at:   2219    2219 
   Size:       6097
 
PspXI vC'TCGA_Gb

Cuts at:   3341    3341 
   Size:       6097
 
PstI C_TGCA'G

Cuts at:    830    2514    3449     830 
   Size:       1684     935    3478

  Fragments arranged by size: 

                3478    1684     935
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6097
 
PvuII CAG'CTG

Cuts at:   3059    3059 
   Size:       6097
 
SacI G_AGCT'C

Cuts at:   3342    3342 
   Size:       6097
 
SalI G'TCGA_C

Cuts at:   1936    3439    1936 
   Size:       1503    4594
 
SbfI CC_TGCA'GG

Cuts at:   3449    3449 
   Size:       6097
 
ScaI AGT'ACT

Cuts at:   4345    6057    4345 
   Size:       1712    4385
 
SexAI A'CCwGG_T

Cuts at:   2783    2783 
   Size:       6097
 
SfcI C'TryA_G

Cuts at:    826    1045    2510    3445    4580    5261    5452     826 
   Size:        219    1465     935    1135     681     191    1471

  Fragments arranged by size: 

                1471    1465    1135     935     681     219     191
 
SfoI GGC'GCC

Cuts at:   2455    2455 
   Size:       6097
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6097
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6097
 
SmaI CCC'GGG

Cuts at:   1872    3424    1872 
   Size:       1552    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2294    3341    4201    5102    5364     820 
   Size:        197     926     351    1047     860     901     262    1553

  Fragments arranged by size: 

                1553    1047     926     901     860     351     262     197
            
 
SnaBI TAC'GTA

Cuts at:    493    3888     493 
   Size:       3395    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6097
 
SphI G_CATG'C

Cuts at:   1132    2915    3455    1132 
   Size:       1783     540    3774

  Fragments arranged by size: 

                3774    1783     540
 
SspI AAT'ATT

Cuts at:      5      52    4021       5 
   Size:         47    3969    2081

  Fragments arranged by size: 

                3969    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2501    2531    3048    1517 
   Size:        984      30     517    4566

  Fragments arranged by size: 

                4566     984     517      30
 
StyI C'CwwG_G

Cuts at:    513    1065    2448    3536     513 
   Size:        552    1383    1088    3074

  Fragments arranged by size: 

                3074    1383    1088     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2098    3275    4124    4141    4294    4479    4924 
   Size:       1255     518    1177     849      17     153     185     445

Cuts at:   4924     325 
   Size:       1498

  Fragments arranged by size: 

                1498    1255    1177     849     518     445     185     153
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    4343    6055      96 
   Size:        274      80      33      51    1443    2366    1712     138

  Fragments arranged by size: 

                2366    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   6057    6089    6057 
   Size:         32    6065
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6097
 
XbaI T'CTAG_A

Cuts at:   3433    3433 
   Size:       6097
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2341    2512    2341 
   Size:        171    5926
 
XhoI C'TCGA_G

Cuts at:   1943    3341    1943 
   Size:       1398    4699
 
XmaI C'CCGG_G

Cuts at:   1870    3422    1870 
   Size:       1552    4545
 
XmnI GAAnn'nnTTC

Cuts at:   4226    4226 
   Size:       6097
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3905     276 
   Size:         53      83     186     936    2371    2468

  Fragments arranged by size: 

                2468    2371     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI     FseI     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NspI     PasI     PciI    PflMI     PmeI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PstI     PvuI    PvuII     SacI     SalI
     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AloI     AscI    AvrII     BaeI    BbvCI    BfrBI   Bpu10I    BsiWI
  BstZ17I   Bsu36I   DraIII    EcoRV    FspAI     NruI     NsiI     PacI
     PfoI     PmlI    PpuMI     PsrI    RsrII    SacII    SanDI     SapI
     SfiI     SrfI     SwaI
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