Restriction map for
pFN21AB8241
(Circular) (Six-base) MAPSORT of: pFN21AB8241.seq Check: 565 from: 1 to: 6097
pFN21AB8241 6097 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2384 2503 2384
Size: 119 5978
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3907 278
Size: 53 83 186 936 2371 2468
Fragments arranged by size:
2468 2371 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2972 3440 1937
Size: 1035 468 4594
Fragments arranged by size:
4594 1035 468
Acc65I G'GTAC_C
Cuts at: 2780 3418 2780
Size: 638 5459
AclI AA'CG_TT
Cuts at: 1563 4224 4597 1563
Size: 2661 373 3063
Fragments arranged by size:
3063 2661 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2129 2234 2699 4160 5544 5812 1632
Size: 497 105 465 1461 1384 268 1917
Fragments arranged by size:
1917 1461 1384 497 465 268 105
AfeI AGC'GCT
Cuts at: 2637 2637
Size: 6097
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5900
AflIII A'CryG_T
Cuts at: 1203 2586 4996 5973 1203
Size: 1383 2410 977 1327
Fragments arranged by size:
2410 1383 1327 977
AgeI A'CCGG_T
Cuts at: 4928 4928
Size: 6097
AhdI GACnn_n'nnGTC
Cuts at: 4826 4826
Size: 6097
AleI CACnn'nnGTG
Cuts at: 1166 2726 1166
Size: 1560 4537
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3378 1184
Size: 2194 3903
AlwNI CAG_nnn'CTG
Cuts at: 3316 3840 5412 3316
Size: 524 1572 4001
Fragments arranged by size:
4001 1572 524
ApaI G_GGCC'C
Cuts at: 2223 2223
Size: 6097
ApaLI G'TGCA_C
Cuts at: 4154 5310 4154
Size: 1156 4941
ApoI r'AATT_y
Cuts at: 1493 2486 3406 3632 3784 5893 1493
Size: 993 920 226 152 2109 1697
Fragments arranged by size:
2109 1697 993 920 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4651 160
Size: 872 3619 1606
Fragments arranged by size:
3619 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6097
AvaI C'yCGr_G
Cuts at: 1870 1943 3341 3422 1870
Size: 73 1398 81 4545
Fragments arranged by size:
4545 1398 81 73
BamHI G'GATC_C
Cuts at: 2229 3427 3794 2229
Size: 1198 367 4532
Fragments arranged by size:
4532 1198 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2372 2453 2780 2819 3418
Size: 325 217 627 585 81 327 39 599
Cuts at: 3418 4873 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 599 585 327 325 217
81 39
BanII G_rGCy'C
Cuts at: 1391 2223 3342 3416 1391
Size: 832 1119 74 4072
Fragments arranged by size:
4072 1119 832 74
BbeI G_GCGC'C
Cuts at: 2457 2457
Size: 6097
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2124 928
Size: 971 225 4901
Fragments arranged by size:
4901 971 225
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4288 4322 1903
Size: 34 50 34 2267 34 3678
Fragments arranged by size:
3678 2267 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3989 5199 3989
Size: 1210 4887
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6097
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2271 2537
Size: 107 122 71 631 605 337 262 266
Cuts at: 2537 3221 3386 4708 136
Size: 684 165 1322 1525
Fragments arranged by size:
1525 1322 684 631 605 337 266 262
165 122 107 71
BglII A'GATC_T
Cuts at: 2391 6092 2391
Size: 3701 2396
BlpI GC'TnA_GC
Cuts at: 3514 3514
Size: 6097
Bme1580I G_kGCm'C
Cuts at: 1790 2223 2375 2889 3056 4158 5314 1790
Size: 433 152 514 167 1102 1156 2573
Fragments arranged by size:
2573 1156 1102 514 433 167 152
BmgBI CAC'GTC
Cuts at: 1318 2587 2701 1318
Size: 1269 114 4714
Fragments arranged by size:
4714 1269 114
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3828 4786 442
Size: 416 53 83 399 24 2411 958 1753
Fragments arranged by size:
2411 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6097
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3250 3250
Size: 6097
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2176 4757 1239
Size: 937 2581 2579
Fragments arranged by size:
2581 2579 937
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2279 4222 5087 5385 2279
Size: 1943 865 298 2991
Fragments arranged by size:
2991 1943 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4760 882
Size: 617 3261 2219
Fragments arranged by size:
3261 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3888 493
Size: 711 2684 2702
Fragments arranged by size:
2702 2684 711
BsaBI GATnn'nnATC
Cuts at: 6091 6091
Size: 6097
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2454 3904 4286 4921
Size: 53 83 186 936 921 1450 382 635
Cuts at: 4921 275
Size: 1451
Fragments arranged by size:
1451 1450 936 921 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2121 4530 4928 5202 5349 5760 2121
Size: 2409 398 274 147 411 2458
Fragments arranged by size:
2458 2409 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2864 2894 1178
Size: 30 165 30 1461 30 4381
Fragments arranged by size:
4381 1461 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2642 2885 2888 3359 1186
Size: 1456 243 3 471 3924
Fragments arranged by size:
3924 1456 471 243 3
BseYI C'CCAG_C
Cuts at: 2606 2915 2934 3366 5300 5796 2606
Size: 309 19 432 1934 496 2907
Fragments arranged by size:
2907 1934 496 432 309 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3053 3329 1163
Size: 573 1317 276 3931
Fragments arranged by size:
3931 1317 573 276
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2146 3221 3225 3335 3547 4308
Size: 275 186 145 1075 4 110 212 761
Cuts at: 4308 5336 1540
Size: 1028 2301
Fragments arranged by size:
2301 1075 1028 761 275 212 186 145
110 4
BsiHKAI G_wGCw'C
Cuts at: 3283 3342 4158 4243 5314 3283
Size: 59 816 85 1071 4066
Fragments arranged by size:
4066 1071 816 85 59
BsmI GAATG_Cn'
Cuts at: 2563 3608 3701 2563
Size: 1045 93 4959
Fragments arranged by size:
4959 1045 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2996 2996
Size: 6097
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2223 2375 2889 3056 3283 3342 3416
Size: 399 433 152 514 167 227 59 74
Cuts at: 3416 4158 4243 5314 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 514 433 399 227 167
152 85 74 59
BspEI T'CCGG_A
Cuts at: 5760 5760
Size: 6097
BspHI T'CATG_A
Cuts at: 2307 3986 5716 2307
Size: 1679 1730 2688
Fragments arranged by size:
2688 1730 1679
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2384 2503 2531 3452 844
Size: 1540 119 28 921 3489
Fragments arranged by size:
3489 1540 921 119 28
BsrBI CCG'CTC
Cuts at: 1394 1823 3081 3349 3984 1394
Size: 429 1258 268 635 3507
Fragments arranged by size:
3507 1258 635 429 268
BsrDI GCAATG_nn'
Cuts at: 65 1248 4592 4766 65
Size: 1183 3344 174 1396
Fragments arranged by size:
3344 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3220 4741 4928 1700
Size: 110 1410 1521 187 2869
Fragments arranged by size:
2869 1521 1410 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6097
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6097
BssSI C'ACGA_G
Cuts at: 3131 4157 5169 3131
Size: 1026 1012 4059
Fragments arranged by size:
4059 1026 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6097
BstBI TT'CG_AA
Cuts at: 4901 4901
Size: 6097
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6097
BstXI CCAn_nnnn'nTGG
Cuts at: 2341 2728 2341
Size: 387 5710
BstYI r'GATC_y
Cuts at: 1972 2205 2229 2391 3427 3794 4179 4196 5648
Size: 233 24 162 1036 367 385 17 1452
Cuts at: 5648 5734 5746 6092 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1036 385 367 346 233 162
86 24 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2266 2448 2773 513
Size: 552 1201 182 325 3837
Fragments arranged by size:
3837 1201 552 325 182
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3088 487
Size: 683 56 1862 3496
Fragments arranged by size:
3496 1862 683 56
BtsI GCAGTG_nn'
Cuts at: 2757 3613 4407 4427 2757
Size: 856 794 20 4427
Fragments arranged by size:
4427 856 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6097
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6062
DraI TTT'AAA
Cuts at: 3401 3401
Size: 6097
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5104 809
Size: 507 3788 1802
Fragments arranged by size:
3788 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2143 2517 2730
Size: 54 1425 50 275 18 313 374 213
Cuts at: 2730 2842 3142 3218 3222 3332 3544 4433 6062
Size: 112 300 76 4 110 212 889 1629
Cuts at: 6062 8
Size: 43
Fragments arranged by size:
1629 1425 889 374 313 300 275 213
212 112 110 76 54 50 43 18
4
EagI C'GGCC_G
Cuts at: 1537 1812 2143 3218 3222 3332 3544 1537
Size: 275 331 1075 4 110 212 4090
Fragments arranged by size:
4090 1075 331 275 212 110 4
EarI CTCTTCn'nnn_
Cuts at: 1353 4027 1353
Size: 2674 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3311 4684 5058 5204 387
Size: 1064 138 1722 1373 374 146 1280
Fragments arranged by size:
1722 1373 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3340 3340
Size: 6097
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2129 2176 2234 2699 4160 4757 5544
Size: 393 497 47 58 465 1461 597 787
Cuts at: 5544 5812 1239
Size: 268 1524
Fragments arranged by size:
1524 1461 787 597 497 465 393 268
58 47
EcoNI CCTnn'n_nnAGG
Cuts at: 2213 2213
Size: 6097
EcoO109I rG'GnC_Cy
Cuts at: 2219 2610 2219
Size: 391 5706
EcoRI G'AATT_C
Cuts at: 2486 3406 3784 2486
Size: 920 378 4799
Fragments arranged by size:
4799 920 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2320 2704 2320
Size: 384 5713
FseI GG_CCGG'CC
Cuts at: 3224 3224
Size: 6097
FspI TGC'GCA
Cuts at: 4603 4603
Size: 6097
HaeII r_GCGC'y
Cuts at: 1678 2277 2457 2639 5244 1678
Size: 599 180 182 2605 2531
Fragments arranged by size:
2605 2531 599 182 180
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2864 2896 3265 3297 4738
Size: 32 135 32 1292 32 369 32 1441
Cuts at: 4738 4770 4812 4844 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1441 1292 369 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2851 3441 3687 669
Size: 988 281 913 590 246 3079
Fragments arranged by size:
3079 988 913 590 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6097
HpaI GTT'AAC
Cuts at: 3687 3687
Size: 6097
KasI G'GCGC_C
Cuts at: 2453 2453
Size: 6097
KpnI G_GTAC'C
Cuts at: 2784 3422 2784
Size: 638 5459
MfeI C'AATT_G
Cuts at: 3696 3696
Size: 6097
MluI A'CGCG_T
Cuts at: 5973 5973
Size: 6097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5211 5395 1305
Size: 3906 184 2007
Fragments arranged by size:
3906 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2519 2732 2844 3144 10
Size: 54 1425 343 687 213 112 300 2963
Fragments arranged by size:
2963 1425 687 343 300 213 112 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2726 4055 4414 4573 518
Size: 648 1560 1329 359 159 2042
Fragments arranged by size:
2042 1560 1329 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2067 3059 3358 3513 4190 5338 5583 1606
Size: 461 992 299 155 677 1148 245 2120
Fragments arranged by size:
2120 1148 992 677 461 299 245 155
NaeI GCC'GGC
Cuts at: 3222 3222
Size: 6097
NarI GG'CG_CC
Cuts at: 2454 2454
Size: 6097
NcoI C'CATG_G
Cuts at: 513 1065 2448 513
Size: 552 1383 4162
Fragments arranged by size:
4162 1383 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6097
NgoMIV G'CCGG_C
Cuts at: 3220 3220
Size: 6097
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6097
NotI GC'GGCC_GC
Cuts at: 3544 3544
Size: 6097
NspI r_CATG'y
Cuts at: 1132 1229 2915 3455 5000 1132
Size: 97 1686 540 1545 2229
Fragments arranged by size:
2229 1686 1545 540 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6097
PciI A'CATG_T
Cuts at: 4996 4996
Size: 6097
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6097
PmeI GTTT'AAAC
Cuts at: 3401 3401
Size: 6097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4166 4198 5679 5711 4166
Size: 32 1481 32 4552
Fragments arranged by size:
4552 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6097
PsiI TTA'TAA
Cuts at: 3667 3667
Size: 6097
PspOMI G'GGCC_C
Cuts at: 2219 2219
Size: 6097
PspXI vC'TCGA_Gb
Cuts at: 3341 3341
Size: 6097
PstI C_TGCA'G
Cuts at: 830 2514 3449 830
Size: 1684 935 3478
Fragments arranged by size:
3478 1684 935
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6097
PvuII CAG'CTG
Cuts at: 3059 3059
Size: 6097
SacI G_AGCT'C
Cuts at: 3342 3342
Size: 6097
SalI G'TCGA_C
Cuts at: 1936 3439 1936
Size: 1503 4594
SbfI CC_TGCA'GG
Cuts at: 3449 3449
Size: 6097
ScaI AGT'ACT
Cuts at: 4345 6057 4345
Size: 1712 4385
SexAI A'CCwGG_T
Cuts at: 2783 2783
Size: 6097
SfcI C'TryA_G
Cuts at: 826 1045 2510 3445 4580 5261 5452 826
Size: 219 1465 935 1135 681 191 1471
Fragments arranged by size:
1471 1465 1135 935 681 219 191
SfoI GGC'GCC
Cuts at: 2455 2455
Size: 6097
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6097
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6097
SmaI CCC'GGG
Cuts at: 1872 3424 1872
Size: 1552 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2294 3341 4201 5102 5364 820
Size: 197 926 351 1047 860 901 262 1553
Fragments arranged by size:
1553 1047 926 901 860 351 262 197
SnaBI TAC'GTA
Cuts at: 493 3888 493
Size: 3395 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6097
SphI G_CATG'C
Cuts at: 1132 2915 3455 1132
Size: 1783 540 3774
Fragments arranged by size:
3774 1783 540
SspI AAT'ATT
Cuts at: 5 52 4021 5
Size: 47 3969 2081
Fragments arranged by size:
3969 2081 47
StuI AGG'CCT
Cuts at: 1517 2501 2531 3048 1517
Size: 984 30 517 4566
Fragments arranged by size:
4566 984 517 30
StyI C'CwwG_G
Cuts at: 513 1065 2448 3536 513
Size: 552 1383 1088 3074
Fragments arranged by size:
3074 1383 1088 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2098 3275 4124 4141 4294 4479 4924
Size: 1255 518 1177 849 17 153 185 445
Cuts at: 4924 325
Size: 1498
Fragments arranged by size:
1498 1255 1177 849 518 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4343 6055 96
Size: 274 80 33 51 1443 2366 1712 138
Fragments arranged by size:
2366 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6057 6089 6057
Size: 32 6065
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6097
XbaI T'CTAG_A
Cuts at: 3433 3433
Size: 6097
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2341 2512 2341
Size: 171 5926
XhoI C'TCGA_G
Cuts at: 1943 3341 1943
Size: 1398 4699
XmaI C'CCGG_G
Cuts at: 1870 3422 1870
Size: 1552 4545
XmnI GAAnn'nnTTC
Cuts at: 4226 4226
Size: 6097
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3905 276
Size: 53 83 186 936 2371 2468
Fragments arranged by size:
2468 2371 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
BstZ17I Bsu36I DraIII EcoRV FspAI NruI NsiI PacI
PfoI PmlI PpuMI PsrI RsrII SacII SanDI SapI
SfiI SrfI SwaI