################################## # NO CUT ENZYMES ( 106 enzymes) # ################################## AatII AccI AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AseI AsnI AspEI AspI AviII BalI BamHI BbrPI BbsI BbvCI BciVI BfrI BglII BpuAI BsaAI BsaBI BseRI BsiWI BsmI BspDI BspHI BspLU11I BspMI BsrDI BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DrdI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HpaI KspI MamI MfeI MluI MluNI MscI MslI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SbfI ScaI SfcI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccIII 757 TCCGGA T^CCGGA AlwNI 538 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG AscI 348 GGCGCGCC GG^CGCGCC AvrII 435 CCTAGG C^CTAGG BcgI 123 GCAGAAGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 156 CCCGG CC^[GC]GG BlnI 435 CCTAGG C^CTAGG BlpI 594 GCTGAGC GC^T[ACGT]AGC Bpu1102I 594 GCTGAGC GC^T[ACGT]AGC BsaI 359 GAGACC GGTCTC(1/5) BseAI 757 TCCGGA T^CCGGA BsgI 610 GTGCAG GTGCAG(16/14) BsmBI 160 GAGACG CGTCTC(1/5) Bsp1407I 1105 TGTACA T^GTACA BspEI 757 TCCGGA T^CCGGA BsrGI 1105 TGTACA T^GTACA BssHII 349 GCGCGC G^CGCGC BstAPI 603 GCAGAGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 709 GGATG GGATG(2/0) BstXI 699 CCAGCGTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 730 GGATCT [GA]^GATC[CT] CelII 594 GCTGAGC GC^T[ACGT]AGC DraI 1177 TTTAAA TTT^AAA EagI 369 CGGCCG C^GGCCG EclXI 369 CGGCCG C^GGCCG Eco52I 369 CGGCCG C^GGCCG FokI 709 GGATG GGATG(9/13) HgaI 588 GACGC GACGC(5/10) HincII 780 GTTGAC GT[CT]^[GA]AC HindII 780 GTTGAC GT[CT]^[GA]AC HindIII 1063 AAGCTT A^AGCTT MaeII 320 ACGT A^CGT MflI 730 GGATCT [GA]^GATC[CT] MroI 757 TCCGGA T^CCGGA NaeI 599 GCCGGC GCC^GGC NciI 156 CCCGG CC^[GC]GG NgoMIV 599 GCCGGC G^CCGGC NspI 1005 GCATGT [GA]CATG^[CT] PaeR7I 131 CTCGAG C^TCGAG PpuMI 1029 GGGTCCT [GA]G^G[AT]CC[CT] SapI 189 GCTCTTC GCTCTTC(1/4) SspBI 1105 TGTACA T^GTACA StuI 674 AGGCCT AGG^CCT Tsp45I 572 GTCAC ^GT[GC]AC XcmI 344 CCACGGCGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 131 CTCGAG C^TCGAG XhoII 730 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcsI 1198 GAATTT [GA]^AATT[CT] 1401 AAATTT AcyI 6 GGCGCC G[GA]^CG[CT]C 340 GGCGCC AlwI 1210 GATCC GGATC(4/5) 730 GGATC ApoI 1198 GAATTT [GA]^AATT[CT] 1401 AAATTT Asp700I 954 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1198 GAATTTTTTC BbeI 6 GGCGCC GGCGC^C 340 GGCGCC BsaHI 6 GGCGCC G[GA]^CG[CT]C 340 GGCGCC BsaWI 757 TCCGGA [AT]^CCGG[AT] 927 ACCGGA BsiEI 195 CGACCG CG[GA][CT]^CG 369 CGGCCG BsrBI 308 GAGCGG CCGCTC(-3/-3) 366 GAGCGG BsrFI 35 GCCGGT [GA]^CCGG[CT] 599 GCCGGC Cfr10I 35 GCCGGT [GA]^CCGG[CT] 599 GCCGGC DraII 446 AGGCCCT [GA]G^G[ACGT]CC[CT] 1029 GGGTCCT DraIII 316 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG 541 CACCTGGTG EaeI 369 CGGCCG [CT]^GGCC[GA] 647 TGGCCG EarI 190 CTCTTC CTCTTC(1/4) 694 CTCTTC EcoO109I 446 AGGCCCT [GA]G^G[ACGT]CC[CT] 1029 GGGTCCT FauI 310 GCGGG CCCGC(4/6) 1026 GCGGG Hin1I 6 GGCGCC G[GA]^CG[CT]C 340 GGCGCC KasI 6 GGCGCC G^GCGCC 340 GGCGCC Ksp632I 190 CTCTTC CTCTTC(1/4) 694 CTCTTC NarI 6 GGCGCC GG^CGCC 340 GGCGCC PleI 253 GACTC GAGTC(4/5) 1298 GAGTC SexAI 542 ACCTGGT A^CC[AT]GGT 662 ACCAGGT SfaNI 710 GATGC GCATC(5/9) 1135 GATGC SfoI 6 GGCGCC GGC^GCC 340 GGCGCC XmnI 954 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1198 GAATTTTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### Acc65I AccII AciI AfaI AluI Asp718I AspHI AvaI AvaII BanI BanII BbvI BclI BfaI BglI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DsaI EcoRII EcoT14I FbaI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI KpnI MaeI MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PvuII RsaI SacI Sau3AI Sau96I ScrFI SmlI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I