(Circular) (Six-base) MAPSORT of: pFN21AE5604.seq Check: 5053 from: 1 to: 6136 pFN21AE5604 6136 bp With 182 enzymes: SgfI * December 8, 2014 13:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3946 278 Size: 53 83 186 936 2410 2468 Fragments arranged by size: 2468 2410 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3479 1937 Size: 1542 4594 Acc65I G'GTAC_C Cuts at: 2051 2532 2952 3457 2051 Size: 481 420 505 4730 Fragments arranged by size: 4730 505 481 420 AclI AA'CG_TT Cuts at: 1563 4263 4636 1563 Size: 2700 373 3063 Fragments arranged by size: 3063 2700 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2978 3413 4199 5583 5851 1632 Size: 1346 435 786 1384 268 1917 Fragments arranged by size: 1917 1384 1346 786 435 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5939 AflIII A'CryG_T Cuts at: 1203 5035 6012 1203 Size: 3832 977 1327 Fragments arranged by size: 3832 1327 977 AgeI A'CCGG_T Cuts at: 4967 4967 Size: 6136 AhdI GACnn_n'nnGTC Cuts at: 4865 4865 Size: 6136 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6136 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6136 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2799 2831 2799 Size: 32 6104 AlwNI CAG_nnn'CTG Cuts at: 2548 3879 5451 2548 Size: 1331 1572 3233 Fragments arranged by size: 3233 1572 1331 ApaLI G'TGCA_C Cuts at: 4193 5349 4193 Size: 1156 4980 ApoI r'AATT_y Cuts at: 1493 3203 3406 3445 3671 3823 5932 1493 Size: 1710 203 39 226 152 2109 1697 Fragments arranged by size: 2109 1710 1697 226 203 152 39 AscI GG'CGCG_CC Cuts at: 2354 2354 Size: 6136 AseI AT'TA_AT Cuts at: 160 1032 4690 160 Size: 872 3658 1606 Fragments arranged by size: 3658 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6136 AvaI C'yCGr_G Cuts at: 1870 1943 2101 2136 2368 2636 3273 3461 1870 Size: 73 158 35 232 268 637 188 4545 Fragments arranged by size: 4545 637 268 232 188 158 73 35 AvrII C'CTAG_G Cuts at: 2440 2440 Size: 6136 BamHI G'GATC_C Cuts at: 3466 3833 3466 Size: 367 5769 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2011 2051 2345 2532 2952 Size: 325 217 627 224 40 294 187 420 Cuts at: 2952 3457 4912 618 Size: 505 1455 1842 Fragments arranged by size: 1842 1455 627 505 420 325 294 224 217 187 40 BanII G_rGCy'C Cuts at: 1391 2187 2196 2434 2636 3455 1391 Size: 796 9 238 202 819 4072 Fragments arranged by size: 4072 819 796 238 202 9 BbeI G_GCGC'C Cuts at: 2015 2349 2015 Size: 334 5802 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5165 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2117 2151 4327 4361 1903 Size: 34 180 34 2176 34 3678 Fragments arranged by size: 3678 2176 180 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4028 5238 4028 Size: 1210 4926 BclI T'GATC_A Cuts at: 1548 2740 2992 3160 1548 Size: 1192 252 168 4524 Fragments arranged by size: 4524 1192 252 168 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2083 2459 Size: 107 122 71 631 605 337 74 376 Cuts at: 2459 2640 3028 4747 136 Size: 181 388 1719 1525 Fragments arranged by size: 1719 1525 631 605 388 376 337 181 122 107 74 71 BglII A'GATC_T Cuts at: 6131 6131 Size: 6136 BlpI GC'TnA_GC Cuts at: 2600 3553 2600 Size: 953 5183 Bme1580I G_kGCm'C Cuts at: 1790 4197 5353 1790 Size: 2407 1156 2573 Fragments arranged by size: 2573 2407 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6136 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3867 4825 442 Size: 416 53 83 399 24 2450 958 1753 Fragments arranged by size: 2450 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6136 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2420 2660 2811 2859 3167 4796 1239 Size: 1181 240 151 48 308 1629 2579 Fragments arranged by size: 2579 1629 1181 308 240 151 48 Bpu10I CC'TnA_GC Cuts at: 2109 2109 Size: 6136 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2468 2612 3353 4261 5126 5424 2468 Size: 144 741 908 865 298 3180 Fragments arranged by size: 3180 908 865 741 298 144 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2358 4799 882 Size: 617 859 2441 2219 Fragments arranged by size: 2441 2219 859 617 BsaAI yAC'GTr Cuts at: 493 1204 3927 493 Size: 711 2723 2702 Fragments arranged by size: 2723 2702 711 BsaBI GATnn'nnATC Cuts at: 6130 6130 Size: 6136 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 2346 3943 4325 Size: 53 83 186 936 479 334 1597 382 Cuts at: 4325 4960 275 Size: 635 1451 Fragments arranged by size: 1597 1451 936 635 479 382 334 186 83 53 BsaWI w'CCGG_w Cuts at: 2762 2932 4569 4967 5241 5388 5799 2762 Size: 170 1637 398 274 147 411 3099 Fragments arranged by size: 3099 1637 411 398 274 170 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2784 2814 3140 3170 1178 Size: 30 165 30 1381 30 326 30 4144 Fragments arranged by size: 4144 1381 326 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6136 BseYI C'CCAG_C Cuts at: 2290 2728 2848 5339 5835 2290 Size: 438 120 2491 496 2591 Fragments arranged by size: 2591 2491 496 438 120 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2636 1163 Size: 573 900 4663 Fragments arranged by size: 4663 900 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2203 2377 3586 4347 5375 1540 Size: 275 186 202 174 1209 761 1028 2301 Fragments arranged by size: 2301 1209 1028 761 275 202 186 174 BsiHKAI G_wGCw'C Cuts at: 2187 2196 2434 2676 4197 4282 5353 2187 Size: 9 238 242 1521 85 1071 2970 Fragments arranged by size: 2970 1521 1071 242 238 85 9 BsmI GAATG_Cn' Cuts at: 3647 3740 3647 Size: 93 6043 BsmBI CGTCTCn'nnnn_ Cuts at: 2159 2159 Size: 6136 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2187 2196 2434 2636 2676 3455 4197 Size: 399 397 9 238 202 40 779 742 Cuts at: 4197 4282 5353 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 779 742 399 397 238 202 85 40 9 BspEI T'CCGG_A Cuts at: 2762 5799 2762 Size: 3037 3099 BspHI T'CATG_A Cuts at: 4025 5755 4025 Size: 1730 4406 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3491 844 Size: 2647 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2315 2373 4023 1394 Size: 429 492 58 1650 3507 Fragments arranged by size: 3507 1650 492 429 58 BsrDI GCAATG_nn' Cuts at: 65 1248 4631 4805 65 Size: 1183 3383 174 1396 Fragments arranged by size: 3383 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2040 2604 4780 4967 1700 Size: 110 230 564 2176 187 2869 Fragments arranged by size: 2869 2176 564 230 187 110 BsrGI T'GTAC_A Cuts at: 96 3110 96 Size: 3014 3122 BssHII G'CGCG_C Cuts at: 1925 2354 1925 Size: 429 5707 BssSI C'ACGA_G Cuts at: 4196 5208 4196 Size: 1012 5124 BstAPI GCAn_nnn'nTGC Cuts at: 794 2614 794 Size: 1820 4316 BstBI TT'CG_AA Cuts at: 4940 4940 Size: 6136 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6136 BstXI CCAn_nnnn'nTGG Cuts at: 2711 2711 Size: 6136 BstYI r'GATC_y Cuts at: 1972 2735 3466 3833 4218 4235 5687 5773 5785 Size: 763 731 367 385 17 1452 86 12 Cuts at: 5785 6131 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 763 731 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2203 2250 2349 513 Size: 552 939 199 47 99 4300 Fragments arranged by size: 4300 939 552 199 99 47 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5397 Fragments arranged by size: 5397 683 56 BtsI GCAGTG_nn' Cuts at: 2574 3652 4446 4466 2574 Size: 1078 794 20 4244 Fragments arranged by size: 4244 1078 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6136 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6101 DraI TTT'AAA Cuts at: 3184 3440 3184 Size: 256 5880 DraIII CAC_nnn'GTG Cuts at: 2326 2551 2326 Size: 225 5911 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5143 809 Size: 507 3827 1802 Fragments arranged by size: 3827 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2374 2652 3583 Size: 54 1425 50 275 18 544 278 931 Cuts at: 3583 4472 6101 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 931 889 544 278 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2374 3583 1537 Size: 275 562 1209 4090 Fragments arranged by size: 4090 1209 562 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2201 2705 4066 1353 Size: 848 504 1361 3423 Fragments arranged by size: 3423 1361 848 504 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2748 3315 4723 5097 5243 387 Size: 1064 138 1159 567 1408 374 146 1280 Fragments arranged by size: 1408 1280 1159 1064 567 374 146 138 EcoICRI GAG'CTC Cuts at: 2185 2194 2432 2185 Size: 9 238 5889 Fragments arranged by size: 5889 238 9 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2420 2660 2811 2859 2978 3167 3413 Size: 393 788 240 151 48 119 189 246 Cuts at: 3413 4199 4796 5583 5851 1239 Size: 786 597 787 268 1524 Fragments arranged by size: 1524 788 787 786 597 393 268 246 240 189 151 119 48 EcoO109I rG'GnC_Cy Cuts at: 2452 3035 2452 Size: 583 5553 EcoRI G'AATT_C Cuts at: 3445 3823 3445 Size: 378 5758 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2173 2173 Size: 6136 FspI TGC'GCA Cuts at: 4642 4642 Size: 6136 HaeII r_GCGC'y Cuts at: 1678 2015 2109 2349 2399 2494 5283 1678 Size: 337 94 240 50 95 2789 2531 Fragments arranged by size: 2789 2531 337 240 95 94 50 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2477 2509 2706 2738 2879 Size: 32 135 32 905 32 197 32 141 Cuts at: 2879 2911 3140 3172 4777 4809 4851 4883 1373 Size: 32 229 32 1605 32 42 32 2626 Fragments arranged by size: 2626 1605 905 229 197 141 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2787 3480 3726 669 Size: 988 281 849 693 246 3079 Fragments arranged by size: 3079 988 849 693 281 246 HindIII A'AGCT_T Cuts at: 748 3068 748 Size: 2320 3816 HpaI GTT'AAC Cuts at: 3726 3726 Size: 6136 KasI G'GCGC_C Cuts at: 2011 2345 2011 Size: 334 5802 KpnI G_GTAC'C Cuts at: 2055 2536 2956 3461 2055 Size: 481 420 505 4730 Fragments arranged by size: 4730 505 481 420 MfeI C'AATT_G Cuts at: 3735 3735 Size: 6136 MluI A'CGCG_T Cuts at: 6012 6012 Size: 6136 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3334 5250 5434 1305 Size: 2029 1916 184 2007 Fragments arranged by size: 2029 2007 1916 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4314 Fragments arranged by size: 4314 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4094 4453 4612 518 Size: 648 2928 359 159 2042 Fragments arranged by size: 2928 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2620 2728 3007 3552 4229 5377 5622 1606 Size: 1014 108 279 545 677 1148 245 2120 Fragments arranged by size: 2120 1148 1014 677 545 279 245 108 NaeI GCC'GGC Cuts at: 2606 2606 Size: 6136 NarI GG'CG_CC Cuts at: 2012 2346 2012 Size: 334 5802 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4645 Fragments arranged by size: 4645 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6136 NgoMIV G'CCGG_C Cuts at: 2604 2604 Size: 6136 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6136 NotI GC'GGCC_GC Cuts at: 3583 3583 Size: 6136 NspI r_CATG'y Cuts at: 1132 1229 3014 3494 5039 1132 Size: 97 1785 480 1545 2229 Fragments arranged by size: 2229 1785 1545 480 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6136 PciI A'CATG_T Cuts at: 5035 5035 Size: 6136 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6136 PfoI T'CCnGG_A Cuts at: 2160 3217 2160 Size: 1057 5079 PmeI GTTT'AAAC Cuts at: 3440 3440 Size: 6136 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4205 4237 5718 5750 4205 Size: 32 1481 32 4591 Fragments arranged by size: 4591 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3035 3035 Size: 6136 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6136 PsiI TTA'TAA Cuts at: 3419 3706 3419 Size: 287 5849 PspXI vC'TCGA_Gb Cuts at: 2136 2136 Size: 6136 PstI C_TGCA'G Cuts at: 830 3488 830 Size: 2658 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6136 PvuII CAG'CTG Cuts at: 2620 2728 3007 2620 Size: 108 279 5749 Fragments arranged by size: 5749 279 108 SacI G_AGCT'C Cuts at: 2187 2196 2434 2187 Size: 9 238 5889 Fragments arranged by size: 5889 238 9 SalI G'TCGA_C Cuts at: 1936 3478 1936 Size: 1542 4594 SapI GCTCTTCn'nnn_ Cuts at: 2201 2201 Size: 6136 SbfI CC_TGCA'GG Cuts at: 3488 3488 Size: 6136 ScaI AGT'ACT Cuts at: 4384 6096 4384 Size: 1712 4424 SexAI A'CCwGG_T Cuts at: 2547 2667 2547 Size: 120 6016 SfcI C'TryA_G Cuts at: 826 1045 3484 4619 5300 5491 826 Size: 219 2439 1135 681 191 1471 Fragments arranged by size: 2439 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2013 2347 2013 Size: 334 5802 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6136 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6136 SmaI CCC'GGG Cuts at: 1872 3463 1872 Size: 1591 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2136 2447 2627 3368 4240 5141 Size: 197 926 193 311 180 741 872 901 Cuts at: 5141 5403 820 Size: 262 1553 Fragments arranged by size: 1553 926 901 872 741 311 262 197 193 180 SnaBI TAC'GTA Cuts at: 493 3927 493 Size: 3434 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6136 SphI G_CATG'C Cuts at: 1132 3494 1132 Size: 2362 3774 SspI AAT'ATT Cuts at: 5 52 4060 5 Size: 47 4008 2081 Fragments arranged by size: 4008 2081 47 StuI AGG'CCT Cuts at: 1517 2681 1517 Size: 1164 4972 StyI C'CwwG_G Cuts at: 513 1065 2004 2079 2382 2440 3575 513 Size: 552 939 75 303 58 1135 3074 Fragments arranged by size: 3074 1135 939 552 303 75 58 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4163 4180 4333 4518 4963 325 Size: 1255 2583 17 153 185 445 1498 Fragments arranged by size: 2583 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2831 3110 4382 Size: 274 80 33 51 1443 854 279 1272 Cuts at: 4382 6094 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1272 854 279 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6096 6128 6096 Size: 32 6104 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6136 XbaI T'CTAG_A Cuts at: 3472 3472 Size: 6136 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2356 2356 Size: 6136 XhoI C'TCGA_G Cuts at: 1943 2136 1943 Size: 193 5943 XmaI C'CCGG_G Cuts at: 1870 3461 1870 Size: 1591 4545 XmnI GAAnn'nnTTC Cuts at: 2963 3207 4265 2963 Size: 244 1058 4834 Fragments arranged by size: 4834 1058 244 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3944 276 Size: 53 83 186 936 2410 2468 Fragments arranged by size: 2468 2410 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI BaeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI NruI NsiI PacI PmlI PspOMI PsrI RsrII SacII SanDI SfiI SrfI SwaI