(Linear) (Six-base) MAPSORT of: KIEE5604.seq Check: 8751 from: 1 to: 1431 KIEE5604.orf With 182 enzymes: SgfI * December 5, 2014 13:02 .. Acc65I G'GTAC_C Cuts at: 0 46 527 947 1431 Size: 46 481 420 484 Fragments arranged by size: 484 481 420 46 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 973 1408 1431 Size: 973 435 23 Fragments arranged by size: 973 435 23 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 794 826 1431 Size: 794 32 605 Fragments arranged by size: 794 605 32 AlwNI CAG_nnn'CTG Cuts at: 0 543 1431 Size: 543 888 ApoI r'AATT_y Cuts at: 0 1198 1401 1431 Size: 1198 203 30 Fragments arranged by size: 1198 203 30 AscI GG'CGCG_CC Cuts at: 0 349 1431 Size: 349 1082 AvaI C'yCGr_G Cuts at: 0 96 131 363 631 1268 1431 Size: 96 35 232 268 637 163 Fragments arranged by size: 637 268 232 163 96 35 AvrII C'CTAG_G Cuts at: 0 435 1431 Size: 435 996 BanI G'GyrC_C Cuts at: 0 6 46 340 527 947 1431 Size: 6 40 294 187 420 484 Fragments arranged by size: 484 420 294 187 40 6 BanII G_rGCy'C Cuts at: 0 182 191 429 631 1431 Size: 182 9 238 202 800 Fragments arranged by size: 800 238 202 182 9 BbeI G_GCGC'C Cuts at: 0 10 344 1431 Size: 10 334 1087 Fragments arranged by size: 1087 334 10 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 112 146 1431 Size: 112 34 1285 Fragments arranged by size: 1285 112 34 BclI T'GATC_A Cuts at: 0 735 987 1155 1431 Size: 735 252 168 276 Fragments arranged by size: 735 276 252 168 BglI GCCn_nnn'nGGC Cuts at: 0 78 454 635 1023 1431 Size: 78 376 181 388 408 Fragments arranged by size: 408 388 376 181 78 BlpI GC'TnA_GC Cuts at: 0 595 1431 Size: 595 836 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 415 655 806 854 1162 1431 Size: 415 240 151 48 308 269 Fragments arranged by size: 415 308 269 240 151 48 Bpu10I CC'TnA_GC Cuts at: 0 104 1431 Size: 104 1327 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 463 607 1348 1431 Size: 463 144 741 83 Fragments arranged by size: 741 463 144 83 BsaI GGTCTCn'nnnn_ Cuts at: 0 353 1431 Size: 353 1078 BsaHI Gr'CG_yC Cuts at: 0 7 341 1431 Size: 7 334 1090 Fragments arranged by size: 1090 334 7 BsaWI w'CCGG_w Cuts at: 0 757 927 1431 Size: 757 170 504 Fragments arranged by size: 757 504 170 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 779 809 1135 1165 1431 Size: 779 30 326 30 266 Fragments arranged by size: 779 326 266 30 30 BseYI C'CCAG_C Cuts at: 0 285 723 843 1431 Size: 285 438 120 588 Fragments arranged by size: 588 438 285 120 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 631 1431 Size: 631 800 BsiEI CG_ry'CG Cuts at: 0 198 372 1431 Size: 198 174 1059 Fragments arranged by size: 1059 198 174 BsiHKAI G_wGCw'C Cuts at: 0 182 191 429 671 1431 Size: 182 9 238 242 760 Fragments arranged by size: 760 242 238 182 9 BsmBI CGTCTCn'nnnn_ Cuts at: 0 154 1431 Size: 154 1277 Bsp1286I G_dGCh'C Cuts at: 0 182 191 429 631 671 1431 Size: 182 9 238 202 40 760 Fragments arranged by size: 760 238 202 182 40 9 BspEI T'CCGG_A Cuts at: 0 757 1431 Size: 757 674 BsrBI CCG'CTC Cuts at: 0 310 368 1431 Size: 310 58 1063 Fragments arranged by size: 1063 310 58 BsrFI r'CCGG_y Cuts at: 0 35 599 1431 Size: 35 564 832 Fragments arranged by size: 832 564 35 BsrGI T'GTAC_A Cuts at: 0 1105 1431 Size: 1105 326 BssHII G'CGCG_C Cuts at: 0 349 1431 Size: 349 1082 BstAPI GCAn_nnn'nTGC Cuts at: 0 609 1431 Size: 609 822 BstXI CCAn_nnnn'nTGG Cuts at: 0 706 1431 Size: 706 725 BstYI r'GATC_y Cuts at: 0 730 1431 Size: 730 701 BtgI C'CryG_G Cuts at: 0 198 245 344 1431 Size: 198 47 99 1087 Fragments arranged by size: 1087 198 99 47 BtsI GCAGTG_nn' Cuts at: 0 569 1431 Size: 569 862 DraI TTT'AAA Cuts at: 0 1179 1431 Size: 1179 252 DraIII CAC_nnn'GTG Cuts at: 0 321 546 1431 Size: 321 225 885 Fragments arranged by size: 885 321 225 EaeI y'GGCC_r Cuts at: 0 369 647 1431 Size: 369 278 784 Fragments arranged by size: 784 369 278 EagI C'GGCC_G Cuts at: 0 369 1431 Size: 369 1062 EarI CTCTTCn'nnn_ Cuts at: 0 196 700 1431 Size: 196 504 731 Fragments arranged by size: 731 504 196 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 743 1310 1431 Size: 743 567 121 Fragments arranged by size: 743 567 121 EcoICRI GAG'CTC Cuts at: 0 180 189 427 1431 Size: 180 9 238 1004 Fragments arranged by size: 1004 238 180 9 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 415 655 806 854 973 1162 1408 1431 Size: 415 240 151 48 119 189 246 23 Fragments arranged by size: 415 246 240 189 151 119 48 23 EcoO109I rG'GnC_Cy Cuts at: 0 447 1030 1431 Size: 447 583 401 Fragments arranged by size: 583 447 401 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 168 1431 Size: 168 1263 HaeII r_GCGC'y Cuts at: 0 10 104 344 394 489 1431 Size: 10 94 240 50 95 942 Fragments arranged by size: 942 240 95 94 50 10 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 472 504 701 733 874 906 1135 1167 Size: 472 32 197 32 141 32 229 32 Cuts at: 1167 1431 Size: 264 Fragments arranged by size: 472 264 229 197 141 32 32 32 32 HincII GTy'rAC Cuts at: 0 782 1431 Size: 782 649 HindIII A'AGCT_T Cuts at: 0 1063 1431 Size: 1063 368 KasI G'GCGC_C Cuts at: 0 6 340 1431 Size: 6 334 1091 Fragments arranged by size: 1091 334 6 KpnI G_GTAC'C Cuts at: 0 50 531 951 1431 Size: 50 481 420 480 Fragments arranged by size: 481 480 420 50 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1329 1431 Size: 1329 102 MspA1I CmG'CkG Cuts at: 0 615 723 1002 1431 Size: 615 108 279 429 Fragments arranged by size: 615 429 279 108 NaeI GCC'GGC Cuts at: 0 601 1431 Size: 601 830 NarI GG'CG_CC Cuts at: 0 7 341 1431 Size: 7 334 1090 Fragments arranged by size: 1090 334 7 NgoMIV G'CCGG_C Cuts at: 0 599 1431 Size: 599 832 NspI r_CATG'y Cuts at: 0 1009 1431 Size: 1009 422 PfoI T'CCnGG_A Cuts at: 0 155 1212 1431 Size: 155 1057 219 Fragments arranged by size: 1057 219 155 PpuMI rG'GwC_Cy Cuts at: 0 1030 1431 Size: 1030 401 PsiI TTA'TAA Cuts at: 0 1414 1431 Size: 1414 17 PspXI vC'TCGA_Gb Cuts at: 0 131 1431 Size: 131 1300 PvuII CAG'CTG Cuts at: 0 615 723 1002 1431 Size: 615 108 279 429 Fragments arranged by size: 615 429 279 108 SacI G_AGCT'C Cuts at: 0 182 191 429 1431 Size: 182 9 238 1002 Fragments arranged by size: 1002 238 182 9 SapI GCTCTTCn'nnn_ Cuts at: 0 196 1431 Size: 196 1235 SexAI A'CCwGG_T Cuts at: 0 542 662 1431 Size: 542 120 769 Fragments arranged by size: 769 542 120 SfoI GGC'GCC Cuts at: 0 8 342 1431 Size: 8 334 1089 Fragments arranged by size: 1089 334 8 SmlI C'TyrA_G Cuts at: 0 131 442 622 1363 1431 Size: 131 311 180 741 68 Fragments arranged by size: 741 311 180 131 68 StuI AGG'CCT Cuts at: 0 676 1431 Size: 676 755 StyI C'CwwG_G Cuts at: 0 74 377 435 1431 Size: 74 303 58 996 Fragments arranged by size: 996 303 74 58 TatI w'GTAC_w Cuts at: 0 826 1105 1431 Size: 826 279 326 Fragments arranged by size: 826 326 279 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 351 1431 Size: 351 1080 XhoI C'TCGA_G Cuts at: 0 131 1431 Size: 131 1300 XmnI GAAnn'nnTTC Cuts at: 0 958 1202 1431 Size: 958 244 229 Fragments arranged by size: 958 244 229 Enzymes that do cut: Acc65I AcuI AloI AlwNI ApoI AscI AvaI AvrII BanI BanII BbeI BcgI BclI BglI BlpI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BsrBI BsrFI BsrGI BssHII BstAPI BstXI BstYI BtgI BtsI DraI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HincII HindIII KasI KpnI MmeI MspA1I NaeI NarI NgoMIV NspI PfoI PpuMI PsiI PspXI PvuII SacI SapI SexAI SfoI SmlI StuI StyI TatI XcmI XhoI XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AgeI AhdI AleI AlfI ApaI ApaLI AseI AsiSI BaeI BamHI BbsI BbvCI BciVI BfrBI BglII Bme1580I BmgBI BmrI BmtI BplI BsaAI BsaBI BseRI BsiWI BsmI BspHI BspMI BsrDI BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DrdI EcoNI EcoRI EcoRV FseI FspI FspAI HpaI MfeI MluI MscI MslI NcoI NdeI NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PspOMI PsrI PstI PvuI RsrII SacII SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII TstI Tth111I XbaI XmaI ZraI