(Circular) (Six-base) MAPSORT of: pFN21AE4396.seq Check: 6526 from: 1 to: 5956 pFN21AE4396 5956 bp With 182 enzymes: SgfI * July 6, 2012 17:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2378 2378 Size: 5956 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2091 3766 278 Size: 53 83 186 936 555 1675 2468 Fragments arranged by size: 2468 1675 936 555 186 83 53 AccI GT'mk_AC Cuts at: 1937 3299 1937 Size: 1362 4594 Acc65I G'GTAC_C Cuts at: 2769 3277 2769 Size: 508 5448 AclI AA'CG_TT Cuts at: 1563 4083 4456 1563 Size: 2520 373 3063 Fragments arranged by size: 3063 2520 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4019 5403 5671 1632 Size: 2387 1384 268 1917 Fragments arranged by size: 2387 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5759 AflIII A'CryG_T Cuts at: 1203 4855 5832 1203 Size: 3652 977 1327 Fragments arranged by size: 3652 1327 977 AgeI A'CCGG_T Cuts at: 4787 4787 Size: 5956 AhdI GACnn_n'nnGTC Cuts at: 2853 4685 2853 Size: 1832 4124 AleI CACnn'nnGTG Cuts at: 1166 2111 1166 Size: 945 5011 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5956 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2967 2999 3209 3241 2967 Size: 32 210 32 5682 Fragments arranged by size: 5682 210 32 32 AlwNI CAG_nnn'CTG Cuts at: 2606 2619 2704 2896 3699 5271 2606 Size: 13 85 192 803 1572 3291 Fragments arranged by size: 3291 1572 803 192 85 13 ApaI G_GGCC'C Cuts at: 2255 2255 Size: 5956 ApaLI G'TGCA_C Cuts at: 4013 5169 4013 Size: 1156 4800 ApoI r'AATT_y Cuts at: 1493 2282 3265 3491 3643 5752 1493 Size: 789 983 226 152 2109 1697 Fragments arranged by size: 2109 1697 983 789 226 152 AseI AT'TA_AT Cuts at: 160 1032 4510 160 Size: 872 3478 1606 Fragments arranged by size: 3478 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 AvaI C'yCGr_G Cuts at: 1870 1943 2010 3151 3281 1870 Size: 73 67 1141 130 4545 Fragments arranged by size: 4545 1141 130 73 67 AvrII C'CTAG_G Cuts at: 2956 2956 Size: 5956 BamHI G'GATC_C Cuts at: 3232 3286 3653 3232 Size: 54 367 5535 Fragments arranged by size: 5535 367 54 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2101 2113 2161 2578 2629 Size: 325 217 627 314 12 48 417 51 Cuts at: 2629 2769 2885 3277 4732 618 Size: 140 116 392 1455 1842 Fragments arranged by size: 1842 1455 627 417 392 325 314 217 140 116 51 48 12 BanII G_rGCy'C Cuts at: 1391 2255 2826 2965 3275 1391 Size: 864 571 139 310 4072 Fragments arranged by size: 4072 864 571 310 139 BbeI G_GCGC'C Cuts at: 2105 2889 2105 Size: 784 5172 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2227 928 Size: 971 328 4657 Fragments arranged by size: 4657 971 328 BbvCI CC'TCA_GC Cuts at: 2327 2451 2697 3175 2327 Size: 124 246 478 5108 Fragments arranged by size: 5108 478 246 124 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2560 2594 4147 4181 1903 Size: 34 623 34 1553 34 3678 Fragments arranged by size: 3678 1553 623 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2752 3848 5058 2752 Size: 1096 1210 3650 Fragments arranged by size: 3650 1210 1096 BclI T'GATC_A Cuts at: 1548 1548 Size: 5956 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4567 136 Size: 107 122 71 631 605 2895 1525 Fragments arranged by size: 2895 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5951 5951 Size: 5956 BlpI GC'TnA_GC Cuts at: 3373 3373 Size: 5956 Bme1580I G_kGCm'C Cuts at: 1790 2118 2166 2255 2490 2634 3251 4017 5173 Size: 328 48 89 235 144 617 766 1156 Cuts at: 5173 1790 Size: 2573 Fragments arranged by size: 2573 1156 766 617 328 235 144 89 48 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5956 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2309 3000 3687 Size: 416 53 83 399 24 892 691 687 Cuts at: 3687 4645 442 Size: 958 1753 Fragments arranged by size: 1753 958 892 691 687 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5956 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3238 3238 Size: 5956 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2496 4616 1239 Size: 1257 2120 2579 Fragments arranged by size: 2579 2120 1257 Bpu10I CC'TnA_GC Cuts at: 2327 2451 2697 3175 2327 Size: 124 246 478 5108 Fragments arranged by size: 5108 478 246 124 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2620 4081 4946 5244 2620 Size: 1461 865 298 3332 Fragments arranged by size: 3332 1461 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4619 882 Size: 617 3120 2219 Fragments arranged by size: 3120 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3747 493 Size: 711 2543 2702 Fragments arranged by size: 2702 2543 711 BsaBI GATnn'nnATC Cuts at: 5950 5950 Size: 5956 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2088 2102 2886 3763 Size: 53 83 186 936 555 14 784 877 Cuts at: 3763 4145 4780 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 877 784 635 555 382 186 83 53 14 BsaWI w'CCGG_w Cuts at: 4389 4787 5061 5208 5619 4389 Size: 398 274 147 411 4726 Fragments arranged by size: 4726 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2469 2499 1178 Size: 30 165 30 1066 30 4635 Fragments arranged by size: 4635 1066 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5956 BseYI C'CCAG_C Cuts at: 2255 2889 3079 5159 5655 2255 Size: 634 190 2080 496 2556 Fragments arranged by size: 2556 2080 634 496 190 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2117 1163 Size: 573 381 5002 Fragments arranged by size: 5002 573 381 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3406 4167 5195 1540 Size: 275 186 1405 761 1028 2301 Fragments arranged by size: 2301 1405 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2679 2846 2965 3015 4017 4102 5173 2679 Size: 167 119 50 1002 85 1071 3462 Fragments arranged by size: 3462 1071 1002 167 119 85 50 BsmI GAATG_Cn' Cuts at: 2323 3467 3560 2323 Size: 1144 93 4719 Fragments arranged by size: 4719 1144 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2509 2509 Size: 5956 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2118 2166 2255 2490 2634 2679 2826 Size: 399 328 48 89 235 144 45 147 Cuts at: 2826 2846 2965 3015 3251 3275 4017 4102 5173 Size: 20 119 50 236 24 742 85 1071 Cuts at: 5173 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 328 236 235 147 144 119 89 85 50 48 45 24 20 BspEI T'CCGG_A Cuts at: 5619 5619 Size: 5956 BspHI T'CATG_A Cuts at: 3845 5575 3845 Size: 1730 4226 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2378 2672 2807 3311 844 Size: 1534 294 135 504 3489 Fragments arranged by size: 3489 1534 504 294 135 BsrBI CCG'CTC Cuts at: 1394 1823 3843 1394 Size: 429 2020 3507 Fragments arranged by size: 3507 2020 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4451 4625 65 Size: 1183 3203 174 1396 Fragments arranged by size: 3203 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4600 4787 1700 Size: 110 2790 187 2869 Fragments arranged by size: 2869 2790 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5956 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5956 BssSI C'ACGA_G Cuts at: 2678 4016 5028 2678 Size: 1338 1012 3606 Fragments arranged by size: 3606 1338 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5956 BstBI TT'CG_AA Cuts at: 4760 4760 Size: 5956 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5956 BstXI CCAn_nnnn'nTGG Cuts at: 2278 2474 2278 Size: 196 5760 BstYI r'GATC_y Cuts at: 1972 3232 3286 3653 4038 4055 5507 5593 5605 Size: 1260 54 367 385 17 1452 86 12 Cuts at: 5605 5951 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1260 385 367 346 86 54 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2016 2490 2850 513 Size: 552 939 12 474 360 3619 Fragments arranged by size: 3619 939 552 474 360 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2817 487 Size: 683 56 1591 3626 Fragments arranged by size: 3626 1591 683 56 BtsI GCAGTG_nn' Cuts at: 3472 4266 4286 3472 Size: 794 20 5142 Fragments arranged by size: 5142 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5956 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2625 2660 529 Size: 35 2061 35 3825 Fragments arranged by size: 3825 2061 35 35 DraI TTT'AAA Cuts at: 3260 3260 Size: 5956 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2185 4963 809 Size: 507 869 2778 1802 Fragments arranged by size: 2778 1802 869 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2019 2030 2066 Size: 54 1425 50 275 18 189 11 36 Cuts at: 2066 2229 2776 3403 4292 5921 8 Size: 163 547 627 889 1629 43 Fragments arranged by size: 1629 1425 889 627 547 275 189 163 54 50 43 36 18 11 EagI C'GGCC_G Cuts at: 1537 1812 3403 1537 Size: 275 1591 4090 Fragments arranged by size: 4090 1591 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3229 3886 1353 Size: 1876 657 3423 Fragments arranged by size: 3423 1876 657 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4543 4917 5063 387 Size: 1064 138 2954 374 146 1280 Fragments arranged by size: 2954 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2963 2963 Size: 5956 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2496 4019 4616 5403 5671 1239 Size: 393 864 1523 597 787 268 1524 Fragments arranged by size: 1524 1523 864 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2326 2326 Size: 5956 EcoO109I rG'GnC_Cy Cuts at: 2251 2252 2855 2999 3124 2251 Size: 1 603 144 125 5083 Fragments arranged by size: 5083 603 144 125 1 EcoRI G'AATT_C Cuts at: 2282 3265 3643 2282 Size: 983 378 4595 Fragments arranged by size: 4595 983 378 EcoRV GAT'ATC Cuts at: 2413 2413 Size: 5956 FspI TGC'GCA Cuts at: 4462 4462 Size: 5956 HaeII r_GCGC'y Cuts at: 1678 2051 2105 2889 3174 5103 1678 Size: 373 54 784 285 1929 2531 Fragments arranged by size: 2531 1929 784 373 285 54 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2839 2871 4597 4629 4671 Size: 32 135 32 1267 32 1726 32 42 Cuts at: 4671 4703 1373 Size: 32 2626 Fragments arranged by size: 2626 1726 1267 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3300 3546 669 Size: 988 281 1362 246 3079 Fragments arranged by size: 3079 1362 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5956 HpaI GTT'AAC Cuts at: 3546 3546 Size: 5956 KasI G'GCGC_C Cuts at: 2101 2885 2101 Size: 784 5172 KpnI G_GTAC'C Cuts at: 2773 3281 2773 Size: 508 5448 MfeI C'AATT_G Cuts at: 3555 3555 Size: 5956 MluI A'CGCG_T Cuts at: 5832 5832 Size: 5956 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5070 5254 1305 Size: 3765 184 2007 Fragments arranged by size: 3765 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2068 2778 10 Size: 54 1425 343 236 710 3188 Fragments arranged by size: 3188 1425 710 343 236 54 MslI CAynn'nnrTG Cuts at: 518 1166 2111 2174 2849 3914 4273 4432 518 Size: 648 945 63 675 1065 359 159 2042 Fragments arranged by size: 2042 1065 945 675 648 359 159 63 MspA1I CmG'CkG Cuts at: 1606 2035 2194 2450 2499 2701 3203 3372 4049 Size: 429 159 256 49 202 502 169 677 Cuts at: 4049 5197 5442 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 502 429 256 245 202 169 159 49 NarI GG'CG_CC Cuts at: 2102 2886 2102 Size: 784 5172 NcoI C'CATG_G Cuts at: 513 1065 2004 2016 2490 2850 513 Size: 552 939 12 474 360 3619 Fragments arranged by size: 3619 939 552 474 360 12 NdeI CA'TA_TG Cuts at: 387 387 Size: 5956 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5956 NotI GC'GGCC_GC Cuts at: 3403 3403 Size: 5956 NspI r_CATG'y Cuts at: 1132 1229 2186 3025 3167 3191 3314 4859 1132 Size: 97 957 839 142 24 123 1545 2229 Fragments arranged by size: 2229 1545 957 839 142 123 97 24 PasI CC'CwG_GG Cuts at: 1348 2881 1348 Size: 1533 4423 PciI A'CATG_T Cuts at: 4855 4855 Size: 5956 PflMI CCAn_nnn'nTGG Cuts at: 1108 2619 1108 Size: 1511 4445 PmeI GTTT'AAAC Cuts at: 3260 3260 Size: 5956 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4025 4057 5538 5570 4025 Size: 32 1481 32 4411 Fragments arranged by size: 4411 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2855 2999 3124 2855 Size: 144 125 5687 Fragments arranged by size: 5687 144 125 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5956 PsiI TTA'TAA Cuts at: 3526 3526 Size: 5956 PspOMI G'GGCC_C Cuts at: 2251 2251 Size: 5956 PspXI vC'TCGA_Gb Cuts at: 3151 3151 Size: 5956 PstI C_TGCA'G Cuts at: 830 3308 830 Size: 2478 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5956 PvuII CAG'CTG Cuts at: 2194 2450 2701 3203 2194 Size: 256 251 502 4947 Fragments arranged by size: 4947 502 256 251 SacI G_AGCT'C Cuts at: 2965 2965 Size: 5956 SalI G'TCGA_C Cuts at: 1936 3298 1936 Size: 1362 4594 SanDI GG'GwC_CC Cuts at: 2855 2855 Size: 5956 SapI GCTCTTCn'nnn_ Cuts at: 3229 3229 Size: 5956 SbfI CC_TGCA'GG Cuts at: 3308 3308 Size: 5956 ScaI AGT'ACT Cuts at: 4204 5916 4204 Size: 1712 4244 SfcI C'TryA_G Cuts at: 826 1045 2156 3304 4439 5120 5311 826 Size: 219 1111 1148 1135 681 191 1471 Fragments arranged by size: 1471 1148 1135 1111 681 219 191 SfoI GGC'GCC Cuts at: 2103 2887 2103 Size: 784 5172 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5956 SmaI CCC'GGG Cuts at: 1872 3283 1872 Size: 1411 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2635 3151 4060 4961 5223 820 Size: 197 926 692 516 909 901 262 1553 Fragments arranged by size: 1553 926 909 901 692 516 262 197 SnaBI TAC'GTA Cuts at: 493 3747 493 Size: 3254 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5956 SphI G_CATG'C Cuts at: 1132 3167 3314 1132 Size: 2035 147 3774 Fragments arranged by size: 3774 2035 147 SspI AAT'ATT Cuts at: 5 52 3880 5 Size: 47 3828 2081 Fragments arranged by size: 3828 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5956 StyI C'CwwG_G Cuts at: 513 1065 2004 2016 2490 2773 2850 2956 3395 Size: 552 939 12 474 283 77 106 439 Cuts at: 3395 513 Size: 3074 Fragments arranged by size: 3074 939 552 474 439 283 106 77 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2123 3983 4000 4153 4338 4783 325 Size: 1255 543 1860 17 153 185 445 1498 Fragments arranged by size: 1860 1498 1255 543 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2724 3155 4202 Size: 274 80 33 51 1443 747 431 1047 Cuts at: 4202 5914 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1047 747 431 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2469 2501 5916 5948 2469 Size: 32 3415 32 2477 Fragments arranged by size: 3415 2477 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2090 1316 Size: 774 5182 XbaI T'CTAG_A Cuts at: 3292 3292 Size: 5956 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3043 3043 Size: 5956 XhoI C'TCGA_G Cuts at: 1943 3151 1943 Size: 1208 4748 XmaI C'CCGG_G Cuts at: 1870 3281 1870 Size: 1411 4545 XmnI GAAnn'nnTTC Cuts at: 4085 4085 Size: 5956 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2089 3764 276 Size: 53 83 186 936 555 1675 2468 Fragments arranged by size: 2468 1675 936 555 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BfrBI BsiWI BstZ17I Bsu36I DraIII FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PsrI RsrII SacII SexAI SfiI SrfI SwaI