################################## # NO CUT ENZYMES ( 191 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaII AviII AvrII BalI BanI BanII BbeI BbrPI BbsI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MslI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tsp45I Tth111I TthHB8I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AcsI 328 AAATTC [GA]^AATT[CT] AlwNI 34 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 328 AAATTC [GA]^AATT[CT] AvaI 155 CCCGGG C^[CT]CG[GA]G BamHI 66 GGATCC G^GATCC BbvCI 24 GCTGAGG CCTCAGC(-5/-2) BbvI 48 GCTGC GCAGC(8/12) BsaJI 155 CCCGGG C^C[ACGT][ACGT]GG BsmAI 30 GAGAC GTCTC(1/5) BsoBI 155 CCCGGG C^[CT]CG[GA]G BstYI 66 GGATCC [GA]^GATC[CT] CfoI 22 GCGC GCG^C EaeI 41 TGGCCG [CT]^GGCC[GA] HaeII 21 GGCGCT [GA]GCGC^[CT] HapII 156 CCGG C^CGG HhaI 22 GCGC GCG^C HinP1I 22 GCGC G^CGC HinfI 352 GACTC G^A[ACGT]TC HpaII 156 CCGG C^CGG HphI 73 GGTGA GGTGA(8/7) MaeIII 170 GTAAC ^GT[ACGT]AC MflI 66 GGATCC [GA]^GATC[CT] MspI 156 CCGG C^CGG NlaIV 66 GGATCC GG[ACGT]^[ACGT]CC PflMI 135 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 352 GACTC GAGTC(4/5) PstI 49 CTGCAG CTGCA^G SfaNI 76 GATGC GCATC(5/9) SfcI 49 CTGCAG C^T[GA][CT]AG SmaI 155 CCCGGG CCC^GGG SmlI 16 CTTGAG C^T[CT][GA]AG TseI 48 GCTGC G^C[AT]GC TspRI 323 CAGTG CA[GC]TG[ACGT][ACGT]^ Van91I 135 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 66 GGATCC [GA]^GATC[CT] XmaCI 155 CCCGGG C^CCGGG XmaI 155 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AciI 4 GCGG CCGC(-3/-1) 79 GCGG AluI 14 AGCT AG^CT 47 AGCT BcnI 155 CCCGG CC^[GC]GG 156 CCGGG BseRI 10 GAGGAG GAGGAG(10/8) 27 GAGGAG BstNI 70 CCTGG CC^[AT]GG 161 CCAGG Cac8I 39 GCTGGC GC[ACGT]^[ACGT]GC 51 GCAGGC DpnII 67 GATC ^GATC 145 GATC EcoRII 70 CCTGG ^CC[AT]GG 161 CCAGG Fnu4HI 48 GCTGC GC^[ACGT]GC 79 GCGGC ItaI 48 GCTGC GC^[ACGT]GC 79 GCGGC MboI 67 GATC ^GATC 145 GATC MboII 338 GAAGA GAAGA(8/7) 361 GAAGA MvaI 70 CCTGG CC^[AT]GG 161 CCAGG MwoI 15 GCTTGAGGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 39 GCTGGCCGAGC NciI 155 CCCGG CC^[GC]GG 156 CCGGG NdeII 67 GATC ^GATC 145 GATC Sau3AI 67 GATC ^GATC 145 GATC Tsp509I 214 AATT ^AATT 329 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 12 enzymes) # ######################################### AlwI BsiYI BslI BssKI Cfr13I DdeI HaeIII MnlI MseI Sau96I ScrFI Tru9I