(Circular) (Six-base) MAPSORT of: pFN21AB6936.seq Check: 6789 from: 1 to: 5392 pFN21AB6936 5392 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3202 278 Size: 53 83 186 936 1666 2468 Fragments arranged by size: 2468 1666 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2735 1937 Size: 798 4594 Acc65I G'GTAC_C Cuts at: 2121 2713 2121 Size: 592 4800 AclI AA'CG_TT Cuts at: 1563 3519 3892 1563 Size: 1956 373 3063 Fragments arranged by size: 3063 1956 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2513 3455 4839 5107 1632 Size: 881 942 1384 268 1917 Fragments arranged by size: 1917 1384 942 881 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5195 AflIII A'CryG_T Cuts at: 1203 4291 5268 1203 Size: 3088 977 1327 Fragments arranged by size: 3088 1327 977 AgeI A'CCGG_T Cuts at: 4223 4223 Size: 5392 AhdI GACnn_n'nnGTC Cuts at: 4121 4121 Size: 5392 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5392 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2375 1184 Size: 1191 4201 AlwNI CAG_nnn'CTG Cuts at: 3135 4707 3135 Size: 1572 3820 ApaI G_GGCC'C Cuts at: 2076 2550 2076 Size: 474 4918 ApaLI G'TGCA_C Cuts at: 3449 4605 3449 Size: 1156 4236 ApoI r'AATT_y Cuts at: 1493 2701 2927 3079 5188 1493 Size: 1208 226 152 2109 1697 Fragments arranged by size: 2109 1697 1208 226 152 AseI AT'TA_AT Cuts at: 160 1032 3946 160 Size: 872 2914 1606 Fragments arranged by size: 2914 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5392 AvaI C'yCGr_G Cuts at: 1870 1943 2097 2220 2459 2589 2717 1870 Size: 73 154 123 239 130 128 4545 Fragments arranged by size: 4545 239 154 130 128 123 73 BamHI G'GATC_C Cuts at: 2502 2722 3089 2502 Size: 220 367 4805 Fragments arranged by size: 4805 367 220 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2121 2713 4168 618 Size: 325 217 627 334 592 1455 1842 Fragments arranged by size: 1842 1455 627 592 334 325 217 BanII G_rGCy'C Cuts at: 1391 2076 2345 2550 2711 1391 Size: 685 269 205 161 4072 Fragments arranged by size: 4072 685 269 205 161 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4421 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2338 2372 3583 3617 1903 Size: 34 401 34 1211 34 3678 Fragments arranged by size: 3678 1211 401 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3284 4494 3284 Size: 1210 4182 BclI T'GATC_A Cuts at: 1548 1548 Size: 5392 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4003 136 Size: 107 122 71 631 605 2331 1525 Fragments arranged by size: 2331 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2481 5387 2481 Size: 2906 2486 BlpI GC'TnA_GC Cuts at: 2427 2809 2427 Size: 382 5010 Bme1580I G_kGCm'C Cuts at: 1790 2076 2550 3453 4609 1790 Size: 286 474 903 1156 2573 Fragments arranged by size: 2573 1156 903 474 286 BmgBI CAC'GTC Cuts at: 1318 2562 1318 Size: 1244 4148 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3123 4081 442 Size: 416 53 83 399 24 1706 958 1753 Fragments arranged by size: 1753 1706 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5392 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2503 2503 Size: 5392 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2329 2382 2674 4052 1239 Size: 1090 53 292 1378 2579 Fragments arranged by size: 2579 1378 1090 292 53 Bpu10I CC'TnA_GC Cuts at: 2414 2619 2414 Size: 205 5187 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3517 4382 4680 3517 Size: 865 298 4229 Fragments arranged by size: 4229 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4055 882 Size: 617 2556 2219 Fragments arranged by size: 2556 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2199 2311 3183 493 Size: 711 995 112 872 2702 Fragments arranged by size: 2702 995 872 711 112 BsaBI GATnn'nnATC Cuts at: 5386 5386 Size: 5392 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2252 3199 3581 4216 Size: 53 83 186 936 719 947 382 635 Cuts at: 4216 275 Size: 1451 Fragments arranged by size: 1451 947 936 719 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3825 4223 4497 4644 5055 3825 Size: 398 274 147 411 4162 Fragments arranged by size: 4162 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5167 Fragments arranged by size: 5167 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5392 BseYI C'CCAG_C Cuts at: 2077 4595 5091 2077 Size: 2518 496 2378 Fragments arranged by size: 2518 2378 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2254 2465 1163 Size: 573 518 211 4090 Fragments arranged by size: 4090 573 518 211 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2130 2842 3603 4631 1540 Size: 275 186 129 712 761 1028 2301 Fragments arranged by size: 2301 1028 761 712 275 186 129 BsiHKAI G_wGCw'C Cuts at: 2316 2379 3453 3538 4609 2316 Size: 63 1074 85 1071 3099 Fragments arranged by size: 3099 1074 1071 85 63 BsmI GAATG_Cn' Cuts at: 2903 2996 2903 Size: 93 5299 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 2316 2345 2379 2550 2711 3453 Size: 399 286 240 29 34 171 161 742 Cuts at: 3453 3538 4609 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 286 240 171 161 85 34 29 BspEI T'CCGG_A Cuts at: 5055 5055 Size: 5392 BspHI T'CATG_A Cuts at: 3281 5011 3281 Size: 1730 3662 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2747 844 Size: 1903 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3279 1394 Size: 429 1456 3507 Fragments arranged by size: 3507 1456 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2111 2190 3887 4061 65 Size: 1183 863 79 1697 174 1396 Fragments arranged by size: 1697 1396 1183 863 174 79 BsrFI r'CCGG_y Cuts at: 1700 1810 4036 4223 1700 Size: 110 2226 187 2869 Fragments arranged by size: 2869 2226 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5392 BssHII G'CGCG_C Cuts at: 1925 2528 1925 Size: 603 4789 BssSI C'ACGA_G Cuts at: 3452 4464 3452 Size: 1012 4380 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5392 BstBI TT'CG_AA Cuts at: 4196 4196 Size: 5392 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5392 BstYI r'GATC_y Cuts at: 1972 2481 2502 2722 3089 3474 3491 4943 5029 Size: 509 21 220 367 385 17 1452 86 Cuts at: 5029 5041 5387 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 509 385 367 346 220 86 21 17 12 Bsu36I CC'TnA_GG Cuts at: 2573 2573 Size: 5392 BtgI C'CryG_G Cuts at: 513 1065 2133 2298 513 Size: 552 1068 165 3607 Fragments arranged by size: 3607 1068 552 165 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4653 Fragments arranged by size: 4653 683 56 BtsI GCAGTG_nn' Cuts at: 2065 2090 2453 2667 2908 3702 3722 2065 Size: 25 363 214 241 794 20 3735 Fragments arranged by size: 3735 794 363 241 214 25 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5392 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5357 DraI TTT'AAA Cuts at: 2696 2696 Size: 5392 DraIII CAC_nnn'GTG Cuts at: 2542 2542 Size: 5392 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2257 4399 809 Size: 507 941 2142 1802 Fragments arranged by size: 2142 1802 941 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2839 3728 5357 Size: 54 1425 50 275 18 1009 889 1629 Cuts at: 5357 8 Size: 43 Fragments arranged by size: 1629 1425 1009 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2839 1537 Size: 275 1027 4090 Fragments arranged by size: 4090 1027 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3322 1353 Size: 1969 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3979 4353 4499 387 Size: 1064 138 2390 374 146 1280 Fragments arranged by size: 2390 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2329 2382 2513 2674 3455 4052 4839 Size: 393 697 53 131 161 781 597 787 Cuts at: 4839 5107 1239 Size: 268 1524 Fragments arranged by size: 1524 787 781 697 597 393 268 161 131 53 EcoNI CCTnn'n_nnAGG Cuts at: 2400 2687 2400 Size: 287 5105 EcoO109I rG'GnC_Cy Cuts at: 2072 2073 2463 2546 2623 2072 Size: 1 390 83 77 4841 Fragments arranged by size: 4841 390 83 77 1 EcoRI G'AATT_C Cuts at: 2701 3079 2701 Size: 378 5014 FspI TGC'GCA Cuts at: 3898 3898 Size: 5392 HaeII r_GCGC'y Cuts at: 1678 2434 4539 1678 Size: 756 2105 2531 Fragments arranged by size: 2531 2105 756 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4033 4065 4107 4139 1373 Size: 32 135 32 2461 32 42 32 2626 Fragments arranged by size: 2626 2461 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2736 2982 669 Size: 988 281 798 246 3079 Fragments arranged by size: 3079 988 798 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5392 HpaI GTT'AAC Cuts at: 2982 2982 Size: 5392 KpnI G_GTAC'C Cuts at: 2125 2717 2125 Size: 592 4800 MfeI C'AATT_G Cuts at: 2991 2991 Size: 5392 MluI A'CGCG_T Cuts at: 5268 5268 Size: 5392 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4506 4690 1305 Size: 3201 184 2007 Fragments arranged by size: 3201 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3570 Fragments arranged by size: 3570 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3350 3709 3868 518 Size: 648 2184 359 159 2042 Fragments arranged by size: 2184 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2046 2232 2268 2290 2808 3485 4633 4878 Size: 440 186 36 22 518 677 1148 245 Cuts at: 4878 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 518 440 245 186 36 22 NcoI C'CATG_G Cuts at: 513 1065 2298 513 Size: 552 1233 3607 Fragments arranged by size: 3607 1233 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5392 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5392 NotI GC'GGCC_GC Cuts at: 2839 2839 Size: 5392 NspI r_CATG'y Cuts at: 1132 1229 2750 4295 1132 Size: 97 1521 1545 2229 Fragments arranged by size: 2229 1545 1521 97 PasI CC'CwG_GG Cuts at: 1348 2337 1348 Size: 989 4403 PciI A'CATG_T Cuts at: 4291 4291 Size: 5392 PflMI CCAn_nnn'nTGG Cuts at: 1108 2542 1108 Size: 1434 3958 PfoI T'CCnGG_A Cuts at: 2144 2144 Size: 5392 PmeI GTTT'AAAC Cuts at: 2696 2696 Size: 5392 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3461 3493 4974 5006 3461 Size: 32 1481 32 3847 Fragments arranged by size: 3847 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2463 2623 2463 Size: 160 5232 PshAI GACnn'nnGTC Cuts at: 1613 2655 1613 Size: 1042 4350 PsiI TTA'TAA Cuts at: 2962 2962 Size: 5392 PspOMI G'GGCC_C Cuts at: 2072 2546 2072 Size: 474 4918 PstI C_TGCA'G Cuts at: 830 2060 2247 2744 830 Size: 1230 187 497 3478 Fragments arranged by size: 3478 1230 497 187 PvuI CG_AT'CG Cuts at: 2001 2130 2001 Size: 129 5263 PvuII CAG'CTG Cuts at: 2046 2232 2268 2290 2046 Size: 186 36 22 5148 Fragments arranged by size: 5148 186 36 22 SalI G'TCGA_C Cuts at: 1936 2734 1936 Size: 798 4594 SbfI CC_TGCA'GG Cuts at: 2744 2744 Size: 5392 ScaI AGT'ACT Cuts at: 2498 3640 5352 2498 Size: 1142 1712 2538 Fragments arranged by size: 2538 1712 1142 SfcI C'TryA_G Cuts at: 826 1045 2056 2243 2438 2740 3875 4556 4747 Size: 219 1011 187 195 302 1135 681 191 Cuts at: 4747 826 Size: 1471 Fragments arranged by size: 1471 1135 1011 681 302 219 195 191 187 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5392 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5392 SmaI CCC'GGG Cuts at: 1872 2099 2222 2461 2719 1872 Size: 227 123 239 258 4545 Fragments arranged by size: 4545 258 239 227 123 SmlI C'TyrA_G Cuts at: 820 1017 1943 3496 4397 4659 820 Size: 197 926 1553 901 262 1553 Fragments arranged by size: 1553 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3183 493 Size: 2690 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5392 SphI G_CATG'C Cuts at: 1132 2750 1132 Size: 1618 3774 SspI AAT'ATT Cuts at: 5 52 3316 5 Size: 47 3264 2081 Fragments arranged by size: 3264 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5392 StyI C'CwwG_G Cuts at: 513 1065 2298 2831 513 Size: 552 1233 533 3074 Fragments arranged by size: 3074 1233 552 533 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3419 3436 3589 3774 4219 325 Size: 1255 1839 17 153 185 445 1498 Fragments arranged by size: 1839 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2496 3638 5350 Size: 274 80 33 51 1443 519 1142 1712 Cuts at: 5350 96 Size: 138 Fragments arranged by size: 1712 1443 1142 519 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5352 5384 5352 Size: 32 5360 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5392 XbaI T'CTAG_A Cuts at: 2728 2728 Size: 5392 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2680 2680 Size: 5392 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5392 XmaI C'CCGG_G Cuts at: 1870 2097 2220 2459 2717 1870 Size: 227 123 239 258 4545 Fragments arranged by size: 4545 258 239 227 123 XmnI GAAnn'nnTTC Cuts at: 3521 3521 Size: 5392 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3200 276 Size: 53 83 186 936 1666 2468 Fragments arranged by size: 2468 1666 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI