(Linear) (Six-base) MAPSORT of: KIEE9658.seq Check: 3905 from: 1 to: 633 KIEE9658.orf With 182 enzymes: SgfI * January 29, 2014 12:07 .. AatII G_ACGT'C Cuts at: 0 580 633 Size: 580 53 AccI GT'mk_AC Cuts at: 0 107 633 Size: 107 526 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 403 633 Size: 403 230 ApaLI G'TGCA_C Cuts at: 0 448 633 Size: 448 185 ApoI r'AATT_y Cuts at: 0 289 633 Size: 289 344 BamHI G'GATC_C Cuts at: 0 512 633 Size: 512 121 BfrBI ATG'CAT Cuts at: 0 543 633 Size: 543 90 Bme1580I G_kGCm'C Cuts at: 0 452 633 Size: 452 181 BmrI ACTGGGnnnn_n' Cuts at: 0 274 633 Size: 274 359 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 88 244 633 Size: 88 156 389 Fragments arranged by size: 389 156 88 BsaAI yAC'GTr Cuts at: 0 96 606 633 Size: 96 510 27 Fragments arranged by size: 510 96 27 BsaHI Gr'CG_yC Cuts at: 0 577 633 Size: 577 56 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 297 327 633 Size: 297 30 306 Fragments arranged by size: 306 297 30 BseYI C'CCAG_C Cuts at: 0 308 633 Size: 308 325 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 187 633 Size: 187 446 BsiHKAI G_wGCw'C Cuts at: 0 56 275 452 633 Size: 56 219 177 181 Fragments arranged by size: 219 181 177 56 Bsp1286I G_dGCh'C Cuts at: 0 56 275 452 633 Size: 56 219 177 181 Fragments arranged by size: 219 181 177 56 BsrFI r'CCGG_y Cuts at: 0 150 633 Size: 150 483 BssHII G'CGCG_C Cuts at: 0 615 633 Size: 615 18 BssSI C'ACGA_G Cuts at: 0 453 633 Size: 453 180 BstYI r'GATC_y Cuts at: 0 485 512 633 Size: 485 27 121 Fragments arranged by size: 485 121 27 BtgI C'CryG_G Cuts at: 0 113 633 Size: 113 520 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 413 633 Size: 413 220 BtsI GCAGTG_nn' Cuts at: 0 204 316 633 Size: 204 112 317 Fragments arranged by size: 317 204 112 DraI TTT'AAA Cuts at: 0 590 633 Size: 590 43 EaeI y'GGCC_r Cuts at: 0 33 44 633 Size: 33 11 589 Fragments arranged by size: 589 33 11 EarI CTCTTCn'nnn_ Cuts at: 0 605 633 Size: 605 28 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 535 633 Size: 535 98 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 88 244 403 633 Size: 88 156 159 230 Fragments arranged by size: 230 159 156 88 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 8 40 633 Size: 8 32 593 Fragments arranged by size: 593 32 8 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 560 633 Size: 560 73 MscI TGG'CCA Cuts at: 0 35 633 Size: 35 598 MslI CAynn'nnrTG Cuts at: 0 542 633 Size: 542 91 MspA1I CmG'CkG Cuts at: 0 363 633 Size: 363 270 NsiI A_TGCA'T Cuts at: 0 545 633 Size: 545 88 NspI r_CATG'y Cuts at: 0 543 633 Size: 543 90 PflMI CCAn_nnn'nTGG Cuts at: 0 42 633 Size: 42 591 PfoI T'CCnGG_A Cuts at: 0 65 191 221 407 633 Size: 65 126 30 186 226 Fragments arranged by size: 226 186 126 65 30 PvuII CAG'CTG Cuts at: 0 363 633 Size: 363 270 SapI GCTCTTCn'nnn_ Cuts at: 0 605 633 Size: 605 28 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 322 633 Size: 322 311 TatI w'GTAC_w Cuts at: 0 140 633 Size: 140 493 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 297 329 633 Size: 297 32 304 Fragments arranged by size: 304 297 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 474 633 Size: 474 159 ZraI GAC'GTC Cuts at: 0 578 633 Size: 578 55 Enzymes that do cut: AatII AccI AcuI ApaLI ApoI BamHI BfrBI Bme1580I BmrI BpmI BsaAI BsaHI BsaXI BseYI BsgI BsiHKAI Bsp1286I BsrFI BssHII BssSI BstYI BtgI BtgZI BtsI DraI EaeI EarI EciI Eco57MI Hin4I MmeI MscI MslI MspA1I NsiI NspI PflMI PfoI PvuII SapI TaqII TatI TstI XcmI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI AscI AseI AsiSI AvaI AvrII BaeI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaWI BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII DrdI EagI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI Tth111I XbaI XhoI XmaI XmnI