################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI AlwI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BfrI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspMI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DrdI Eam1105I Eco47III Eco81I EcoNI EcoRI EcoRV EcoT22I FseI HgaI Hin1I HindIII KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccII 24 CGCG CG^CG AflIII 991 ACATGT A^C[GA][CT]GT Alw44I 516 GTGCAC G^TGCAC ApaLI 516 GTGCAC G^TGCAC Asp700I 972 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 586 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 852 CTCGGG C^[CT]CG[GA]G AviII 299 TGCGCA TGC^GCA BanII 1317 GAGCCC G[GA]GC[CT]^C BclI 99 TGATCA T^GATCA BfaI 949 CTAG C^TAG BglII 754 AGATCT A^GATCT BsgI 645 CTGCAC GTGCAG(16/14) BsiEI 53 CGGCCG CG[GA][CT]^CG BsmI 614 GAATGC GAATGC(1/-1) BsoBI 852 CTCGGG C^[CT]CG[GA]G BspHI 872 TCATGA T^CATGA BspLU11I 991 ACATGT A^CATGT BsrDI 1022 GCAATG GCAATG(2/0) BstEII 1338 GGTAACC G^GT[ACGT]ACC BstPI 1338 GGTAACC G^GT[ACGT]ACC BstUI 24 CGCG CG^CG BstYI 754 AGATCT [GA]^GATC[CT] EagI 53 CGGCCG C^GGCCG EclXI 53 CGGCCG C^GGCCG Eco52I 53 CGGCCG C^GGCCG EcoO65I 1338 GGTAACC G^GT[ACGT]ACC FbaI 99 TGATCA T^GATCA FspI 299 TGCGCA TGC^GCA HaeII 220 AGCGCC [GA]GCGC^[CT] HincII 370 GTTAAC GT[CT]^[GA]AC HindII 370 GTTAAC GT[CT]^[GA]AC HpaI 370 GTTAAC GTT^AAC HphI 509 TCACC GGTGA(8/7) MaeI 949 CTAG C^TAG MflI 754 AGATCT [GA]^GATC[CT] MvnI 24 CGCG CG^CG NdeI 359 CATATG CA^TATG PflFI 586 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1303 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 590 AGGTCCC [GA]G^G[AT]CC[CT] PvuII 810 CAGCTG CAG^CTG RcaI 872 TCATGA T^CATGA SapI 618 GCTCTTC GCTCTTC(1/4) SbfI 327 CCTGCAGG CCTGCA^GG Sse8387I 327 CCTGCAGG CCTGCA^GG StuI 461 AGGCCT AGG^CCT TaqI 473 TCGA T^CGA TfiI 352 GATTC G^A[AT]TC Tth111I 586 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 473 TCGA T^CGA Van91I 1303 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 754 AGATCT [GA]^GATC[CT] XmnI 972 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### BglI 223 GCCATGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1113 GCCCCTGGGGC BseRI 841 CTCCTC GAGGAG(10/8) 1298 CTCCTC DraIII 510 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG 1163 CACCGTGTG DsaI 224 CCATGG C^C[GA][CT]GG 916 CCATGG EaeI 53 CGGCCG [CT]^GGCC[GA] 596 CGGCCA FauI 160 GCGGG CCCGC(4/6) 270 GCGGG MspA1I 261 CCGCTG C[AC]G^C[GT]G 810 CAGCTG NcoI 224 CCATGG C^CATGG 916 CCATGG NspI 314 GCATGT [GA]CATG^[CT] 991 ACATGT SfaNI 451 GATGC GCATC(5/9) 187 GCATC Tsp45I 923 GTGAC ^GT[GC]AC 1036 GTGAC XcmI 173 CCAACTTCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1280 CCACAAAATCAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AciI AcsI AfaI AluI AlwNI ApoI AspHI AvaII BbvI BcnI BmyI BpmI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrBI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI Ksp632I MaeIII MboI MboII MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfcI StyI Tru9I TseI Tsp509I TspRI