(Circular) (Six-base) MAPSORT of: pFN21AE1556.seq Check: 4619 from: 1 to: 6085 pFN21AE1556 6085 bp With 182 enzymes: SgfI * August 16, 2010 14:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3895 278 Size: 53 83 186 936 2359 2468 Fragments arranged by size: 2468 2359 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3428 1937 Size: 1491 4594 Acc65I G'GTAC_C Cuts at: 3406 3406 Size: 6085 AclI AA'CG_TT Cuts at: 1563 4212 4585 1563 Size: 2649 373 3063 Fragments arranged by size: 3063 2649 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2135 2167 2261 4148 5532 5800 1632 Size: 503 32 94 1887 1384 268 1917 Fragments arranged by size: 1917 1887 1384 503 268 94 32 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5888 AflIII A'CryG_T Cuts at: 1203 2996 4984 5961 1203 Size: 1793 1988 977 1327 Fragments arranged by size: 1988 1793 1327 977 AgeI A'CCGG_T Cuts at: 4916 4916 Size: 6085 AhdI GACnn_n'nnGTC Cuts at: 4814 4814 Size: 6085 AleI CACnn'nnGTG Cuts at: 1166 2531 1166 Size: 1365 4720 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6085 AlwNI CAG_nnn'CTG Cuts at: 2113 2332 2817 3137 3196 3828 5400 2113 Size: 219 485 320 59 632 1572 2798 Fragments arranged by size: 2798 1572 632 485 320 219 59 ApaLI G'TGCA_C Cuts at: 2521 4142 5298 2521 Size: 1621 1156 3308 Fragments arranged by size: 3308 1621 1156 ApoI r'AATT_y Cuts at: 1493 2722 2969 2977 3147 3178 3394 3620 3772 Size: 1229 247 8 170 31 216 226 152 Cuts at: 3772 5881 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1229 247 226 216 170 152 31 8 AseI AT'TA_AT Cuts at: 160 1032 4639 160 Size: 872 3607 1606 Fragments arranged by size: 3607 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6085 AvaI C'yCGr_G Cuts at: 1870 1943 2857 3410 1870 Size: 73 914 553 4545 Fragments arranged by size: 4545 914 553 73 BamHI G'GATC_C Cuts at: 3415 3782 3415 Size: 367 5718 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3406 4861 618 Size: 325 217 627 1619 1455 1842 Fragments arranged by size: 1842 1619 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3326 3404 1391 Size: 1935 78 4072 Fragments arranged by size: 4072 1935 78 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5114 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4276 4310 1903 Size: 34 2339 34 3678 Fragments arranged by size: 3678 2339 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3977 5187 3977 Size: 1210 4875 BclI T'GATC_A Cuts at: 1548 2104 1548 Size: 556 5529 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2234 3124 4696 Size: 107 122 71 631 605 562 890 1572 Cuts at: 4696 136 Size: 1525 Fragments arranged by size: 1572 1525 890 631 605 562 122 107 71 BglII A'GATC_T Cuts at: 2759 6080 2759 Size: 3321 2764 BlpI GC'TnA_GC Cuts at: 3502 3502 Size: 6085 Bme1580I G_kGCm'C Cuts at: 1790 2525 4146 5302 1790 Size: 735 1621 1156 2573 Fragments arranged by size: 2573 1621 1156 735 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6085 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3059 3816 4774 Size: 416 53 83 399 24 1642 757 958 Cuts at: 4774 442 Size: 1753 Fragments arranged by size: 1753 1642 958 757 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6085 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2222 2335 2372 2884 4745 1239 Size: 983 113 37 512 1861 2579 Fragments arranged by size: 2579 1861 983 512 113 37 Bpu10I CC'TnA_GC Cuts at: 2150 2150 Size: 6085 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4210 5075 5373 4210 Size: 865 298 4922 Fragments arranged by size: 4922 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2070 2168 3266 4748 882 Size: 617 571 98 1098 1482 2219 Fragments arranged by size: 2219 1482 1098 617 571 98 BsaAI yAC'GTr Cuts at: 493 1204 3876 493 Size: 711 2672 2702 Fragments arranged by size: 2702 2672 711 BsaBI GATnn'nnATC Cuts at: 6079 6079 Size: 6085 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3892 4274 4909 275 Size: 53 83 186 936 2359 382 635 1451 Fragments arranged by size: 2359 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4518 4916 5190 5337 5748 4518 Size: 398 274 147 411 4855 Fragments arranged by size: 4855 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2882 2912 1178 Size: 30 165 30 1479 30 4351 Fragments arranged by size: 4351 1479 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2837 3294 1186 Size: 1651 457 3977 Fragments arranged by size: 3977 1651 457 BseYI C'CCAG_C Cuts at: 5288 5784 5288 Size: 496 5589 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2635 1163 Size: 573 899 4613 Fragments arranged by size: 4613 899 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2061 3535 4296 5324 1540 Size: 275 186 60 1474 761 1028 2301 Fragments arranged by size: 2301 1474 1028 761 275 186 60 BsiHKAI G_wGCw'C Cuts at: 2430 2525 2690 4146 4231 5302 2430 Size: 95 165 1456 85 1071 3213 Fragments arranged by size: 3213 1456 1071 165 95 85 BsmI GAATG_Cn' Cuts at: 2625 3596 3689 2625 Size: 971 93 5021 Fragments arranged by size: 5021 971 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2430 2525 2690 3326 3404 4146 4231 Size: 399 640 95 165 636 78 742 85 Cuts at: 4231 5302 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 640 636 399 165 95 85 78 BspEI T'CCGG_A Cuts at: 5748 5748 Size: 6085 BspHI T'CATG_A Cuts at: 2877 3974 5704 2877 Size: 1097 1730 3258 Fragments arranged by size: 3258 1730 1097 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3440 844 Size: 2596 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2038 2070 2659 3350 3972 1394 Size: 429 215 32 589 691 622 3507 Fragments arranged by size: 3507 691 622 589 429 215 32 BsrDI GCAATG_nn' Cuts at: 65 1248 3034 4580 4754 65 Size: 1183 1786 1546 174 1396 Fragments arranged by size: 1786 1546 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4729 4916 1700 Size: 110 2919 187 2869 Fragments arranged by size: 2919 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6085 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6085 BssSI C'ACGA_G Cuts at: 4145 5157 4145 Size: 1012 5073 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6085 BstBI TT'CG_AA Cuts at: 4889 4889 Size: 6085 BstEII G'GTnAC_C Cuts at: 1184 3343 1184 Size: 2159 3926 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6085 BstYI r'GATC_y Cuts at: 1972 2759 3415 3782 4167 4184 5636 5722 5734 Size: 787 656 367 385 17 1452 86 12 Cuts at: 5734 6080 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 787 656 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2229 2921 513 Size: 552 1164 692 3677 Fragments arranged by size: 3677 1164 692 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2629 487 Size: 683 56 1403 3943 Fragments arranged by size: 3943 1403 683 56 BtsI GCAGTG_nn' Cuts at: 2162 3078 3093 3326 3601 4395 4415 2162 Size: 916 15 233 275 794 20 3832 Fragments arranged by size: 3832 916 794 275 233 20 15 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6085 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6050 DraI TTT'AAA Cuts at: 3389 3389 Size: 6085 DraIII CAC_nnn'GTG Cuts at: 2520 3173 2520 Size: 653 5432 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5092 809 Size: 507 3776 1802 Fragments arranged by size: 3776 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2058 2601 3532 Size: 54 1425 50 275 18 228 543 931 Cuts at: 3532 4421 6050 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 931 889 543 275 228 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2058 3532 1537 Size: 275 246 1474 4090 Fragments arranged by size: 4090 1474 275 246 EarI CTCTTCn'nnn_ Cuts at: 1353 2630 2691 2952 4015 1353 Size: 1277 61 261 1063 3423 Fragments arranged by size: 3423 1277 1063 261 61 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2088 4672 5046 5192 387 Size: 1064 138 499 2584 374 146 1280 Fragments arranged by size: 2584 1280 1064 499 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2135 2167 2222 2261 2335 2372 2884 Size: 393 503 32 55 39 74 37 512 Cuts at: 2884 4148 4745 5532 5800 1239 Size: 1264 597 787 268 1524 Fragments arranged by size: 1524 1264 787 597 512 503 393 268 74 55 39 37 32 EcoO109I rG'GnC_Cy Cuts at: 2236 2572 2596 2236 Size: 336 24 5725 Fragments arranged by size: 5725 336 24 EcoRI G'AATT_C Cuts at: 3394 3772 3394 Size: 378 5707 FspI TGC'GCA Cuts at: 2306 4591 2306 Size: 2285 3800 HaeII r_GCGC'y Cuts at: 1678 2229 5232 1678 Size: 551 3003 2531 Fragments arranged by size: 3003 2531 551 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2658 2690 2789 2821 4726 Size: 32 135 32 1086 32 99 32 1905 Cuts at: 4726 4758 4800 4832 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1905 1086 135 99 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2377 3429 3675 669 Size: 988 281 439 1052 246 3079 Fragments arranged by size: 3079 1052 988 439 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6085 HpaI GTT'AAC Cuts at: 2377 3675 2377 Size: 1298 4787 KpnI G_GTAC'C Cuts at: 3410 3410 Size: 6085 MfeI C'AATT_G Cuts at: 3684 3684 Size: 6085 MluI A'CGCG_T Cuts at: 5961 5961 Size: 6085 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5199 5383 1305 Size: 3894 184 2007 Fragments arranged by size: 3894 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4263 Fragments arranged by size: 4263 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2420 2531 2876 4043 4402 4561 518 Size: 648 1254 111 345 1167 359 159 2042 Fragments arranged by size: 2042 1254 1167 648 359 345 159 111 MspA1I CmG'CkG Cuts at: 1606 2268 2817 3501 4178 5326 5571 1606 Size: 662 549 684 677 1148 245 2120 Fragments arranged by size: 2120 1148 684 677 662 549 245 NcoI C'CATG_G Cuts at: 513 1065 2229 2921 513 Size: 552 1164 692 3677 Fragments arranged by size: 3677 1164 692 552 NdeI CA'TA_TG Cuts at: 387 2365 387 Size: 1978 4107 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6085 NotI GC'GGCC_GC Cuts at: 3532 3532 Size: 6085 NspI r_CATG'y Cuts at: 1132 1229 2323 3000 3443 4988 1132 Size: 97 1094 677 443 1545 2229 Fragments arranged by size: 2229 1545 1094 677 443 97 PasI CC'CwG_GG Cuts at: 1348 3121 1348 Size: 1773 4312 PciI A'CATG_T Cuts at: 2996 4984 2996 Size: 1988 4097 PflMI CCAn_nnn'nTGG Cuts at: 1108 3314 1108 Size: 2206 3879 PmeI GTTT'AAAC Cuts at: 3389 3389 Size: 6085 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4154 4186 5667 5699 4154 Size: 32 1481 32 4540 Fragments arranged by size: 4540 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2596 2596 Size: 6085 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6085 PsiI TTA'TAA Cuts at: 3655 3655 Size: 6085 PstI C_TGCA'G Cuts at: 830 2132 2337 2759 2936 3437 830 Size: 1302 205 422 177 501 3478 Fragments arranged by size: 3478 1302 501 422 205 177 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6085 PvuII CAG'CTG Cuts at: 2817 2817 Size: 6085 SalI G'TCGA_C Cuts at: 1936 3427 1936 Size: 1491 4594 SapI GCTCTTCn'nnn_ Cuts at: 2630 2630 Size: 6085 SbfI CC_TGCA'GG Cuts at: 2337 3437 2337 Size: 1100 4985 ScaI AGT'ACT Cuts at: 4333 6045 4333 Size: 1712 4373 SfcI C'TryA_G Cuts at: 826 1045 2128 2333 2755 2932 3433 4568 5249 Size: 219 1083 205 422 177 501 1135 681 Cuts at: 5249 5440 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1083 681 501 422 219 205 191 177 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6085 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6085 SmaI CCC'GGG Cuts at: 1872 3412 1872 Size: 1540 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4189 5090 5352 820 Size: 197 926 2246 901 262 1553 Fragments arranged by size: 2246 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3876 493 Size: 3383 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6085 SphI G_CATG'C Cuts at: 1132 3443 1132 Size: 2311 3774 SspI AAT'ATT Cuts at: 5 52 4009 5 Size: 47 3957 2081 Fragments arranged by size: 3957 2081 47 StuI AGG'CCT Cuts at: 1517 2468 1517 Size: 951 5134 StyI C'CwwG_G Cuts at: 513 1065 2208 2229 2921 3524 513 Size: 552 1143 21 692 603 3074 Fragments arranged by size: 3074 1143 692 603 552 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4112 4129 4282 4467 4912 325 Size: 1255 2532 17 153 185 445 1498 Fragments arranged by size: 2532 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2022 2496 3067 Size: 274 80 33 51 1443 45 474 571 Cuts at: 3067 4331 6043 96 Size: 1264 1712 138 Fragments arranged by size: 1712 1443 1264 571 474 274 138 80 51 45 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3318 3350 6045 6077 3318 Size: 32 2695 32 3326 Fragments arranged by size: 3326 2695 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2594 1316 Size: 1278 4807 XbaI T'CTAG_A Cuts at: 3421 3421 Size: 6085 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2185 3292 2185 Size: 1107 4978 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6085 XmaI C'CCGG_G Cuts at: 1870 3410 1870 Size: 1540 4545 XmnI GAAnn'nnTTC Cuts at: 2981 4214 2981 Size: 1233 4852 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3893 276 Size: 53 83 186 936 2359 2468 Fragments arranged by size: 2468 2359 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI