################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BclI BfrI BglI BlnI BsaAI BsaBI BseAI BsgI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FbaI FseI FspI HgaI HindIII KasI KpnI KspI MamI MfeI MroI MunI NaeI NarI NgoMIV NotI NruI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AatII 1138 GACGTC GACGT^C AccI 970 GTCTAC GT^[AC][GT]AC AcyI 1138 GACGTC G[GA]^CG[CT]C AflIII 1412 ACGCGT A^C[GA][CT]GT Asp700I 313 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 1747 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1133 CCCGGG C^[CT]CG[GA]G AvaII 1975 GGACC G^G[AT]CC BanI 145 GGTGCC G^G[CT][GA]CC BbvCI 1641 GCTGAGG CCTCAGC(-5/-2) BlpI 1761 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1761 GCTGAGC GC^T[ACGT]AGC BsaHI 1138 GACGTC G[GA]^CG[CT]C BsaWI 1169 ACCGGA [AT]^CCGG[AT] BsoBI 1133 CCCGGG C^[CT]CG[GA]G BspMI 1232 ACCTGC ACCTGC(4/8) BsrFI 1517 ACCGGC [GA]^CCGG[CT] BstXI 2120 CCATGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1761 GCTGAGC GC^T[ACGT]AGC Cfr10I 1517 ACCGGC [GA]^CCGG[CT] DraI 1725 TTTAAA TTT^AAA Hin1I 1138 GACGTC G[GA]^CG[CT]C HpaI 1315 GTTAAC GTT^AAC HphI 1696 TCACC GGTGA(8/7) MaeII 1139 ACGT A^CGT MluI 1412 ACGCGT A^CGCGT NdeI 1037 CATATG CA^TATG NheI 2140 GCTAGC G^CTAGC NspI 338 GCATGT [GA]CATG^[CT] PflFI 1747 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC SmaI 1133 CCCGGG CCC^GGG Tsp45I 1195 GTGAC ^GT[GC]AC Tth111I 1747 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 1133 CCCGGG C^CCGGG XmaI 1133 CCCGGG C^CCGGG XmnI 313 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AfaI 871 GTAC GT^AC 997 GTAC ApaI 1583 GGGCCC GGGCC^C 1777 GGGCCC BglII 1208 AGATCT A^GATCT 1473 AGATCT BsrBI 327 CCGCTC CCGCTC(-3/-3) 1404 CCGCTC BstAPI 1278 GCAACAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1355 GCAGCGCCTGC BstBI 320 TTCGAA TT^CGAA 957 TTCGAA DraII 1582 AGGGCCC [GA]G^G[ACGT]CC[CT] 1583 GGGCCCT DsaI 5 CCATGG C^C[GA][CT]GG 1124 CCATGG EcoO109I 1582 AGGGCCC [GA]G^G[ACGT]CC[CT] 1583 GGGCCCT EcoT22I 1103 ATGCAT ATGCA^T 1450 ATGCAT HaeII 1357 AGCGCC [GA]GCGC^[CT] 1839 GGCGCT HincII 286 GTCAAC GT[CT]^[GA]AC 1315 GTTAAC HindII 286 GTCAAC GT[CT]^[GA]AC 1315 GTTAAC MaeIII 256 GTAAC ^GT[ACGT]AC 1195 GTGAC MspA1I 404 CAGCTG C[AC]G^C[GT]G 2125 CAGCTG NcoI 5 CCATGG C^CATGG 1124 CCATGG NsiI 1103 ATGCAT ATGCA^T 1450 ATGCAT NspV 320 TTCGAA TT^CGAA 957 TTCGAA PvuII 404 CAGCTG CAG^CTG 2125 CAGCTG RsaI 871 GTAC GT^AC 997 GTAC SfuI 320 TTCGAA TT^CGAA 957 TTCGAA SmlI 1954 CTCAAG C^T[CT][GA]AG 1987 CTTGAG StuI 451 AGGCCT AGG^CCT 2105 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AccII AciI AcsI AluI AlwI AlwNI ApoI AspHI BalI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI Ksp632I MaeI MboI MboII MflI MluNI MnlI MscI MseI MslI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PstI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XcmI XhoII