(Linear) (Six-base) MAPSORT of: KIEE0500.seq Check: 5348 from: 1 to: 2175 KIEE0500.orf With 182 enzymes: SgfI * February 17, 2010 19:07 .. AatII G_ACGT'C Cuts at: 0 1142 2175 Size: 1142 1033 AccI GT'mk_AC Cuts at: 0 971 2175 Size: 971 1204 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 4 1171 1588 2175 Size: 4 1167 417 587 Fragments arranged by size: 1167 587 417 4 AflIII A'CryG_T Cuts at: 0 1412 2175 Size: 1412 763 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1400 2175 Size: 1400 775 AlwNI CAG_nnn'CTG Cuts at: 0 472 1361 1641 2175 Size: 472 889 280 534 Fragments arranged by size: 889 534 472 280 ApaI G_GGCC'C Cuts at: 0 1587 1781 2175 Size: 1587 194 394 Fragments arranged by size: 1587 394 194 ApoI r'AATT_y Cuts at: 0 296 691 712 2175 Size: 296 395 21 1463 Fragments arranged by size: 1463 395 296 21 AvaI C'yCGr_G Cuts at: 0 1133 2175 Size: 1133 1042 BanI G'GyrC_C Cuts at: 0 145 2175 Size: 145 2030 BanII G_rGCy'C Cuts at: 0 660 1587 1768 1781 1955 1994 2175 Size: 660 927 181 13 174 39 181 Fragments arranged by size: 927 660 181 181 174 39 13 BbsI GAAGACnn'nnnn_ Cuts at: 0 310 1134 1874 2175 Size: 310 824 740 301 Fragments arranged by size: 824 740 310 301 BbvCI CC'TCA_GC Cuts at: 0 1642 2175 Size: 1642 533 BfrBI ATG'CAT Cuts at: 0 1105 1452 2175 Size: 1105 347 723 Fragments arranged by size: 1105 723 347 BglII A'GATC_T Cuts at: 0 1208 1473 2175 Size: 1208 265 702 Fragments arranged by size: 1208 702 265 BlpI GC'TnA_GC Cuts at: 0 1762 2175 Size: 1762 413 Bme1580I G_kGCm'C Cuts at: 0 1587 1781 2175 Size: 1587 194 394 Fragments arranged by size: 1587 394 194 BmrI ACTGGGnnnn_n' Cuts at: 0 696 1775 2175 Size: 696 1079 400 Fragments arranged by size: 1079 696 400 BmtI G_CTAG'C Cuts at: 0 2144 2175 Size: 2144 31 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 931 1285 1951 2047 2175 Size: 931 354 666 96 128 Fragments arranged by size: 931 666 354 128 96 Bpu10I CC'TnA_GC Cuts at: 0 371 659 1642 2175 Size: 371 288 983 533 Fragments arranged by size: 983 533 371 288 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1939 2008 2175 Size: 1939 69 167 Fragments arranged by size: 1939 167 69 BsaI GGTCTCn'nnnn_ Cuts at: 0 46 1073 2023 2175 Size: 46 1027 950 152 Fragments arranged by size: 1027 950 152 46 BsaHI Gr'CG_yC Cuts at: 0 1139 2175 Size: 1139 1036 BsaWI w'CCGG_w Cuts at: 0 1169 2175 Size: 1169 1006 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1864 1894 2175 Size: 1864 30 281 Fragments arranged by size: 1864 281 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 514 853 1885 1948 2175 Size: 514 339 1032 63 227 Fragments arranged by size: 1032 514 339 227 63 BseYI C'CCAG_C Cuts at: 0 2063 2175 Size: 2063 112 BsiHKAI G_wGCw'C Cuts at: 0 660 770 1955 1994 2175 Size: 660 110 1185 39 181 Fragments arranged by size: 1185 660 181 110 39 BsmI GAATG_Cn' Cuts at: 0 922 1107 1454 2175 Size: 922 185 347 721 Fragments arranged by size: 922 721 347 185 Bsp1286I G_dGCh'C Cuts at: 0 660 770 1587 1768 1781 1955 1994 2175 Size: 660 110 817 181 13 174 39 181 Fragments arranged by size: 817 660 181 181 174 110 39 13 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1241 2175 Size: 1241 934 BsrBI CCG'CTC Cuts at: 0 329 1406 2175 Size: 329 1077 769 Fragments arranged by size: 1077 769 329 BsrFI r'CCGG_y Cuts at: 0 1517 2175 Size: 1517 658 BstAPI GCAn_nnn'nTGC Cuts at: 0 1284 1361 2175 Size: 1284 77 814 Fragments arranged by size: 1284 814 77 BstBI TT'CG_AA Cuts at: 0 321 958 2175 Size: 321 637 1217 Fragments arranged by size: 1217 637 321 BstXI CCAn_nnnn'nTGG Cuts at: 0 2127 2175 Size: 2127 48 BstYI r'GATC_y Cuts at: 0 53 280 1115 1208 1473 2175 Size: 53 227 835 93 265 702 Fragments arranged by size: 835 702 265 227 93 53 BtgI C'CryG_G Cuts at: 0 5 1124 2175 Size: 5 1119 1051 Fragments arranged by size: 1119 1051 5 BtsI GCAGTG_nn' Cuts at: 0 473 2175 Size: 473 1702 DraI TTT'AAA Cuts at: 0 1727 2175 Size: 1727 448 EaeI y'GGCC_r Cuts at: 0 2 818 1041 2117 2175 Size: 2 816 223 1076 58 Fragments arranged by size: 1076 816 223 58 2 EarI CTCTTCn'nnn_ Cuts at: 0 503 543 611 646 758 1808 1862 1943 Size: 503 40 68 35 112 1050 54 81 Cuts at: 1943 2126 2175 Size: 183 49 Fragments arranged by size: 1050 503 183 112 81 68 54 49 40 35 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1091 2175 Size: 1091 1084 EcoICRI GAG'CTC Cuts at: 0 658 1953 1992 2175 Size: 658 1295 39 183 Fragments arranged by size: 1295 658 183 39 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 4 931 1171 1285 1588 1951 2047 2175 Size: 4 927 240 114 303 363 96 128 Fragments arranged by size: 927 363 303 240 128 114 96 4 EcoO109I rG'GnC_Cy Cuts at: 0 1583 1584 2175 Size: 1583 1 591 Fragments arranged by size: 1583 591 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 334 1575 2175 Size: 334 1241 600 Fragments arranged by size: 1241 600 334 HaeII r_GCGC'y Cuts at: 0 1361 1843 2175 Size: 1361 482 332 Fragments arranged by size: 1361 482 332 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1864 1896 2084 2116 2175 Size: 1864 32 188 32 59 Fragments arranged by size: 1864 188 59 32 32 HincII GTy'rAC Cuts at: 0 288 1317 2175 Size: 288 1029 858 Fragments arranged by size: 1029 858 288 HpaI GTT'AAC Cuts at: 0 1317 2175 Size: 1317 858 MluI A'CGCG_T Cuts at: 0 1412 2175 Size: 1412 763 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 81 2175 Size: 81 2094 MscI TGG'CCA Cuts at: 0 4 820 1043 2119 2175 Size: 4 816 223 1076 56 Fragments arranged by size: 1076 816 223 56 4 MslI CAynn'nnrTG Cuts at: 0 337 388 1339 2175 Size: 337 51 951 836 Fragments arranged by size: 951 836 337 51 MspA1I CmG'CkG Cuts at: 0 406 2127 2175 Size: 406 1721 48 Fragments arranged by size: 1721 406 48 NcoI C'CATG_G Cuts at: 0 5 1124 2175 Size: 5 1119 1051 Fragments arranged by size: 1119 1051 5 NdeI CA'TA_TG Cuts at: 0 1038 2175 Size: 1038 1137 NheI G'CTAG_C Cuts at: 0 2140 2175 Size: 2140 35 NsiI A_TGCA'T Cuts at: 0 1107 1454 2175 Size: 1107 347 721 Fragments arranged by size: 1107 721 347 NspI r_CATG'y Cuts at: 0 342 2175 Size: 342 1833 PasI CC'CwG_GG Cuts at: 0 1587 2175 Size: 1587 588 PspOMI G'GGCC_C Cuts at: 0 1583 1777 2175 Size: 1583 194 398 Fragments arranged by size: 1583 398 194 PstI C_TGCA'G Cuts at: 0 405 468 1304 1366 2175 Size: 405 63 836 62 809 Fragments arranged by size: 836 809 405 63 62 PvuII CAG'CTG Cuts at: 0 406 2127 2175 Size: 406 1721 48 Fragments arranged by size: 1721 406 48 SacI G_AGCT'C Cuts at: 0 660 1955 1994 2175 Size: 660 1295 39 181 Fragments arranged by size: 1295 660 181 39 SapI GCTCTTCn'nnn_ Cuts at: 0 503 543 758 1943 2126 2175 Size: 503 40 215 1185 183 49 Fragments arranged by size: 1185 503 215 183 49 40 SfcI C'TryA_G Cuts at: 0 401 464 972 1300 1362 2175 Size: 401 63 508 328 62 813 Fragments arranged by size: 813 508 401 328 63 62 SmaI CCC'GGG Cuts at: 0 1135 2175 Size: 1135 1040 SmlI C'TyrA_G Cuts at: 0 1954 1987 2175 Size: 1954 33 188 Fragments arranged by size: 1954 188 33 StuI AGG'CCT Cuts at: 0 453 2107 2175 Size: 453 1654 68 Fragments arranged by size: 1654 453 68 StyI C'CwwG_G Cuts at: 0 5 1124 1862 2175 Size: 5 1119 738 313 Fragments arranged by size: 1119 738 313 5 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2020 2175 Size: 2020 155 Tth111I GACn'n_nGTC Cuts at: 0 1750 2175 Size: 1750 425 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 64 888 1051 2175 Size: 64 824 163 1124 Fragments arranged by size: 1124 824 163 64 XmaI C'CCGG_G Cuts at: 0 1133 2175 Size: 1133 1042 XmnI GAAnn'nnTTC Cuts at: 0 317 2175 Size: 317 1858 ZraI GAC'GTC Cuts at: 0 1140 2175 Size: 1140 1035 Enzymes that do cut: AatII AccI AcuI AflIII AlfI AlwNI ApaI ApoI AvaI BanI BanII BbsI BbvCI BfrBI BglII BlpI Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BstAPI BstBI BstXI BstYI BtgI BtsI DraI EaeI EarI EciI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HincII HpaI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NsiI NspI PasI PspOMI PstI PvuII SacI SapI SfcI SmaI SmlI StuI StyI TaqII Tth111I XcmI XmaI XmnI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BclI BglI BmgBI BplI BsaAI BsaBI BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI EagI EcoNI EcoRI EcoRV FseI FspI FspAI HindIII KasI KpnI MfeI NaeI NarI NgoMIV NotI NruI PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI XbaI XhoI