(Circular) (Six-base) MAPSORT of: pFN21AB6168.seq Check: 9789 from: 1 to: 6514 pFN21AB6168 6514 bp With 182 enzymes: SgfI * August 27, 2010 15:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2963 3473 2963 Size: 510 6004 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4324 278 Size: 53 83 186 936 2788 2468 Fragments arranged by size: 2788 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2729 3114 3857 1937 Size: 792 385 743 4594 Fragments arranged by size: 4594 792 743 385 Acc65I G'GTAC_C Cuts at: 3835 3835 Size: 6514 AclI AA'CG_TT Cuts at: 1563 4641 5014 1563 Size: 3078 373 3063 Fragments arranged by size: 3078 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2846 3497 4577 5961 6229 1632 Size: 1214 651 1080 1384 268 1917 Fragments arranged by size: 1917 1384 1214 1080 651 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6317 AflIII A'CryG_T Cuts at: 1203 5413 6390 1203 Size: 4210 977 1327 Fragments arranged by size: 4210 1327 977 AgeI A'CCGG_T Cuts at: 5345 5345 Size: 6514 AhdI GACnn_n'nnGTC Cuts at: 5243 5243 Size: 6514 AleI CACnn'nnGTG Cuts at: 1166 3126 3761 1166 Size: 1960 635 3919 Fragments arranged by size: 3919 1960 635 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2270 1184 Size: 1086 5428 AlwNI CAG_nnn'CTG Cuts at: 2021 2503 3145 4257 5829 2021 Size: 482 642 1112 1572 2706 Fragments arranged by size: 2706 1572 1112 642 482 ApaLI G'TGCA_C Cuts at: 4571 5727 4571 Size: 1156 5358 ApoI r'AATT_y Cuts at: 1493 3823 4049 4201 6310 1493 Size: 2330 226 152 2109 1697 Fragments arranged by size: 2330 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5068 160 Size: 872 4036 1606 Fragments arranged by size: 4036 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6514 AvaI C'yCGr_G Cuts at: 1870 1943 2663 3069 3839 1870 Size: 73 720 406 770 4545 Fragments arranged by size: 4545 770 720 406 73 BamHI G'GATC_C Cuts at: 3208 3844 4211 3208 Size: 636 367 5511 Fragments arranged by size: 5511 636 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2041 3485 3835 5290 618 Size: 325 217 627 254 1444 350 1455 1842 Fragments arranged by size: 1842 1455 1444 627 350 325 254 217 BanII G_rGCy'C Cuts at: 1391 2095 2400 3519 3782 3833 1391 Size: 704 305 1119 263 51 4072 Fragments arranged by size: 4072 1119 704 305 263 51 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2636 928 Size: 971 737 4806 Fragments arranged by size: 4806 971 737 BbvCI CC'TCA_GC Cuts at: 3204 3578 3204 Size: 374 6140 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4705 4739 1903 Size: 34 2768 34 3678 Fragments arranged by size: 3678 2768 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4406 5616 4406 Size: 1210 5304 BclI T'GATC_A Cuts at: 1548 1548 Size: 6514 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5125 136 Size: 107 122 71 631 605 3453 1525 Fragments arranged by size: 3453 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2211 2563 6509 2211 Size: 352 3946 2216 Fragments arranged by size: 3946 2216 352 BlpI GC'TnA_GC Cuts at: 2672 3776 3931 2672 Size: 1104 155 5255 Fragments arranged by size: 5255 1104 155 Bme1580I G_kGCm'C Cuts at: 1790 2044 2688 3638 4575 5731 1790 Size: 254 644 950 937 1156 2573 Fragments arranged by size: 2573 1156 950 937 644 254 BmgBI CAC'GTC Cuts at: 1318 2341 1318 Size: 1023 5491 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4245 5203 442 Size: 416 53 83 399 24 2828 958 1753 Fragments arranged by size: 2828 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6514 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2441 2588 3725 5174 1239 Size: 1202 147 1137 1449 2579 Fragments arranged by size: 2579 1449 1202 1137 147 Bpu10I CC'TnA_GC Cuts at: 3060 3204 3578 3060 Size: 144 374 5996 Fragments arranged by size: 5996 374 144 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2258 2519 3788 4639 5504 5802 2258 Size: 261 1269 851 865 298 2970 Fragments arranged by size: 2970 1269 865 851 298 261 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2418 2523 2771 5177 882 Size: 617 919 105 248 2406 2219 Fragments arranged by size: 2406 2219 919 617 248 105 BsaAI yAC'GTr Cuts at: 493 1204 4305 493 Size: 711 3101 2702 Fragments arranged by size: 3101 2702 711 BsaBI GATnn'nnATC Cuts at: 6508 6508 Size: 6514 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4321 4703 5338 275 Size: 53 83 186 936 2788 382 635 1451 Fragments arranged by size: 2788 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2787 3012 4947 5345 5619 5766 6177 2787 Size: 225 1935 398 274 147 411 3124 Fragments arranged by size: 3124 1935 411 398 274 225 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2282 2312 1178 Size: 30 165 30 879 30 5380 Fragments arranged by size: 5380 879 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2449 3212 3722 1186 Size: 1263 763 510 3978 Fragments arranged by size: 3978 1263 763 510 BseYI C'CCAG_C Cuts at: 2539 2990 3005 5717 6213 2539 Size: 451 15 2712 496 2840 Fragments arranged by size: 2840 2712 496 451 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2411 2489 3131 3557 3614 1163 Size: 573 675 78 642 426 57 4063 Fragments arranged by size: 4063 675 642 573 426 78 57 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2049 3964 4725 5753 1540 Size: 275 186 48 1915 761 1028 2301 Fragments arranged by size: 2301 1915 1028 761 275 186 48 BsiHKAI G_wGCw'C Cuts at: 2095 2400 3309 3471 3519 3782 4575 4660 5731 Size: 305 909 162 48 263 793 85 1071 Cuts at: 5731 2095 Size: 2878 Fragments arranged by size: 2878 1071 909 793 305 263 162 85 48 BsmI GAATG_Cn' Cuts at: 4025 4118 4025 Size: 93 6421 BsmBI CGTCTCn'nnnn_ Cuts at: 2948 3806 2948 Size: 858 5656 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2044 2095 2400 2688 3309 3471 3519 Size: 399 254 51 305 288 621 162 48 Cuts at: 3519 3638 3782 3833 4575 4660 5731 1391 Size: 119 144 51 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 621 399 305 288 254 162 144 119 85 51 51 48 BspEI T'CCGG_A Cuts at: 2787 6177 2787 Size: 3390 3124 BspHI T'CATG_A Cuts at: 4403 6133 4403 Size: 1730 4784 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2511 2963 3473 3869 844 Size: 1667 452 510 396 3489 Fragments arranged by size: 3489 1667 510 452 396 BsrBI CCG'CTC Cuts at: 1394 1823 4401 1394 Size: 429 2578 3507 Fragments arranged by size: 3507 2578 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5009 5183 65 Size: 1183 3761 174 1396 Fragments arranged by size: 3761 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2044 2110 2151 3738 5158 5345 1700 Size: 110 234 66 41 1587 1420 187 2869 Fragments arranged by size: 2869 1587 1420 234 187 110 66 41 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6514 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6514 BssSI C'ACGA_G Cuts at: 4574 5586 4574 Size: 1012 5502 BstAPI GCAn_nnn'nTGC Cuts at: 794 2021 2485 3145 794 Size: 1227 464 660 4163 Fragments arranged by size: 4163 1227 660 464 BstBI TT'CG_AA Cuts at: 5318 5318 Size: 6514 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6514 BstXI CCAn_nnnn'nTGG Cuts at: 2998 2998 Size: 6514 BstYI r'GATC_y Cuts at: 1972 2121 2211 2563 2869 3208 3844 4211 4596 Size: 149 90 352 306 339 636 367 385 Cuts at: 4596 4613 6065 6151 6163 6509 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 636 385 367 352 346 339 306 149 90 86 17 12 BstZ17I GTA'TAC Cuts at: 2730 2730 Size: 6514 Bsu36I CC'TnA_GG Cuts at: 2457 2457 Size: 6514 BtgI C'CryG_G Cuts at: 513 1065 2301 2353 2832 513 Size: 552 1236 52 479 4195 Fragments arranged by size: 4195 1236 552 479 52 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5775 Fragments arranged by size: 5775 683 56 BtsI GCAGTG_nn' Cuts at: 2022 2874 4030 4824 4844 2022 Size: 852 1156 794 20 3692 Fragments arranged by size: 3692 1156 852 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6514 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6479 DraI TTT'AAA Cuts at: 3818 3818 Size: 6514 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5521 809 Size: 507 4205 1802 Fragments arranged by size: 4205 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2835 3732 Size: 54 1425 50 275 18 216 789 897 Cuts at: 3732 3789 3961 4850 6479 8 Size: 57 172 889 1629 43 Fragments arranged by size: 1629 1425 897 889 789 275 216 172 57 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2046 3961 1537 Size: 275 234 1915 4090 Fragments arranged by size: 4090 1915 275 234 EarI CTCTTCn'nnn_ Cuts at: 1353 3314 4444 1353 Size: 1961 1130 3423 Fragments arranged by size: 3423 1961 1130 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5101 5475 5621 387 Size: 1064 138 3512 374 146 1280 Fragments arranged by size: 3512 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2093 2398 3517 3780 2093 Size: 305 1119 263 4827 Fragments arranged by size: 4827 1119 305 263 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2441 2588 2846 3497 3725 4577 5174 Size: 393 809 147 258 651 228 852 597 Cuts at: 5174 5961 6229 1239 Size: 787 268 1524 Fragments arranged by size: 1524 852 809 787 651 597 393 268 258 228 147 EcoO109I rG'GnC_Cy Cuts at: 2068 2131 2068 Size: 63 6451 EcoRI G'AATT_C Cuts at: 3823 4201 3823 Size: 378 6136 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2209 3646 3694 2209 Size: 1437 48 5029 Fragments arranged by size: 5029 1437 48 FspI TGC'GCA Cuts at: 5020 5020 Size: 6514 HaeII r_GCGC'y Cuts at: 1678 2161 3564 5661 1678 Size: 483 1403 2097 2531 Fragments arranged by size: 2531 2097 1403 483 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2282 2314 2693 2725 3391 Size: 32 135 32 710 32 379 32 666 Cuts at: 3391 3423 5155 5187 5229 5261 1373 Size: 32 1732 32 42 32 2626 Fragments arranged by size: 2626 1732 710 666 379 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3115 3858 4104 669 Size: 988 281 1177 743 246 3079 Fragments arranged by size: 3079 1177 988 743 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6514 HpaI GTT'AAC Cuts at: 4104 4104 Size: 6514 KpnI G_GTAC'C Cuts at: 3839 3839 Size: 6514 MfeI C'AATT_G Cuts at: 4113 4113 Size: 6514 MluI A'CGCG_T Cuts at: 6390 6390 Size: 6514 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2373 5628 5812 1305 Size: 1068 3255 184 2007 Fragments arranged by size: 3255 2007 1068 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3734 10 Size: 54 1425 343 1902 2790 Fragments arranged by size: 2790 1902 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3126 3761 4472 4831 4990 518 Size: 648 1960 635 711 359 159 2042 Fragments arranged by size: 2042 1960 711 648 635 359 159 MspA1I CmG'CkG Cuts at: 1606 3145 3930 4607 5755 6000 1606 Size: 1539 785 677 1148 245 2120 Fragments arranged by size: 2120 1539 1148 785 677 245 NaeI GCC'GGC Cuts at: 2153 3740 2153 Size: 1587 4927 NcoI C'CATG_G Cuts at: 513 1065 2301 513 Size: 552 1236 4726 Fragments arranged by size: 4726 1236 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6514 NgoMIV G'CCGG_C Cuts at: 2151 3738 2151 Size: 1587 4927 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6514 NotI GC'GGCC_GC Cuts at: 3961 3961 Size: 6514 NspI r_CATG'y Cuts at: 1132 1229 3136 3872 5417 1132 Size: 97 1907 736 1545 2229 Fragments arranged by size: 2229 1907 1545 736 97 PasI CC'CwG_GG Cuts at: 1348 2054 1348 Size: 706 5808 PciI A'CATG_T Cuts at: 5413 5413 Size: 6514 PflMI CCAn_nnn'nTGG Cuts at: 1108 3231 1108 Size: 2123 4391 PfoI T'CCnGG_A Cuts at: 2086 2124 2418 3609 3702 2086 Size: 38 294 1191 93 4898 Fragments arranged by size: 4898 1191 294 93 38 PmeI GTTT'AAAC Cuts at: 3818 3818 Size: 6514 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4583 4615 6096 6128 4583 Size: 32 1481 32 4969 Fragments arranged by size: 4969 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2068 2131 2068 Size: 63 6451 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6514 PsiI TTA'TAA Cuts at: 4084 4084 Size: 6514 PstI C_TGCA'G Cuts at: 830 2017 2026 3141 3360 3866 830 Size: 1187 9 1115 219 506 3478 Fragments arranged by size: 3478 1187 1115 506 219 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6514 PvuII CAG'CTG Cuts at: 3145 3145 Size: 6514 SacI G_AGCT'C Cuts at: 2095 2400 3519 3782 2095 Size: 305 1119 263 4827 Fragments arranged by size: 4827 1119 305 263 SalI G'TCGA_C Cuts at: 1936 3113 3856 1936 Size: 1177 743 4594 Fragments arranged by size: 4594 1177 743 SanDI GG'GwC_CC Cuts at: 2068 2068 Size: 6514 SapI GCTCTTCn'nnn_ Cuts at: 3314 3314 Size: 6514 SbfI CC_TGCA'GG Cuts at: 3866 3866 Size: 6514 ScaI AGT'ACT Cuts at: 4762 6474 4762 Size: 1712 4802 SexAI A'CCwGG_T Cuts at: 3542 3542 Size: 6514 SfcI C'TryA_G Cuts at: 826 1045 2013 2022 3137 3356 3862 4997 5678 Size: 219 968 9 1115 219 506 1135 681 Cuts at: 5678 5869 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1115 968 681 506 219 219 191 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6514 SgrAI Cr'CCGG_yG Cuts at: 1700 2110 1700 Size: 410 6104 SmaI CCC'GGG Cuts at: 1872 3071 3841 1872 Size: 1199 770 4545 Fragments arranged by size: 4545 1199 770 SmlI C'TyrA_G Cuts at: 820 1017 1943 2273 2534 3803 4618 5519 5781 Size: 197 926 330 261 1269 815 901 262 Cuts at: 5781 820 Size: 1553 Fragments arranged by size: 1553 1269 926 901 815 330 262 261 197 SnaBI TAC'GTA Cuts at: 493 4305 493 Size: 3812 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6514 SphI G_CATG'C Cuts at: 1132 3872 1132 Size: 2740 3774 SspI AAT'ATT Cuts at: 5 52 3446 4438 5 Size: 47 3394 992 2081 Fragments arranged by size: 3394 2081 992 47 StuI AGG'CCT Cuts at: 1517 3809 1517 Size: 2292 4222 StyI C'CwwG_G Cuts at: 513 1065 2229 2301 2972 3271 3953 513 Size: 552 1164 72 671 299 682 3074 Fragments arranged by size: 3074 1164 682 671 552 299 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2706 3235 4541 4558 4711 4896 5341 Size: 1255 1126 529 1306 17 153 185 445 Cuts at: 5341 325 Size: 1498 Fragments arranged by size: 1498 1306 1255 1126 529 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3360 4760 6472 Size: 274 80 33 51 1443 1383 1400 1712 Cuts at: 6472 96 Size: 138 Fragments arranged by size: 1712 1443 1400 1383 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3315 3347 6474 6506 3315 Size: 32 3127 32 3323 Fragments arranged by size: 3323 3127 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6514 XbaI T'CTAG_A Cuts at: 3850 3850 Size: 6514 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6514 XmaI C'CCGG_G Cuts at: 1870 3069 3839 1870 Size: 1199 770 4545 Fragments arranged by size: 4545 1199 770 XmnI GAAnn'nnTTC Cuts at: 4643 4643 Size: 6514 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4322 276 Size: 53 83 186 936 2788 2468 Fragments arranged by size: 2788 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI DraIII EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SfiI SfoI SrfI SwaI XcmI