Restriction map for
pFN21AE5216
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AclI AcsI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsiEI BsiWI Bsp1407I
BspDI BspHI BspLU11I BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI HaeII HgaI Hin1I
HindIII HpaI HphI KasI KpnI
Ksp632I KspI MamI MboII MfeI
MflI MluI MluNI MscI MseI
MslI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tru9I Tsp45I Tsp509I Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 22 GTCGAC GT^[AC][GT]AC
AccIII 363 TCCGGA T^CCGGA
AfaI 355 GTAC GT^AC
AflIII 154 ACGTGT A^C[GA][CT]GT
AlwI 174 GATCC GGATC(4/5)
AlwNI 475 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 287 CTCGGG C^[CT]CG[GA]G
AviII 572 TGCGCA TGC^GCA
BbvCI 305 CCTCAGC CCTCAGC(-5/-2)
BcnI 104 CCCGG CC^[GC]GG
BpmI 520 CTGGAG CTGGAG(16/14)
BsaWI 363 TCCGGA [AT]^CCGG[AT]
BseAI 363 TCCGGA T^CCGGA
BseRI 178 CTCCTC GAGGAG(10/8)
BsgI 453 CTGCAC GTGCAG(16/14)
BsmFI 169 GGGAC GGGAC(10/14)
BsmI 261 GAATGC GAATGC(1/-1)
BsoBI 287 CTCGGG C^[CT]CG[GA]G
BspEI 363 TCCGGA T^CCGGA
BstEII 133 GGTTACC G^GT[ACGT]ACC
BstF5I 531 CATCC GGATG(2/0)
BstPI 133 GGTTACC G^GT[ACGT]ACC
BstXI 534 CCAGCTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 566 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 566 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 133 GGTTACC G^GT[ACGT]ACC
FokI 531 CATCC GGATG(9/13)
FspI 572 TGCGCA TGC^GCA
HincII 22 GTCGAC GT[CT]^[GA]AC
HindII 22 GTCGAC GT[CT]^[GA]AC
MaeIII 134 GTTAC ^GT[ACGT]AC
MroI 363 TCCGGA T^CCGGA
NciI 104 CCCGG CC^[GC]GG
RsaI 355 GTAC GT^AC
SacI 316 GAGCTC GAGCT^C
SalI 22 GTCGAC G^TCGAC
SmlI 394 CTCAAG C^T[CT][GA]AG
SphI 140 GCATGC GCATG^C
TspRI 370 CACTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BcgI 62 CGAAGGTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
150 CGAGACGTGTGC
BfaI 509 CTAG C^TAG
527 CTAG
BsmBI 58 GAGACG CGTCTC(1/5)
151 GAGACG
BspMI 336 ACCTGC ACCTGC(4/8)
414 ACCTGC
BsrBI 254 CCGCTC CCGCTC(-3/-3)
85 GAGCGG
BsrI 501 ACTGG ACTGG(1/-1)
352 CCAGT
DpnII 94 GATC ^GATC
174 GATC
FauI 253 CCCGC CCCGC(4/6)
167 GCGGG
HapII 105 CCGG C^CGG
364 CCGG
HinfI 229 GACTC G^A[ACGT]TC
392 GACTC
HpaII 105 CCGG C^CGG
364 CCGG
MaeI 509 CTAG C^TAG
527 CTAG
MaeII 154 ACGT A^CGT
236 ACGT
MboI 94 GATC ^GATC
174 GATC
MspI 105 CCGG C^CGG
364 CCGG
NdeII 94 GATC ^GATC
174 GATC
NlaIV 39 GGAGCC GG[ACGT]^[ACGT]CC
408 GGGGCC
NspI 140 GCATGC [GA]CATG^[CT]
556 GCATGT
PleI 229 GACTC GAGTC(4/5)
392 GACTC
PvuII 298 CAGCTG CAG^CTG
535 CAGCTG
Sau3AI 94 GATC ^GATC
174 GATC
SfaNI 98 GCATC GCATC(5/9)
530 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AccII AciI AluI AspHI BanII
BbvI BmyI BsaJI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BssKI BstNI
BstUI Cac8I CfoI Cfr13I DdeI
EcoRII Fnu4HI HaeIII HhaI HinP1I
ItaI MnlI MspA1I MvaI MvnI
MwoI NlaIII PstI Sau96I ScrFI
SfcI TaqI TseI TthHB8I