(Circular) (Six-base) MAPSORT of: pFN21AE5216.seq Check: 4930 from: 1 to: 5302 pFN21AE5216 5302 bp With 182 enzymes: SgfI * January 30, 2014 17:15 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2428 2428 Size: 5302 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3112 278 Size: 53 83 186 936 1576 2468 Fragments arranged by size: 2468 1576 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2028 2645 1937 Size: 91 617 4594 Fragments arranged by size: 4594 617 91 Acc65I G'GTAC_C Cuts at: 2623 2623 Size: 5302 AclI AA'CG_TT Cuts at: 1563 3429 3802 1563 Size: 1866 373 3063 Fragments arranged by size: 3063 1866 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2043 3365 4749 5017 1632 Size: 411 1322 1384 268 1917 Fragments arranged by size: 1917 1384 1322 411 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5105 AflIII A'CryG_T Cuts at: 1203 2159 4201 5178 1203 Size: 956 2042 977 1327 Fragments arranged by size: 2042 1327 977 956 AgeI A'CCGG_T Cuts at: 4133 4133 Size: 5302 AhdI GACnn_n'nnGTC Cuts at: 4031 4031 Size: 5302 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5302 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5302 AlwNI CAG_nnn'CTG Cuts at: 2485 3045 4617 2485 Size: 560 1572 3170 Fragments arranged by size: 3170 1572 560 ApaLI G'TGCA_C Cuts at: 3359 4515 3359 Size: 1156 4146 ApoI r'AATT_y Cuts at: 1493 2611 2837 2989 5098 1493 Size: 1118 226 152 2109 1697 Fragments arranged by size: 2109 1697 1118 226 152 AseI AT'TA_AT Cuts at: 160 1032 3856 160 Size: 872 2824 1606 Fragments arranged by size: 2824 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5302 AvaI C'yCGr_G Cuts at: 1870 1943 2292 2627 1870 Size: 73 349 335 4545 Fragments arranged by size: 4545 349 335 73 BamHI G'GATC_C Cuts at: 2632 2999 2632 Size: 367 4935 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2623 4078 618 Size: 325 217 627 836 1455 1842 Fragments arranged by size: 1842 1455 836 627 325 217 BanII G_rGCy'C Cuts at: 1391 2049 2325 2336 2513 2621 1391 Size: 658 276 11 177 108 4072 Fragments arranged by size: 4072 658 276 177 108 11 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4331 BbvCI CC'TCA_GC Cuts at: 2311 2311 Size: 5302 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2056 2090 2144 2178 3493 3527 1903 Size: 34 119 34 54 34 1315 34 3678 Fragments arranged by size: 3678 1315 119 54 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3194 4404 3194 Size: 1210 4092 BclI T'GATC_A Cuts at: 1548 1548 Size: 5302 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3913 136 Size: 107 122 71 631 605 2241 1525 Fragments arranged by size: 2241 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5297 5297 Size: 5302 BlpI GC'TnA_GC Cuts at: 2719 2719 Size: 5302 Bme1580I G_kGCm'C Cuts at: 1790 3363 4519 1790 Size: 1573 1156 2573 Fragments arranged by size: 2573 1573 1156 BmgBI CAC'GTC Cuts at: 1318 2160 2242 1318 Size: 842 82 4378 Fragments arranged by size: 4378 842 82 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2351 2516 3033 Size: 416 53 83 399 24 934 165 517 Cuts at: 3033 3991 442 Size: 958 1753 Fragments arranged by size: 1753 958 934 517 416 399 165 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5302 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2546 3962 1239 Size: 1307 1416 2579 Fragments arranged by size: 2579 1416 1307 Bpu10I CC'TnA_GC Cuts at: 2311 2311 Size: 5302 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2384 3427 4292 4590 2384 Size: 1043 865 298 3096 Fragments arranged by size: 3096 1043 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3965 882 Size: 617 2466 2219 Fragments arranged by size: 2466 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3093 493 Size: 711 1889 2702 Fragments arranged by size: 2702 1889 711 BsaBI GATnn'nnATC Cuts at: 5296 5296 Size: 5302 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3109 3491 4126 275 Size: 53 83 186 936 1576 382 635 1451 Fragments arranged by size: 1576 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2368 3735 4133 4407 4554 4965 2368 Size: 1367 398 274 147 411 2705 Fragments arranged by size: 2705 1367 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2010 2021 2040 2051 1178 Size: 30 165 30 607 11 19 11 4429 Fragments arranged by size: 4429 607 165 30 30 19 11 11 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2174 1186 Size: 988 4314 BseYI C'CCAG_C Cuts at: 2149 2349 4505 5001 2149 Size: 200 2156 496 2450 Fragments arranged by size: 2450 2156 496 200 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2443 1163 Size: 573 707 4022 Fragments arranged by size: 4022 707 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2752 3513 4541 1540 Size: 275 186 751 761 1028 2301 Fragments arranged by size: 2301 1028 761 751 275 186 BsiHKAI G_wGCw'C Cuts at: 2301 2325 2376 3363 3448 4519 2301 Size: 24 51 987 85 1071 3084 Fragments arranged by size: 3084 1071 987 85 51 24 BsmI GAATG_Cn' Cuts at: 2272 2813 2906 2272 Size: 541 93 4668 Fragments arranged by size: 4668 541 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2057 2150 2057 Size: 93 5209 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 2301 2325 2336 2376 2513 2621 Size: 399 259 252 24 11 40 137 108 Cuts at: 2621 3363 3448 4519 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 259 252 137 108 85 40 24 11 BspEI T'CCGG_A Cuts at: 2368 4965 2368 Size: 2597 2705 BspHI T'CATG_A Cuts at: 3191 4921 3191 Size: 1730 3572 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2350 2428 2657 844 Size: 1506 78 229 3489 Fragments arranged by size: 3489 1506 229 78 BsrBI CCG'CTC Cuts at: 1394 1823 2092 2261 3189 1394 Size: 429 269 169 928 3507 Fragments arranged by size: 3507 928 429 269 169 BsrDI GCAATG_nn' Cuts at: 65 1248 3797 3971 65 Size: 1183 2549 174 1396 Fragments arranged by size: 2549 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3946 4133 1700 Size: 110 2136 187 2869 Fragments arranged by size: 2869 2136 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5302 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5302 BssSI C'ACGA_G Cuts at: 3362 4374 3362 Size: 1012 4290 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5302 BstBI TT'CG_AA Cuts at: 4106 4106 Size: 5302 BstEII G'GTnAC_C Cuts at: 1184 2138 1184 Size: 954 4348 BstXI CCAn_nnnn'nTGG Cuts at: 2546 2546 Size: 5302 BstYI r'GATC_y Cuts at: 1972 2632 2999 3384 3401 4853 4939 4951 5297 Size: 660 367 385 17 1452 86 12 346 Cuts at: 5297 1972 Size: 1977 Fragments arranged by size: 1977 1452 660 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3811 Fragments arranged by size: 3811 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4563 Fragments arranged by size: 4563 683 56 BtsI GCAGTG_nn' Cuts at: 2818 3612 3632 2818 Size: 794 20 4488 Fragments arranged by size: 4488 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5302 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5267 DraI TTT'AAA Cuts at: 2606 2606 Size: 5302 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4309 809 Size: 507 2993 1802 Fragments arranged by size: 2993 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2749 3638 5267 Size: 54 1425 50 275 18 919 889 1629 Cuts at: 5267 8 Size: 43 Fragments arranged by size: 1629 1425 919 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2749 1537 Size: 275 937 4090 Fragments arranged by size: 4090 937 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3232 1353 Size: 1879 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2075 3889 4263 4409 387 Size: 1064 138 486 1814 374 146 1280 Fragments arranged by size: 1814 1280 1064 486 374 146 138 EcoICRI GAG'CTC Cuts at: 2323 2323 Size: 5302 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2043 2546 3365 3962 4749 5017 1239 Size: 393 411 503 819 597 787 268 1524 Fragments arranged by size: 1524 819 787 597 503 411 393 268 EcoO109I rG'GnC_Cy Cuts at: 2572 2572 Size: 5302 EcoRI G'AATT_C Cuts at: 2611 2989 2611 Size: 378 4924 FspI TGC'GCA Cuts at: 2579 3808 2579 Size: 1229 4073 HaeII r_GCGC'y Cuts at: 1678 4449 1678 Size: 2771 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2010 2021 2042 2053 2090 Size: 32 135 32 438 11 21 11 37 Cuts at: 2090 2122 3943 3975 4017 4049 1373 Size: 32 1821 32 42 32 2626 Fragments arranged by size: 2626 1821 438 135 42 37 32 32 32 32 32 21 11 11 HincII GTy'rAC Cuts at: 669 1657 1938 2029 2646 2892 669 Size: 988 281 91 617 246 3079 Fragments arranged by size: 3079 988 617 281 246 91 HindIII A'AGCT_T Cuts at: 748 748 Size: 5302 HpaI GTT'AAC Cuts at: 2892 2892 Size: 5302 KpnI G_GTAC'C Cuts at: 2627 2627 Size: 5302 MfeI C'AATT_G Cuts at: 2901 2901 Size: 5302 MluI A'CGCG_T Cuts at: 5178 5178 Size: 5302 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4416 4600 1305 Size: 3111 184 2007 Fragments arranged by size: 3111 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3480 Fragments arranged by size: 3480 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3260 3619 3778 518 Size: 648 2094 359 159 2042 Fragments arranged by size: 2094 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2200 2305 2542 2718 3395 4543 4788 1606 Size: 594 105 237 176 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 594 245 237 176 105 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3811 Fragments arranged by size: 3811 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5302 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5302 NotI GC'GGCC_GC Cuts at: 2749 2749 Size: 5302 NspI r_CATG'y Cuts at: 1132 1229 2149 2565 2660 4205 1132 Size: 97 920 416 95 1545 2229 Fragments arranged by size: 2229 1545 920 416 97 95 PasI CC'CwG_GG Cuts at: 1348 2329 2410 1348 Size: 981 81 4240 Fragments arranged by size: 4240 981 81 PciI A'CATG_T Cuts at: 4201 4201 Size: 5302 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5302 PmeI GTTT'AAAC Cuts at: 2606 2606 Size: 5302 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3371 3403 4884 4916 3371 Size: 32 1481 32 3757 Fragments arranged by size: 3757 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5302 PsiI TTA'TAA Cuts at: 2872 2872 Size: 5302 PstI C_TGCA'G Cuts at: 830 2079 2392 2589 2654 830 Size: 1249 313 197 65 3478 Fragments arranged by size: 3478 1249 313 197 65 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5302 PvuII CAG'CTG Cuts at: 2305 2542 2305 Size: 237 5065 SacI G_AGCT'C Cuts at: 2325 2325 Size: 5302 SalI G'TCGA_C Cuts at: 1936 2027 2644 1936 Size: 91 617 4594 Fragments arranged by size: 4594 617 91 SbfI CC_TGCA'GG Cuts at: 2654 2654 Size: 5302 ScaI AGT'ACT Cuts at: 3550 5262 3550 Size: 1712 3590 SfcI C'TryA_G Cuts at: 826 1045 2075 2388 2585 2594 2650 3785 4466 Size: 219 1030 313 197 9 56 1135 681 Cuts at: 4466 4657 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1030 681 313 219 197 191 56 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5302 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5302 SmaI CCC'GGG Cuts at: 1872 2629 1872 Size: 757 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2399 3406 4307 4569 820 Size: 197 926 456 1007 901 262 1553 Fragments arranged by size: 1553 1007 926 901 456 262 197 SnaBI TAC'GTA Cuts at: 493 3093 493 Size: 2600 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5302 SphI G_CATG'C Cuts at: 1132 2149 2660 1132 Size: 1017 511 3774 Fragments arranged by size: 3774 1017 511 SspI AAT'ATT Cuts at: 5 52 3226 5 Size: 47 3174 2081 Fragments arranged by size: 3174 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5302 StyI C'CwwG_G Cuts at: 513 1065 2004 2741 513 Size: 552 939 737 3074 Fragments arranged by size: 3074 939 737 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3329 3346 3499 3684 4129 325 Size: 1255 1749 17 153 185 445 1498 Fragments arranged by size: 1749 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3548 5260 96 Size: 274 80 33 51 1443 1571 1712 138 Fragments arranged by size: 1712 1571 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5262 5294 5262 Size: 32 5270 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5302 XbaI T'CTAG_A Cuts at: 2638 2638 Size: 5302 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5302 XmaI C'CCGG_G Cuts at: 1870 2627 1870 Size: 757 4545 XmnI GAAnn'nnTTC Cuts at: 3431 3431 Size: 5302 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3110 276 Size: 53 83 186 936 1576 2468 Fragments arranged by size: 2468 1576 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI