(Linear) (Six-base) MAPSORT of: KIEE5216.seq Check: 233 from: 1 to: 597 KIEE5216.orf With 182 enzymes: SgfI * January 29, 2014 11:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 423 597 Size: 423 174 AccI GT'mk_AC Cuts at: 0 23 597 Size: 23 574 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 38 597 Size: 38 559 AflIII A'CryG_T Cuts at: 0 154 597 Size: 154 443 AlwNI CAG_nnn'CTG Cuts at: 0 480 597 Size: 480 117 AvaI C'yCGr_G Cuts at: 0 287 597 Size: 287 310 BanII G_rGCy'C Cuts at: 0 44 320 331 508 597 Size: 44 276 11 177 89 Fragments arranged by size: 276 177 89 44 11 BbvCI CC'TCA_GC Cuts at: 0 306 597 Size: 306 291 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 51 85 139 173 597 Size: 51 34 54 34 424 Fragments arranged by size: 424 54 51 34 34 BmgBI CAC'GTC Cuts at: 0 155 237 597 Size: 155 82 360 Fragments arranged by size: 360 155 82 BmrI ACTGGGnnnn_n' Cuts at: 0 346 511 597 Size: 346 165 86 Fragments arranged by size: 346 165 86 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 541 597 Size: 541 56 Bpu10I CC'TnA_GC Cuts at: 0 306 597 Size: 306 291 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 379 597 Size: 379 218 BsaWI w'CCGG_w Cuts at: 0 363 597 Size: 363 234 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 5 16 35 46 597 Size: 5 11 19 11 551 Fragments arranged by size: 551 19 11 11 5 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 169 597 Size: 169 428 BseYI C'CCAG_C Cuts at: 0 144 344 597 Size: 144 200 253 Fragments arranged by size: 253 200 144 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 438 597 Size: 438 159 BsiHKAI G_wGCw'C Cuts at: 0 296 320 371 597 Size: 296 24 51 226 Fragments arranged by size: 296 226 51 24 BsmI GAATG_Cn' Cuts at: 0 267 597 Size: 267 330 BsmBI CGTCTCn'nnnn_ Cuts at: 0 52 145 597 Size: 52 93 452 Fragments arranged by size: 452 93 52 Bsp1286I G_dGCh'C Cuts at: 0 44 296 320 331 371 508 597 Size: 44 252 24 11 40 137 89 Fragments arranged by size: 252 137 89 44 40 24 11 BspEI T'CCGG_A Cuts at: 0 363 597 Size: 363 234 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 345 423 597 Size: 345 78 174 Fragments arranged by size: 345 174 78 BsrBI CCG'CTC Cuts at: 0 87 256 597 Size: 87 169 341 Fragments arranged by size: 341 169 87 BstEII G'GTnAC_C Cuts at: 0 133 597 Size: 133 464 BstXI CCAn_nnnn'nTGG Cuts at: 0 541 597 Size: 541 56 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 70 597 Size: 70 527 EcoICRI GAG'CTC Cuts at: 0 318 597 Size: 318 279 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 38 541 597 Size: 38 503 56 Fragments arranged by size: 503 56 38 EcoO109I rG'GnC_Cy Cuts at: 0 567 597 Size: 567 30 FspI TGC'GCA Cuts at: 0 574 597 Size: 574 23 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 5 16 37 48 85 117 597 Size: 5 11 21 11 37 32 480 Fragments arranged by size: 480 37 32 21 11 11 5 HincII GTy'rAC Cuts at: 0 24 597 Size: 24 573 MspA1I CmG'CkG Cuts at: 0 195 300 537 597 Size: 195 105 237 60 Fragments arranged by size: 237 195 105 60 NspI r_CATG'y Cuts at: 0 144 560 597 Size: 144 416 37 Fragments arranged by size: 416 144 37 PasI CC'CwG_GG Cuts at: 0 324 405 597 Size: 324 81 192 Fragments arranged by size: 324 192 81 PstI C_TGCA'G Cuts at: 0 74 387 584 597 Size: 74 313 197 13 Fragments arranged by size: 313 197 74 13 PvuII CAG'CTG Cuts at: 0 300 537 597 Size: 300 237 60 Fragments arranged by size: 300 237 60 SacI G_AGCT'C Cuts at: 0 320 597 Size: 320 277 SalI G'TCGA_C Cuts at: 0 22 597 Size: 22 575 SfcI C'TryA_G Cuts at: 0 70 383 580 589 597 Size: 70 313 197 9 8 Fragments arranged by size: 313 197 70 9 8 SmlI C'TyrA_G Cuts at: 0 394 597 Size: 394 203 SphI G_CATG'C Cuts at: 0 144 597 Size: 144 453 Enzymes that do cut: AarI AccI AcuI AflIII AlwNI AvaI BanII BbvCI BcgI BmgBI BmrI BpmI Bpu10I BpuEI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BstEII BstXI EciI EcoICRI Eco57MI EcoO109I FspI Hin4I HincII MspA1I NspI PasI PstI PvuII SacI SalI SfcI SmlI SphI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmtI BplI BsaI BsaAI BsaBI BsaHI BsiEI BsiWI BspHI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EcoNI EcoRI EcoRV FalI FseI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI