(Circular) (Six-base) MAPSORT of: pFN21AB0904.seq Check: 9628 from: 1 to: 5602 pFN21AB0904 5602 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3412 278 Size: 53 83 186 936 1876 2468 Fragments arranged by size: 2468 1876 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2945 1937 Size: 1008 4594 Acc65I G'GTAC_C Cuts at: 2923 2923 Size: 5602 AclI AA'CG_TT Cuts at: 1563 3729 4102 1563 Size: 2166 373 3063 Fragments arranged by size: 3063 2166 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2093 2197 2512 3665 5049 5317 1632 Size: 461 104 315 1153 1384 268 1917 Fragments arranged by size: 1917 1384 1153 461 315 268 104 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5405 AflIII A'CryG_T Cuts at: 1203 4501 5478 1203 Size: 3298 977 1327 Fragments arranged by size: 3298 1327 977 AgeI A'CCGG_T Cuts at: 4433 4433 Size: 5602 AhdI GACnn_n'nnGTC Cuts at: 4331 4331 Size: 5602 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5602 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5602 AlwNI CAG_nnn'CTG Cuts at: 2591 3345 4917 2591 Size: 754 1572 3276 Fragments arranged by size: 3276 1572 754 ApaI G_GGCC'C Cuts at: 2052 2479 2635 2052 Size: 427 156 5019 Fragments arranged by size: 5019 427 156 ApaLI G'TGCA_C Cuts at: 3659 4815 3659 Size: 1156 4446 ApoI r'AATT_y Cuts at: 1493 2911 3137 3289 5398 1493 Size: 1418 226 152 2109 1697 Fragments arranged by size: 2109 1697 1418 226 152 AseI AT'TA_AT Cuts at: 160 1032 4156 160 Size: 872 3124 1606 Fragments arranged by size: 3124 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5602 AvaI C'yCGr_G Cuts at: 1870 1943 2052 2927 1870 Size: 73 109 875 4545 Fragments arranged by size: 4545 875 109 73 AvrII C'CTAG_G Cuts at: 2374 2374 Size: 5602 BamHI G'GATC_C Cuts at: 2217 2932 3299 2217 Size: 715 367 4520 Fragments arranged by size: 4520 715 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2093 2923 4378 618 Size: 325 217 627 306 830 1455 1842 Fragments arranged by size: 1842 1455 830 627 325 306 217 BanII G_rGCy'C Cuts at: 1391 2052 2479 2635 2921 1391 Size: 661 427 156 286 4072 Fragments arranged by size: 4072 661 427 286 156 BbeI G_GCGC'C Cuts at: 2097 2097 Size: 5602 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4631 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3793 3827 1903 Size: 34 1856 34 3678 Fragments arranged by size: 3678 1856 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2494 3494 4704 2494 Size: 1000 1210 3392 Fragments arranged by size: 3392 1210 1000 BclI T'GATC_A Cuts at: 1548 2463 1548 Size: 915 4687 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2047 2630 4213 Size: 107 122 71 631 605 375 583 1583 Cuts at: 4213 136 Size: 1525 Fragments arranged by size: 1583 1525 631 605 583 375 122 107 71 BglII A'GATC_T Cuts at: 5597 5597 Size: 5602 BlpI GC'TnA_GC Cuts at: 2564 3019 2564 Size: 455 5147 Bme1580I G_kGCm'C Cuts at: 1790 2052 2066 2145 2479 2635 3663 4819 1790 Size: 262 14 79 334 156 1028 1156 2573 Fragments arranged by size: 2573 1156 1028 334 262 156 79 14 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5602 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3333 4291 442 Size: 416 53 83 399 24 1916 958 1753 Fragments arranged by size: 1916 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5602 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4262 1239 Size: 3023 2579 Bpu10I CC'TnA_GC Cuts at: 2346 2346 Size: 5602 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2411 3727 4592 4890 2411 Size: 1316 865 298 3123 Fragments arranged by size: 3123 1316 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4265 882 Size: 617 2766 2219 Fragments arranged by size: 2766 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3393 493 Size: 711 2189 2702 Fragments arranged by size: 2702 2189 711 BsaBI GATnn'nnATC Cuts at: 5596 5596 Size: 5602 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2094 3409 3791 4426 Size: 53 83 186 936 561 1315 382 635 Cuts at: 4426 275 Size: 1451 Fragments arranged by size: 1451 1315 936 635 561 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4035 4433 4707 4854 5265 4035 Size: 398 274 147 411 4372 Fragments arranged by size: 4372 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5377 Fragments arranged by size: 5377 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2266 1186 Size: 1080 4522 BseYI C'CCAG_C Cuts at: 2083 2469 2613 2625 4805 5301 2083 Size: 386 144 12 2180 496 2384 Fragments arranged by size: 2384 2180 496 386 144 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2198 2702 1163 Size: 573 462 504 4063 Fragments arranged by size: 4063 573 504 462 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2796 3052 3813 4841 1540 Size: 275 186 795 256 761 1028 2301 Fragments arranged by size: 2301 1028 795 761 275 256 186 BsiHKAI G_wGCw'C Cuts at: 2427 3663 3748 4819 2427 Size: 1236 85 1071 3210 Fragments arranged by size: 3210 1236 1071 85 BsmI GAATG_Cn' Cuts at: 3113 3206 3113 Size: 93 5509 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2066 2145 2427 2479 2635 2921 Size: 399 262 14 79 282 52 156 286 Cuts at: 2921 3663 3748 4819 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 286 282 262 156 85 79 52 14 BspEI T'CCGG_A Cuts at: 5265 5265 Size: 5602 BspHI T'CATG_A Cuts at: 3491 5221 3491 Size: 1730 3872 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2957 844 Size: 2113 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3489 1394 Size: 429 1666 3507 Fragments arranged by size: 3507 1666 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4097 4271 65 Size: 1183 2849 174 1396 Fragments arranged by size: 2849 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2090 4246 4433 1700 Size: 110 280 2156 187 2869 Fragments arranged by size: 2869 2156 280 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5602 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5602 BssSI C'ACGA_G Cuts at: 3662 4674 3662 Size: 1012 4590 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5602 BstBI TT'CG_AA Cuts at: 4406 4406 Size: 5602 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5602 BstXI CCAn_nnnn'nTGG Cuts at: 2608 2608 Size: 5602 BstYI r'GATC_y Cuts at: 1972 2217 2932 3299 3684 3701 5153 5239 5251 Size: 245 715 367 385 17 1452 86 12 Cuts at: 5251 5597 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 715 385 367 346 245 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2097 2130 513 Size: 552 939 93 33 3985 Fragments arranged by size: 3985 939 552 93 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4863 Fragments arranged by size: 4863 683 56 BtsI GCAGTG_nn' Cuts at: 3118 3912 3932 3118 Size: 794 20 4788 Fragments arranged by size: 4788 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5602 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5567 DraI TTT'AAA Cuts at: 2906 2906 Size: 5602 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4609 809 Size: 507 3293 1802 Fragments arranged by size: 3293 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2196 2439 2550 Size: 54 1425 50 275 18 366 243 111 Cuts at: 2550 3049 3938 5567 8 Size: 499 889 1629 43 Fragments arranged by size: 1629 1425 889 499 366 275 243 111 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3049 1537 Size: 275 1237 4090 Fragments arranged by size: 4090 1237 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3532 1353 Size: 2179 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2486 4189 4563 4709 387 Size: 1064 138 897 1703 374 146 1280 Fragments arranged by size: 1703 1280 1064 897 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2093 2197 2512 3665 4262 5049 5317 Size: 393 461 104 315 1153 597 787 268 Cuts at: 5317 1239 Size: 1524 Fragments arranged by size: 1524 1153 787 597 461 393 315 268 104 EcoNI CCTnn'n_nnAGG Cuts at: 2198 2888 2198 Size: 690 4912 EcoO109I rG'GnC_Cy Cuts at: 2049 2203 2049 Size: 154 5448 EcoRI G'AATT_C Cuts at: 2911 3289 2911 Size: 378 5224 FspI TGC'GCA Cuts at: 4108 4108 Size: 5602 HaeII r_GCGC'y Cuts at: 1678 2097 2374 4749 1678 Size: 419 277 2375 2531 Fragments arranged by size: 2531 2375 419 277 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4243 4275 4317 4349 1373 Size: 32 135 32 2671 32 42 32 2626 Fragments arranged by size: 2671 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2416 2946 3192 669 Size: 988 281 478 530 246 3079 Fragments arranged by size: 3079 988 530 478 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5602 HpaI GTT'AAC Cuts at: 3192 3192 Size: 5602 KasI G'GCGC_C Cuts at: 2093 2093 Size: 5602 KpnI G_GTAC'C Cuts at: 2927 2927 Size: 5602 MfeI C'AATT_G Cuts at: 3201 3201 Size: 5602 MluI A'CGCG_T Cuts at: 5478 5478 Size: 5602 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4716 4900 1305 Size: 3411 184 2007 Fragments arranged by size: 3411 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2441 2552 10 Size: 54 1425 343 609 111 3060 Fragments arranged by size: 3060 1425 609 343 111 54 MslI CAynn'nnrTG Cuts at: 518 1166 3560 3919 4078 518 Size: 648 2394 359 159 2042 Fragments arranged by size: 2394 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2461 2473 2588 2629 2668 2828 3018 3695 Size: 855 12 115 41 39 160 190 677 Cuts at: 3695 4843 5088 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 855 677 245 190 160 115 41 39 12 NaeI GCC'GGC Cuts at: 2092 2092 Size: 5602 NarI GG'CG_CC Cuts at: 2094 2094 Size: 5602 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4111 Fragments arranged by size: 4111 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5602 NgoMIV G'CCGG_C Cuts at: 2090 2090 Size: 5602 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5602 NotI GC'GGCC_GC Cuts at: 3049 3049 Size: 5602 NspI r_CATG'y Cuts at: 1132 1229 2365 2960 4505 1132 Size: 97 1136 595 1545 2229 Fragments arranged by size: 2229 1545 1136 595 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5602 PciI A'CATG_T Cuts at: 4501 4501 Size: 5602 PflMI CCAn_nnn'nTGG Cuts at: 1108 2641 1108 Size: 1533 4069 PfoI T'CCnGG_A Cuts at: 2538 2538 Size: 5602 PmeI GTTT'AAAC Cuts at: 2906 2906 Size: 5602 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3671 3703 5184 5216 3671 Size: 32 1481 32 4057 Fragments arranged by size: 4057 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5602 PsiI TTA'TAA Cuts at: 3172 3172 Size: 5602 PspOMI G'GGCC_C Cuts at: 2048 2475 2631 2048 Size: 427 156 5019 Fragments arranged by size: 5019 427 156 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2729 2761 2729 Size: 32 5570 PstI C_TGCA'G Cuts at: 830 2954 830 Size: 2124 3478 PvuI CG_AT'CG Cuts at: 2001 2796 2001 Size: 795 4807 PvuII CAG'CTG Cuts at: 2461 2588 2828 2461 Size: 127 240 5235 Fragments arranged by size: 5235 240 127 SalI G'TCGA_C Cuts at: 1936 2944 1936 Size: 1008 4594 SbfI CC_TGCA'GG Cuts at: 2954 2954 Size: 5602 ScaI AGT'ACT Cuts at: 3850 5562 3850 Size: 1712 3890 SfcI C'TryA_G Cuts at: 826 1045 2283 2300 2592 2849 2950 4085 4766 Size: 219 1238 17 292 257 101 1135 681 Cuts at: 4766 4957 826 Size: 191 1471 Fragments arranged by size: 1471 1238 1135 681 292 257 219 191 101 17 SfiI GGCCn_nnn'nGGCC Cuts at: 2630 2630 Size: 5602 SfoI GGC'GCC Cuts at: 2095 2095 Size: 5602 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5602 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5602 SmaI CCC'GGG Cuts at: 1872 2054 2929 1872 Size: 182 875 4545 Fragments arranged by size: 4545 875 182 SmlI C'TyrA_G Cuts at: 820 1017 1943 2426 3706 4607 4869 820 Size: 197 926 483 1280 901 262 1553 Fragments arranged by size: 1553 1280 926 901 483 262 197 SnaBI TAC'GTA Cuts at: 493 3393 493 Size: 2900 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5602 SphI G_CATG'C Cuts at: 1132 2960 1132 Size: 1828 3774 SspI AAT'ATT Cuts at: 5 52 3526 5 Size: 47 3474 2081 Fragments arranged by size: 3474 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5602 StyI C'CwwG_G Cuts at: 513 1065 2004 2222 2374 2520 3041 513 Size: 552 939 218 152 146 521 3074 Fragments arranged by size: 3074 939 552 521 218 152 146 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2483 3629 3646 3799 3984 4429 325 Size: 1255 903 1146 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1146 903 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2580 3848 5560 Size: 274 80 33 51 1443 603 1268 1712 Cuts at: 5560 96 Size: 138 Fragments arranged by size: 1712 1443 1268 603 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5562 5594 5562 Size: 32 5570 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5602 XbaI T'CTAG_A Cuts at: 2938 2938 Size: 5602 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5602 XmaI C'CCGG_G Cuts at: 1870 2052 2927 1870 Size: 182 875 4545 Fragments arranged by size: 4545 875 182 XmnI GAAnn'nnTTC Cuts at: 3731 3731 Size: 5602 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3410 276 Size: 53 83 186 936 1876 2468 Fragments arranged by size: 2468 1876 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI RsrII SacI SacII SanDI SapI SexAI SrfI SwaI XcmI