################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BfrI BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI Bsp1286I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MamI MluI MroI MspA1I NaeI NarI NcoI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI StuI SwaI TspRI Tth111I Van91I XcmI XhoI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AatII 817 GACGTC GACGT^C AccII 1066 CGCG CG^CG AciI 1065 CCGC CCGC(-3/-1) Asp700I 802 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 5 CCCGGG C^[CT]CG[GA]G BalI 751 TGGCCA TGG^CCA BanI 892 GGTGCC G^G[CT][GA]CC BfaI 82 CTAG C^TAG BglI 1033 GCCATACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BseRI 1075 GAGGAG GAGGAG(10/8) BsgI 233 CTGCAC GTGCAG(16/14) BsoBI 5 CCCGGG C^[CT]CG[GA]G Bsp1407I 512 TGTACA T^GTACA BspMI 915 GCAGGT ACCTGC(4/8) BsrGI 512 TGTACA T^GTACA BstEII 733 GGTGACC G^GT[ACGT]ACC BstPI 733 GGTGACC G^GT[ACGT]ACC BstUI 1066 CGCG CG^CG BstXI 896 CCATTGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EcoO65I 733 GGTGACC G^GT[ACGT]ACC EcoRV 418 GATATC GAT^ATC EcoT22I 33 ATGCAT ATGCA^T FauI 1064 CCCGC CCCGC(4/6) MaeI 82 CTAG C^TAG MaeIII 734 GTGAC ^GT[ACGT]AC MfeI 569 CAATTG C^AATTG MluNI 751 TGGCCA TGG^CCA MscI 751 TGGCCA TGG^CCA MslI 30 CATATGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 569 CAATTG C^AATTG MvnI 1066 CGCG CG^CG NdeI 30 CATATG CA^TATG NsiI 33 ATGCAT ATGCA^T NspI 35 GCATGC [GA]CATG^[CT] PleI 127 GACTC GAGTC(4/5) PshAI 149 GACTTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 341 CTGCAG CTGCA^G SapI 1081 GAAGAGC GCTCTTC(1/4) SfcI 341 CTGCAG C^T[GA][CT]AG SmaI 5 CCCGGG CCC^GGG SmlI 613 CTCAAG C^T[CT][GA]AG SphI 35 GCATGC GCATG^C SspBI 512 TGTACA T^GTACA TaqI 640 TCGA T^CGA Tsp45I 734 GTGAC ^GT[GC]AC TthHB8I 640 TCGA T^CGA XbaI 81 TCTAGA T^CTAGA XmaCI 5 CCCGGG C^CCGGG XmaI 5 CCCGGG C^CCGGG XmnI 802 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcsI 76 GAATTT [GA]^AATT[CT] 877 GAATTT AcyI 700 GACGCC G[GA]^CG[CT]C 817 GACGTC AluI 908 AGCT AG^CT 1085 AGCT ApoI 76 GAATTT [GA]^AATT[CT] 877 GAATTT BclI 479 TGATCA T^GATCA 950 TGATCA BglII 534 AGATCT A^GATCT 922 AGATCT BpmI 766 CTCCAG CTGGAG(16/14) 904 CTGGAG BsaHI 700 GACGCC G[GA]^CG[CT]C 817 GACGTC BsmFI 746 GGGAC GGGAC(10/14) 627 GTCCC BstYI 534 AGATCT [GA]^GATC[CT] 922 AGATCT CfoI 49 GCGC GCG^C 757 GCGC DdeI 206 CTCAG C^T[ACGT]AG 873 CTCAG EaeI 213 TGGCCG [CT]^GGCC[GA] 751 TGGCCA EcoT14I 1007 CCAAGG C^C[AT][AT]GG 1043 CCAAGG FbaI 479 TGATCA T^GATCA 950 TGATCA HaeII 48 GGCGCT [GA]GCGC^[CT] 756 AGCGCC HgaI 700 GACGC GACGC(5/10) 858 GACGC HhaI 49 GCGC GCG^C 757 GCGC Hin1I 700 GACGCC G[GA]^CG[CT]C 817 GACGTC HinP1I 49 GCGC G^CGC 757 GCGC HphI 733 GGTGA GGTGA(8/7) 918 GGTGA MaeII 625 ACGT A^CGT 818 ACGT MflI 534 AGATCT [GA]^GATC[CT] 922 AGATCT SfaNI 762 GATGC GCATC(5/9) 1030 GATGC StyI 1007 CCAAGG C^C[AT][AT]GG 1043 CCAAGG XhoII 534 AGATCT [GA]^GATC[CT] 922 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AfaI AvaII BbvI BcnI BsaJI BsiYI BslI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DpnII EarI EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI Ksp632I MboI MboII MnlI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI TfiI Tru9I TseI Tsp509I