(Circular) (Six-base) MAPSORT of: pFN21AE6342.seq Check: 1605 from: 1 to: 5857 pFN21AE6342 5857 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2911 2911 Size: 5857 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2826 3667 278 Size: 53 83 186 936 1290 841 2468 Fragments arranged by size: 2468 1290 936 841 186 83 53 AccI GT'mk_AC Cuts at: 1937 3200 1937 Size: 1263 4594 Acc65I G'GTAC_C Cuts at: 3178 3178 Size: 5857 AclI AA'CG_TT Cuts at: 1563 3984 4357 1563 Size: 2421 373 3063 Fragments arranged by size: 3063 2421 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3920 5304 5572 1632 Size: 2288 1384 268 1917 Fragments arranged by size: 2288 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5660 AflIII A'CryG_T Cuts at: 1203 4756 5733 1203 Size: 3553 977 1327 Fragments arranged by size: 3553 1327 977 AgeI A'CCGG_T Cuts at: 4688 4688 Size: 5857 AhdI GACnn_n'nnGTC Cuts at: 4586 4586 Size: 5857 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5857 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5857 AlwNI CAG_nnn'CTG Cuts at: 3600 5172 3600 Size: 1572 4285 ApaLI G'TGCA_C Cuts at: 3914 5070 3914 Size: 1156 4701 ApoI r'AATT_y Cuts at: 1493 2081 2882 3166 3392 3544 5653 1493 Size: 588 801 284 226 152 2109 1697 Fragments arranged by size: 2109 1697 801 588 284 226 152 AseI AT'TA_AT Cuts at: 160 1032 4411 160 Size: 872 3379 1606 Fragments arranged by size: 3379 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5857 AvaI C'yCGr_G Cuts at: 1870 1943 2010 3182 1870 Size: 73 67 1172 4545 Fragments arranged by size: 4545 1172 73 67 BamHI G'GATC_C Cuts at: 3187 3554 3187 Size: 367 5490 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2897 3178 4633 618 Size: 325 217 627 1110 281 1455 1842 Fragments arranged by size: 1842 1455 1110 627 325 281 217 BanII G_rGCy'C Cuts at: 1391 3176 1391 Size: 1785 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4886 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4048 4082 1903 Size: 34 2111 34 3678 Fragments arranged by size: 3678 2111 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3749 4959 3749 Size: 1210 4647 BclI T'GATC_A Cuts at: 1548 2484 2955 1548 Size: 936 471 4450 Fragments arranged by size: 4450 936 471 BfrBI ATG'CAT Cuts at: 2040 2040 Size: 5857 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3044 4468 136 Size: 107 122 71 631 605 1372 1424 1525 Fragments arranged by size: 1525 1424 1372 631 605 122 107 71 BglII A'GATC_T Cuts at: 2539 2927 5852 2539 Size: 388 2925 2544 Fragments arranged by size: 2925 2544 388 BlpI GC'TnA_GC Cuts at: 3274 3274 Size: 5857 Bme1580I G_kGCm'C Cuts at: 1790 3918 5074 1790 Size: 2128 1156 2573 Fragments arranged by size: 2573 2128 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5857 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2695 3588 4546 Size: 416 53 83 399 24 1278 893 958 Cuts at: 4546 442 Size: 1753 Fragments arranged by size: 1753 1278 958 893 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5857 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2756 2930 4517 1239 Size: 1517 174 1587 2579 Fragments arranged by size: 2579 1587 1517 174 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2603 3982 4847 5145 2603 Size: 1379 865 298 3315 Fragments arranged by size: 3315 1379 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4520 882 Size: 617 3021 2219 Fragments arranged by size: 3021 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3648 493 Size: 711 2444 2702 Fragments arranged by size: 2702 2444 711 BsaBI GATnn'nnATC Cuts at: 5851 5851 Size: 5857 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2706 2823 3664 4046 Size: 53 83 186 936 1173 117 841 382 Cuts at: 4046 4681 275 Size: 635 1451 Fragments arranged by size: 1451 1173 936 841 635 382 186 117 83 53 BsaWI w'CCGG_w Cuts at: 4290 4688 4962 5109 5520 4290 Size: 398 274 147 411 4627 Fragments arranged by size: 4627 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5632 Fragments arranged by size: 5632 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3095 1186 Size: 1909 3948 BseYI C'CCAG_C Cuts at: 5060 5556 5060 Size: 496 5361 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2223 1163 Size: 573 487 4797 Fragments arranged by size: 4797 573 487 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3307 4068 5096 1540 Size: 275 186 1306 761 1028 2301 Fragments arranged by size: 2301 1306 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3918 4003 5074 3918 Size: 85 1071 4701 Fragments arranged by size: 4701 1071 85 BsmI GAATG_Cn' Cuts at: 3368 3461 3368 Size: 93 5764 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3176 3918 4003 5074 1391 Size: 399 1386 742 85 1071 2174 Fragments arranged by size: 2174 1386 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5520 5520 Size: 5857 BspHI T'CATG_A Cuts at: 3746 5476 3746 Size: 1730 4127 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2911 3212 844 Size: 2067 301 3489 Fragments arranged by size: 3489 2067 301 BsrBI CCG'CTC Cuts at: 1394 1823 3744 1394 Size: 429 1921 3507 Fragments arranged by size: 3507 1921 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4352 4526 65 Size: 1183 3104 174 1396 Fragments arranged by size: 3104 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4501 4688 1700 Size: 110 2691 187 2869 Fragments arranged by size: 2869 2691 187 110 BsrGI T'GTAC_A Cuts at: 96 2517 96 Size: 2421 3436 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5857 BssSI C'ACGA_G Cuts at: 3917 4929 3917 Size: 1012 4845 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5857 BstBI TT'CG_AA Cuts at: 4661 4661 Size: 5857 BstEII G'GTnAC_C Cuts at: 1184 2738 1184 Size: 1554 4303 BstXI CCAn_nnnn'nTGG Cuts at: 2908 2908 Size: 5857 BstYI r'GATC_y Cuts at: 1972 2539 2927 3187 3554 3939 3956 5408 5494 Size: 567 388 260 367 385 17 1452 86 Cuts at: 5494 5506 5852 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 567 388 385 367 346 260 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4366 Fragments arranged by size: 4366 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5118 Fragments arranged by size: 5118 683 56 BtsI GCAGTG_nn' Cuts at: 3373 4167 4187 3373 Size: 794 20 5043 Fragments arranged by size: 5043 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5857 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5822 DraI TTT'AAA Cuts at: 3161 3161 Size: 5857 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4864 809 Size: 507 3548 1802 Fragments arranged by size: 3548 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2218 2756 3304 Size: 54 1425 50 275 18 388 538 548 Cuts at: 3304 4193 5822 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 548 538 388 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3304 1537 Size: 275 1492 4090 Fragments arranged by size: 4090 1492 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2964 3072 3081 3787 1353 Size: 1611 108 9 706 3423 Fragments arranged by size: 3423 1611 706 108 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4444 4818 4964 387 Size: 1064 138 2855 374 146 1280 Fragments arranged by size: 2855 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2756 2930 3920 4517 5304 5572 1239 Size: 393 1124 174 990 597 787 268 1524 Fragments arranged by size: 1524 1124 990 787 597 393 268 174 EcoRI G'AATT_C Cuts at: 3166 3544 3166 Size: 378 5479 EcoRV GAT'ATC Cuts at: 2425 2425 Size: 5857 FspI TGC'GCA Cuts at: 4363 4363 Size: 5857 HaeII r_GCGC'y Cuts at: 1678 2057 2765 5004 1678 Size: 379 708 2239 2531 Fragments arranged by size: 2531 2239 708 379 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2409 2441 2555 2587 3080 Size: 32 135 32 837 32 114 32 493 Cuts at: 3080 3112 4498 4530 4572 4604 1373 Size: 32 1386 32 42 32 2626 Fragments arranged by size: 2626 1386 837 493 135 114 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3201 3447 669 Size: 988 281 1263 246 3079 Fragments arranged by size: 3079 1263 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5857 HpaI GTT'AAC Cuts at: 3447 3447 Size: 5857 KpnI G_GTAC'C Cuts at: 3182 3182 Size: 5857 MfeI C'AATT_G Cuts at: 2574 3456 2574 Size: 882 4975 MluI A'CGCG_T Cuts at: 5733 5733 Size: 5857 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4971 5155 1305 Size: 3666 184 2007 Fragments arranged by size: 3666 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2758 10 Size: 54 1425 343 926 3109 Fragments arranged by size: 3109 1425 926 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2039 3815 4174 4333 518 Size: 648 873 1776 359 159 2042 Fragments arranged by size: 2042 1776 873 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3273 3950 5098 5343 1606 Size: 1667 677 1148 245 2120 Fragments arranged by size: 2120 1667 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4366 Fragments arranged by size: 4366 939 552 NdeI CA'TA_TG Cuts at: 387 2036 387 Size: 1649 4208 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5857 NotI GC'GGCC_GC Cuts at: 3304 3304 Size: 5857 NsiI A_TGCA'T Cuts at: 2042 2042 Size: 5857 NspI r_CATG'y Cuts at: 1132 1229 2044 3215 4760 1132 Size: 97 815 1171 1545 2229 Fragments arranged by size: 2229 1545 1171 815 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5857 PciI A'CATG_T Cuts at: 4756 4756 Size: 5857 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5857 PmeI GTTT'AAAC Cuts at: 3161 3161 Size: 5857 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3926 3958 5439 5471 3926 Size: 32 1481 32 4312 Fragments arranged by size: 4312 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2158 1613 Size: 545 5312 PsiI TTA'TAA Cuts at: 3427 3427 Size: 5857 PstI C_TGCA'G Cuts at: 830 2350 3209 830 Size: 1520 859 3478 Fragments arranged by size: 3478 1520 859 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5857 SalI G'TCGA_C Cuts at: 1936 3199 1936 Size: 1263 4594 SapI GCTCTTCn'nnn_ Cuts at: 3081 3081 Size: 5857 SbfI CC_TGCA'GG Cuts at: 3209 3209 Size: 5857 ScaI AGT'ACT Cuts at: 4105 5817 4105 Size: 1712 4145 SfcI C'TryA_G Cuts at: 826 1045 2346 3205 4340 5021 5212 826 Size: 219 1301 859 1135 681 191 1471 Fragments arranged by size: 1471 1301 1135 859 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5857 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5857 SmaI CCC'GGG Cuts at: 1872 2012 3184 1872 Size: 140 1172 4545 Fragments arranged by size: 4545 1172 140 SmlI C'TyrA_G Cuts at: 820 1017 1943 2618 3961 4862 5124 820 Size: 197 926 675 1343 901 262 1553 Fragments arranged by size: 1553 1343 926 901 675 262 197 SnaBI TAC'GTA Cuts at: 493 3648 493 Size: 3155 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5857 SphI G_CATG'C Cuts at: 1132 2044 3215 1132 Size: 912 1171 3774 Fragments arranged by size: 3774 1171 912 SrfI GCCC'GGGC Cuts at: 2012 2012 Size: 5857 SspI AAT'ATT Cuts at: 5 52 3781 5 Size: 47 3729 2081 Fragments arranged by size: 3729 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5857 StyI C'CwwG_G Cuts at: 513 1065 2004 3012 3048 3296 513 Size: 552 939 1008 36 248 3074 Fragments arranged by size: 3074 1008 939 552 248 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3884 3901 4054 4239 4684 325 Size: 1255 2304 17 153 185 445 1498 Fragments arranged by size: 2304 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2286 2517 4103 Size: 274 80 33 51 1443 309 231 1586 Cuts at: 4103 5815 96 Size: 1712 138 Fragments arranged by size: 1712 1586 1443 309 274 231 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5817 5849 5817 Size: 32 5825 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5857 XbaI T'CTAG_A Cuts at: 2086 3193 2086 Size: 1107 4750 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5857 XmaI C'CCGG_G Cuts at: 1870 2010 3182 1870 Size: 140 1172 4545 Fragments arranged by size: 4545 1172 140 XmnI GAAnn'nnTTC Cuts at: 2811 3986 2811 Size: 1175 4682 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2824 3665 276 Size: 53 83 186 936 1290 841 2468 Fragments arranged by size: 2468 1290 936 841 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SwaI XcmI