Restriction map for
pFN21AE1346
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AfaI AflII AflIII AgeI AhdI
AlwNI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbrPI BcgI BciVI BclI
BfrI BglI BglII BlnI BsaAI
BsaBI BsiEI BsiWI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrGI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HincII HindII
HpaI KpnI KspI MamI MfeI
MluI MluNI MscI MseI MslI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tru9I Tsp509I Tth111I Van91I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccII 272 CGCG CG^CG
AccIII 601 TCCGGA T^CCGGA
AcyI 223 GGCGCC G[GA]^CG[CT]C
Alw44I 423 GTGCAC G^TGCAC
ApaLI 423 GTGCAC G^TGCAC
AspHI 423 GTGCAC G[AT]GC[AT]^C
AvaI 412 CCCGGG C^[CT]CG[GA]G
BanI 223 GGCGCC G^G[CT][GA]CC
BanII 392 GAGCCC G[GA]GC[CT]^C
BbeI 223 GGCGCC GGCGC^C
BbvCI 46 GCTGAGG CCTCAGC(-5/-2)
BfaI 389 CTAG C^TAG
BlpI 339 GCTGAGC GC^T[ACGT]AGC
BpmI 671 CTCCAG CTGGAG(16/14)
Bpu1102I 339 GCTGAGC GC^T[ACGT]AGC
BsaHI 223 GGCGCC G[GA]^CG[CT]C
BsaWI 601 TCCGGA [AT]^CCGG[AT]
BseAI 601 TCCGGA T^CCGGA
BseRI 456 GAGGAG GAGGAG(10/8)
BsiHKAI 423 GTGCAC G[AT]GC[AT]^C
BsoBI 412 CCCGGG C^[CT]CG[GA]G
BspEI 601 TCCGGA T^CCGGA
BspMI 130 GCAGGT ACCTGC(4/8)
BsrDI 190 CATTGC GCAATG(2/0)
BssHII 271 GCGCGC G^CGCGC
BstUI 272 CGCG CG^CG
BstYI 116 AGATCC [GA]^GATC[CT]
CelII 339 GCTGAGC GC^T[ACGT]AGC
DsaI 633 CCACGG C^C[GA][CT]GG
EaeI 431 TGGCCG [CT]^GGCC[GA]
EarI 347 GAAGAG CTCTTC(1/4)
EcoNI 707 CCTGGATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 223 GGCGCC [GA]GCGC^[CT]
HgaI 417 GACGC GACGC(5/10)
Hin1I 223 GGCGCC G[GA]^CG[CT]C
HindIII 729 AAGCTT A^AGCTT
KasI 223 GGCGCC G^GCGCC
Ksp632I 347 GAAGAG CTCTTC(1/4)
MaeI 389 CTAG C^TAG
MaeII 368 ACGT A^CGT
MflI 116 AGATCC [GA]^GATC[CT]
MroI 601 TCCGGA T^CCGGA
MvnI 272 CGCG CG^CG
NaeI 433 GCCGGC GCC^GGC
NarI 223 GGCGCC GG^CGCC
NgoMIV 433 GCCGGC G^CCGGC
SfoI 223 GGCGCC GGC^GCC
SmaI 412 CCCGGG CCC^GGG
SmlI 693 CTCAAG C^T[CT][GA]AG
TaqI 363 TCGA T^CGA
Tsp45I 214 GTCAC ^GT[GC]AC
TthHB8I 363 TCGA T^CGA
XhoII 116 AGATCC [GA]^GATC[CT]
XmaCI 412 CCCGGG C^CCGGG
XmaI 412 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwI 117 GATCC GGATC(4/5)
744 GGATC
AvaII 308 GGACC G^G[AT]CC
564 GGACC
BbsI 166 GAAGAC GAAGAC(2/6)
679 GTCTTC
BmyI 392 GAGCCC G[AGT]GC[ACT]^C
423 GTGCAC
BpuAI 166 GAAGAC GAAGAC(2/6)
679 GTCTTC
BsgI 484 GTGCAG GTGCAG(16/14)
496 GTGCAG
BsmBI 642 CGTCTC CGTCTC(1/5)
240 GAGACG
Bsp1286I 392 GAGCCC G[AGT]GC[ACT]^C
423 GTGCAC
BsrFI 433 GCCGGC [GA]^CCGG[CT]
566 ACCGGC
BsrI 40 ACTGG ACTGG(1/-1)
376 CCAGT
BstF5I 282 CATCC GGATG(2/0)
312 CATCC
Cfr10I 433 GCCGGC [GA]^CCGG[CT]
566 ACCGGC
DraII 82 AGGCCCC [GA]G^G[ACGT]CC[CT]
606 AGGCCCC
EcoO109I 82 AGGCCCC [GA]G^G[ACGT]CC[CT]
606 AGGCCCC
FauI 92 CCCGC CCCGC(4/6)
570 GCGGG
FokI 282 CATCC GGATG(9/13)
312 CATCC
HphI 215 TCACC GGTGA(8/7)
683 TCACC
MaeIII 214 GTCAC ^GT[ACGT]AC
620 GTAAC
PleI 123 GAGTC GAGTC(4/5)
350 GAGTC
PvuII 337 CAGCTG CAG^CTG
487 CAGCTG
SfaNI 521 GCATC GCATC(5/9)
638 GCATC
TfiI 360 GAATC G^A[AT]TC
598 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI BbvI BcnI BsaI
BsaJI BsiYI BslI BsmAI BsmFI
BssKI BstNI Cac8I CfoI Cfr13I
DdeI DpnII EcoRII Fnu4HI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MboI MboII MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
TseI TspRI