(Circular) (Six-base) MAPSORT of: pFN21AB5690.seq Check: 8078 from: 1 to: 6259 pFN21AB5690 6259 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3305 3305 Size: 6259 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2439 4069 278 Size: 53 83 186 936 903 1630 2468 Fragments arranged by size: 2468 1630 936 903 186 83 53 AccI GT'mk_AC Cuts at: 1937 2247 2798 3602 1937 Size: 310 551 804 4594 Fragments arranged by size: 4594 804 551 310 Acc65I G'GTAC_C Cuts at: 3580 3580 Size: 6259 AclI AA'CG_TT Cuts at: 1563 2682 4386 4759 1563 Size: 1119 1704 373 3063 Fragments arranged by size: 3063 1704 1119 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4322 5706 5974 1632 Size: 2690 1384 268 1917 Fragments arranged by size: 2690 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6062 AflIII A'CryG_T Cuts at: 1203 2385 5158 6135 1203 Size: 1182 2773 977 1327 Fragments arranged by size: 2773 1327 1182 977 AgeI A'CCGG_T Cuts at: 5090 5090 Size: 6259 AhdI GACnn_n'nnGTC Cuts at: 4988 4988 Size: 6259 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6259 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2942 1184 Size: 1758 4501 AlwNI CAG_nnn'CTG Cuts at: 3320 3329 3372 4002 5574 3320 Size: 9 43 630 1572 4005 Fragments arranged by size: 4005 1572 630 43 9 ApaLI G'TGCA_C Cuts at: 4316 5472 4316 Size: 1156 5103 ApoI r'AATT_y Cuts at: 1493 2557 2835 2996 3043 3333 3568 3794 3946 Size: 1064 278 161 47 290 235 226 152 Cuts at: 3946 6055 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1064 290 278 235 226 161 152 47 AseI AT'TA_AT Cuts at: 160 1032 4813 160 Size: 872 3781 1606 Fragments arranged by size: 3781 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6259 AvaI C'yCGr_G Cuts at: 1870 1943 2019 2143 2150 3584 1870 Size: 73 76 124 7 1434 4545 Fragments arranged by size: 4545 1434 124 76 73 7 BamHI G'GATC_C Cuts at: 3589 3956 3589 Size: 367 5892 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2222 2918 3227 3580 5035 Size: 325 217 627 435 696 309 353 1455 Cuts at: 5035 618 Size: 1842 Fragments arranged by size: 1842 1455 696 627 435 353 325 309 217 BanII G_rGCy'C Cuts at: 1391 3271 3299 3402 3578 1391 Size: 1880 28 103 176 4072 Fragments arranged by size: 4072 1880 176 103 28 BbeI G_GCGC'C Cuts at: 2226 2226 Size: 6259 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2490 928 Size: 971 591 4697 Fragments arranged by size: 4697 971 591 BbvCI CC'TCA_GC Cuts at: 3135 3135 Size: 6259 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4450 4484 1903 Size: 34 2513 34 3678 Fragments arranged by size: 3678 2513 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4151 5361 4151 Size: 1210 5049 BclI T'GATC_A Cuts at: 1548 1548 Size: 6259 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4870 136 Size: 107 122 71 631 605 3198 1525 Fragments arranged by size: 3198 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6254 6254 Size: 6259 BlpI GC'TnA_GC Cuts at: 3676 3676 Size: 6259 Bme1580I G_kGCm'C Cuts at: 1790 4320 5476 1790 Size: 2530 1156 2573 Fragments arranged by size: 2573 2530 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6259 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2041 3027 3990 Size: 416 53 83 399 24 624 986 963 Cuts at: 3990 4948 442 Size: 958 1753 Fragments arranged by size: 1753 986 963 958 624 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6259 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3467 3467 Size: 6259 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2216 2306 2657 3088 4919 1239 Size: 977 90 351 431 1831 2579 Fragments arranged by size: 2579 1831 977 431 351 90 Bpu10I CC'TnA_GC Cuts at: 3135 3135 Size: 6259 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4384 5249 5547 4384 Size: 865 298 5096 Fragments arranged by size: 5096 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2633 4922 882 Size: 617 1134 2289 2219 Fragments arranged by size: 2289 2219 1134 617 BsaAI yAC'GTr Cuts at: 493 1204 2388 4050 493 Size: 711 1184 1662 2702 Fragments arranged by size: 2702 1662 1184 711 BsaBI GATnn'nnATC Cuts at: 2294 6253 2294 Size: 3959 2300 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2210 2223 2436 4066 Size: 53 83 186 936 677 13 213 1630 Cuts at: 4066 4448 5083 275 Size: 382 635 1451 Fragments arranged by size: 1630 1451 936 677 635 382 213 186 83 53 13 BsaWI w'CCGG_w Cuts at: 3486 4692 5090 5364 5511 5922 3486 Size: 1206 398 274 147 411 3823 Fragments arranged by size: 3823 1206 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2672 2702 1178 Size: 30 165 30 1269 30 4735 Fragments arranged by size: 4735 1269 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6259 BseYI C'CCAG_C Cuts at: 3024 5462 5958 3024 Size: 2438 496 3325 Fragments arranged by size: 3325 2438 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3324 1163 Size: 573 1588 4098 Fragments arranged by size: 4098 1588 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3709 4470 5498 1540 Size: 275 186 1708 761 1028 2301 Fragments arranged by size: 2301 1708 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4320 4405 5476 4320 Size: 85 1071 5103 Fragments arranged by size: 5103 1071 85 BsmI GAATG_Cn' Cuts at: 3770 3863 3770 Size: 93 6166 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3271 3299 3402 3578 4320 4405 5476 Size: 399 1481 28 103 176 742 85 1071 Cuts at: 5476 1391 Size: 2174 Fragments arranged by size: 2174 1481 1071 742 399 176 103 85 28 BspEI T'CCGG_A Cuts at: 5922 5922 Size: 6259 BspHI T'CATG_A Cuts at: 3429 4148 5878 3429 Size: 719 1730 3810 Fragments arranged by size: 3810 1730 719 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2262 3099 3305 3614 844 Size: 1418 837 206 309 3489 Fragments arranged by size: 3489 1418 837 309 206 BsrBI CCG'CTC Cuts at: 1394 1823 2421 3383 4146 1394 Size: 429 598 962 763 3507 Fragments arranged by size: 3507 962 763 598 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2532 4754 4928 65 Size: 1183 1284 2222 174 1396 Fragments arranged by size: 2222 1396 1284 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2708 3164 4903 5090 1700 Size: 110 898 456 1739 187 2869 Fragments arranged by size: 2869 1739 898 456 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6259 BssHII G'CGCG_C Cuts at: 1925 2059 1925 Size: 134 6125 BssSI C'ACGA_G Cuts at: 4319 5331 4319 Size: 1012 5247 BstAPI GCAn_nnn'nTGC Cuts at: 794 3311 3320 794 Size: 2517 9 3733 Fragments arranged by size: 3733 2517 9 BstBI TT'CG_AA Cuts at: 5063 5063 Size: 6259 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6259 BstXI CCAn_nnnn'nTGG Cuts at: 2236 3238 2236 Size: 1002 5257 BstYI r'GATC_y Cuts at: 1972 2726 3589 3956 4341 4358 5810 5896 5908 Size: 754 863 367 385 17 1452 86 12 Cuts at: 5908 6254 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 863 754 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2799 2799 Size: 6259 Bsu36I CC'TnA_GG Cuts at: 3513 3513 Size: 6259 BtgI C'CryG_G Cuts at: 513 1065 2004 2112 2217 2241 2272 2355 3231 Size: 552 939 108 105 24 31 83 876 Cuts at: 3231 513 Size: 3541 Fragments arranged by size: 3541 939 876 552 108 105 83 31 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3533 487 Size: 683 56 2307 3213 Fragments arranged by size: 3213 2307 683 56 BtsI GCAGTG_nn' Cuts at: 3330 3775 4569 4589 3330 Size: 445 794 20 5000 Fragments arranged by size: 5000 794 445 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6259 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2527 2562 529 Size: 35 1963 35 4226 Fragments arranged by size: 4226 1963 35 35 DraI TTT'AAA Cuts at: 3563 3563 Size: 6259 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3515 5266 809 Size: 507 2199 1751 1802 Fragments arranged by size: 2199 1802 1751 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2109 2115 2238 Size: 54 1425 50 275 18 279 6 123 Cuts at: 2238 2269 3706 4595 6224 8 Size: 31 1437 889 1629 43 Fragments arranged by size: 1629 1437 1425 889 279 275 123 54 50 43 31 18 6 EagI C'GGCC_G Cuts at: 1537 1812 3706 1537 Size: 275 1894 4090 Fragments arranged by size: 4090 1894 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2808 3269 4189 1353 Size: 1455 461 920 3423 Fragments arranged by size: 3423 1455 920 461 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4846 5220 5366 387 Size: 1064 138 3257 374 146 1280 Fragments arranged by size: 3257 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2216 2306 2657 3088 4322 4919 5706 Size: 393 584 90 351 431 1234 597 787 Cuts at: 5706 5974 1239 Size: 268 1524 Fragments arranged by size: 1524 1234 787 597 584 431 393 351 268 90 EcoO109I rG'GnC_Cy Cuts at: 2147 2154 2301 3021 2147 Size: 7 147 720 5385 Fragments arranged by size: 5385 720 147 7 EcoRI G'AATT_C Cuts at: 2996 3043 3568 3946 2996 Size: 47 525 378 5309 Fragments arranged by size: 5309 525 378 47 FseI GG_CCGG'CC Cuts at: 3168 3168 Size: 6259 FspI TGC'GCA Cuts at: 4765 4765 Size: 6259 HaeII r_GCGC'y Cuts at: 1678 2027 2170 2189 2226 5406 1678 Size: 349 143 19 37 3180 2531 Fragments arranged by size: 3180 2531 349 143 37 19 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3086 3118 3518 3550 4900 Size: 32 135 32 1514 32 400 32 1350 Cuts at: 4900 4932 4974 5006 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1514 1350 400 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2332 2530 3603 3849 669 Size: 988 281 394 198 1073 246 3079 Fragments arranged by size: 3079 1073 988 394 281 246 198 HindIII A'AGCT_T Cuts at: 748 748 Size: 6259 HpaI GTT'AAC Cuts at: 3849 3849 Size: 6259 KasI G'GCGC_C Cuts at: 2222 2222 Size: 6259 KpnI G_GTAC'C Cuts at: 3584 3584 Size: 6259 MfeI C'AATT_G Cuts at: 3858 3858 Size: 6259 MluI A'CGCG_T Cuts at: 6135 6135 Size: 6259 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5373 5557 1305 Size: 4068 184 2007 Fragments arranged by size: 4068 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2240 10 Size: 54 1425 343 408 4029 Fragments arranged by size: 4029 1425 408 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2696 3236 4217 4576 4735 518 Size: 648 1530 540 981 359 159 2042 Fragments arranged by size: 2042 1530 981 648 540 359 159 MspA1I CmG'CkG Cuts at: 1606 2114 2219 2274 3028 3675 4352 5500 5745 Size: 508 105 55 754 647 677 1148 245 Cuts at: 5745 1606 Size: 2120 Fragments arranged by size: 2120 1148 754 677 647 508 245 105 55 NaeI GCC'GGC Cuts at: 2710 3166 2710 Size: 456 5803 NarI GG'CG_CC Cuts at: 2223 2223 Size: 6259 NcoI C'CATG_G Cuts at: 513 1065 2004 2241 513 Size: 552 939 237 4531 Fragments arranged by size: 4531 939 552 237 NdeI CA'TA_TG Cuts at: 387 387 Size: 6259 NgoMIV G'CCGG_C Cuts at: 2708 3164 2708 Size: 456 5803 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6259 NotI GC'GGCC_GC Cuts at: 3706 3706 Size: 6259 NspI r_CATG'y Cuts at: 1132 1229 3617 5162 1132 Size: 97 2388 1545 2229 Fragments arranged by size: 2388 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6259 PciI A'CATG_T Cuts at: 5158 5158 Size: 6259 PflMI CCAn_nnn'nTGG Cuts at: 1108 3408 1108 Size: 2300 3959 PmeI GTTT'AAAC Cuts at: 3563 3563 Size: 6259 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4328 4360 5841 5873 4328 Size: 32 1481 32 4714 Fragments arranged by size: 4714 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2147 2154 2301 3021 2147 Size: 7 147 720 5385 Fragments arranged by size: 5385 720 147 7 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6259 PsiI TTA'TAA Cuts at: 3829 3829 Size: 6259 PstI C_TGCA'G Cuts at: 830 2259 2882 3325 3611 830 Size: 1429 623 443 286 3478 Fragments arranged by size: 3478 1429 623 443 286 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6259 PvuII CAG'CTG Cuts at: 3028 3028 Size: 6259 SacII CC_GC'GG Cuts at: 2115 2220 2275 2115 Size: 105 55 6099 Fragments arranged by size: 6099 105 55 SalI G'TCGA_C Cuts at: 1936 3601 1936 Size: 1665 4594 SanDI GG'GwC_CC Cuts at: 2147 2154 2301 3021 2147 Size: 7 147 720 5385 Fragments arranged by size: 5385 720 147 7 SbfI CC_TGCA'GG Cuts at: 2259 3611 2259 Size: 1352 4907 ScaI AGT'ACT Cuts at: 4507 6219 4507 Size: 1712 4547 SfcI C'TryA_G Cuts at: 826 1045 2255 2318 2878 3321 3607 4742 5423 Size: 219 1210 63 560 443 286 1135 681 Cuts at: 5423 5614 826 Size: 191 1471 Fragments arranged by size: 1471 1210 1135 681 560 443 286 219 191 63 SfoI GGC'GCC Cuts at: 2224 2224 Size: 6259 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6259 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6259 SmaI CCC'GGG Cuts at: 1872 2021 2145 2152 3586 1872 Size: 149 124 7 1434 4545 Fragments arranged by size: 4545 1434 149 124 7 SmlI C'TyrA_G Cuts at: 820 1017 1943 4363 5264 5526 820 Size: 197 926 2420 901 262 1553 Fragments arranged by size: 2420 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4050 493 Size: 3557 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6259 SphI G_CATG'C Cuts at: 1132 3617 1132 Size: 2485 3774 SrfI GCCC'GGGC Cuts at: 2021 2021 Size: 6259 SspI AAT'ATT Cuts at: 5 52 4183 5 Size: 47 4131 2081 Fragments arranged by size: 4131 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6259 StyI C'CwwG_G Cuts at: 513 1065 2004 2241 2424 3056 3698 513 Size: 552 939 237 183 632 642 3074 Fragments arranged by size: 3074 939 642 632 552 237 183 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2747 4286 4303 4456 4641 5086 325 Size: 1255 1167 1539 17 153 185 445 1498 Fragments arranged by size: 1539 1498 1255 1167 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2448 4505 6217 Size: 274 80 33 51 1443 471 2057 1712 Cuts at: 6217 96 Size: 138 Fragments arranged by size: 2057 1712 1443 471 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6219 6251 6219 Size: 32 6227 Tth111I GACn'n_nGTC Cuts at: 1316 2489 3019 1316 Size: 1173 530 4556 Fragments arranged by size: 4556 1173 530 XbaI T'CTAG_A Cuts at: 3595 3595 Size: 6259 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 3214 2011 Size: 1203 5056 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6259 XmaI C'CCGG_G Cuts at: 1870 2019 2143 2150 3584 1870 Size: 149 124 7 1434 4545 Fragments arranged by size: 4545 1434 149 124 7 XmnI GAAnn'nnTTC Cuts at: 4388 4388 Size: 6259 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2437 4067 276 Size: 53 83 186 936 903 1630 2468 Fragments arranged by size: 2468 1630 936 903 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI DraIII EcoICRI EcoNI EcoRV FalI FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SapI SexAI SfiI SwaI