################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII AccI AclI AflII AgeI AhdI AlwI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BalI BamHI BbeI BbvCI BciVI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaBI BsaI BseRI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrFI BsrGI Bst1107I BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII Eam1105I EarI Eco47III Eco81I EcoNI EcoO109I EcoRV EcoT22I FbaI FseI FspI HaeII HindIII HinfI HpaI KasI Ksp632I MamI MfeI MluNI MscI MseI MunI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PleI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I TspRI Tth111I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### Acc65I 128 GGTACC G^GTACC AccIII 472 TCCGGA T^CCGGA AcsI 538 GAATTC [GA]^AATT[CT] AfaI 129 GTAC GT^AC ApoI 538 GAATTC [GA]^AATT[CT] Asp718I 128 GGTACC G^GTACC BanI 128 GGTACC G^G[CT][GA]CC BbsI 385 GTCTTC GAAGAC(2/6) BcgI 20 CGATACTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 374 CTAG C^TAG BglII 95 AGATCT A^GATCT BpuAI 385 GTCTTC GAAGAC(2/6) BsaWI 472 TCCGGA [AT]^CCGG[AT] BseAI 472 TCCGGA T^CCGGA BsiEI 430 CGGCCG CG[GA][CT]^CG BsmFI 153 GGGAC GGGAC(10/14) BspEI 472 TCCGGA T^CCGGA BspMI 266 ACCTGC ACCTGC(4/8) BsrI 273 CCAGT ACTGG(1/-1) BssHII 238 GCGCGC G^CGCGC BssSI 286 CTCGTG CACGAG(-5/-1) BstBI 391 TTCGAA TT^CGAA BstF5I 333 CATCC GGATG(2/0) BstYI 95 AGATCT [GA]^GATC[CT] DdeI 63 CTCAG C^T[ACGT]AG DrdI 457 GACACCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 430 CGGCCG [CT]^GGCC[GA] EagI 430 CGGCCG C^GGCCG EclXI 430 CGGCCG C^GGCCG Eco52I 430 CGGCCG C^GGCCG EcoRI 538 GAATTC G^AATTC EcoT14I 314 CCTTGG C^C[AT][AT]GG FauI 534 GCGGG CCCGC(4/6) FokI 333 CATCC GGATG(9/13) HincII 3 GTTGAC GT[CT]^[GA]AC HindII 3 GTTGAC GT[CT]^[GA]AC KpnI 128 GGTACC GGTAC^C KspI 584 CCGCGG CCGC^GG MaeI 374 CTAG C^TAG MflI 95 AGATCT [GA]^GATC[CT] MluI 140 ACGCGT A^CGCGT MroI 472 TCCGGA T^CCGGA MslI 552 CACCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeI 85 CATATG CA^TATG NlaIII 382 CATG CATG^ NspV 391 TTCGAA TT^CGAA PvuII 205 CAGCTG CAG^CTG RsaI 129 GTAC GT^AC SacI 411 GAGCTC GAGCT^C SacII 584 CCGCGG CCGC^GG SfaNI 683 GCATC GCATC(5/9) SfuI 391 TTCGAA TT^CGAA StyI 314 CCTTGG C^C[AT][AT]GG TaqI 392 TCGA T^CGA Tsp509I 539 AATT ^AATT TthHB8I 392 TCGA T^CGA XbaI 373 TCTAGA T^CTAGA XhoII 95 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AcyI 58 GACGCC G[GA]^CG[CT]C 640 GGCGTC AflIII 122 ACGTGT A^C[GA][CT]GT 140 ACGCGT Alw44I 298 GTGCAC G^TGCAC 693 GTGCAC ApaLI 298 GTGCAC G^TGCAC 693 GTGCAC AvaI 226 CCCGAG C^[CT]CG[GA]G 364 CCCGAG AvaII 311 GGACC G^G[AT]CC 633 GGTCC BbrPI 121 CACGTG CAC^GTG 166 CACGTG BsaAI 121 CACGTG [CT]AC^GT[GA] 166 CACGTG BsaHI 58 GACGCC G[GA]^CG[CT]C 640 GGCGTC BsgI 559 GTGCAG GTGCAG(16/14) 568 GTGCAG BsiYI 227 CCGAGAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 361 CCTCCCGAGGG BslI 227 CCGAGAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 361 CCTCCCGAGGG BsoBI 226 CCCGAG C^[CT]CG[GA]G 364 CCCGAG BsrBI 615 CCGCTC CCGCTC(-3/-3) 307 GAGCGG BsrDI 11 CATTGC GCAATG(2/0) 663 CATTGC BstAPI 210 GCAGCCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 561 GCAGTATGTGC BstEII 465 GGTCACC G^GT[ACGT]ACC 480 GGTCACC BstPI 465 GGTCACC G^GT[ACGT]ACC 480 GGTCACC DpnII 96 GATC ^GATC 198 GATC DsaI 279 CCACGG C^C[GA][CT]GG 584 CCGCGG EcoO65I 465 GGTCACC G^GT[ACGT]ACC 480 GGTCACC Hin1I 58 GACGCC G[GA]^CG[CT]C 640 GGCGTC MaeII 122 ACGT A^CGT 167 ACGT MaeIII 466 GTCAC ^GT[ACGT]AC 481 GTCAC MboI 96 GATC ^GATC 198 GATC NdeII 96 GATC ^GATC 198 GATC PmaCI 121 CACGTG CAC^GTG 166 CACGTG PmlI 121 CACGTG CAC^GTG 166 CACGTG Sau3AI 96 GATC ^GATC 198 GATC Tsp45I 466 GTCAC ^GT[GC]AC 481 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AccII AciI AluI AspHI BanII BbvI BmyI BpmI BsaJI BsiHKAI Bsp1286I BssKI BstNI BstUI Cac8I CfoI Cfr13I EcoRII Fnu4HI HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI MboII MnlI MspA1I MspI MvaI MvnI MwoI NlaIV PstI Sau96I ScrFI SfcI TseI