(Linear) (Six-base) MAPSORT of: KIEE3943.seq Check: 5143 from: 1 to: 1122 KIEE3943.orf With 182 enzymes: SgfI * March 22, 2012 16:55 .. AatII G_ACGT'C Cuts at: 0 368 1122 Size: 368 754 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 924 1122 Size: 924 198 AlwNI CAG_nnn'CTG Cuts at: 0 92 101 1122 Size: 92 9 1021 Fragments arranged by size: 1021 92 9 ApaLI G'TGCA_C Cuts at: 0 379 1122 Size: 379 743 ApoI r'AATT_y Cuts at: 0 229 1122 Size: 229 893 AvaI C'yCGr_G Cuts at: 0 300 484 550 916 1122 Size: 300 184 66 366 206 Fragments arranged by size: 366 300 206 184 66 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 498 531 1122 Size: 498 33 591 Fragments arranged by size: 591 498 33 BanI G'GyrC_C Cuts at: 0 636 1122 Size: 636 486 BanII G_rGCy'C Cuts at: 0 491 779 1122 Size: 491 288 343 Fragments arranged by size: 491 343 288 BbeI G_GCGC'C Cuts at: 0 640 1122 Size: 640 482 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 917 951 1122 Size: 917 34 171 Fragments arranged by size: 917 171 34 BglI GCCn_nnn'nGGC Cuts at: 0 937 1122 Size: 937 185 Bme1580I G_kGCm'C Cuts at: 0 300 383 685 1122 Size: 300 83 302 437 Fragments arranged by size: 437 302 300 83 BmrI ACTGGGnnnn_n' Cuts at: 0 865 1122 Size: 865 257 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 32 343 391 580 787 793 979 1122 Size: 32 311 48 189 207 6 186 143 Fragments arranged by size: 311 207 189 186 143 48 32 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1060 1122 Size: 1060 62 BsaI GGTCTCn'nnnn_ Cuts at: 0 62 1122 Size: 62 1060 BsaHI Gr'CG_yC Cuts at: 0 365 637 1034 1122 Size: 365 272 397 88 Fragments arranged by size: 397 365 272 88 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 43 976 1122 Size: 43 933 146 Fragments arranged by size: 933 146 43 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 577 1122 Size: 577 545 BsiEI CG_ry'CG Cuts at: 0 941 1122 Size: 941 181 BsiHKAI G_wGCw'C Cuts at: 0 383 491 551 1122 Size: 383 108 60 571 Fragments arranged by size: 571 383 108 60 Bsp1286I G_dGCh'C Cuts at: 0 300 383 491 551 685 779 1122 Size: 300 83 108 60 134 94 343 Fragments arranged by size: 343 300 134 108 94 83 60 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 127 431 1122 Size: 127 304 691 Fragments arranged by size: 691 304 127 BsrBI CCG'CTC Cuts at: 0 345 1122 Size: 345 777 BssHII G'CGCG_C Cuts at: 0 472 579 911 924 973 1043 1122 Size: 472 107 332 13 49 70 79 Fragments arranged by size: 472 332 107 79 70 49 13 BssSI C'ACGA_G Cuts at: 0 1114 1122 Size: 1114 8 BtgI C'CryG_G Cuts at: 0 330 827 1122 Size: 330 497 295 Fragments arranged by size: 497 330 295 BtsI GCAGTG_nn' Cuts at: 0 764 1047 1122 Size: 764 283 75 Fragments arranged by size: 764 283 75 DraIII CAC_nnn'GTG Cuts at: 0 1026 1122 Size: 1026 96 EaeI y'GGCC_r Cuts at: 0 2 263 398 938 1122 Size: 2 261 135 540 184 Fragments arranged by size: 540 261 184 135 2 EagI C'GGCC_G Cuts at: 0 938 1122 Size: 938 184 EarI CTCTTCn'nnn_ Cuts at: 0 352 1122 Size: 352 770 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 40 183 1122 Size: 40 143 939 Fragments arranged by size: 939 143 40 EcoICRI GAG'CTC Cuts at: 0 489 1122 Size: 489 633 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 32 343 391 580 787 793 979 1122 Size: 32 311 48 189 207 6 186 143 Fragments arranged by size: 311 207 189 186 143 48 32 6 EcoNI CCTnn'n_nnAGG Cuts at: 0 991 1122 Size: 991 131 EcoO109I rG'GnC_Cy Cuts at: 0 98 535 1122 Size: 98 437 587 Fragments arranged by size: 587 437 98 FspI TGC'GCA Cuts at: 0 1063 1122 Size: 1063 59 HaeII r_GCGC'y Cuts at: 0 128 432 640 652 1122 Size: 128 304 208 12 470 Fragments arranged by size: 470 304 208 128 12 KasI G'GCGC_C Cuts at: 0 636 1122 Size: 636 486 MspA1I CmG'CkG Cuts at: 0 112 332 479 708 790 1122 Size: 112 220 147 229 82 332 Fragments arranged by size: 332 229 220 147 112 82 NarI GG'CG_CC Cuts at: 0 637 1122 Size: 637 485 NspI r_CATG'y Cuts at: 0 177 1122 Size: 177 945 PasI CC'CwG_GG Cuts at: 0 748 1122 Size: 748 374 PfoI T'CCnGG_A Cuts at: 0 368 1122 Size: 368 754 PpuMI rG'GwC_Cy Cuts at: 0 535 1122 Size: 535 587 PshAI GACnn'nnGTC Cuts at: 0 805 1122 Size: 805 317 PspXI vC'TCGA_Gb Cuts at: 0 550 916 1122 Size: 550 366 206 Fragments arranged by size: 550 366 206 PstI C_TGCA'G Cuts at: 0 97 124 467 815 1122 Size: 97 27 343 348 307 Fragments arranged by size: 348 343 307 97 27 PvuII CAG'CTG Cuts at: 0 479 1122 Size: 479 643 SacI G_AGCT'C Cuts at: 0 491 1122 Size: 491 631 SacII CC_GC'GG Cuts at: 0 333 1122 Size: 333 789 SanDI GG'GwC_CC Cuts at: 0 535 1122 Size: 535 587 SapI GCTCTTCn'nnn_ Cuts at: 0 352 1122 Size: 352 770 SfcI C'TryA_G Cuts at: 0 93 120 463 811 1122 Size: 93 27 343 348 311 Fragments arranged by size: 348 343 311 93 27 SfoI GGC'GCC Cuts at: 0 638 1122 Size: 638 484 SmaI CCC'GGG Cuts at: 0 302 1122 Size: 302 820 SmlI C'TyrA_G Cuts at: 0 550 916 1075 1122 Size: 550 366 159 47 Fragments arranged by size: 550 366 159 47 SphI G_CATG'C Cuts at: 0 177 1122 Size: 177 945 StuI AGG'CCT Cuts at: 0 314 565 1107 1122 Size: 314 251 542 15 Fragments arranged by size: 542 314 251 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 659 1122 Size: 659 463 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 32 64 1122 Size: 32 32 1058 Fragments arranged by size: 1058 32 32 XhoI C'TCGA_G Cuts at: 0 550 916 1122 Size: 550 366 206 Fragments arranged by size: 550 366 206 XmaI C'CCGG_G Cuts at: 0 300 1122 Size: 300 822 ZraI GAC'GTC Cuts at: 0 366 1122 Size: 366 756 Enzymes that do cut: AatII AlfI AlwNI ApaLI ApoI AvaI BaeI BanI BanII BbeI BcgI BglI Bme1580I BmrI BpmI BpuEI BsaI BsaHI BseRI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BssHII BssSI BtgI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII KasI MspA1I NarI NspI PasI PfoI PpuMI PshAI PspXI PstI PvuII SacI SacII SanDI SapI SfcI SfoI SmaI SmlI SphI StuI TaqII TstI XhoI XmaI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AloI ApaI AscI AseI AsiSI AvrII BamHI BbsI BbvCI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I BsaAI BsaBI BsaWI BsaXI BseYI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrFI BsrGI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EcoRI EcoRV FalI FseI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PflMI PmeI PmlI PpiI PsiI PspOMI PsrI PvuI RsrII SalI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI StyI SwaI TatI Tth111I XbaI XcmI XmnI