################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII Acc65I AccI AccII AclI AflII AflIII AgeI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspHI AviII BalI BamHI BanII BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BseRI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I Bsp1407I BspDI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DpnII DraI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI FbaI FseI FspI HindIII KpnI KspI MamI MboI MfeI MflI MluI MluNI MscI MunI MvnI NaeI NdeI NdeII NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI Sau3AI SbfI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccIII 634 TCCGGA T^CCGGA AcsI 862 AAATTT [GA]^AATT[CT] AhdI 1127 GACAGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 862 AAATTT [GA]^AATT[CT] AspEI 1127 GACAGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1247 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1076 CTCGGG C^[CT]CG[GA]G AvrII 576 CCTAGG C^CTAGG BbeI 85 GGCGCC GGCGC^C BcnI 8 CCCGG CC^[GC]GG BglI 131 GCCTGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 576 CCTAGG C^CTAGG BsaI 975 GGTCTC GGTCTC(1/5) BsaWI 634 TCCGGA [AT]^CCGG[AT] BseAI 634 TCCGGA T^CCGGA BsmI 350 GAATGC GAATGC(1/-1) BsoBI 1076 CTCGGG C^[CT]CG[GA]G BspEI 634 TCCGGA T^CCGGA BspHI 180 TCATGA T^CATGA BsrBI 781 CCGCTC CCGCTC(-3/-3) DraII 377 AGGCCCT [GA]G^G[ACGT]CC[CT] DsaI 146 CCATGG C^C[GA][CT]GG EaeI 545 TGGCCG [CT]^GGCC[GA] Eam1105I 1127 GACAGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1265 CTCTTC CTCTTC(1/4) EcoNI 68 CCTGGCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 377 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoRV 1153 GATATC GAT^ATC EcoT22I 445 ATGCAT ATGCA^T HpaI 877 GTTAAC GTT^AAC KasI 85 GGCGCC G^GCGCC Ksp632I 1265 CTCTTC CTCTTC(1/4) MroI 634 TCCGGA T^CCGGA MseI 878 TTAA T^TAA NarI 85 GGCGCC GG^CGCC NciI 8 CCCGG CC^[GC]GG NcoI 146 CCATGG C^CATGG NsiI 445 ATGCAT ATGCA^T PflFI 1247 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 554 CCAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 940 CTGCAG CTGCA^G RcaI 180 TCATGA T^CATGA SapI 1264 GCTCTTC GCTCTTC(1/4) ScaI 222 AGTACT AGT^ACT SfoI 85 GGCGCC GGC^GCC SmlI 795 CTTGAG C^T[CT][GA]AG SphI 447 GCATGC GCATG^C TfiI 656 GAATC G^A[AT]TC Tru9I 878 TTAA T^TAA Tth111I 1247 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 554 CCAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AcyI 85 GGCGCC G[GA]^CG[CT]C 364 GACGCC BfaI 577 CTAG C^TAG 841 CTAG BsaHI 85 GGCGCC G[GA]^CG[CT]C 364 GACGCC BsgI 38 GTGCAG GTGCAG(16/14) 617 GTGCAG BsmAI 976 GTCTC GTCTC(1/5) 1365 GTCTC HgaI 364 GACGC GACGC(5/10) 1251 GCGTC Hin1I 85 GGCGCC G[GA]^CG[CT]C 364 GACGCC HincII 877 GTTAAC GT[CT]^[GA]AC 1135 GTCAAC HindII 877 GTTAAC GT[CT]^[GA]AC 1135 GTCAAC HphI 580 GGTGA GGTGA(8/7) 1173 TCACC MaeI 577 CTAG C^TAG 841 CTAG MboII 753 TCTTC GAAGA(8/7) 1266 TCTTC MslI 175 CACTTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 823 CATGAACATG PleI 550 GACTC GAGTC(4/5) 1109 GAGTC PvuII 926 CAGCTG CAG^CTG 988 CAGCTG StuI 129 AGGCCT AGG^CCT 1072 AGGCCT TaqI 203 TCGA T^CGA 920 TCGA TthHB8I 203 TCGA T^CGA 920 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AciI AfaI AluI AvaII BanI BbvI BpmI BsaJI BsiYI BslI BsmFI BspMI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeII MaeIII MnlI MspA1I MspI MvaI MwoI NlaIII NlaIV NspI RsaI Sau96I ScrFI SfaNI SfcI StyI TseI Tsp45I Tsp509I TspRI