################################## # NO CUT ENZYMES ( 89 enzymes) # ################################## AatII AccI AclI AgeI AscI AseI AsnI AviII AvrII BbrPI BciVI BclI BfaI BglII BlnI BsaAI BsaBI BsiEI BsiWI BsmI Bsp1407I BspDI BspHI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraIII DrdI EagI EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI MaeI MaeII MaeIII MamI MfeI MluI MslI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI ScaI SexAI SfiI SfuI SnaBI SpeI SspBI SspI StuI SwaI TfiI Tsp45I XbaI ################################### # ONE CUT ENZYMES ( 68 enzymes) # ################################### Acc65I 1537 GGTACC G^GTACC AccIII 237 TCCGGA T^CCGGA AcsI 1236 GAATTT [GA]^AATT[CT] AcyI 687 GGCGCC G[GA]^CG[CT]C AfaI 1538 GTAC GT^AC AflII 1128 CTTAAG C^TTAAG AflIII 212 ACATGT A^C[GA][CT]GT AhdI 124 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 811 GTGCAC G^TGCAC AocI 1269 CCTCAGG CC^T[ACGT]AGG Aor51HI 593 AGCGCT AGC^GCT ApaLI 811 GTGCAC G^TGCAC ApoI 1236 GAATTT [GA]^AATT[CT] Asp700I 591 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1537 GGTACC G^GTACC AspEI 124 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 180 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1412 TGGCCA TGG^CCA BamHI 536 GGATCC G^GATCC BbeI 687 GGCGCC GGCGC^C BbsI 1947 GTCTTC GAAGAC(2/6) BbvCI 1254 CCTCAGC CCTCAGC(-5/-2) BcgI 39 CGAGGAGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 1128 CTTAAG C^TTAAG BglI 1345 GCCAGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1164 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1164 GCTGAGC GC^T[ACGT]AGC BpuAI 1947 GTCTTC GAAGAC(2/6) BsaHI 687 GGCGCC G[GA]^CG[CT]C BsaWI 237 TCCGGA [AT]^CCGG[AT] BseAI 237 TCCGGA T^CCGGA BsgI 1318 GTGCAG GTGCAG(16/14) BsmBI 715 GAGACG CGTCTC(1/5) BspEI 237 TCCGGA T^CCGGA BspLU11I 212 ACATGT A^CATGT BsrDI 260 GCAATG GCAATG(2/0) BstXI 920 CCAGGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1269 CCTCAGG CC^T[ACGT]AGG CelII 1164 GCTGAGC GC^T[ACGT]AGC DraI 469 TTTAAA TTT^AAA Eam1105I 124 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1149 GAAGAG CTCTTC(1/4) Eco47III 593 AGCGCT AGC^GCT Eco81I 1269 CCTCAGG CC^T[ACGT]AGG Hin1I 687 GGCGCC G[GA]^CG[CT]C KasI 687 GGCGCC G^GCGCC KpnI 1537 GGTACC GGTAC^C Ksp632I 1149 GAAGAG CTCTTC(1/4) KspI 142 CCGCGG CCGC^GG MluNI 1412 TGGCCA TGG^CCA MroI 237 TCCGGA T^CCGGA MscI 1412 TGGCCA TGG^CCA NarI 687 GGCGCC GG^CGCC PflFI 180 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuII 805 CAGCTG CAG^CTG RsaI 1538 GTAC GT^AC SacI 514 GAGCTC GAGCT^C SacII 142 CCGCGG CCGC^GG SfoI 687 GGCGCC GGC^GCC SgrAI 90 CGCCGGCG C[GA]^CCGG[CT]G SmaI 946 CCCGGG CCC^GGG SphI 432 GCATGC GCATG^C Tsp509I 1237 AATT ^AATT Tth111I 180 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 418 CCAGGAGTGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 946 CCCGGG C^CCGGG XmaI 946 CCCGGG C^CCGGG XmnI 591 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### BsrBI 907 CCGCTC CCGCTC(-3/-3) 493 GAGCGG BsrFI 91 GCCGGC [GA]^CCGG[CT] 1169 GCCGGC BssSI 204 CACGAG CACGAG(-5/-1) 961 CACGAG Cfr10I 91 GCCGGC [GA]^CCGG[CT] 1169 GCCGGC EaeI 1352 TGGCCG [CT]^GGCC[GA] 1412 TGGCCA EcoNI 366 CCTCCTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1254 CCTCAGCCAGG HphI 661 GGTGA GGTGA(8/7) 1362 GGTGA MseI 470 TTAA T^TAA 1129 TTAA NaeI 91 GCCGGC GCC^GGC 1169 GCCGGC NgoMIV 91 GCCGGC G^CCGGC 1169 GCCGGC NspI 212 ACATGT [GA]CATG^[CT] 432 GCATGC PaeR7I 673 CTCGAG C^TCGAG 910 CTCGAG Tru9I 470 TTAA T^TAA 1129 TTAA XhoI 673 CTCGAG C^TCGAG 910 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AccII AciI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII ItaI MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI Sau3AI Sau96I SbfI ScrFI SfaNI SfcI SmlI Sse8387I StyI TaqI TseI TspRI TthHB8I Van91I XhoII