(Circular) (Six-base) MAPSORT of: pFN21AE5717.seq Check: 6333 from: 1 to: 6676 pFN21AE5717 6676 bp With 182 enzymes: SgfI * December 8, 2014 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4486 278 Size: 53 83 186 936 2950 2468 Fragments arranged by size: 2950 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4019 1937 Size: 2082 4594 Acc65I G'GTAC_C Cuts at: 3542 3997 3542 Size: 455 6221 AclI AA'CG_TT Cuts at: 1563 4803 5176 1563 Size: 3240 373 3063 Fragments arranged by size: 3240 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2330 4739 6123 6391 1632 Size: 698 2409 1384 268 1917 Fragments arranged by size: 2409 1917 1384 698 268 AfeI AGC'GCT Cuts at: 2600 2600 Size: 6676 AflII C'TTAA_G Cuts at: 820 1017 3133 820 Size: 197 2116 4363 Fragments arranged by size: 4363 2116 197 AflIII A'CryG_T Cuts at: 1203 2217 5575 6552 1203 Size: 1014 3358 977 1327 Fragments arranged by size: 3358 1327 1014 977 AgeI A'CCGG_T Cuts at: 5507 5507 Size: 6676 AhdI GACnn_n'nnGTC Cuts at: 2134 5405 2134 Size: 3271 3405 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6676 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6676 AlwNI CAG_nnn'CTG Cuts at: 2199 2404 2611 3590 3650 3770 3830 4419 5991 Size: 205 207 979 60 120 60 589 1572 Cuts at: 5991 2199 Size: 2884 Fragments arranged by size: 2884 1572 979 589 207 205 120 60 60 ApaI G_GGCC'C Cuts at: 2868 3039 3534 2868 Size: 171 495 6010 Fragments arranged by size: 6010 495 171 ApaLI G'TGCA_C Cuts at: 2816 4733 5889 2816 Size: 1917 1156 3603 Fragments arranged by size: 3603 1917 1156 ApoI r'AATT_y Cuts at: 1493 3241 3985 4211 4363 6472 1493 Size: 1748 744 226 152 2109 1697 Fragments arranged by size: 2109 1748 1697 744 226 152 AseI AT'TA_AT Cuts at: 160 1032 5230 160 Size: 872 4198 1606 Fragments arranged by size: 4198 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6676 AvaI C'yCGr_G Cuts at: 1870 1943 2678 2915 2951 4001 1870 Size: 73 735 237 36 1050 4545 Fragments arranged by size: 4545 1050 735 237 73 36 BamHI G'GATC_C Cuts at: 2541 4006 4373 2541 Size: 1465 367 4844 Fragments arranged by size: 4844 1465 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2692 3428 3471 3542 3997 Size: 325 217 627 905 736 43 71 455 Cuts at: 3997 5452 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 905 736 627 455 325 217 71 43 BanII G_rGCy'C Cuts at: 1391 2523 2868 2924 3039 3060 3132 3534 3557 Size: 1132 345 56 115 21 72 402 23 Cuts at: 3557 3617 3677 3737 3797 3857 3995 1391 Size: 60 60 60 60 60 138 4072 Fragments arranged by size: 4072 1132 402 345 138 115 72 60 60 60 60 60 56 23 21 BbeI G_GCGC'C Cuts at: 2696 2696 Size: 6676 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3945 928 Size: 971 2046 3659 Fragments arranged by size: 3659 2046 971 BbvCI CC'TCA_GC Cuts at: 3260 3260 Size: 6676 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2033 2067 4867 4901 1903 Size: 34 96 34 2800 34 3678 Fragments arranged by size: 3678 2800 96 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4568 5778 4568 Size: 1210 5466 BclI T'GATC_A Cuts at: 1548 1548 Size: 6676 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3356 5287 136 Size: 107 122 71 631 605 1684 1931 1525 Fragments arranged by size: 1931 1684 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6671 6671 Size: 6676 BlpI GC'TnA_GC Cuts at: 3170 4093 3170 Size: 923 5753 Bme1580I G_kGCm'C Cuts at: 1790 2570 2764 2820 2868 3039 3259 3433 3474 Size: 780 194 56 48 171 220 174 41 Cuts at: 3474 3534 4737 5893 1790 Size: 60 1203 1156 2573 Fragments arranged by size: 2573 1203 1156 780 220 194 174 171 60 56 48 41 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6676 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4407 5365 442 Size: 416 53 83 399 24 2990 958 1753 Fragments arranged by size: 2990 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6676 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2570 2735 2908 3098 3185 5336 1239 Size: 1331 165 173 190 87 2151 2579 Fragments arranged by size: 2579 2151 1331 190 173 165 87 Bpu10I CC'TnA_GC Cuts at: 2456 3260 2456 Size: 804 5872 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2396 2639 4801 5666 5964 2396 Size: 243 2162 865 298 3108 Fragments arranged by size: 3108 2162 865 298 243 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3557 3617 3677 3737 3797 3857 5339 Size: 617 2058 60 60 60 60 60 1482 Cuts at: 5339 882 Size: 2219 Fragments arranged by size: 2219 2058 1482 617 60 60 60 60 60 BsaAI yAC'GTr Cuts at: 493 1204 4467 493 Size: 711 3263 2702 Fragments arranged by size: 3263 2702 711 BsaBI GATnn'nnATC Cuts at: 6670 6670 Size: 6676 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2693 4483 4865 5500 Size: 53 83 186 936 1160 1790 382 635 Cuts at: 5500 275 Size: 1451 Fragments arranged by size: 1790 1451 1160 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2242 5109 5507 5781 5928 6339 2242 Size: 2867 398 274 147 411 2579 Fragments arranged by size: 2867 2579 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2009 2039 2372 2402 3339 Size: 30 165 30 606 30 333 30 937 Cuts at: 3339 3369 1178 Size: 30 4485 Fragments arranged by size: 4485 937 606 333 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2051 2060 2147 2156 2732 2951 3097 3521 Size: 865 9 87 9 576 219 146 424 Cuts at: 3521 1186 Size: 4341 Fragments arranged by size: 4341 865 576 424 219 146 87 9 9 BseYI C'CCAG_C Cuts at: 2162 2527 2662 2671 2894 3039 3398 3575 3580 Size: 365 135 9 223 145 359 177 5 Cuts at: 3580 3640 3695 3760 3875 5879 6375 2162 Size: 60 55 65 115 2004 496 2463 Fragments arranged by size: 2463 2004 496 365 359 223 177 145 135 115 65 60 55 9 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3344 1163 Size: 573 1608 4495 Fragments arranged by size: 4495 1608 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4126 4887 5915 1540 Size: 275 186 2125 761 1028 2301 Fragments arranged by size: 2301 2125 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2523 2584 2685 2820 2835 4737 4822 5893 2523 Size: 61 101 135 15 1902 85 1071 3306 Fragments arranged by size: 3306 1902 1071 135 101 85 61 15 BsmI GAATG_Cn' Cuts at: 4187 4280 4187 Size: 93 6583 BsmBI CGTCTCn'nnnn_ Cuts at: 2714 2714 Size: 6676 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2523 2570 2584 2685 2764 2820 2835 Size: 399 733 47 14 101 79 56 15 Cuts at: 2835 2868 2924 3039 3060 3132 3259 3433 3474 Size: 33 56 115 21 72 127 174 41 Cuts at: 3474 3534 3557 3617 3677 3737 3797 3857 3995 Size: 60 23 60 60 60 60 60 138 Cuts at: 3995 4737 4822 5893 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 733 399 174 138 127 115 101 85 79 72 60 60 60 60 60 60 56 56 47 41 33 23 21 15 14 BspEI T'CCGG_A Cuts at: 2242 6339 2242 Size: 4097 2579 BspHI T'CATG_A Cuts at: 4565 6295 4565 Size: 1730 4946 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3598 3658 3778 3838 4031 844 Size: 2754 60 120 60 193 3489 Fragments arranged by size: 3489 2754 193 120 60 60 BsrBI CCG'CTC Cuts at: 1394 1823 2500 2914 4563 1394 Size: 429 677 414 1649 3507 Fragments arranged by size: 3507 1649 677 429 414 BsrDI GCAATG_nn' Cuts at: 65 1248 2272 5171 5345 65 Size: 1183 1024 2899 174 1396 Fragments arranged by size: 2899 1396 1183 1024 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2096 3174 5320 5507 1700 Size: 110 286 1078 2146 187 2869 Fragments arranged by size: 2869 2146 1078 286 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6676 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6676 BssSI C'ACGA_G Cuts at: 2209 2966 4736 5748 2209 Size: 757 1770 1012 3137 Fragments arranged by size: 3137 1770 1012 757 BstAPI GCAn_nnn'nTGC Cuts at: 794 3590 3650 3770 3830 794 Size: 2796 60 120 60 3640 Fragments arranged by size: 3640 2796 120 60 60 BstBI TT'CG_AA Cuts at: 5480 5480 Size: 6676 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6676 BstXI CCAn_nnnn'nTGG Cuts at: 2932 2932 Size: 6676 BstYI r'GATC_y Cuts at: 1972 2245 2304 2490 2541 4006 4373 4758 4775 Size: 273 59 186 51 1465 367 385 17 Cuts at: 4775 6227 6313 6325 6671 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1465 1452 385 367 346 273 186 86 59 51 17 12 Bsu36I CC'TnA_GG Cuts at: 3275 3275 Size: 6676 BtgI C'CryG_G Cuts at: 513 1065 2004 2147 3044 3109 513 Size: 552 939 143 897 65 4080 Fragments arranged by size: 4080 939 897 552 143 65 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5937 Fragments arranged by size: 5937 683 56 BtsI GCAGTG_nn' Cuts at: 3327 4192 4986 5006 3327 Size: 865 794 20 4997 Fragments arranged by size: 4997 865 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6676 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6641 DraI TTT'AAA Cuts at: 2476 3980 2476 Size: 1504 5172 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5683 809 Size: 507 4367 1802 Fragments arranged by size: 4367 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3357 3417 4123 Size: 54 1425 50 275 18 1527 60 706 Cuts at: 4123 5012 6641 8 Size: 889 1629 43 Fragments arranged by size: 1629 1527 1425 889 706 275 60 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4123 1537 Size: 275 2311 4090 Fragments arranged by size: 4090 2311 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3149 4606 1353 Size: 1796 1457 3423 Fragments arranged by size: 3423 1796 1457 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5263 5637 5783 387 Size: 1064 138 3674 374 146 1280 Fragments arranged by size: 3674 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2521 2521 Size: 6676 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2330 2570 2735 2908 3098 3185 4739 Size: 393 698 240 165 173 190 87 1554 Cuts at: 4739 5336 6123 6391 1239 Size: 597 787 268 1524 Fragments arranged by size: 1554 1524 787 698 597 393 268 240 190 173 165 87 EcoNI CCTnn'n_nnAGG Cuts at: 2375 3263 2375 Size: 888 5788 EcoO109I rG'GnC_Cy Cuts at: 2864 2865 2956 3035 3036 3071 3203 3285 3307 Size: 1 91 79 1 35 132 82 22 Cuts at: 3307 3453 3530 3572 3587 3632 3647 3692 3707 Size: 146 77 42 15 45 15 45 15 Cuts at: 3707 3752 3767 3812 3827 3872 3887 2864 Size: 45 15 45 15 45 15 5653 Fragments arranged by size: 5653 146 132 91 82 79 77 45 45 45 45 45 42 35 22 15 15 15 15 15 15 1 1 EcoRI G'AATT_C Cuts at: 3985 4363 3985 Size: 378 6298 FspI TGC'GCA Cuts at: 5182 5182 Size: 6676 HaeII r_GCGC'y Cuts at: 1678 2602 2696 2806 5823 1678 Size: 924 94 110 3017 2531 Fragments arranged by size: 3017 2531 924 110 94 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2009 2041 2120 2152 2372 Size: 32 135 32 437 32 79 32 220 Cuts at: 2372 2404 3602 3634 3662 3694 3722 3754 3782 Size: 32 1198 32 28 32 28 32 28 Cuts at: 3782 3814 3842 3874 3935 3967 5317 5349 5391 Size: 32 28 32 61 32 1350 32 42 Cuts at: 5391 5423 1373 Size: 32 2626 Fragments arranged by size: 2626 1350 1198 437 220 135 79 61 42 32 32 32 32 32 32 32 32 32 32 32 32 32 28 28 28 28 HincII GTy'rAC Cuts at: 669 1657 1938 4020 4266 669 Size: 988 281 2082 246 3079 Fragments arranged by size: 3079 2082 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6676 HpaI GTT'AAC Cuts at: 4266 4266 Size: 6676 KasI G'GCGC_C Cuts at: 2692 2692 Size: 6676 KpnI G_GTAC'C Cuts at: 3546 4001 3546 Size: 455 6221 MfeI C'AATT_G Cuts at: 4275 4275 Size: 6676 MluI A'CGCG_T Cuts at: 6552 6552 Size: 6676 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2118 2966 5790 5974 1305 Size: 813 848 2824 184 2007 Fragments arranged by size: 2824 2007 848 813 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3419 10 Size: 54 1425 343 1587 3267 Fragments arranged by size: 3267 1587 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4634 4993 5152 518 Size: 648 3468 359 159 2042 Fragments arranged by size: 3468 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2149 2698 2812 2847 4092 4769 5917 6162 Size: 543 549 114 35 1245 677 1148 245 Cuts at: 6162 1606 Size: 2120 Fragments arranged by size: 2120 1245 1148 677 549 543 245 114 35 NaeI GCC'GGC Cuts at: 2098 3176 2098 Size: 1078 5598 NarI GG'CG_CC Cuts at: 2693 2693 Size: 6676 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5185 Fragments arranged by size: 5185 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6676 NgoMIV G'CCGG_C Cuts at: 2096 3174 2096 Size: 1078 5598 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6676 NotI GC'GGCC_GC Cuts at: 4123 4123 Size: 6676 NspI r_CATG'y Cuts at: 1132 1229 2221 2441 4034 5579 1132 Size: 97 992 220 1593 1545 2229 Fragments arranged by size: 2229 1593 1545 992 220 97 PasI CC'CwG_GG Cuts at: 1348 2980 3311 3343 3548 1348 Size: 1632 331 32 205 4476 Fragments arranged by size: 4476 1632 331 205 32 PciI A'CATG_T Cuts at: 2217 5575 2217 Size: 3358 3318 PflMI CCAn_nnn'nTGG Cuts at: 1108 2199 3295 3343 3439 1108 Size: 1091 1096 48 96 4345 Fragments arranged by size: 4345 1096 1091 96 48 PfoI T'CCnGG_A Cuts at: 2493 2712 2924 2493 Size: 219 212 6245 Fragments arranged by size: 6245 219 212 PmeI GTTT'AAAC Cuts at: 3980 3980 Size: 6676 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4745 4777 6258 6290 4745 Size: 32 1481 32 5131 Fragments arranged by size: 5131 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2956 3071 3285 3307 3453 3587 3647 3707 3767 Size: 115 214 22 146 134 60 60 60 Cuts at: 3767 3827 3887 2956 Size: 60 60 5745 Fragments arranged by size: 5745 214 146 134 115 60 60 60 60 60 22 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6676 PsiI TTA'TAA Cuts at: 4246 4246 Size: 6676 PspOMI G'GGCC_C Cuts at: 2864 3035 3530 2864 Size: 171 495 6010 Fragments arranged by size: 6010 495 171 PspXI vC'TCGA_Gb Cuts at: 2678 2915 2678 Size: 237 6439 PstI C_TGCA'G Cuts at: 830 3595 3655 3775 3835 4028 830 Size: 2765 60 120 60 193 3478 Fragments arranged by size: 3478 2765 193 120 60 60 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6676 PvuII CAG'CTG Cuts at: 2812 2812 Size: 6676 SacI G_AGCT'C Cuts at: 2523 2523 Size: 6676 SacII CC_GC'GG Cuts at: 2150 2150 Size: 6676 SalI G'TCGA_C Cuts at: 1936 4018 1936 Size: 2082 4594 SanDI GG'GwC_CC Cuts at: 2956 3071 3285 3453 2956 Size: 115 214 168 6179 Fragments arranged by size: 6179 214 168 115 SbfI CC_TGCA'GG Cuts at: 3595 3655 3775 3835 4028 3595 Size: 60 120 60 193 6243 Fragments arranged by size: 6243 193 120 60 60 ScaI AGT'ACT Cuts at: 4924 6636 4924 Size: 1712 4964 SfcI C'TryA_G Cuts at: 826 1045 2293 3591 3651 3771 3831 4024 5159 Size: 219 1248 1298 60 120 60 193 1135 Cuts at: 5159 5840 6031 826 Size: 681 191 1471 Fragments arranged by size: 1471 1298 1248 1135 681 219 193 191 120 60 60 SfoI GGC'GCC Cuts at: 2694 2694 Size: 6676 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6676 SgrAI Cr'CCGG_yG Cuts at: 1700 2096 1700 Size: 396 6280 SmaI CCC'GGG Cuts at: 1872 2953 4003 1872 Size: 1081 1050 4545 Fragments arranged by size: 4545 1081 1050 SmlI C'TyrA_G Cuts at: 820 1017 1943 2375 2654 2678 2915 3133 4780 Size: 197 926 432 279 24 237 218 1647 Cuts at: 4780 5681 5943 820 Size: 901 262 1553 Fragments arranged by size: 1647 1553 926 901 432 279 262 237 218 197 24 SnaBI TAC'GTA Cuts at: 493 4467 493 Size: 3974 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6676 SphI G_CATG'C Cuts at: 1132 2441 4034 1132 Size: 1309 1593 3774 Fragments arranged by size: 3774 1593 1309 SspI AAT'ATT Cuts at: 5 52 4600 5 Size: 47 4548 2081 Fragments arranged by size: 4548 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6676 StyI C'CwwG_G Cuts at: 513 1065 2004 3438 3477 3909 4115 513 Size: 552 939 1434 39 432 206 3074 Fragments arranged by size: 3074 1434 939 552 432 206 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2571 2654 2993 4703 4720 4873 5058 Size: 1255 991 83 339 1710 17 153 185 Cuts at: 5058 5503 325 Size: 445 1498 Fragments arranged by size: 1710 1498 1255 991 445 339 185 153 83 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4922 6634 96 Size: 274 80 33 51 1443 2945 1712 138 Fragments arranged by size: 2945 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3339 3371 6636 6668 3339 Size: 32 3265 32 3347 Fragments arranged by size: 3347 3265 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2188 1316 Size: 872 5804 XbaI T'CTAG_A Cuts at: 4012 4012 Size: 6676 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2430 2430 Size: 6676 XhoI C'TCGA_G Cuts at: 1943 2678 2915 1943 Size: 735 237 5704 Fragments arranged by size: 5704 735 237 XmaI C'CCGG_G Cuts at: 1870 2951 4001 1870 Size: 1081 1050 4545 Fragments arranged by size: 4545 1081 1050 XmnI GAAnn'nnTTC Cuts at: 2600 4805 2600 Size: 2205 4471 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4484 276 Size: 53 83 186 936 2950 2468 Fragments arranged by size: 2950 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PsrI RsrII SapI SexAI SfiI SrfI SwaI