(Linear) (Six-base) MAPSORT of: KIEE5717.seq Check: 5997 from: 1 to: 1971 KIEE5717.orf With 182 enzymes: SgfI * December 5, 2014 13:07 .. Acc65I G'GTAC_C Cuts at: 0 1537 1971 Size: 1537 434 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 325 1971 Size: 325 1646 AfeI AGC'GCT Cuts at: 0 595 1971 Size: 595 1376 AflII C'TTAA_G Cuts at: 0 1128 1971 Size: 1128 843 AflIII A'CryG_T Cuts at: 0 212 1971 Size: 212 1759 AhdI GACnn_n'nnGTC Cuts at: 0 129 1971 Size: 129 1842 AlwNI CAG_nnn'CTG Cuts at: 0 194 399 606 1585 1645 1765 1825 1971 Size: 194 205 207 979 60 120 60 146 Fragments arranged by size: 979 207 205 194 146 120 60 60 ApaI G_GGCC'C Cuts at: 0 863 1034 1529 1971 Size: 863 171 495 442 Fragments arranged by size: 863 495 442 171 ApaLI G'TGCA_C Cuts at: 0 811 1971 Size: 811 1160 ApoI r'AATT_y Cuts at: 0 1236 1971 Size: 1236 735 AvaI C'yCGr_G Cuts at: 0 673 910 946 1971 Size: 673 237 36 1025 Fragments arranged by size: 1025 673 237 36 BamHI G'GATC_C Cuts at: 0 536 1971 Size: 536 1435 BanI G'GyrC_C Cuts at: 0 687 1423 1466 1537 1971 Size: 687 736 43 71 434 Fragments arranged by size: 736 687 434 71 43 BanII G_rGCy'C Cuts at: 0 518 863 919 1034 1055 1127 1529 1552 Size: 518 345 56 115 21 72 402 23 Cuts at: 1552 1612 1672 1732 1792 1852 1971 Size: 60 60 60 60 60 119 Fragments arranged by size: 518 402 345 119 115 72 60 60 60 60 60 56 23 21 BbeI G_GCGC'C Cuts at: 0 691 1971 Size: 691 1280 BbsI GAAGACnn'nnnn_ Cuts at: 0 1940 1971 Size: 1940 31 BbvCI CC'TCA_GC Cuts at: 0 1255 1971 Size: 1255 716 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 28 62 1971 Size: 28 34 1909 Fragments arranged by size: 1909 34 28 BglI GCCn_nnn'nGGC Cuts at: 0 1351 1971 Size: 1351 620 BlpI GC'TnA_GC Cuts at: 0 1165 1971 Size: 1165 806 Bme1580I G_kGCm'C Cuts at: 0 565 759 815 863 1034 1254 1428 1469 Size: 565 194 56 48 171 220 174 41 Cuts at: 1469 1529 1971 Size: 60 442 Fragments arranged by size: 565 442 220 194 174 171 60 56 48 41 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 565 730 903 1093 1180 1971 Size: 565 165 173 190 87 791 Fragments arranged by size: 791 565 190 173 165 87 Bpu10I CC'TnA_GC Cuts at: 0 451 1255 1971 Size: 451 804 716 Fragments arranged by size: 804 716 451 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 391 634 1971 Size: 391 243 1337 Fragments arranged by size: 1337 391 243 BsaI GGTCTCn'nnnn_ Cuts at: 0 1552 1612 1672 1732 1792 1852 1971 Size: 1552 60 60 60 60 60 119 Fragments arranged by size: 1552 119 60 60 60 60 60 BsaHI Gr'CG_yC Cuts at: 0 688 1971 Size: 688 1283 BsaWI w'CCGG_w Cuts at: 0 237 1971 Size: 237 1734 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 4 34 367 397 1334 1364 1971 Size: 4 30 333 30 937 30 607 Fragments arranged by size: 937 607 333 30 30 30 4 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 46 55 142 151 727 946 1092 1516 Size: 46 9 87 9 576 219 146 424 Cuts at: 1516 1971 Size: 455 Fragments arranged by size: 576 455 424 219 146 87 46 9 9 BseYI C'CCAG_C Cuts at: 0 157 522 657 666 889 1034 1393 1570 Size: 157 365 135 9 223 145 359 177 Cuts at: 1570 1575 1635 1690 1755 1870 1971 Size: 5 60 55 65 115 101 Fragments arranged by size: 365 359 223 177 157 145 135 115 101 65 60 55 9 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1339 1971 Size: 1339 632 BsiHKAI G_wGCw'C Cuts at: 0 518 579 680 815 830 1971 Size: 518 61 101 135 15 1141 Fragments arranged by size: 1141 518 135 101 61 15 BsmBI CGTCTCn'nnnn_ Cuts at: 0 709 1971 Size: 709 1262 Bsp1286I G_dGCh'C Cuts at: 0 518 565 579 680 759 815 830 863 Size: 518 47 14 101 79 56 15 33 Cuts at: 863 919 1034 1055 1127 1254 1428 1469 1529 Size: 56 115 21 72 127 174 41 60 Cuts at: 1529 1552 1612 1672 1732 1792 1852 1971 Size: 23 60 60 60 60 60 119 Fragments arranged by size: 518 174 127 119 115 101 79 72 60 60 60 60 60 60 56 56 47 41 33 23 21 15 14 BspEI T'CCGG_A Cuts at: 0 237 1971 Size: 237 1734 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1593 1653 1773 1833 1971 Size: 1593 60 120 60 138 Fragments arranged by size: 1593 138 120 60 60 BsrBI CCG'CTC Cuts at: 0 495 909 1971 Size: 495 414 1062 Fragments arranged by size: 1062 495 414 BsrDI GCAATG_nn' Cuts at: 0 267 1971 Size: 267 1704 BsrFI r'CCGG_y Cuts at: 0 91 1169 1971 Size: 91 1078 802 Fragments arranged by size: 1078 802 91 BssSI C'ACGA_G Cuts at: 0 204 961 1971 Size: 204 757 1010 Fragments arranged by size: 1010 757 204 BstAPI GCAn_nnn'nTGC Cuts at: 0 1585 1645 1765 1825 1971 Size: 1585 60 120 60 146 Fragments arranged by size: 1585 146 120 60 60 BstXI CCAn_nnnn'nTGG Cuts at: 0 927 1971 Size: 927 1044 BstYI r'GATC_y Cuts at: 0 240 299 485 536 1971 Size: 240 59 186 51 1435 Fragments arranged by size: 1435 240 186 59 51 Bsu36I CC'TnA_GG Cuts at: 0 1270 1971 Size: 1270 701 BtgI C'CryG_G Cuts at: 0 142 1039 1104 1971 Size: 142 897 65 867 Fragments arranged by size: 897 867 142 65 BtsI GCAGTG_nn' Cuts at: 0 1322 1971 Size: 1322 649 DraI TTT'AAA Cuts at: 0 471 1971 Size: 471 1500 EaeI y'GGCC_r Cuts at: 0 1352 1412 1971 Size: 1352 60 559 Fragments arranged by size: 1352 559 60 EarI CTCTTCn'nnn_ Cuts at: 0 1144 1971 Size: 1144 827 EcoICRI GAG'CTC Cuts at: 0 516 1971 Size: 516 1455 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 325 565 730 903 1093 1180 1971 Size: 325 240 165 173 190 87 791 Fragments arranged by size: 791 325 240 190 173 165 87 EcoNI CCTnn'n_nnAGG Cuts at: 0 370 1258 1971 Size: 370 888 713 Fragments arranged by size: 888 713 370 EcoO109I rG'GnC_Cy Cuts at: 0 859 860 951 1030 1031 1066 1198 1280 Size: 859 1 91 79 1 35 132 82 Cuts at: 1280 1302 1448 1525 1567 1582 1627 1642 1687 Size: 22 146 77 42 15 45 15 45 Cuts at: 1687 1702 1747 1762 1807 1822 1867 1882 1971 Size: 15 45 15 45 15 45 15 89 Fragments arranged by size: 859 146 132 91 89 82 79 77 45 45 45 45 45 42 35 22 15 15 15 15 15 15 1 1 HaeII r_GCGC'y Cuts at: 0 597 691 801 1971 Size: 597 94 110 1170 Fragments arranged by size: 1170 597 110 94 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 4 36 115 147 367 399 1597 1629 Size: 4 32 79 32 220 32 1198 32 Cuts at: 1629 1657 1689 1717 1749 1777 1809 1837 1869 Size: 28 32 28 32 28 32 28 32 Cuts at: 1869 1930 1962 1971 Size: 61 32 9 Fragments arranged by size: 1198 220 79 61 32 32 32 32 32 32 32 32 32 28 28 28 28 9 4 KasI G'GCGC_C Cuts at: 0 687 1971 Size: 687 1284 KpnI G_GTAC'C Cuts at: 0 1541 1971 Size: 1541 430 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 113 961 1971 Size: 113 848 1010 Fragments arranged by size: 1010 848 113 MscI TGG'CCA Cuts at: 0 1414 1971 Size: 1414 557 MspA1I CmG'CkG Cuts at: 0 144 693 807 842 1971 Size: 144 549 114 35 1129 Fragments arranged by size: 1129 549 144 114 35 NaeI GCC'GGC Cuts at: 0 93 1171 1971 Size: 93 1078 800 Fragments arranged by size: 1078 800 93 NarI GG'CG_CC Cuts at: 0 688 1971 Size: 688 1283 NgoMIV G'CCGG_C Cuts at: 0 91 1169 1971 Size: 91 1078 802 Fragments arranged by size: 1078 802 91 NspI r_CATG'y Cuts at: 0 216 436 1971 Size: 216 220 1535 Fragments arranged by size: 1535 220 216 PasI CC'CwG_GG Cuts at: 0 975 1306 1338 1543 1971 Size: 975 331 32 205 428 Fragments arranged by size: 975 428 331 205 32 PciI A'CATG_T Cuts at: 0 212 1971 Size: 212 1759 PflMI CCAn_nnn'nTGG Cuts at: 0 194 1290 1338 1434 1971 Size: 194 1096 48 96 537 Fragments arranged by size: 1096 537 194 96 48 PfoI T'CCnGG_A Cuts at: 0 488 707 919 1971 Size: 488 219 212 1052 Fragments arranged by size: 1052 488 219 212 PpuMI rG'GwC_Cy Cuts at: 0 951 1066 1280 1302 1448 1582 1642 1702 Size: 951 115 214 22 146 134 60 60 Cuts at: 1702 1762 1822 1882 1971 Size: 60 60 60 89 Fragments arranged by size: 951 214 146 134 115 89 60 60 60 60 60 22 PspOMI G'GGCC_C Cuts at: 0 859 1030 1525 1971 Size: 859 171 495 446 Fragments arranged by size: 859 495 446 171 PspXI vC'TCGA_Gb Cuts at: 0 673 910 1971 Size: 673 237 1061 Fragments arranged by size: 1061 673 237 PstI C_TGCA'G Cuts at: 0 1590 1650 1770 1830 1971 Size: 1590 60 120 60 141 Fragments arranged by size: 1590 141 120 60 60 PvuII CAG'CTG Cuts at: 0 807 1971 Size: 807 1164 SacI G_AGCT'C Cuts at: 0 518 1971 Size: 518 1453 SacII CC_GC'GG Cuts at: 0 145 1971 Size: 145 1826 SanDI GG'GwC_CC Cuts at: 0 951 1066 1280 1448 1971 Size: 951 115 214 168 523 Fragments arranged by size: 951 523 214 168 115 SbfI CC_TGCA'GG Cuts at: 0 1590 1650 1770 1830 1971 Size: 1590 60 120 60 141 Fragments arranged by size: 1590 141 120 60 60 SfcI C'TryA_G Cuts at: 0 288 1586 1646 1766 1826 1971 Size: 288 1298 60 120 60 145 Fragments arranged by size: 1298 288 145 120 60 60 SfoI GGC'GCC Cuts at: 0 689 1971 Size: 689 1282 SgrAI Cr'CCGG_yG Cuts at: 0 91 1971 Size: 91 1880 SmaI CCC'GGG Cuts at: 0 948 1971 Size: 948 1023 SmlI C'TyrA_G Cuts at: 0 370 649 673 910 1128 1971 Size: 370 279 24 237 218 843 Fragments arranged by size: 843 370 279 237 218 24 SphI G_CATG'C Cuts at: 0 436 1971 Size: 436 1535 StyI C'CwwG_G Cuts at: 0 1433 1472 1904 1971 Size: 1433 39 432 67 Fragments arranged by size: 1433 432 67 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 566 649 988 1971 Size: 566 83 339 983 Fragments arranged by size: 983 566 339 83 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1334 1366 1971 Size: 1334 32 605 Fragments arranged by size: 1334 605 32 Tth111I GACn'n_nGTC Cuts at: 0 183 1971 Size: 183 1788 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 425 1971 Size: 425 1546 XhoI C'TCGA_G Cuts at: 0 673 910 1971 Size: 673 237 1061 Fragments arranged by size: 1061 673 237 XmaI C'CCGG_G Cuts at: 0 946 1971 Size: 946 1025 XmnI GAAnn'nnTTC Cuts at: 0 595 1971 Size: 595 1376 Enzymes that do cut: Acc65I AcuI AfeI AflII AflIII AhdI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BglI BlpI Bme1580I BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI DraI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI KpnI MmeI MscI MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI PfoI PpuMI PspOMI PspXI PstI PvuII SacI SacII SanDI SbfI SfcI SfoI SgrAI SmaI SmlI SphI StyI TaqII TstI Tth111I XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AccI AclI AgeI AleI AlfI AloI AscI AseI AsiSI AvrII BaeI BciVI BclI BfrBI BglII BmgBI BmrI BmtI BplI BsaAI BsaBI BsiEI BsiWI BsmI BspHI BsrGI BssHII BstBI BstEII BstZ17I BtgZI ClaI CspCI DraIII DrdI EagI EciI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI MfeI MluI MslI NcoI NdeI NheI NotI NruI NsiI PacI PmeI PmlI PpiI PshAI PsiI PsrI PvuI RsrII SalI SapI ScaI SexAI SfiI SgfI SnaBI SpeI SrfI SspI StuI SwaI TatI XbaI ZraI