(Circular) (Six-base) MAPSORT of: pFN21AB7984.seq Check: 9954 from: 1 to: 5677 pFN21AB7984 5677 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3487 278 Size: 53 83 186 936 1951 2468 Fragments arranged by size: 2468 1951 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3020 1937 Size: 1083 4594 Acc65I G'GTAC_C Cuts at: 2998 2998 Size: 5677 AclI AA'CG_TT Cuts at: 1563 3804 4177 1563 Size: 2241 373 3063 Fragments arranged by size: 3063 2241 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3740 5124 5392 1632 Size: 2108 1384 268 1917 Fragments arranged by size: 2108 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5480 AflIII A'CryG_T Cuts at: 1203 2358 2532 2850 2852 4576 5553 1203 Size: 1155 174 318 2 1724 977 1327 Fragments arranged by size: 1724 1327 1155 977 318 174 2 AgeI A'CCGG_T Cuts at: 4508 4508 Size: 5677 AhdI GACnn_n'nnGTC Cuts at: 4406 4406 Size: 5677 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5677 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5677 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2918 2950 2918 Size: 32 5645 AlwNI CAG_nnn'CTG Cuts at: 2323 3420 4992 2323 Size: 1097 1572 3008 Fragments arranged by size: 3008 1572 1097 ApaLI G'TGCA_C Cuts at: 2171 3734 4890 2171 Size: 1563 1156 2958 Fragments arranged by size: 2958 1563 1156 ApoI r'AATT_y Cuts at: 1493 2785 2986 3212 3364 5473 1493 Size: 1292 201 226 152 2109 1697 Fragments arranged by size: 2109 1697 1292 226 201 152 AseI AT'TA_AT Cuts at: 160 1032 4231 160 Size: 872 3199 1606 Fragments arranged by size: 3199 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5677 AvaI C'yCGr_G Cuts at: 1870 1943 2406 2728 2860 3002 1870 Size: 73 463 322 132 142 4545 Fragments arranged by size: 4545 463 322 142 132 73 BamHI G'GATC_C Cuts at: 3007 3374 3007 Size: 367 5310 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2249 2704 2794 2998 4453 Size: 325 217 627 462 455 90 204 1455 Cuts at: 4453 618 Size: 1842 Fragments arranged by size: 1842 1455 627 462 455 325 217 204 90 BanII G_rGCy'C Cuts at: 1391 2514 2735 2996 1391 Size: 1123 221 261 4072 Fragments arranged by size: 4072 1123 261 221 BbeI G_GCGC'C Cuts at: 2253 2253 Size: 5677 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4706 BbvCI CC'TCA_GC Cuts at: 2710 2710 Size: 5677 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3868 3902 1903 Size: 34 1931 34 3678 Fragments arranged by size: 3678 1931 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3569 4779 3569 Size: 1210 4467 BclI T'GATC_A Cuts at: 1548 1548 Size: 5677 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4288 136 Size: 107 122 71 631 605 2616 1525 Fragments arranged by size: 2616 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5672 5672 Size: 5677 BlpI GC'TnA_GC Cuts at: 3094 3094 Size: 5677 Bme1580I G_kGCm'C Cuts at: 1790 2175 2709 3738 4894 1790 Size: 385 534 1029 1156 2573 Fragments arranged by size: 2573 1156 1029 534 385 BmgBI CAC'GTC Cuts at: 1318 2245 1318 Size: 927 4750 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2569 3408 4366 Size: 416 53 83 399 24 1152 839 958 Cuts at: 4366 442 Size: 1753 Fragments arranged by size: 1753 1152 958 839 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5677 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2176 2186 2300 2345 2719 2884 4337 1239 Size: 937 10 114 45 374 165 1453 2579 Fragments arranged by size: 2579 1453 937 374 165 114 45 10 Bpu10I CC'TnA_GC Cuts at: 2710 2710 Size: 5677 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3802 4667 4965 3802 Size: 865 298 4514 Fragments arranged by size: 4514 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4340 882 Size: 617 2841 2219 Fragments arranged by size: 2841 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2853 3468 493 Size: 711 1649 615 2702 Fragments arranged by size: 2702 1649 711 615 BsaBI GATnn'nnATC Cuts at: 5671 5671 Size: 5677 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2250 2724 2801 3484 Size: 53 83 186 936 717 474 77 683 Cuts at: 3484 3866 4501 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 717 683 635 474 382 186 83 77 53 BsaWI w'CCGG_w Cuts at: 4110 4508 4782 4929 5340 4110 Size: 398 274 147 411 4447 Fragments arranged by size: 4447 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2165 2195 2918 2948 1178 Size: 30 165 30 762 30 723 30 3907 Fragments arranged by size: 3907 762 723 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2062 2140 2820 1186 Size: 876 78 680 4043 Fragments arranged by size: 4043 876 680 78 BseYI C'CCAG_C Cuts at: 2094 4880 5376 2094 Size: 2786 496 2395 Fragments arranged by size: 2786 2395 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2942 1163 Size: 573 1206 3898 Fragments arranged by size: 3898 1206 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2067 2141 3127 3888 4916 1540 Size: 275 186 66 74 986 761 1028 2301 Fragments arranged by size: 2301 1028 986 761 275 186 74 66 BsiHKAI G_wGCw'C Cuts at: 2040 2175 3738 3823 4894 2040 Size: 135 1563 85 1071 2823 Fragments arranged by size: 2823 1563 1071 135 85 BsmI GAATG_Cn' Cuts at: 3188 3281 3188 Size: 93 5584 BsmBI CGTCTCn'nnnn_ Cuts at: 2074 2074 Size: 5677 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 2175 2514 2709 2735 2996 3738 Size: 399 250 135 339 195 26 261 742 Cuts at: 3738 3823 4894 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 339 261 250 195 135 85 26 BspEI T'CCGG_A Cuts at: 5340 5340 Size: 5677 BspHI T'CATG_A Cuts at: 3566 5296 3566 Size: 1730 3947 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3032 844 Size: 2188 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3564 1394 Size: 429 1741 3507 Fragments arranged by size: 3507 1741 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4172 4346 65 Size: 1183 2924 174 1396 Fragments arranged by size: 2924 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2967 4321 4508 1700 Size: 110 1157 1354 187 2869 Fragments arranged by size: 2869 1354 1157 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5677 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5677 BssSI C'ACGA_G Cuts at: 3737 4749 3737 Size: 1012 4665 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5677 BstBI TT'CG_AA Cuts at: 4481 4481 Size: 5677 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5677 BstYI r'GATC_y Cuts at: 1972 3007 3374 3759 3776 5228 5314 5326 5672 Size: 1035 367 385 17 1452 86 12 346 Cuts at: 5672 1972 Size: 1977 Fragments arranged by size: 1977 1452 1035 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2106 2790 2973 513 Size: 552 1041 684 183 3217 Fragments arranged by size: 3217 1041 684 552 183 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4938 Fragments arranged by size: 4938 683 56 BtsI GCAGTG_nn' Cuts at: 2721 2967 3193 3987 4007 2721 Size: 246 226 794 20 4391 Fragments arranged by size: 4391 794 246 226 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5677 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5642 DraI TTT'AAA Cuts at: 2981 2981 Size: 5677 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2059 4684 809 Size: 507 743 2625 1802 Fragments arranged by size: 2625 1802 743 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2064 2868 3124 Size: 54 1425 50 275 18 234 804 256 Cuts at: 3124 4013 5642 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 804 275 256 234 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2064 3124 1537 Size: 275 252 1060 4090 Fragments arranged by size: 4090 1060 275 252 EarI CTCTTCn'nnn_ Cuts at: 1353 3607 1353 Size: 2254 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2572 4264 4638 4784 387 Size: 1064 138 983 1692 374 146 1280 Fragments arranged by size: 1692 1280 1064 983 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2176 2186 2300 2345 2719 2884 3740 Size: 393 544 10 114 45 374 165 856 Cuts at: 3740 4337 5124 5392 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 856 787 597 544 393 374 268 165 114 45 10 EcoO109I rG'GnC_Cy Cuts at: 2257 2572 2257 Size: 315 5362 EcoRI G'AATT_C Cuts at: 2785 2986 3364 2785 Size: 201 378 5098 Fragments arranged by size: 5098 378 201 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2042 2506 2042 Size: 464 5213 FspI TGC'GCA Cuts at: 4183 4183 Size: 5677 HaeII r_GCGC'y Cuts at: 1678 2253 4824 1678 Size: 575 2571 2531 Fragments arranged by size: 2571 2531 575 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2246 2278 4318 4350 4392 Size: 32 135 32 674 32 2040 32 42 Cuts at: 4392 4424 1373 Size: 32 2626 Fragments arranged by size: 2626 2040 674 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2632 3021 3267 669 Size: 988 281 694 389 246 3079 Fragments arranged by size: 3079 988 694 389 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5677 HpaI GTT'AAC Cuts at: 3267 3267 Size: 5677 KasI G'GCGC_C Cuts at: 2249 2249 Size: 5677 KpnI G_GTAC'C Cuts at: 3002 3002 Size: 5677 MfeI C'AATT_G Cuts at: 3276 3276 Size: 5677 MluI A'CGCG_T Cuts at: 5553 5553 Size: 5677 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2208 2961 4791 4975 1305 Size: 903 753 1830 184 2007 Fragments arranged by size: 2007 1830 903 753 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2870 10 Size: 54 1425 343 1038 2817 Fragments arranged by size: 2817 1425 1038 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2687 3635 3994 4153 518 Size: 648 1521 948 359 159 2042 Fragments arranged by size: 2042 1521 948 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2048 2290 2792 3093 3770 4918 5163 1606 Size: 442 242 502 301 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 502 442 301 245 242 NarI GG'CG_CC Cuts at: 2250 2250 Size: 5677 NcoI C'CATG_G Cuts at: 513 1065 2106 513 Size: 552 1041 4084 Fragments arranged by size: 4084 1041 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5677 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5677 NotI GC'GGCC_GC Cuts at: 3124 3124 Size: 5677 NspI r_CATG'y Cuts at: 1132 1229 2362 2536 2656 2692 3035 4580 1132 Size: 97 1133 174 120 36 343 1545 2229 Fragments arranged by size: 2229 1545 1133 343 174 120 97 36 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5677 PciI A'CATG_T Cuts at: 2358 2532 4576 2358 Size: 174 2044 3459 Fragments arranged by size: 3459 2044 174 PflMI CCAn_nnn'nTGG Cuts at: 1108 2323 1108 Size: 1215 4462 PfoI T'CCnGG_A Cuts at: 2316 2316 Size: 5677 PmeI GTTT'AAAC Cuts at: 2981 2981 Size: 5677 PmlI CAC'GTG Cuts at: 2853 2853 Size: 5677 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2918 2950 3746 3778 5259 5291 2918 Size: 32 796 32 1481 32 3304 Fragments arranged by size: 3304 1481 796 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2257 2257 Size: 5677 PshAI GACnn'nnGTC Cuts at: 1613 2723 1613 Size: 1110 4567 PsiI TTA'TAA Cuts at: 3247 3247 Size: 5677 PspXI vC'TCGA_Gb Cuts at: 2860 2860 Size: 5677 PstI C_TGCA'G Cuts at: 830 3029 830 Size: 2199 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5677 SacII CC_GC'GG Cuts at: 2793 2793 Size: 5677 SalI G'TCGA_C Cuts at: 1936 3019 1936 Size: 1083 4594 SbfI CC_TGCA'GG Cuts at: 3029 3029 Size: 5677 ScaI AGT'ACT Cuts at: 3925 5637 3925 Size: 1712 3965 SfcI C'TryA_G Cuts at: 826 1045 3025 4160 4841 5032 826 Size: 219 1980 1135 681 191 1471 Fragments arranged by size: 1980 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2251 2251 Size: 5677 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5677 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5677 SmaI CCC'GGG Cuts at: 1872 2730 3004 1872 Size: 858 274 4545 Fragments arranged by size: 4545 858 274 SmlI C'TyrA_G Cuts at: 820 1017 1943 2860 3781 4682 4944 820 Size: 197 926 917 921 901 262 1553 Fragments arranged by size: 1553 926 921 917 901 262 197 SnaBI TAC'GTA Cuts at: 493 3468 493 Size: 2975 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5677 SphI G_CATG'C Cuts at: 1132 3035 1132 Size: 1903 3774 SspI AAT'ATT Cuts at: 5 52 3601 5 Size: 47 3549 2081 Fragments arranged by size: 3549 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5677 StyI C'CwwG_G Cuts at: 513 1065 2106 2568 3116 513 Size: 552 1041 462 548 3074 Fragments arranged by size: 3074 1041 552 548 462 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2680 3704 3721 3874 4059 4504 325 Size: 1255 1100 1024 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1100 1024 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3923 5635 96 Size: 274 80 33 51 1443 1946 1712 138 Fragments arranged by size: 1946 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2165 2197 5637 5669 2165 Size: 32 3440 32 2173 Fragments arranged by size: 3440 2173 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5677 XbaI T'CTAG_A Cuts at: 3013 3013 Size: 5677 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2161 2161 Size: 5677 XhoI C'TCGA_G Cuts at: 1943 2860 1943 Size: 917 4760 XmaI C'CCGG_G Cuts at: 1870 2728 3002 1870 Size: 858 274 4545 Fragments arranged by size: 4545 858 274 XmnI GAAnn'nnTTC Cuts at: 2489 3806 2489 Size: 1317 4360 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3485 276 Size: 53 83 186 936 1951 2468 Fragments arranged by size: 2468 1951 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI