(Linear) (Six-base) MAPSORT of: KIEE4575.seq Check: 6751 from: 1 to: 2274 KIEE4575.orf With 182 enzymes: SgfI * July 3, 2012 19:01 .. AccI GT'mk_AC Cuts at: 0 353 1768 2274 Size: 353 1415 506 Fragments arranged by size: 1415 506 353 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 696 1351 2274 Size: 696 655 923 Fragments arranged by size: 923 696 655 AflIII A'CryG_T Cuts at: 0 1796 2274 Size: 1796 478 AleI CACnn'nnGTG Cuts at: 0 770 1582 2274 Size: 770 812 692 Fragments arranged by size: 812 770 692 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1678 1760 2274 Size: 1678 82 514 Fragments arranged by size: 1678 514 82 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 463 495 2274 Size: 463 32 1779 Fragments arranged by size: 1779 463 32 AlwNI CAG_nnn'CTG Cuts at: 0 1665 1920 2274 Size: 1665 255 354 Fragments arranged by size: 1665 354 255 ApaI G_GGCC'C Cuts at: 0 83 1948 2274 Size: 83 1865 326 Fragments arranged by size: 1865 326 83 ApaLI G'TGCA_C Cuts at: 0 763 1207 2274 Size: 763 444 1067 Fragments arranged by size: 1067 763 444 ApoI r'AATT_y Cuts at: 0 867 1623 2274 Size: 867 756 651 Fragments arranged by size: 867 756 651 BanII G_rGCy'C Cuts at: 0 83 254 1388 1948 2274 Size: 83 171 1134 560 326 Fragments arranged by size: 1134 560 326 171 83 BbsI GAAGACnn'nnnn_ Cuts at: 0 1581 1643 2274 Size: 1581 62 631 Fragments arranged by size: 1581 631 62 BbvCI CC'TCA_GC Cuts at: 0 1010 2274 Size: 1010 1264 BglI GCCn_nnn'nGGC Cuts at: 0 1259 2274 Size: 1259 1015 BglII A'GATC_T Cuts at: 0 617 1391 2274 Size: 617 774 883 Fragments arranged by size: 883 774 617 BlpI GC'TnA_GC Cuts at: 0 189 253 2274 Size: 189 64 2021 Fragments arranged by size: 2021 189 64 Bme1580I G_kGCm'C Cuts at: 0 83 378 587 767 1211 1948 2274 Size: 83 295 209 180 444 737 326 Fragments arranged by size: 737 444 326 295 209 180 83 BmrI ACTGGGnnnn_n' Cuts at: 0 388 675 777 1010 1276 2274 Size: 388 287 102 233 266 998 Fragments arranged by size: 998 388 287 266 233 102 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1135 1554 1852 1981 2158 2274 Size: 1135 419 298 129 177 116 Fragments arranged by size: 1135 419 298 177 129 116 Bpu10I CC'TnA_GC Cuts at: 0 1010 1351 1949 2274 Size: 1010 341 598 325 Fragments arranged by size: 1010 598 341 325 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 807 982 2193 2274 Size: 807 175 1211 81 Fragments arranged by size: 1211 807 175 81 BsaI GGTCTCn'nnnn_ Cuts at: 0 1416 2169 2274 Size: 1416 753 105 Fragments arranged by size: 1416 753 105 BsaAI yAC'GTr Cuts at: 0 582 1230 2274 Size: 582 648 1044 Fragments arranged by size: 1044 648 582 BsaHI Gr'CG_yC Cuts at: 0 1983 2274 Size: 1983 291 BsaWI w'CCGG_w Cuts at: 0 266 404 2274 Size: 266 138 1870 Fragments arranged by size: 1870 266 138 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 463 493 1201 1231 1979 2009 2274 Size: 463 30 708 30 748 30 265 Fragments arranged by size: 748 708 463 265 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 754 1053 2274 Size: 754 299 1221 Fragments arranged by size: 1221 754 299 BseYI C'CCAG_C Cuts at: 0 34 390 872 1083 2274 Size: 34 356 482 211 1191 Fragments arranged by size: 1191 482 356 211 34 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1486 1597 2274 Size: 1486 111 677 Fragments arranged by size: 1486 677 111 BsiHKAI G_wGCw'C Cuts at: 0 608 767 1211 2274 Size: 608 159 444 1063 Fragments arranged by size: 1063 608 444 159 BsmI GAATG_Cn' Cuts at: 0 564 579 2274 Size: 564 15 1695 Fragments arranged by size: 1695 564 15 Bsp1286I G_dGCh'C Cuts at: 0 83 254 378 587 608 767 1211 1388 Size: 83 171 124 209 21 159 444 177 Cuts at: 1388 1948 2274 Size: 560 326 Fragments arranged by size: 560 444 326 209 177 171 159 124 83 21 BsrBI CCG'CTC Cuts at: 0 246 1217 2274 Size: 246 971 1057 Fragments arranged by size: 1057 971 246 BsrDI GCAATG_nn' Cuts at: 0 635 1592 2274 Size: 635 957 682 Fragments arranged by size: 957 682 635 BsrFI r'CCGG_y Cuts at: 0 2012 2274 Size: 2012 262 BssSI C'ACGA_G Cuts at: 0 1451 2274 Size: 1451 823 BstBI TT'CG_AA Cuts at: 0 2109 2274 Size: 2109 165 BstEII G'GTnAC_C Cuts at: 0 153 1478 2274 Size: 153 1325 796 Fragments arranged by size: 1325 796 153 BstXI CCAn_nnnn'nTGG Cuts at: 0 338 2274 Size: 338 1936 BstYI r'GATC_y Cuts at: 0 617 826 1391 2274 Size: 617 209 565 883 Fragments arranged by size: 883 617 565 209 BstZ17I GTA'TAC Cuts at: 0 1769 2274 Size: 1769 505 BtgI C'CryG_G Cuts at: 0 609 683 2274 Size: 609 74 1591 Fragments arranged by size: 1591 609 74 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1320 2274 Size: 1320 954 BtsI GCAGTG_nn' Cuts at: 0 325 608 858 2274 Size: 325 283 250 1416 Fragments arranged by size: 1416 325 283 250 DrdI GACnn_nn'nnGTC Cuts at: 0 132 2274 Size: 132 2142 EaeI y'GGCC_r Cuts at: 0 612 911 1056 1251 2274 Size: 612 299 145 195 1023 Fragments arranged by size: 1023 612 299 195 145 EarI CTCTTCn'nnn_ Cuts at: 0 715 931 1721 2274 Size: 715 216 790 553 Fragments arranged by size: 790 715 553 216 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 159 2274 Size: 159 2115 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 696 1135 1351 1554 1852 1981 2158 2274 Size: 696 439 216 203 298 129 177 116 Fragments arranged by size: 696 439 298 216 203 177 129 116 EcoO109I rG'GnC_Cy Cuts at: 0 79 1894 1944 1945 2274 Size: 79 1815 50 1 329 Fragments arranged by size: 1815 329 79 50 1 EcoRI G'AATT_C Cuts at: 0 867 2274 Size: 867 1407 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 448 480 2274 Size: 448 32 1794 Fragments arranged by size: 1794 448 32 HincII GTy'rAC Cuts at: 0 1812 2274 Size: 1812 462 HindIII A'AGCT_T Cuts at: 0 186 2274 Size: 186 2088 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1904 2172 2274 Size: 1904 268 102 Fragments arranged by size: 1904 268 102 MscI TGG'CCA Cuts at: 0 614 913 1058 2274 Size: 614 299 145 1216 Fragments arranged by size: 1216 614 299 145 MslI CAynn'nnrTG Cuts at: 0 361 770 889 1204 1582 2274 Size: 361 409 119 315 378 692 Fragments arranged by size: 692 409 378 361 315 119 MspA1I CmG'CkG Cuts at: 0 796 1083 1662 2145 2274 Size: 796 287 579 483 129 Fragments arranged by size: 796 579 483 287 129 NaeI GCC'GGC Cuts at: 0 2014 2274 Size: 2014 260 NcoI C'CATG_G Cuts at: 0 683 2274 Size: 683 1591 NgoMIV G'CCGG_C Cuts at: 0 2012 2274 Size: 2012 262 NspI r_CATG'y Cuts at: 0 1329 1800 2274 Size: 1329 471 474 Fragments arranged by size: 1329 474 471 PasI CC'CwG_GG Cuts at: 0 1152 1282 2274 Size: 1152 130 992 Fragments arranged by size: 1152 992 130 PciI A'CATG_T Cuts at: 0 1796 2274 Size: 1796 478 PflMI CCAn_nnn'nTGG Cuts at: 0 1083 2274 Size: 1083 1191 PfoI T'CCnGG_A Cuts at: 0 452 1112 1997 2274 Size: 452 660 885 277 Fragments arranged by size: 885 660 452 277 PmlI CAC'GTG Cuts at: 0 582 1230 2274 Size: 582 648 1044 Fragments arranged by size: 1044 648 582 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 463 495 2274 Size: 463 32 1779 Fragments arranged by size: 1779 463 32 PspOMI G'GGCC_C Cuts at: 0 79 1944 2274 Size: 79 1865 330 Fragments arranged by size: 1865 330 79 PstI C_TGCA'G Cuts at: 0 1670 1916 2274 Size: 1670 246 358 Fragments arranged by size: 1670 358 246 PvuII CAG'CTG Cuts at: 0 796 1083 1662 2145 2274 Size: 796 287 579 483 129 Fragments arranged by size: 796 579 483 287 129 SapI GCTCTTCn'nnn_ Cuts at: 0 715 2274 Size: 715 1559 SfcI C'TryA_G Cuts at: 0 1666 1817 1912 2274 Size: 1666 151 95 362 Fragments arranged by size: 1666 362 151 95 SgrAI Cr'CCGG_yG Cuts at: 0 2012 2274 Size: 2012 262 SmlI C'TyrA_G Cuts at: 0 822 997 2172 2274 Size: 822 175 1175 102 Fragments arranged by size: 1175 822 175 102 SspI AAT'ATT Cuts at: 0 691 2274 Size: 691 1583 StyI C'CwwG_G Cuts at: 0 683 2274 Size: 683 1591 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1165 1491 2274 Size: 1165 326 783 Fragments arranged by size: 1165 783 326 TatI w'GTAC_w Cuts at: 0 48 629 2274 Size: 48 581 1645 Fragments arranged by size: 1645 581 48 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 399 431 1201 1233 2274 Size: 399 32 770 32 1041 Fragments arranged by size: 1041 770 399 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 366 2274 Size: 366 1908 Enzymes that do cut: AccI AcuI AflIII AleI AlfI AloI AlwNI ApaI ApaLI ApoI BanII BbsI BbvCI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BsrBI BsrDI BsrFI BssSI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI DrdI EaeI EarI EciI Eco57MI EcoO109I EcoRI Hin4I HincII HindIII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmlI PpiI PspOMI PstI PvuII SapI SfcI SgrAI SmlI SspI StyI TaqII TatI TstI XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BcgI BciVI BclI BfrBI BmgBI BmtI BplI BsaBI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrGI BssHII BstAPI Bsu36I ClaI CspCI DraI DraIII EagI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HaeII HpaI KasI KpnI MfeI MluI NarI NdeI NheI NotI NruI NsiI PacI PmeI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SmaI SnaBI SpeI SphI SrfI StuI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI