################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI AspEI AspI AviII AvrII BalI BbeI BbrPI BcgI BciVI BfrI BglI BlnI BsaAI BsaBI BsaHI BsaI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI Hin1I HindIII KasI KspI MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI StuI SwaI Tsp45I Tsp509I Tth111I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### Acc65I 434 GGTACC G^GTACC Alw44I 421 GTGCAC G^TGCAC ApaI 1112 GGGCCC GGGCC^C ApaLI 421 GTGCAC G^TGCAC Asp700I 297 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 434 GGTACC G^GTACC BclI 90 TGATCA T^GATCA BglII 341 AGATCT A^GATCT BlpI 1599 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1599 GCTGAGC GC^T[ACGT]AGC BsaWI 509 ACCGGA [AT]^CCGG[AT] BsrDI 1535 GCAATG GCAATG(2/0) BsrFI 1437 GCCGGT [GA]^CCGG[CT] BstAPI 1014 GCAGATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 1599 GCTGAGC GC^T[ACGT]AGC Cfr10I 1437 GCCGGT [GA]^CCGG[CT] EcoNI 1290 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 90 TGATCA T^GATCA HaeII 1160 AGCGCC [GA]GCGC^[CT] HincII 699 GTTAAC GT[CT]^[GA]AC HindII 699 GTTAAC GT[CT]^[GA]AC HpaI 699 GTTAAC GTT^AAC KpnI 434 GGTACC GGTAC^C MaeII 816 ACGT A^CGT MaeIII 25 GTTAC ^GT[ACGT]AC MslI 897 CATCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 32 CCATGG C^CATGG NspI 525 GCATGC [GA]CATG^[CT] SmaI 926 CCCGGG CCC^GGG SphI 525 GCATGC GCATG^C SspI 676 AATATT AAT^ATT XmaCI 926 CCCGGG C^CCGGG XmaI 926 CCCGGG C^CCGGG XmnI 297 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AlwNI 586 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG 1588 CAGCTGCTG AvaI 926 CCCGGG C^[CT]CG[GA]G 1622 CCCGAG BamHI 512 GGATCC G^GATCC 836 GGATCC BanI 434 GGTACC G^G[CT][GA]CC 789 GGCACC BbsI 1068 GAAGAC GAAGAC(2/6) 1371 GAAGAC BbvCI 438 CCTCAGC CCTCAGC(-5/-2) 268 GCTGAGG BfaI 1165 CTAG C^TAG 1511 CTAG BpuAI 1068 GAAGAC GAAGAC(2/6) 1371 GAAGAC BsmAI 358 GAGAC GTCTC(1/5) 1212 GAGAC BsoBI 926 CCCGGG C^[CT]CG[GA]G 1622 CCCGAG BsrBI 448 GAGCGG CCGCTC(-3/-3) 955 GAGCGG DraIII 142 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG 1390 CACCTGGTG EarI 1294 GAAGAG CTCTTC(1/4) 1327 GAAGAG HgaI 1596 GACGC GACGC(5/10) 1698 GACGC Ksp632I 1294 GAAGAG CTCTTC(1/4) 1327 GAAGAG MaeI 1165 CTAG C^TAG 1511 CTAG MseI 700 TTAA T^TAA 770 TTAA PflMI 141 CCACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 706 CCAAAGATTGG SacI 274 GAGCTC GAGCT^C 1090 GAGCTC SexAI 1364 ACCTGGT A^CC[AT]GGT 1391 ACCTGGT Tru9I 700 TTAA T^TAA 770 TTAA Van91I 141 CCACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 706 CCAAAGATTGG ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AspHI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI TseI TspRI TthHB8I XhoII