################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccI AclI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BseRI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FseI FspI HaeII HincII HindII HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MflI MluI MluNI MscI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccIII 589 TCCGGA T^CCGGA AcsI 421 GAATTC [GA]^AATT[CT] AcyI 6 GGCGTC G[GA]^CG[CT]C ApaI 41 GGGCCC GGGCC^C ApoI 421 GAATTC [GA]^AATT[CT] AvaII 117 GGACC G^G[AT]CC BanI 61 GGCACC G^G[CT][GA]CC BbsI 529 GAAGAC GAAGAC(2/6) BclI 32 TGATCA T^GATCA BfaI 304 CTAG C^TAG BpmI 108 CTCCAG CTGGAG(16/14) BpuAI 529 GAAGAC GAAGAC(2/6) BsaHI 6 GGCGTC G[GA]^CG[CT]C BsaWI 589 TCCGGA [AT]^CCGG[AT] BseAI 589 TCCGGA T^CCGGA BsmBI 499 GAGACG CGTCTC(1/5) BspEI 589 TCCGGA T^CCGGA BspMI 119 ACCTGC ACCTGC(4/8) BsrBI 26 GAGCGG CCGCTC(-3/-3) BstNI 549 CCTGG CC^[AT]GG BstXI 434 CCAAAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EaeI 402 TGGCCG [CT]^GGCC[GA] EcoRI 421 GAATTC G^AATTC EcoRII 549 CCTGG ^CC[AT]GG FbaI 32 TGATCA T^GATCA HgaI 7 GCGTC GACGC(5/10) Hin1I 6 GGCGTC G[GA]^CG[CT]C HindIII 665 AAGCTT A^AGCTT MaeI 304 CTAG C^TAG MaeIII 673 GTTAC ^GT[ACGT]AC MboII 529 GAAGA GAAGA(8/7) MroI 589 TCCGGA T^CCGGA MspA1I 111 CAGCGG C[AC]G^C[GT]G MvaI 549 CCTGG CC^[AT]GG MwoI 15 GCGGCTGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NspI 134 ACATGC [GA]CATG^[CT] PpuMI 116 GGGACCT [GA]G^G[AT]CC[CT] SfaNI 77 GCATC GCATC(5/9) SmaI 45 CCCGGG CCC^GGG SspI 554 AATATT AAT^ATT TfiI 387 GATTC G^A[AT]TC XmaCI 45 CCCGGG C^CCGGG XmaI 45 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AvaI 45 CCCGGG C^[CT]CG[GA]G 51 CTCGGG BanII 41 GGGCCC G[GA]GC[CT]^C 48 GGGCTC BsmAI 499 GAGAC GTCTC(1/5) 513 GAGAC BsmFI 116 GGGAC GGGAC(10/14) 128 GGGAC BsoBI 45 CCCGGG C^[CT]CG[GA]G 51 CTCGGG BspHI 209 TCATGA T^CATGA 228 TCATGA CfoI 13 GCGC GCG^C 75 GCGC DpnII 33 GATC ^GATC 321 GATC HhaI 13 GCGC GCG^C 75 GCGC HinP1I 13 GCGC G^CGC 75 GCGC HphI 30 GGTGA GGTGA(8/7) 245 TCACC MboI 33 GATC ^GATC 321 GATC MseI 335 TTAA T^TAA 362 TTAA NdeII 33 GATC ^GATC 321 GATC PleI 213 GACTC GAGTC(4/5) 407 GAGTC RcaI 209 TCATGA T^CATGA 228 TCATGA Sau3AI 33 GATC ^GATC 321 GATC TaqI 92 TCGA T^CGA 323 TCGA Tru9I 335 TTAA T^TAA 362 TTAA Tsp509I 153 AATT ^AATT 422 AATT TspRI 329 CAGTG CA[GC]TG[ACGT][ACGT]^ 399 CAGTG TthHB8I 92 TCGA T^CGA 323 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AccII AciI AluI BbvI BcnI BmyI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstUI Cac8I Cfr13I DraII EcoO109I EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MnlI MspI MvnI NciI NlaIII NlaIV Sau96I ScrFI StyI TseI