(Circular) (Six-base) MAPSORT of: pFN21AE6420.seq Check: 4075 from: 1 to: 6226 pFN21AE6420 6226 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2730 4036 278 Size: 53 83 186 936 1194 1306 2468 Fragments arranged by size: 2468 1306 1194 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2175 2892 3569 1937 Size: 238 717 677 4594 Fragments arranged by size: 4594 717 677 238 Acc65I G'GTAC_C Cuts at: 3547 3547 Size: 6226 AclI AA'CG_TT Cuts at: 1563 4353 4726 1563 Size: 2790 373 3063 Fragments arranged by size: 3063 2790 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4289 5673 5941 1632 Size: 2657 1384 268 1917 Fragments arranged by size: 2657 1917 1384 268 AfeI AGC'GCT Cuts at: 2207 2207 Size: 6226 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6029 AflIII A'CryG_T Cuts at: 1203 2472 2817 5125 6102 1203 Size: 1269 345 2308 977 1327 Fragments arranged by size: 2308 1327 1269 977 345 AgeI A'CCGG_T Cuts at: 5057 5057 Size: 6226 AhdI GACnn_n'nnGTC Cuts at: 4955 4955 Size: 6226 AleI CACnn'nnGTG Cuts at: 1166 3191 1166 Size: 2025 4201 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3041 1184 Size: 1857 4369 AlwNI CAG_nnn'CTG Cuts at: 2866 3163 3969 5541 2866 Size: 297 806 1572 3551 Fragments arranged by size: 3551 1572 806 297 ApaI G_GGCC'C Cuts at: 2036 2812 2036 Size: 776 5450 ApaLI G'TGCA_C Cuts at: 4283 5439 4283 Size: 1156 5070 ApoI r'AATT_y Cuts at: 1493 3535 3761 3913 6022 1493 Size: 2042 226 152 2109 1697 Fragments arranged by size: 2109 2042 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4780 160 Size: 872 3748 1606 Fragments arranged by size: 3748 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6226 AvaI C'yCGr_G Cuts at: 1870 1943 2054 2673 3455 3551 1870 Size: 73 111 619 782 96 4545 Fragments arranged by size: 4545 782 619 111 96 73 BamHI G'GATC_C Cuts at: 2215 2543 3556 3923 2215 Size: 328 1013 367 4518 Fragments arranged by size: 4518 1013 367 328 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2608 3193 3391 3547 5002 Size: 325 217 627 821 585 198 156 1455 Cuts at: 5002 618 Size: 1842 Fragments arranged by size: 1842 1455 821 627 585 325 217 198 156 BanII G_rGCy'C Cuts at: 1391 2036 2448 2509 2812 3110 3312 3545 1391 Size: 645 412 61 303 298 202 233 4072 Fragments arranged by size: 4072 645 412 303 298 233 202 61 BbeI G_GCGC'C Cuts at: 2612 2612 Size: 6226 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5255 BbvCI CC'TCA_GC Cuts at: 2799 2799 Size: 6226 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4417 4451 1903 Size: 34 50 34 2396 34 3678 Fragments arranged by size: 3678 2396 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4118 5328 4118 Size: 1210 5016 BclI T'GATC_A Cuts at: 1548 1548 Size: 6226 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2606 2699 4837 Size: 107 122 71 631 605 934 93 2138 Cuts at: 4837 136 Size: 1525 Fragments arranged by size: 2138 1525 934 631 605 122 107 93 71 BglII A'GATC_T Cuts at: 6221 6221 Size: 6226 BlpI GC'TnA_GC Cuts at: 2333 2636 3643 2333 Size: 303 1007 4916 Fragments arranged by size: 4916 1007 303 Bme1580I G_kGCm'C Cuts at: 1790 2036 2812 3198 3292 3456 4287 5443 1790 Size: 246 776 386 94 164 831 1156 2573 Fragments arranged by size: 2573 1156 831 776 386 246 164 94 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6226 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2749 3957 4915 Size: 416 53 83 399 24 1332 1208 958 Cuts at: 4915 442 Size: 1753 Fragments arranged by size: 1753 1332 1208 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6226 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2222 3371 3497 4886 1239 Size: 983 1149 126 1389 2579 Fragments arranged by size: 2579 1389 1149 983 126 Bpu10I CC'TnA_GC Cuts at: 2799 2799 Size: 6226 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4351 5216 5514 4351 Size: 865 298 5063 Fragments arranged by size: 5063 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3452 4889 882 Size: 617 1953 1437 2219 Fragments arranged by size: 2219 1953 1437 617 BsaAI yAC'GTr Cuts at: 493 1204 2473 2818 4017 493 Size: 711 1269 345 1199 2702 Fragments arranged by size: 2702 1269 1199 711 345 BsaBI GATnn'nnATC Cuts at: 6220 6220 Size: 6226 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2609 2727 4033 4415 Size: 53 83 186 936 1076 118 1306 382 Cuts at: 4415 5050 275 Size: 635 1451 Fragments arranged by size: 1451 1306 1076 936 635 382 186 118 83 53 BsaWI w'CCGG_w Cuts at: 2769 4659 5057 5331 5478 5889 2769 Size: 1890 398 274 147 411 3106 Fragments arranged by size: 3106 1890 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6001 Fragments arranged by size: 6001 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6226 BseYI C'CCAG_C Cuts at: 2020 2194 2523 3240 5429 5925 2020 Size: 174 329 717 2189 496 2321 Fragments arranged by size: 2321 2189 717 496 329 174 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5653 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2286 2367 3676 4437 5465 1540 Size: 275 186 285 81 1309 761 1028 2301 Fragments arranged by size: 2301 1309 1028 761 285 275 186 81 BsiHKAI G_wGCw'C Cuts at: 2145 2619 3377 4287 4372 5443 2145 Size: 474 758 910 85 1071 2928 Fragments arranged by size: 2928 1071 910 758 474 85 BsmI GAATG_Cn' Cuts at: 3737 3830 3737 Size: 93 6133 BsmBI CGTCTCn'nnnn_ Cuts at: 3040 3040 Size: 6226 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 2145 2448 2509 2619 2812 3110 Size: 399 246 109 303 61 110 193 298 Cuts at: 3110 3198 3292 3312 3377 3456 3545 4287 4372 Size: 88 94 20 65 79 89 742 85 Cuts at: 4372 5443 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 303 298 246 193 110 109 94 89 88 85 79 65 61 20 BspEI T'CCGG_A Cuts at: 2769 5889 2769 Size: 3120 3106 BspHI T'CATG_A Cuts at: 2622 4115 5845 2622 Size: 1493 1730 3003 Fragments arranged by size: 3003 1730 1493 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2232 2904 3581 844 Size: 1388 672 677 3489 Fragments arranged by size: 3489 1388 677 672 BsrBI CCG'CTC Cuts at: 1394 1823 4113 1394 Size: 429 2290 3507 Fragments arranged by size: 3507 2290 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4721 4895 65 Size: 1183 3473 174 1396 Fragments arranged by size: 3473 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2366 2823 4870 5057 1700 Size: 110 556 457 2047 187 2869 Fragments arranged by size: 2869 2047 556 457 187 110 BsrGI T'GTAC_A Cuts at: 96 3078 96 Size: 2982 3244 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6226 BssSI C'ACGA_G Cuts at: 4286 5298 4286 Size: 1012 5214 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6226 BstBI TT'CG_AA Cuts at: 5030 5030 Size: 6226 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6226 BstXI CCAn_nnnn'nTGG Cuts at: 2836 3421 2836 Size: 585 5641 BstYI r'GATC_y Cuts at: 1972 2215 2543 2754 3556 3923 4308 4325 5777 Size: 243 328 211 802 367 385 17 1452 Cuts at: 5777 5863 5875 6221 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 802 385 367 346 328 243 211 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3304 3304 Size: 6226 BtgI C'CryG_G Cuts at: 513 1065 2579 2835 3000 3234 3292 513 Size: 552 1514 256 165 234 58 3447 Fragments arranged by size: 3447 1514 552 256 234 165 58 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5487 Fragments arranged by size: 5487 683 56 BtsI GCAGTG_nn' Cuts at: 2145 2541 3117 3742 4536 4556 2145 Size: 396 576 625 794 20 3815 Fragments arranged by size: 3815 794 625 576 396 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6226 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6191 DraI TTT'AAA Cuts at: 3530 3530 Size: 6226 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2732 5233 809 Size: 507 1416 2501 1802 Fragments arranged by size: 2501 1802 1416 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2124 2283 2364 Size: 54 1425 50 275 18 294 159 81 Cuts at: 2364 2598 3042 3204 3321 3411 3673 4562 6191 Size: 234 444 162 117 90 262 889 1629 Cuts at: 6191 8 Size: 43 Fragments arranged by size: 1629 1425 889 444 294 275 262 234 162 159 117 90 81 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2283 2364 3673 1537 Size: 275 471 81 1309 4090 Fragments arranged by size: 4090 1309 471 275 81 EarI CTCTTCn'nnn_ Cuts at: 1353 2682 3035 3221 4156 1353 Size: 1329 353 186 935 3423 Fragments arranged by size: 3423 1329 935 353 186 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4813 5187 5333 387 Size: 1064 138 3224 374 146 1280 Fragments arranged by size: 3224 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2222 3371 3497 4289 4886 5673 5941 Size: 393 590 1149 126 792 597 787 268 Cuts at: 5941 1239 Size: 1524 Fragments arranged by size: 1524 1149 792 787 597 590 393 268 126 EcoO109I rG'GnC_Cy Cuts at: 2032 2097 2692 2809 3245 3274 3513 2032 Size: 65 595 117 436 29 239 4745 Fragments arranged by size: 4745 595 436 239 117 65 29 EcoRI G'AATT_C Cuts at: 3535 3913 3535 Size: 378 5848 FspI TGC'GCA Cuts at: 2391 4732 2391 Size: 2341 3885 FspAI rTGC'GCAy Cuts at: 2391 2391 Size: 6226 HaeII r_GCGC'y Cuts at: 1678 2209 2587 2612 2705 5373 1678 Size: 531 378 25 93 2668 2531 Fragments arranged by size: 2668 2531 531 378 93 25 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4867 4899 4941 4973 1373 Size: 32 135 32 3295 32 42 32 2626 Fragments arranged by size: 3295 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3570 3816 669 Size: 988 281 1632 246 3079 Fragments arranged by size: 3079 1632 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6226 HpaI GTT'AAC Cuts at: 3816 3816 Size: 6226 KasI G'GCGC_C Cuts at: 2608 2608 Size: 6226 KpnI G_GTAC'C Cuts at: 3551 3551 Size: 6226 MfeI C'AATT_G Cuts at: 3825 3825 Size: 6226 MluI A'CGCG_T Cuts at: 6102 6102 Size: 6226 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5340 5524 1305 Size: 4035 184 2007 Fragments arranged by size: 4035 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2126 3206 3323 10 Size: 54 1425 343 294 1080 117 2913 Fragments arranged by size: 2913 1425 1080 343 294 117 54 MslI CAynn'nnrTG Cuts at: 518 1166 2465 2534 2834 3191 4184 4543 4702 Size: 648 1299 69 300 357 993 359 159 Cuts at: 4702 518 Size: 2042 Fragments arranged by size: 2042 1299 993 648 359 357 300 159 69 MspA1I CmG'CkG Cuts at: 1606 2017 2282 2351 2453 2866 2921 3041 3176 Size: 411 265 69 102 413 55 120 135 Cuts at: 3176 3327 3642 4319 5467 5712 1606 Size: 151 315 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 413 411 315 265 245 151 135 120 102 69 55 NaeI GCC'GGC Cuts at: 2368 2825 2368 Size: 457 5769 NarI GG'CG_CC Cuts at: 2609 2609 Size: 6226 NcoI C'CATG_G Cuts at: 513 1065 3000 513 Size: 552 1935 3739 Fragments arranged by size: 3739 1935 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6226 NgoMIV G'CCGG_C Cuts at: 2366 2823 2366 Size: 457 5769 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6226 NotI GC'GGCC_GC Cuts at: 3673 3673 Size: 6226 NspI r_CATG'y Cuts at: 1132 1229 3584 5129 1132 Size: 97 2355 1545 2229 Fragments arranged by size: 2355 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2272 1348 Size: 924 5302 PciI A'CATG_T Cuts at: 5125 5125 Size: 6226 PflMI CCAn_nnn'nTGG Cuts at: 1108 2530 3255 1108 Size: 1422 725 4079 Fragments arranged by size: 4079 1422 725 PfoI T'CCnGG_A Cuts at: 2757 2757 Size: 6226 PmeI GTTT'AAAC Cuts at: 3530 3530 Size: 6226 PmlI CAC'GTG Cuts at: 2818 2818 Size: 6226 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4295 4327 5808 5840 4295 Size: 32 1481 32 4681 Fragments arranged by size: 4681 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2097 3245 3274 3513 2097 Size: 1148 29 239 4810 Fragments arranged by size: 4810 1148 239 29 PshAI GACnn'nnGTC Cuts at: 1613 2726 1613 Size: 1113 5113 PsiI TTA'TAA Cuts at: 3796 3796 Size: 6226 PspOMI G'GGCC_C Cuts at: 2032 2808 2032 Size: 776 5450 PstI C_TGCA'G Cuts at: 830 2106 2862 2901 3578 830 Size: 1276 756 39 677 3478 Fragments arranged by size: 3478 1276 756 677 39 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6226 PvuII CAG'CTG Cuts at: 2351 2453 2866 3327 2351 Size: 102 413 461 5250 Fragments arranged by size: 5250 461 413 102 SalI G'TCGA_C Cuts at: 1936 3568 1936 Size: 1632 4594 SanDI GG'GwC_CC Cuts at: 2097 3245 2097 Size: 1148 5078 SapI GCTCTTCn'nnn_ Cuts at: 3035 3221 3035 Size: 186 6040 SbfI CC_TGCA'GG Cuts at: 3578 3578 Size: 6226 ScaI AGT'ACT Cuts at: 4474 6186 4474 Size: 1712 4514 SfcI C'TryA_G Cuts at: 826 1045 2102 2858 2897 3574 4709 5390 5581 Size: 219 1057 756 39 677 1135 681 191 Cuts at: 5581 826 Size: 1471 Fragments arranged by size: 1471 1135 1057 756 681 677 219 191 39 SfoI GGC'GCC Cuts at: 2610 2610 Size: 6226 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6226 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6226 SmaI CCC'GGG Cuts at: 1872 3553 1872 Size: 1681 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4330 5231 5493 820 Size: 197 926 2387 901 262 1553 Fragments arranged by size: 2387 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4017 493 Size: 3524 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6226 SphI G_CATG'C Cuts at: 1132 3584 1132 Size: 2452 3774 SspI AAT'ATT Cuts at: 5 52 4150 5 Size: 47 4098 2081 Fragments arranged by size: 4098 2081 47 StuI AGG'CCT Cuts at: 1517 2292 3100 1517 Size: 775 808 4643 Fragments arranged by size: 4643 808 775 StyI C'CwwG_G Cuts at: 513 1065 2211 2563 2937 3000 3462 3665 513 Size: 552 1146 352 374 63 462 203 3074 Fragments arranged by size: 3074 1146 552 462 374 352 203 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4253 4270 4423 4608 5053 325 Size: 1255 2673 17 153 185 445 1498 Fragments arranged by size: 2673 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3078 4472 6184 Size: 274 80 33 51 1443 1101 1394 1712 Cuts at: 6184 96 Size: 138 Fragments arranged by size: 1712 1443 1394 1101 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6186 6218 6186 Size: 32 6194 Tth111I GACn'n_nGTC Cuts at: 1316 2765 1316 Size: 1449 4777 XbaI T'CTAG_A Cuts at: 3562 3562 Size: 6226 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3067 3067 Size: 6226 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6226 XmaI C'CCGG_G Cuts at: 1870 3551 1870 Size: 1681 4545 XmnI GAAnn'nnTTC Cuts at: 4355 4355 Size: 6226 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2728 4034 276 Size: 53 83 186 936 1194 1306 2468 Fragments arranged by size: 2468 1306 1194 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI NruI NsiI PacI PspXI PsrI RsrII SacI SacII SexAI SfiI SrfI SwaI