(Circular) (Six-base) MAPSORT of: pFN21AE1545.seq Check: 8190 from: 1 to: 5926 pFN21AE1545 5926 bp With 182 enzymes: SgfI * August 16, 2010 14:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3736 278 Size: 53 83 186 936 2200 2468 Fragments arranged by size: 2468 2200 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3269 1937 Size: 1332 4594 Acc65I G'GTAC_C Cuts at: 3247 3247 Size: 5926 AclI AA'CG_TT Cuts at: 1563 4053 4426 1563 Size: 2490 373 3063 Fragments arranged by size: 3063 2490 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2475 3159 3989 5373 5641 1632 Size: 843 684 830 1384 268 1917 Fragments arranged by size: 1917 1384 843 830 684 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5729 AflIII A'CryG_T Cuts at: 1203 4825 5802 1203 Size: 3622 977 1327 Fragments arranged by size: 3622 1327 977 AgeI A'CCGG_T Cuts at: 4757 4757 Size: 5926 AhdI GACnn_n'nnGTC Cuts at: 4655 4655 Size: 5926 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5926 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5926 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2282 2314 2409 2441 2282 Size: 32 95 32 5767 Fragments arranged by size: 5767 95 32 32 AlwNI CAG_nnn'CTG Cuts at: 2246 2906 3669 5241 2246 Size: 660 763 1572 2931 Fragments arranged by size: 2931 1572 763 660 ApaLI G'TGCA_C Cuts at: 3983 5139 3983 Size: 1156 4770 ApoI r'AATT_y Cuts at: 1493 2119 2684 2706 2957 3235 3461 3613 5722 Size: 626 565 22 251 278 226 152 2109 Cuts at: 5722 1493 Size: 1697 Fragments arranged by size: 2109 1697 626 565 278 251 226 152 22 AseI AT'TA_AT Cuts at: 160 1032 2760 4480 160 Size: 872 1728 1720 1606 Fragments arranged by size: 1728 1720 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5926 AvaI C'yCGr_G Cuts at: 1870 1943 2483 3251 1870 Size: 73 540 768 4545 Fragments arranged by size: 4545 768 540 73 BamHI G'GATC_C Cuts at: 3256 3623 3256 Size: 367 5559 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3247 4702 618 Size: 325 217 627 1460 1455 1842 Fragments arranged by size: 1842 1460 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3245 1391 Size: 1854 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3150 928 Size: 971 1251 3704 Fragments arranged by size: 3704 1251 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4117 4151 1903 Size: 34 2180 34 3678 Fragments arranged by size: 3678 2180 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2782 3818 5028 2782 Size: 1036 1210 3680 Fragments arranged by size: 3680 1210 1036 BclI T'GATC_A Cuts at: 1548 2985 1548 Size: 1437 4489 BfrBI ATG'CAT Cuts at: 2884 2884 Size: 5926 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4537 136 Size: 107 122 71 631 605 2865 1525 Fragments arranged by size: 2865 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5921 5921 Size: 5926 BlpI GC'TnA_GC Cuts at: 3343 3343 Size: 5926 Bme1580I G_kGCm'C Cuts at: 1790 3987 5143 1790 Size: 2197 1156 2573 Fragments arranged by size: 2573 2197 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5926 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3657 4615 442 Size: 416 53 83 399 24 2240 958 1753 Fragments arranged by size: 2240 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5926 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4586 1239 Size: 3347 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2214 4051 4916 5214 2214 Size: 1837 865 298 2926 Fragments arranged by size: 2926 1837 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2300 4589 882 Size: 617 801 2289 2219 Fragments arranged by size: 2289 2219 801 617 BsaAI yAC'GTr Cuts at: 493 1204 3717 493 Size: 711 2513 2702 Fragments arranged by size: 2702 2513 711 BsaBI GATnn'nnATC Cuts at: 5920 5920 Size: 5926 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3733 4115 4750 275 Size: 53 83 186 936 2200 382 635 1451 Fragments arranged by size: 2200 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2961 4359 4757 5031 5178 5589 2961 Size: 1398 398 274 147 411 3298 Fragments arranged by size: 3298 1398 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2282 2312 3100 3130 1178 Size: 30 165 30 879 30 788 30 3974 Fragments arranged by size: 3974 879 788 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3209 1186 Size: 2023 3903 BseYI C'CCAG_C Cuts at: 5129 5625 5129 Size: 496 5430 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5353 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3376 4137 5165 1540 Size: 275 186 1375 761 1028 2301 Fragments arranged by size: 2301 1375 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3987 4072 5143 3987 Size: 85 1071 4770 Fragments arranged by size: 4770 1071 85 BsmI GAATG_Cn' Cuts at: 3437 3530 3437 Size: 93 5833 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3245 3987 4072 5143 1391 Size: 399 1455 742 85 1071 2174 Fragments arranged by size: 2174 1455 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5589 5589 Size: 5926 BspHI T'CATG_A Cuts at: 2688 2949 3815 5545 2688 Size: 261 866 1730 3069 Fragments arranged by size: 3069 1730 866 261 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3281 844 Size: 2437 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3813 1394 Size: 429 1990 3507 Fragments arranged by size: 3507 1990 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4421 4595 65 Size: 1183 3173 174 1396 Fragments arranged by size: 3173 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4570 4757 1700 Size: 110 2760 187 2869 Fragments arranged by size: 2869 2760 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5926 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5926 BssSI C'ACGA_G Cuts at: 3986 4998 3986 Size: 1012 4914 BstAPI GCAn_nnn'nTGC Cuts at: 794 2446 794 Size: 1652 4274 BstBI TT'CG_AA Cuts at: 2428 2704 4730 2428 Size: 276 2026 3624 Fragments arranged by size: 3624 2026 276 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5926 BstXI CCAn_nnnn'nTGG Cuts at: 2341 3211 2341 Size: 870 5056 BstYI r'GATC_y Cuts at: 1972 2352 2695 3256 3623 4008 4025 5477 5563 Size: 380 343 561 367 385 17 1452 86 Cuts at: 5563 5575 5921 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 561 385 380 367 346 343 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2019 2575 513 Size: 552 954 556 3864 Fragments arranged by size: 3864 954 556 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5187 Fragments arranged by size: 5187 683 56 BtsI GCAGTG_nn' Cuts at: 3442 4236 4256 3442 Size: 794 20 5112 Fragments arranged by size: 5112 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5926 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5891 DraI TTT'AAA Cuts at: 2124 3230 2124 Size: 1106 4820 DraIII CAC_nnn'GTG Cuts at: 2798 2798 Size: 5926 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4933 809 Size: 507 3617 1802 Fragments arranged by size: 3617 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3373 4262 5891 Size: 54 1425 50 275 18 1543 889 1629 Cuts at: 5891 8 Size: 43 Fragments arranged by size: 1629 1543 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3373 1537 Size: 275 1561 4090 Fragments arranged by size: 4090 1561 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3856 1353 Size: 2503 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4513 4887 5033 387 Size: 1064 138 2924 374 146 1280 Fragments arranged by size: 2924 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2475 3159 3989 4586 5373 5641 1239 Size: 393 843 684 830 597 787 268 1524 Fragments arranged by size: 1524 843 830 787 684 597 393 268 EcoRI G'AATT_C Cuts at: 2706 2957 3235 3613 2706 Size: 251 278 378 5019 Fragments arranged by size: 5019 378 278 251 FspI TGC'GCA Cuts at: 4432 4432 Size: 5926 HaeII r_GCGC'y Cuts at: 1678 5073 1678 Size: 3395 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2931 2963 4567 4599 4641 Size: 32 135 32 1359 32 1604 32 42 Cuts at: 4641 4673 1373 Size: 32 2626 Fragments arranged by size: 2626 1604 1359 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3270 3516 669 Size: 988 281 1332 246 3079 Fragments arranged by size: 3079 1332 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5926 HpaI GTT'AAC Cuts at: 3516 3516 Size: 5926 KpnI G_GTAC'C Cuts at: 3251 3251 Size: 5926 MfeI C'AATT_G Cuts at: 3525 3525 Size: 5926 MluI A'CGCG_T Cuts at: 5802 5802 Size: 5926 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2268 2649 2766 5040 5224 1305 Size: 963 381 117 2274 184 2007 Fragments arranged by size: 2274 2007 963 381 184 117 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4104 Fragments arranged by size: 4104 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3209 3884 4243 4402 518 Size: 648 2043 675 359 159 2042 Fragments arranged by size: 2043 2042 675 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2021 3342 4019 5167 5412 1606 Size: 415 1321 677 1148 245 2120 Fragments arranged by size: 2120 1321 1148 677 415 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5374 NdeI CA'TA_TG Cuts at: 387 387 Size: 5926 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5926 NotI GC'GGCC_GC Cuts at: 3373 3373 Size: 5926 NsiI A_TGCA'T Cuts at: 2886 2886 Size: 5926 NspI r_CATG'y Cuts at: 1132 1229 2888 3284 4829 1132 Size: 97 1659 396 1545 2229 Fragments arranged by size: 2229 1659 1545 396 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5926 PciI A'CATG_T Cuts at: 4825 4825 Size: 5926 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5926 PmeI GTTT'AAAC Cuts at: 3230 3230 Size: 5926 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2282 2314 3995 4027 5508 5540 2282 Size: 32 1681 32 1481 32 2668 Fragments arranged by size: 2668 1681 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5926 PsiI TTA'TAA Cuts at: 2028 3041 3496 2028 Size: 1013 455 4458 Fragments arranged by size: 4458 1013 455 PstI C_TGCA'G Cuts at: 830 2451 3278 830 Size: 1621 827 3478 Fragments arranged by size: 3478 1621 827 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5926 SacII CC_GC'GG Cuts at: 2022 2022 Size: 5926 SalI G'TCGA_C Cuts at: 1936 3268 1936 Size: 1332 4594 SbfI CC_TGCA'GG Cuts at: 3278 3278 Size: 5926 ScaI AGT'ACT Cuts at: 4174 5886 4174 Size: 1712 4214 SfcI C'TryA_G Cuts at: 826 1045 2447 3186 3274 4409 5090 5281 826 Size: 219 1402 739 88 1135 681 191 1471 Fragments arranged by size: 1471 1402 1135 739 681 219 191 88 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5926 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5926 SmaI CCC'GGG Cuts at: 1872 2485 3253 1872 Size: 613 768 4545 Fragments arranged by size: 4545 768 613 SmlI C'TyrA_G Cuts at: 820 1017 1943 2229 4030 4931 5193 820 Size: 197 926 286 1801 901 262 1553 Fragments arranged by size: 1801 1553 926 901 286 262 197 SnaBI TAC'GTA Cuts at: 493 3717 493 Size: 3224 2702 SpeI A'CTAG_T Cuts at: 152 2317 152 Size: 2165 3761 SphI G_CATG'C Cuts at: 1132 2888 3284 1132 Size: 1756 396 3774 Fragments arranged by size: 3774 1756 396 SspI AAT'ATT Cuts at: 5 52 3850 5 Size: 47 3798 2081 Fragments arranged by size: 3798 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5926 StyI C'CwwG_G Cuts at: 513 1065 3365 513 Size: 552 2300 3074 Fragments arranged by size: 3074 2300 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3953 3970 4123 4308 4753 325 Size: 1255 2373 17 153 185 445 1498 Fragments arranged by size: 2373 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4172 5884 96 Size: 274 80 33 51 1443 2195 1712 138 Fragments arranged by size: 2195 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3100 3132 3145 3177 5886 5918 3100 Size: 32 13 32 2709 32 3108 Fragments arranged by size: 3108 2709 32 32 32 13 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5926 XbaI T'CTAG_A Cuts at: 3262 3262 Size: 5926 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2338 2338 Size: 5926 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5926 XmaI C'CCGG_G Cuts at: 1870 2483 3251 1870 Size: 613 768 4545 Fragments arranged by size: 4545 768 613 XmnI GAAnn'nnTTC Cuts at: 4055 4055 Size: 5926 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3734 276 Size: 53 83 186 936 2200 2468 Fragments arranged by size: 2468 2200 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI