(Linear) (Six-base) MAPSORT of: KIEE1545.seq Check: 8892 from: 1 to: 1221 KIEE1545.orf With 182 enzymes: SgfI * September 30, 2014 10:50 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 470 1154 1221 Size: 470 684 67 Fragments arranged by size: 684 470 67 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 277 309 404 436 1221 Size: 277 32 95 32 785 Fragments arranged by size: 785 277 95 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 241 901 1221 Size: 241 660 320 Fragments arranged by size: 660 320 241 ApoI r'AATT_y Cuts at: 0 114 679 701 952 1221 Size: 114 565 22 251 269 Fragments arranged by size: 565 269 251 114 22 AseI AT'TA_AT Cuts at: 0 755 1221 Size: 755 466 AvaI C'yCGr_G Cuts at: 0 478 1221 Size: 478 743 BbsI GAAGACnn'nnnn_ Cuts at: 0 1145 1221 Size: 1145 76 BciVI GTATCCnnnnn_n' Cuts at: 0 777 1221 Size: 777 444 BclI T'GATC_A Cuts at: 0 980 1221 Size: 980 241 BfrBI ATG'CAT Cuts at: 0 879 1221 Size: 879 342 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 209 1221 Size: 209 1012 BsaI GGTCTCn'nnnn_ Cuts at: 0 295 1221 Size: 295 926 BsaWI w'CCGG_w Cuts at: 0 956 1221 Size: 956 265 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 277 307 1095 1125 1221 Size: 277 30 788 30 96 Fragments arranged by size: 788 277 96 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1204 1221 Size: 1204 17 BspHI T'CATG_A Cuts at: 0 683 944 1221 Size: 683 261 277 Fragments arranged by size: 683 277 261 BstAPI GCAn_nnn'nTGC Cuts at: 0 441 1221 Size: 441 780 BstBI TT'CG_AA Cuts at: 0 423 699 1221 Size: 423 276 522 Fragments arranged by size: 522 423 276 BstXI CCAn_nnnn'nTGG Cuts at: 0 336 1206 1221 Size: 336 870 15 Fragments arranged by size: 870 336 15 BstYI r'GATC_y Cuts at: 0 347 690 1221 Size: 347 343 531 Fragments arranged by size: 531 347 343 BtgI C'CryG_G Cuts at: 0 14 570 1221 Size: 14 556 651 Fragments arranged by size: 651 556 14 DraI TTT'AAA Cuts at: 0 119 1221 Size: 119 1102 DraIII CAC_nnn'GTG Cuts at: 0 793 1221 Size: 793 428 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 470 1154 1221 Size: 470 684 67 Fragments arranged by size: 684 470 67 EcoRI G'AATT_C Cuts at: 0 701 952 1221 Size: 701 251 269 Fragments arranged by size: 701 269 251 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 926 958 1221 Size: 926 32 263 Fragments arranged by size: 926 263 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 263 644 761 1221 Size: 263 381 117 460 Fragments arranged by size: 460 381 263 117 MslI CAynn'nnrTG Cuts at: 0 1204 1221 Size: 1204 17 MspA1I CmG'CkG Cuts at: 0 16 1221 Size: 16 1205 NsiI A_TGCA'T Cuts at: 0 881 1221 Size: 881 340 NspI r_CATG'y Cuts at: 0 883 1221 Size: 883 338 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 277 309 1221 Size: 277 32 912 Fragments arranged by size: 912 277 32 PsiI TTA'TAA Cuts at: 0 23 1036 1221 Size: 23 1013 185 Fragments arranged by size: 1013 185 23 PstI C_TGCA'G Cuts at: 0 446 1221 Size: 446 775 SacII CC_GC'GG Cuts at: 0 17 1221 Size: 17 1204 SfcI C'TryA_G Cuts at: 0 442 1181 1221 Size: 442 739 40 Fragments arranged by size: 739 442 40 SmaI CCC'GGG Cuts at: 0 480 1221 Size: 480 741 SmlI C'TyrA_G Cuts at: 0 224 1221 Size: 224 997 SpeI A'CTAG_T Cuts at: 0 312 1221 Size: 312 909 SphI G_CATG'C Cuts at: 0 883 1221 Size: 883 338 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1095 1127 1140 1172 1221 Size: 1095 32 13 32 49 Fragments arranged by size: 1095 49 32 32 13 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 333 1221 Size: 333 888 XmaI C'CCGG_G Cuts at: 0 478 1221 Size: 478 743 Enzymes that do cut: AcuI AloI AlwNI ApoI AseI AvaI BbsI BciVI BclI BfrBI BpuEI BsaI BsaWI BsaXI BseRI BspHI BstAPI BstBI BstXI BstYI BtgI DraI DraIII Eco57MI EcoRI Hin4I MmeI MslI MspA1I NsiI NspI PpiI PsiI PstI SacII SfcI SmaI SmlI SpeI SphI TstI XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BsaAI BsaBI BsaHI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DrdI EaeI EagI EarI EciI EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PflMI PfoI PmeI PmlI PpuMI PshAI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SrfI SspI StuI StyI SwaI TaqII TatI Tth111I XbaI XhoI XmnI ZraI