(Circular) (Six-base) MAPSORT of: pFN21AE1361.seq Check: 5589 from: 1 to: 5002 pFN21AE1361 5002 bp With 182 enzymes: SgfI * August 16, 2010 14:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2812 278 Size: 53 83 186 936 1276 2468 Fragments arranged by size: 2468 1276 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2345 1937 Size: 408 4594 Acc65I G'GTAC_C Cuts at: 2323 2323 Size: 5002 AclI AA'CG_TT Cuts at: 1563 3129 3502 1563 Size: 1566 373 3063 Fragments arranged by size: 3063 1566 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3065 4449 4717 1632 Size: 1433 1384 268 1917 Fragments arranged by size: 1917 1433 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4805 AflIII A'CryG_T Cuts at: 1203 3901 4878 1203 Size: 2698 977 1327 Fragments arranged by size: 2698 1327 977 AgeI A'CCGG_T Cuts at: 3833 3833 Size: 5002 AhdI GACnn_n'nnGTC Cuts at: 3731 3731 Size: 5002 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5002 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5002 AlwNI CAG_nnn'CTG Cuts at: 2745 4317 2745 Size: 1572 3430 ApaLI G'TGCA_C Cuts at: 3059 4215 3059 Size: 1156 3846 ApoI r'AATT_y Cuts at: 1493 2144 2296 2311 2537 2689 4798 1493 Size: 651 152 15 226 152 2109 1697 Fragments arranged by size: 2109 1697 651 226 152 152 15 AseI AT'TA_AT Cuts at: 160 1032 3556 160 Size: 872 2524 1606 Fragments arranged by size: 2524 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5002 AvaI C'yCGr_G Cuts at: 1870 1943 2327 1870 Size: 73 384 4545 Fragments arranged by size: 4545 384 73 BamHI G'GATC_C Cuts at: 2332 2699 2332 Size: 367 4635 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2323 3778 618 Size: 325 217 627 536 1455 1842 Fragments arranged by size: 1842 1455 627 536 325 217 BanII G_rGCy'C Cuts at: 1391 2232 2321 1391 Size: 841 89 4072 Fragments arranged by size: 4072 841 89 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4031 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3193 3227 1903 Size: 34 1256 34 3678 Fragments arranged by size: 3678 1256 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2894 4104 2894 Size: 1210 3792 BclI T'GATC_A Cuts at: 1548 1548 Size: 5002 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3613 136 Size: 107 122 71 631 605 1941 1525 Fragments arranged by size: 1941 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4997 4997 Size: 5002 BlpI GC'TnA_GC Cuts at: 2419 2419 Size: 5002 Bme1580I G_kGCm'C Cuts at: 1790 3063 4219 1790 Size: 1273 1156 2573 Fragments arranged by size: 2573 1273 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5002 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2263 2733 3691 Size: 416 53 83 399 24 846 470 958 Cuts at: 3691 442 Size: 1753 Fragments arranged by size: 1753 958 846 470 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5002 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3662 1239 Size: 2423 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3127 3992 4290 3127 Size: 865 298 3839 Fragments arranged by size: 3839 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3665 882 Size: 617 2166 2219 Fragments arranged by size: 2219 2166 617 BsaAI yAC'GTr Cuts at: 493 1204 2793 493 Size: 711 1589 2702 Fragments arranged by size: 2702 1589 711 BsaBI GATnn'nnATC Cuts at: 4996 4996 Size: 5002 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2809 3191 3826 275 Size: 53 83 186 936 1276 382 635 1451 Fragments arranged by size: 1451 1276 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3435 3833 4107 4254 4665 3435 Size: 398 274 147 411 3772 Fragments arranged by size: 3772 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4777 Fragments arranged by size: 4777 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5002 BseYI C'CCAG_C Cuts at: 4205 4701 4205 Size: 496 4506 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2091 2279 1163 Size: 573 355 188 3886 Fragments arranged by size: 3886 573 355 188 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2452 3213 4241 1540 Size: 275 186 451 761 1028 2301 Fragments arranged by size: 2301 1028 761 451 275 186 BsiHKAI G_wGCw'C Cuts at: 2232 3063 3148 4219 2232 Size: 831 85 1071 3015 Fragments arranged by size: 3015 1071 831 85 BsmI GAATG_Cn' Cuts at: 2513 2606 2513 Size: 93 4909 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2232 2321 3063 3148 4219 1391 Size: 399 442 89 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 442 399 89 85 BspEI T'CCGG_A Cuts at: 4665 4665 Size: 5002 BspHI T'CATG_A Cuts at: 2891 4621 2891 Size: 1730 3272 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2357 844 Size: 1513 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2889 1394 Size: 429 1066 3507 Fragments arranged by size: 3507 1066 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3497 3671 65 Size: 1183 2249 174 1396 Fragments arranged by size: 2249 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3646 3833 1700 Size: 110 1836 187 2869 Fragments arranged by size: 2869 1836 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5002 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5002 BssSI C'ACGA_G Cuts at: 3062 4074 3062 Size: 1012 3990 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5002 BstBI TT'CG_AA Cuts at: 3806 3806 Size: 5002 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5002 BstYI r'GATC_y Cuts at: 1972 2332 2699 3084 3101 4553 4639 4651 4997 Size: 360 367 385 17 1452 86 12 346 Cuts at: 4997 1972 Size: 1977 Fragments arranged by size: 1977 1452 385 367 360 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 3493 Fragments arranged by size: 3493 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4263 Fragments arranged by size: 4263 683 56 BtsI GCAGTG_nn' Cuts at: 2182 2518 3312 3332 2182 Size: 336 794 20 3852 Fragments arranged by size: 3852 794 336 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5002 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4967 DraI TTT'AAA Cuts at: 2306 2306 Size: 5002 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4009 809 Size: 507 2693 1802 Fragments arranged by size: 2693 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2019 2449 3338 Size: 54 1425 50 275 18 189 430 889 Cuts at: 3338 4967 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 430 275 189 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2449 1537 Size: 275 637 4090 Fragments arranged by size: 4090 637 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2218 2279 2932 1353 Size: 865 61 653 3423 Fragments arranged by size: 3423 865 653 61 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3589 3963 4109 387 Size: 1064 138 2000 374 146 1280 Fragments arranged by size: 2000 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2230 2230 Size: 5002 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3065 3662 4449 4717 1239 Size: 393 1433 597 787 268 1524 Fragments arranged by size: 1524 1433 787 597 393 268 EcoRI G'AATT_C Cuts at: 2296 2311 2689 2296 Size: 15 378 4609 Fragments arranged by size: 4609 378 15 FspI TGC'GCA Cuts at: 3508 3508 Size: 5002 HaeII r_GCGC'y Cuts at: 1678 4149 1678 Size: 2471 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3643 3675 3717 3749 1373 Size: 32 135 32 2071 32 42 32 2626 Fragments arranged by size: 2626 2071 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2346 2592 669 Size: 988 281 408 246 3079 Fragments arranged by size: 3079 988 408 281 246 HindIII A'AGCT_T Cuts at: 748 2210 748 Size: 1462 3540 HpaI GTT'AAC Cuts at: 2592 2592 Size: 5002 KpnI G_GTAC'C Cuts at: 2327 2327 Size: 5002 MfeI C'AATT_G Cuts at: 2601 2601 Size: 5002 MluI A'CGCG_T Cuts at: 4878 4878 Size: 5002 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4116 4300 1305 Size: 2811 184 2007 Fragments arranged by size: 2811 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3180 Fragments arranged by size: 3180 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2960 3319 3478 518 Size: 648 1794 359 159 2042 Fragments arranged by size: 2042 1794 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2418 3095 4243 4488 1606 Size: 812 677 1148 245 2120 Fragments arranged by size: 2120 1148 812 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4450 NdeI CA'TA_TG Cuts at: 387 387 Size: 5002 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5002 NotI GC'GGCC_GC Cuts at: 2449 2449 Size: 5002 NspI r_CATG'y Cuts at: 1132 1229 2360 3905 1132 Size: 97 1131 1545 2229 Fragments arranged by size: 2229 1545 1131 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5002 PciI A'CATG_T Cuts at: 3901 3901 Size: 5002 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5002 PmeI GTTT'AAAC Cuts at: 2306 2306 Size: 5002 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3071 3103 4584 4616 3071 Size: 32 1481 32 3457 Fragments arranged by size: 3457 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5002 PsiI TTA'TAA Cuts at: 2572 2572 Size: 5002 PstI C_TGCA'G Cuts at: 830 2056 2354 830 Size: 1226 298 3478 Fragments arranged by size: 3478 1226 298 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5002 SacI G_AGCT'C Cuts at: 2232 2232 Size: 5002 SalI G'TCGA_C Cuts at: 1936 2344 1936 Size: 408 4594 SbfI CC_TGCA'GG Cuts at: 2354 2354 Size: 5002 ScaI AGT'ACT Cuts at: 3250 4962 3250 Size: 1712 3290 SfcI C'TryA_G Cuts at: 826 1045 2052 2350 3485 4166 4357 826 Size: 219 1007 298 1135 681 191 1471 Fragments arranged by size: 1471 1135 1007 681 298 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5002 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5002 SmaI CCC'GGG Cuts at: 1872 2329 1872 Size: 457 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3106 4007 4269 820 Size: 197 926 1163 901 262 1553 Fragments arranged by size: 1553 1163 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2793 493 Size: 2300 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5002 SphI G_CATG'C Cuts at: 1132 2360 1132 Size: 1228 3774 SspI AAT'ATT Cuts at: 5 52 2926 5 Size: 47 2874 2081 Fragments arranged by size: 2874 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5002 StyI C'CwwG_G Cuts at: 513 1065 2081 2244 2441 513 Size: 552 1016 163 197 3074 Fragments arranged by size: 3074 1016 552 197 163 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2245 3029 3046 3199 3384 3829 325 Size: 1255 665 784 17 153 185 445 1498 Fragments arranged by size: 1498 1255 784 665 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3248 4960 96 Size: 274 80 33 51 1443 1271 1712 138 Fragments arranged by size: 1712 1443 1271 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4962 4994 4962 Size: 32 4970 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5002 XbaI T'CTAG_A Cuts at: 2338 2338 Size: 5002 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2020 2020 Size: 5002 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5002 XmaI C'CCGG_G Cuts at: 1870 2327 1870 Size: 457 4545 XmnI GAAnn'nnTTC Cuts at: 3131 3131 Size: 5002 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2810 276 Size: 53 83 186 936 1276 2468 Fragments arranged by size: 2468 1276 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI