################################## # NO CUT ENZYMES ( 168 enzymes) # ################################## AatII AccI AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspHI AspI AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BcnI BfrI BglI BglII BlnI BmyI BpuAI BsaAI BsaBI BsaHI BsaI BseAI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CfoI ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tth111I TthHB8I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 473 GGTACC G^GTACC AlwI 3 GGATC GGATC(4/5) AlwNI 376 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp718I 473 GGTACC G^GTACC AvaI 266 CCCGAG C^[CT]CG[GA]G AvaII 328 GGACC G^G[AT]CC BanI 473 GGTACC G^G[CT][GA]CC BciVI 511 GGATAC GTATCC(6/5) BclI 185 TGATCA T^GATCA BfaI 235 CTAG C^TAG BlpI 381 GCTGAGC GC^T[ACGT]AGC Bpu1102I 381 GCTGAGC GC^T[ACGT]AGC BsaWI 175 TCCGGT [AT]^CCGG[AT] BseRI 104 GAGGAG GAGGAG(10/8) BsgI 412 CTGCAC GTGCAG(16/14) BsmFI 322 GTCCC GGGAC(10/14) BsmI 299 GCATTC GAATGC(1/-1) BsoBI 266 CCCGAG C^[CT]CG[GA]G BspHI 458 TCATGA T^CATGA BsrFI 502 GCCGGT [GA]^CCGG[CT] BstXI 341 CCATCCTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 275 GCCTGC GC[ACGT]^[ACGT]GC CelII 381 GCTGAGC GC^T[ACGT]AGC Cfr10I 502 GCCGGT [GA]^CCGG[CT] DdeI 382 CTGAG C^T[ACGT]AG DsaI 96 CCATGG C^C[GA][CT]GG EarI 520 CTCTTC CTCTTC(1/4) EcoNI 319 CCTGTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 550 GATATC GAT^ATC EcoT14I 96 CCATGG C^C[AT][AT]GG FbaI 185 TGATCA T^GATCA HaeIII 501 GGCC GG^CC KpnI 473 GGTACC GGTAC^C Ksp632I 520 CTCTTC CTCTTC(1/4) MaeI 235 CTAG C^TAG MaeIII 288 GTCAC ^GT[ACGT]AC MseI 152 TTAA T^TAA NcoI 96 CCATGG C^CATGG NdeI 16 CATATG CA^TATG PflMI 12 CCACCATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 458 TCATGA T^CATGA SmlI 256 CTCAAG C^T[CT][GA]AG StyI 96 CCATGG C^C[AT][AT]GG Tru9I 152 TTAA T^TAA Tsp45I 288 GTCAC ^GT[GC]AC Tsp509I 310 AATT ^AATT Van91I 12 CCACCATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### BpmI 197 CTGGAG CTGGAG(16/14) 209 CTGGAG BsaJI 96 CCATGG C^C[ACGT][ACGT]GG 324 CCCAGG BsmAI 107 GAGAC GTCTC(1/5) 352 GAGAC BssKI 325 CCAGG ^CC[ACGT]GG 423 CCAGG BstAPI 372 GCAACAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 402 GCAAACAGTGC BstNI 325 CCAGG CC^[AT]GG 423 CCAGG Cfr13I 328 GGACC G^G[ACGT]CC 500 GGGCC DpnII 4 GATC ^GATC 186 GATC EcoRII 325 CCAGG ^CC[AT]GG 423 CCAGG HapII 176 CCGG C^CGG 503 CCGG HinfI 37 GACTC G^A[ACGT]TC 254 GACTC HpaII 176 CCGG C^CGG 503 CCGG MboI 4 GATC ^GATC 186 GATC MspA1I 194 CAGCTG C[AC]G^C[GT]G 525 CAGCTG MspI 176 CCGG C^CGG 503 CCGG MvaI 325 CCAGG CC^[AT]GG 423 CCAGG NdeII 4 GATC ^GATC 186 GATC PleI 37 GACTC GAGTC(4/5) 254 GACTC PvuII 194 CAGCTG CAG^CTG 525 CAGCTG Sau3AI 4 GATC ^GATC 186 GATC Sau96I 328 GGACC G^G[ACGT]CC 500 GGGCC ScrFI 325 CCAGG CC^[ACGT]GG 423 CCAGG SfaNI 126 GATGC GCATC(5/9) 378 GATGC ######################################### # 3 OR MORE CUT ENZYMES ( 20 enzymes) # ######################################### AfaI AluI BbvI BsiYI BslI BsrDI BstF5I Fnu4HI FokI HphI ItaI MboII MnlI MslI MwoI NlaIII NlaIV RsaI TseI TspRI