(Linear) (Six-base) MAPSORT of: KIEE5218.seq Check: 9830 from: 1 to: 570 KIEE5218.orf With 182 enzymes: SgfI * January 29, 2014 11:51 .. Acc65I G'GTAC_C Cuts at: 0 473 570 Size: 473 97 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 65 507 570 Size: 65 442 63 Fragments arranged by size: 442 65 63 AlwNI CAG_nnn'CTG Cuts at: 0 381 570 Size: 381 189 AvaI C'yCGr_G Cuts at: 0 266 570 Size: 266 304 BanI G'GyrC_C Cuts at: 0 473 570 Size: 473 97 BciVI GTATCCnnnnn_n' Cuts at: 0 505 570 Size: 505 65 BclI T'GATC_A Cuts at: 0 185 570 Size: 185 385 BlpI GC'TnA_GC Cuts at: 0 382 570 Size: 382 188 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 407 570 Size: 407 163 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 218 230 570 Size: 218 12 340 Fragments arranged by size: 340 218 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 241 570 Size: 241 329 BsaWI w'CCGG_w Cuts at: 0 175 570 Size: 175 395 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 203 233 570 Size: 203 30 337 Fragments arranged by size: 337 203 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 119 570 Size: 119 451 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 397 570 Size: 397 173 BsmI GAATG_Cn' Cuts at: 0 299 570 Size: 299 271 BspHI T'CATG_A Cuts at: 0 458 570 Size: 458 112 BsrDI GCAATG_nn' Cuts at: 0 245 482 561 570 Size: 245 237 79 9 Fragments arranged by size: 245 237 79 9 BsrFI r'CCGG_y Cuts at: 0 502 570 Size: 502 68 BstAPI GCAn_nnn'nTGC Cuts at: 0 378 408 570 Size: 378 30 162 Fragments arranged by size: 378 162 30 BstXI CCAn_nnnn'nTGG Cuts at: 0 348 570 Size: 348 222 BtgI C'CryG_G Cuts at: 0 96 570 Size: 96 474 BtsI GCAGTG_nn' Cuts at: 0 230 545 570 Size: 230 315 25 Fragments arranged by size: 315 230 25 EarI CTCTTCn'nnn_ Cuts at: 0 526 570 Size: 526 44 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 65 218 230 507 570 Size: 65 153 12 277 63 Fragments arranged by size: 277 153 65 63 12 EcoNI CCTnn'n_nnAGG Cuts at: 0 323 570 Size: 323 247 EcoRV GAT'ATC Cuts at: 0 552 570 Size: 552 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 503 535 570 Size: 503 32 35 Fragments arranged by size: 503 35 32 KpnI G_GTAC'C Cuts at: 0 477 570 Size: 477 93 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 65 570 Size: 65 505 MslI CAynn'nnrTG Cuts at: 0 95 121 346 424 457 570 Size: 95 26 225 78 33 113 Fragments arranged by size: 225 113 95 78 33 26 MspA1I CmG'CkG Cuts at: 0 196 527 570 Size: 196 331 43 Fragments arranged by size: 331 196 43 NcoI C'CATG_G Cuts at: 0 96 570 Size: 96 474 NdeI CA'TA_TG Cuts at: 0 17 570 Size: 17 553 PflMI CCAn_nnn'nTGG Cuts at: 0 18 570 Size: 18 552 PvuII CAG'CTG Cuts at: 0 196 527 570 Size: 196 331 43 Fragments arranged by size: 331 196 43 SmlI C'TyrA_G Cuts at: 0 256 570 Size: 256 314 StyI C'CwwG_G Cuts at: 0 96 570 Size: 96 474 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 86 570 Size: 86 484 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 203 235 570 Size: 203 32 335 Fragments arranged by size: 335 203 32 Enzymes that do cut: Acc65I AcuI AlwNI AvaI BanI BciVI BclI BlpI BplI BpmI BpuEI BsaWI BsaXI BseRI BsgI BsmI BspHI BsrDI BsrFI BstAPI BstXI BtgI BtsI EarI Eco57MI EcoNI EcoRV Hin4I KpnI MmeI MslI MspA1I NcoI NdeI PflMI PvuII SmlI StyI TaqII TstI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanII BbeI BbsI BbvCI BcgI BfrBI BglI BglII Bme1580I BmgBI BmrI BmtI Bpu10I BsaI BsaAI BsaBI BsaHI BseYI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspMI BsrBI BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoO109I EcoRI FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI MfeI MluI MscI NaeI NarI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI