################################## # NO CUT ENZYMES ( 194 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsiEI BsiWI BsiYI BslI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT14I EcoT22I FbaI FseI FspI HaeII HapII HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI Ksp632I KspI MamI MboII MfeI MflI MluI MluNI MroI MscI MspA1I MspI MunI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AfaI 21 GTAC GT^AC AflIII 563 ACATGT A^C[GA][CT]GT AspHI 123 GAGCAC G[AT]GC[AT]^C BbvI 487 GCTGC GCAGC(8/12) BfaI 466 CTAG C^TAG BsgI 628 GTGCAG GTGCAG(16/14) BsiHKAI 123 GAGCAC G[AT]GC[AT]^C BsmFI 194 GGGAC GGGAC(10/14) BspLU11I 563 ACATGT A^CATGT BsrDI 276 CATTGC GCAATG(2/0) BssKI 159 CCAGG ^CC[ACGT]GG BstF5I 156 CATCC GGATG(2/0) BstNI 159 CCAGG CC^[AT]GG BstXI 159 CCAGGTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr13I 462 GGCCC G^G[ACGT]CC DraI 34 TTTAAA TTT^AAA EcoRII 159 CCAGG ^CC[AT]GG EcoRV 215 GATATC GAT^ATC FauI 8 CCCGC CCCGC(4/6) Fnu4HI 487 GCTGC GC^[ACGT]GC FokI 156 CATCC GGATG(9/13) HaeIII 462 GGCC GG^CC HgaI 551 GACGC GACGC(5/10) ItaI 487 GCTGC GC^[ACGT]GC MaeI 466 CTAG C^TAG MaeII 384 ACGT A^CGT MaeIII 344 GTAAC ^GT[ACGT]AC MnlI 30 CCTC CCTC(7/6) MvaI 159 CCAGG CC^[AT]GG NdeI 293 CATATG CA^TATG NspI 563 ACATGT [GA]CATG^[CT] PleI 469 GACTC GAGTC(4/5) RsaI 21 GTAC GT^AC Sau96I 462 GGCCC G^G[ACGT]CC ScrFI 159 CCAGG CC^[ACGT]GG SfaNI 155 GCATC GCATC(5/9) TfiI 55 GATTC G^A[AT]TC TseI 487 GCTGC G^C[AT]GC Tsp509I 501 AATT ^AATT ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AccII 10 CGCG CG^CG 553 CGCG AciI 9 CCGC CCGC(-3/-1) 519 CCGC BanI 4 GGCACC G^G[CT][GA]CC 256 GGTGCC BmyI 3 GGGCAC G[AGT]GC[ACT]^C 123 GAGCAC BsmAI 84 GTCTC GTCTC(1/5) 567 GTCTC Bsp1286I 3 GGGCAC G[AGT]GC[ACT]^C 123 GAGCAC BstUI 10 CGCG CG^CG 553 CGCG DpnII 331 GATC ^GATC 379 GATC HinfI 55 GATTC G^A[ACGT]TC 469 GACTC MboI 331 GATC ^GATC 379 GATC MslI 185 CACAGATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 357 CATGCTTGTG MvnI 10 CGCG CG^CG 553 CGCG MwoI 250 GCTGTAGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 621 GCCAAAGGTGC NdeII 331 GATC ^GATC 379 GATC Sau3AI 331 GATC ^GATC 379 GATC SfcI 251 CTGTAG C^T[GA][CT]AG 404 CTACAG TspRI 609 CACTG CA[GC]TG[ACGT][ACGT]^ 631 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 8 enzymes) # ######################################### AluI DdeI MseI NlaIII NlaIV TaqI Tru9I TthHB8I