################################## # NO CUT ENZYMES ( 179 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaJI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HapII HgaI Hin1I HpaI HpaII KasI KpnI Ksp632I KspI MamI MluI MluNI MroI MscI MspI MvaI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AcsI 274 GAATTT [GA]^AATT[CT] AflIII 487 ACACGT A^C[GA][CT]GT ApoI 274 GAATTT [GA]^AATT[CT] BclI 690 TGATCA T^GATCA BmyI 222 GGGCAC G[AGT]GC[ACT]^C BsaAI 610 TACGTA [CT]AC^GT[GA] BsaI 1329 GAGACC GGTCTC(1/5) BseRI 447 CTCCTC GAGGAG(10/8) BsmAI 1329 GAGAC GTCTC(1/5) BsmFI 194 GTCCC GGGAC(10/14) Bsp1286I 222 GGGCAC G[AGT]GC[ACT]^C BspHI 1028 TCATGA T^CATGA BstXI 844 CCACTTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 255 GGATCT [GA]^GATC[CT] Cac8I 642 GCTTGC GC[ACGT]^[ACGT]GC CfoI 33 GCGC GCG^C EcoNI 82 CCTCAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 690 TGATCA T^GATCA HaeIII 991 GGCC GG^CC HhaI 33 GCGC GCG^C HinP1I 33 GCGC G^CGC MfeI 1226 CAATTG C^AATTG MflI 255 GGATCT [GA]^GATC[CT] MslI 621 CACAACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 1226 CAATTG C^AATTG NdeI 26 CATATG CA^TATG NspI 791 ACATGC [GA]CATG^[CT] RcaI 1028 TCATGA T^CATGA SfcI 107 CTGTAG C^T[GA][CT]AG SnaBI 610 TACGTA TAC^GTA TspRI 632 CAGTG CA[GC]TG[ACGT][ACGT]^ XcmI 841 CCACCACTTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 255 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AfaI 836 GTAC GT^AC 1287 GTAC AlwNI 305 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG 1354 CAGCTGCTG BanI 223 GGCACC G^G[CT][GA]CC 1065 GGCACC BsgI 412 GTGCAG GTGCAG(16/14) 881 GTGCAG BsiYI 82 CCTCAAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 993 CCTTGCACGGG BslI 82 CCTCAAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 993 CCTTGCACGGG BsrDI 806 CATTGC GCAATG(2/0) 649 GCAATG HincII 655 GTTGAC GT[CT]^[GA]AC 719 GTCAAC HindII 655 GTTGAC GT[CT]^[GA]AC 719 GTCAAC HphI 130 GGTGA GGTGA(8/7) 244 GGTGA MaeII 489 ACGT A^CGT 611 ACGT MaeIII 383 GTGAC ^GT[ACGT]AC 526 GTCAC MspA1I 1154 CAGCTG C[AC]G^C[GT]G 1354 CAGCTG NlaIV 223 GGCACC GG[ACGT]^[ACGT]CC 1065 GGCACC PleI 445 GACTC GAGTC(4/5) 818 GACTC PvuII 1154 CAGCTG CAG^CTG 1354 CAGCTG RsaI 836 GTAC GT^AC 1287 GTAC TfiI 417 GAATC G^A[AT]TC 1264 GATTC Tsp45I 383 GTGAC ^GT[GC]AC 526 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AluI AlwI BbvI BfaI BsrI BstF5I DdeI DpnII Fnu4HI FokI HindIII HinfI ItaI MaeI MboI MboII MnlI MseI MwoI NdeII NlaIII Sau3AI SfaNI SmlI Tru9I TseI Tsp509I