(Circular) (Six-base) MAPSORT of: pFN21AB5844.seq Check: 2571 from: 1 to: 6157 pFN21AB5844 6157 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2763 2763 Size: 6157 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3967 278 Size: 53 83 186 936 2431 2468 Fragments arranged by size: 2468 2431 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3500 1937 Size: 1563 4594 Acc65I G'GTAC_C Cuts at: 3478 3478 Size: 6157 AclI AA'CG_TT Cuts at: 1563 4284 4657 1563 Size: 2721 373 3063 Fragments arranged by size: 3063 2721 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2396 2640 3431 4220 5604 5872 1632 Size: 764 244 791 789 1384 268 1917 Fragments arranged by size: 1917 1384 791 789 764 268 244 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5960 AflIII A'CryG_T Cuts at: 1203 2445 3454 5056 6033 1203 Size: 1242 1009 1602 977 1327 Fragments arranged by size: 1602 1327 1242 1009 977 AgeI A'CCGG_T Cuts at: 4988 4988 Size: 6157 AhdI GACnn_n'nnGTC Cuts at: 2370 2485 4886 2370 Size: 115 2401 3641 Fragments arranged by size: 3641 2401 115 AleI CACnn'nnGTG Cuts at: 1166 3239 1166 Size: 2073 4084 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3188 1184 Size: 2004 4153 AlwNI CAG_nnn'CTG Cuts at: 3900 5472 3900 Size: 1572 4585 ApaI G_GGCC'C Cuts at: 3025 3025 Size: 6157 ApaLI G'TGCA_C Cuts at: 4214 5370 4214 Size: 1156 5001 ApoI r'AATT_y Cuts at: 1493 2523 3119 3300 3368 3466 3692 3844 5953 Size: 1030 596 181 68 98 226 152 2109 Cuts at: 5953 1493 Size: 1697 Fragments arranged by size: 2109 1697 1030 596 226 181 152 98 68 AseI AT'TA_AT Cuts at: 160 1032 4711 160 Size: 872 3679 1606 Fragments arranged by size: 3679 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6157 AvaI C'yCGr_G Cuts at: 1870 1943 2098 2258 2849 2951 3025 3482 1870 Size: 73 155 160 591 102 74 457 4545 Fragments arranged by size: 4545 591 457 160 155 102 74 73 BamHI G'GATC_C Cuts at: 3487 3854 3487 Size: 367 5790 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2027 3248 3478 4933 618 Size: 325 217 627 240 1221 230 1455 1842 Fragments arranged by size: 1842 1455 1221 627 325 240 230 217 BanII G_rGCy'C Cuts at: 1391 2243 3025 3142 3476 1391 Size: 852 782 117 334 4072 Fragments arranged by size: 4072 852 782 334 117 BbeI G_GCGC'C Cuts at: 2031 3252 2031 Size: 1221 4936 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5186 BbvCI CC'TCA_GC Cuts at: 2991 2991 Size: 6157 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2863 2897 4348 4382 1903 Size: 34 926 34 1451 34 3678 Fragments arranged by size: 3678 1451 926 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4049 5259 4049 Size: 1210 4947 BclI T'GATC_A Cuts at: 1548 1548 Size: 6157 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2075 3162 4768 Size: 107 122 71 631 605 403 1087 1606 Cuts at: 4768 136 Size: 1525 Fragments arranged by size: 1606 1525 1087 631 605 403 122 107 71 BglII A'GATC_T Cuts at: 6152 6152 Size: 6157 BlpI GC'TnA_GC Cuts at: 3574 3574 Size: 6157 Bme1580I G_kGCm'C Cuts at: 1790 3025 4218 5374 1790 Size: 1235 1193 1156 2573 Fragments arranged by size: 2573 1235 1193 1156 BmgBI CAC'GTC Cuts at: 1318 3433 1318 Size: 2115 4042 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2397 3888 4846 Size: 416 53 83 399 24 980 1491 958 Cuts at: 4846 442 Size: 1753 Fragments arranged by size: 1753 1491 980 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6157 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2647 2647 Size: 6157 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2144 2582 2684 3345 4817 1239 Size: 905 438 102 661 1472 2579 Fragments arranged by size: 2579 1472 905 661 438 102 Bpu10I CC'TnA_GC Cuts at: 2837 2991 2837 Size: 154 6003 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3122 4282 5147 5445 3122 Size: 1160 865 298 3834 Fragments arranged by size: 3834 1160 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2723 2927 3421 4820 882 Size: 617 1224 204 494 1399 2219 Fragments arranged by size: 2219 1399 1224 617 494 204 BsaAI yAC'GTr Cuts at: 493 1204 2446 3948 493 Size: 711 1242 1502 2702 Fragments arranged by size: 2702 1502 1242 711 BsaBI GATnn'nnATC Cuts at: 6151 6151 Size: 6157 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2028 3249 3964 4346 Size: 53 83 186 936 495 1221 715 382 Cuts at: 4346 4981 275 Size: 635 1451 Fragments arranged by size: 1451 1221 936 715 635 495 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3134 4590 4988 5262 5409 5820 3134 Size: 1456 398 274 147 411 3471 Fragments arranged by size: 3471 1456 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5932 Fragments arranged by size: 5932 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2956 3278 1186 Size: 1770 322 4065 Fragments arranged by size: 4065 1770 322 BseYI C'CCAG_C Cuts at: 2637 5360 5856 2637 Size: 2723 496 2938 Fragments arranged by size: 2938 2723 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2090 2588 2599 1163 Size: 573 354 498 11 4721 Fragments arranged by size: 4721 573 498 354 11 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3019 3607 4368 5396 1540 Size: 275 186 1018 588 761 1028 2301 Fragments arranged by size: 2301 1028 1018 761 588 275 186 BsiHKAI G_wGCw'C Cuts at: 2265 4218 4303 5374 2265 Size: 1953 85 1071 3048 Fragments arranged by size: 3048 1953 1071 85 BsmI GAATG_Cn' Cuts at: 3668 3761 3668 Size: 93 6064 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2243 2265 3025 3142 3476 4218 4303 Size: 399 453 22 760 117 334 742 85 Cuts at: 4303 5374 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 760 742 453 399 334 117 85 22 BspEI T'CCGG_A Cuts at: 3134 5820 3134 Size: 2686 3471 BspHI T'CATG_A Cuts at: 4046 5776 4046 Size: 1730 4427 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2763 3512 844 Size: 1919 749 3489 Fragments arranged by size: 3489 1919 749 BsrBI CCG'CTC Cuts at: 1394 1823 2158 4044 1394 Size: 429 335 1886 3507 Fragments arranged by size: 3507 1886 429 335 BsrDI GCAATG_nn' Cuts at: 65 1248 4652 4826 65 Size: 1183 3404 174 1396 Fragments arranged by size: 3404 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2841 4801 4988 1700 Size: 110 1031 1960 187 2869 Fragments arranged by size: 2869 1960 1031 187 110 BsrGI T'GTAC_A Cuts at: 96 2223 96 Size: 2127 4030 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6157 BssSI C'ACGA_G Cuts at: 4217 5229 4217 Size: 1012 5145 BstAPI GCAn_nnn'nTGC Cuts at: 794 2536 2566 794 Size: 1742 30 4385 Fragments arranged by size: 4385 1742 30 BstBI TT'CG_AA Cuts at: 4961 4961 Size: 6157 BstEII G'GTnAC_C Cuts at: 1184 2164 1184 Size: 980 5177 BstXI CCAn_nnnn'nTGG Cuts at: 2409 2606 3241 2409 Size: 197 635 5325 Fragments arranged by size: 5325 635 197 BstYI r'GATC_y Cuts at: 1972 2925 3487 3854 4239 4256 5708 5794 5806 Size: 953 562 367 385 17 1452 86 12 Cuts at: 5806 6152 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 953 562 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2939 3158 2939 Size: 219 5938 BtgI C'CryG_G Cuts at: 513 1065 2004 3060 3234 513 Size: 552 939 1056 174 3436 Fragments arranged by size: 3436 1056 939 552 174 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5418 Fragments arranged by size: 5418 683 56 BtsI GCAGTG_nn' Cuts at: 2479 2648 3673 4467 4487 2479 Size: 169 1025 794 20 4149 Fragments arranged by size: 4149 1025 794 169 20 ClaI AT'CG_AT Cuts at: 1554 2745 1554 Size: 1191 4966 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2785 2820 529 Size: 35 2221 35 3866 Fragments arranged by size: 3866 2221 35 35 DraI TTT'AAA Cuts at: 3461 3461 Size: 6157 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2312 5164 809 Size: 507 996 2852 1802 Fragments arranged by size: 2852 1802 996 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3063 3216 3604 Size: 54 1425 50 275 18 1233 153 388 Cuts at: 3604 4493 6122 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1233 889 388 275 153 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3604 1537 Size: 275 1792 4090 Fragments arranged by size: 4090 1792 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2372 2616 3309 4087 1353 Size: 1019 244 693 778 3423 Fragments arranged by size: 3423 1019 778 693 244 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 4744 5118 5264 387 Size: 1064 138 435 2720 374 146 1280 Fragments arranged by size: 2720 1280 1064 435 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2144 2396 2582 2640 2684 3345 3431 Size: 393 512 252 186 58 44 661 86 Cuts at: 3431 4220 4817 5604 5872 1239 Size: 789 597 787 268 1524 Fragments arranged by size: 1524 789 787 661 597 512 393 268 252 186 86 58 44 EcoO109I rG'GnC_Cy Cuts at: 2548 3022 2548 Size: 474 5683 EcoRI G'AATT_C Cuts at: 2523 3368 3466 3844 2523 Size: 845 98 378 4836 Fragments arranged by size: 4836 845 378 98 FspI TGC'GCA Cuts at: 4663 4663 Size: 6157 HaeII r_GCGC'y Cuts at: 1678 2031 3252 5304 1678 Size: 353 1221 2052 2531 Fragments arranged by size: 2531 2052 1221 353 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2356 2388 2471 2503 2940 Size: 32 135 32 784 32 83 32 437 Cuts at: 2940 2972 3097 3129 4798 4830 4872 4904 1373 Size: 32 125 32 1669 32 42 32 2626 Fragments arranged by size: 2626 1669 784 437 135 125 83 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2364 2770 3309 3501 3747 669 Size: 988 281 426 406 539 192 246 3079 Fragments arranged by size: 3079 988 539 426 406 281 246 192 HindIII A'AGCT_T Cuts at: 748 748 Size: 6157 HpaI GTT'AAC Cuts at: 3747 3747 Size: 6157 KasI G'GCGC_C Cuts at: 2027 3248 2027 Size: 1221 4936 KpnI G_GTAC'C Cuts at: 3482 3482 Size: 6157 MfeI C'AATT_G Cuts at: 3756 3756 Size: 6157 MluI A'CGCG_T Cuts at: 6033 6033 Size: 6157 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2820 3315 5271 5455 1305 Size: 1515 495 1956 184 2007 Fragments arranged by size: 2007 1956 1515 495 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3218 10 Size: 54 1425 343 1386 2949 Fragments arranged by size: 2949 1425 1386 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2407 3239 4115 4474 4633 518 Size: 648 1241 832 876 359 159 2042 Fragments arranged by size: 2042 1241 876 832 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2995 3393 3573 4250 5398 5643 1606 Size: 1389 398 180 677 1148 245 2120 Fragments arranged by size: 2120 1389 1148 677 398 245 180 NaeI GCC'GGC Cuts at: 2843 2843 Size: 6157 NarI GG'CG_CC Cuts at: 2028 3249 2028 Size: 1221 4936 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4666 Fragments arranged by size: 4666 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6157 NgoMIV G'CCGG_C Cuts at: 2841 2841 Size: 6157 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6157 NotI GC'GGCC_GC Cuts at: 3604 3604 Size: 6157 NspI r_CATG'y Cuts at: 1132 1229 2224 2536 2719 3515 5060 1132 Size: 97 995 312 183 796 1545 2229 Fragments arranged by size: 2229 1545 995 796 312 183 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6157 PciI A'CATG_T Cuts at: 5056 5056 Size: 6157 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6157 PfoI T'CCnGG_A Cuts at: 2661 2928 2661 Size: 267 5890 PmeI GTTT'AAAC Cuts at: 3461 3461 Size: 6157 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4226 4258 5739 5771 4226 Size: 32 1481 32 4612 Fragments arranged by size: 4612 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2548 2548 Size: 6157 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6157 PsiI TTA'TAA Cuts at: 3727 3727 Size: 6157 PspOMI G'GGCC_C Cuts at: 3021 3021 Size: 6157 PstI C_TGCA'G Cuts at: 830 2474 3509 830 Size: 1644 1035 3478 Fragments arranged by size: 3478 1644 1035 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6157 PvuII CAG'CTG Cuts at: 3393 3393 Size: 6157 SalI G'TCGA_C Cuts at: 1936 3499 1936 Size: 1563 4594 SapI GCTCTTCn'nnn_ Cuts at: 2616 2616 Size: 6157 SbfI CC_TGCA'GG Cuts at: 3509 3509 Size: 6157 ScaI AGT'ACT Cuts at: 2183 4405 6117 2183 Size: 2222 1712 2223 Fragments arranged by size: 2223 2222 1712 SfcI C'TryA_G Cuts at: 826 1045 2470 3505 4640 5321 5512 826 Size: 219 1425 1035 1135 681 191 1471 Fragments arranged by size: 1471 1425 1135 1035 681 219 191 SfoI GGC'GCC Cuts at: 2029 3250 2029 Size: 1221 4936 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6157 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6157 SmaI CCC'GGG Cuts at: 1872 2100 3484 1872 Size: 228 1384 4545 Fragments arranged by size: 4545 1384 228 SmlI C'TyrA_G Cuts at: 820 1017 1943 2951 3101 4261 5162 5424 820 Size: 197 926 1008 150 1160 901 262 1553 Fragments arranged by size: 1553 1160 1008 926 901 262 197 150 SnaBI TAC'GTA Cuts at: 493 3948 493 Size: 3455 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6157 SphI G_CATG'C Cuts at: 1132 3515 1132 Size: 2383 3774 SspI AAT'ATT Cuts at: 5 52 4081 5 Size: 47 4029 2081 Fragments arranged by size: 4029 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6157 StyI C'CwwG_G Cuts at: 513 1065 2004 2205 3596 513 Size: 552 939 201 1391 3074 Fragments arranged by size: 3074 1391 939 552 201 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2180 3326 4184 4201 4354 4539 4984 Size: 1255 600 1146 858 17 153 185 445 Cuts at: 4984 325 Size: 1498 Fragments arranged by size: 1498 1255 1146 858 600 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 2223 3387 Size: 274 80 33 51 1443 204 42 1164 Cuts at: 3387 4403 6115 96 Size: 1016 1712 138 Fragments arranged by size: 1712 1443 1164 1016 274 204 138 80 51 42 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6117 6149 6117 Size: 32 6125 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6157 XbaI T'CTAG_A Cuts at: 3493 3493 Size: 6157 XhoI C'TCGA_G Cuts at: 1943 2951 1943 Size: 1008 5149 XmaI C'CCGG_G Cuts at: 1870 2098 3482 1870 Size: 228 1384 4545 Fragments arranged by size: 4545 1384 228 XmnI GAAnn'nnTTC Cuts at: 2465 4286 2465 Size: 1821 4336 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3965 276 Size: 53 83 186 936 2431 2468 Fragments arranged by size: 2468 2431 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI