################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BamHI BbrPI BbsI BcgI BciVI BcnI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstUI BstYI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco52I EcoNI EcoRI EcoRV EcoT22I FseI HincII HindII HindIII HinfI HpaI KpnI Ksp632I KspI MaeI MfeI MflI MluI MroI MseI MunI MvnI NciI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AatII 553 GACGTC GACGT^C AcsI 151 GAATTT [GA]^AATT[CT] AocI 600 CCTCAGG CC^T[ACGT]AGG Aor51HI 302 AGCGCT AGC^GCT ApoI 151 GAATTT [GA]^AATT[CT] AviII 443 TGCGCA TGC^GCA BalI 674 TGGCCA TGG^CCA BbeI 127 GGCGCC GGCGC^C BbvCI 542 CCTCAGC CCTCAGC(-5/-2) BclI 425 TGATCA T^GATCA BpmI 616 CTCCAG CTGGAG(16/14) BsaBI 426 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 649 GAGACC GGTCTC(1/5) BseRI 598 CTCCTC GAGGAG(10/8) BsmAI 649 GAGAC GTCTC(1/5) BsmFI 742 GTCCC GGGAC(10/14) BspMI 527 ACCTGC ACCTGC(4/8) BsrFI 259 GCCGGC [GA]^CCGG[CT] BssSI 697 CTCGTG CACGAG(-5/-1) BstEII 342 GGTGACC G^GT[ACGT]ACC BstPI 342 GGTGACC G^GT[ACGT]ACC BstXI 677 CCATCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 600 CCTCAGG CC^T[ACGT]AGG Cfr10I 259 GCCGGC [GA]^CCGG[CT] DpnII 426 GATC ^GATC DraII 581 GGGGCCT [GA]G^G[ACGT]CC[CT] Eco47III 302 AGCGCT AGC^GCT Eco81I 600 CCTCAGG CC^T[ACGT]AGG EcoO109I 581 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoO65I 342 GGTGACC G^GT[ACGT]ACC FbaI 425 TGATCA T^GATCA FspI 443 TGCGCA TGC^GCA HapII 260 CCGG C^CGG HgaI 207 GCGTC GACGC(5/10) HpaII 260 CCGG C^CGG KasI 127 GGCGCC G^GCGCC MamI 426 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 426 GATC ^GATC MluNI 674 TGGCCA TGG^CCA MscI 674 TGGCCA TGG^CCA MspA1I 166 CAGCTG C[AC]G^C[GT]G MspI 260 CCGG C^CGG NaeI 259 GCCGGC GCC^GGC NarI 127 GGCGCC GG^CGCC NcoI 644 CCATGG C^CATGG NdeI 728 CATATG CA^TATG NdeII 426 GATC ^GATC NgoMIV 259 GCCGGC G^CCGGC PshAI 651 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 166 CAGCTG CAG^CTG SacI 613 GAGCTC GAGCT^C Sau3AI 426 GATC ^GATC SfoI 127 GGCGCC GGC^GCC Tsp509I 152 AATT ^AATT ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AvaI 132 CTCGGG C^[CT]CG[GA]G 362 CTCGGG BbvI 314 GCAGC GCAGC(8/12) 441 GCTGC BglI 73 GCCACCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 683 GCCTGGTGGGC BsoBI 132 CTCGGG C^[CT]CG[GA]G 362 CTCGGG DsaI 337 CCACGG C^C[GA][CT]GG 644 CCATGG EcoT14I 77 CCAAGG C^C[AT][AT]GG 644 CCATGG HaeII 127 GGCGCC [GA]GCGC^[CT] 302 AGCGCT MaeII 329 ACGT A^CGT 554 ACGT MaeIII 343 GTGAC ^GT[ACGT]AC 460 GTCAC MboII 186 GAAGA GAAGA(8/7) 413 TCTTC StyI 77 CCAAGG C^C[AT][AT]GG 644 CCATGG TseI 314 GCAGC G^C[AT]GC 441 GCTGC Tsp45I 343 GTGAC ^GT[GC]AC 460 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AciI AcyI AluI AspHI AvaII BanI BanII BmyI BsaHI BsaJI BsiHKAI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI EaeI EcoRII FauI Fnu4HI FokI HaeIII HhaI Hin1I HinP1I HphI ItaI MnlI MslI MvaI MwoI NlaIII NlaIV Sau96I ScrFI SfaNI TspRI XcmI