(Circular) (Six-base) MAPSORT of: pFN21AE6210.seq Check: 5299 from: 1 to: 5455 pFN21AE6210 5455 bp With 182 enzymes: SgfI * March 18, 2015 14:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2562 3265 278 Size: 53 83 186 936 1026 703 2468 Fragments arranged by size: 2468 1026 936 703 186 83 53 AccI GT'mk_AC Cuts at: 1937 2798 1937 Size: 861 4594 Acc65I G'GTAC_C Cuts at: 2776 2776 Size: 5455 AclI AA'CG_TT Cuts at: 1563 3582 3955 1563 Size: 2019 373 3063 Fragments arranged by size: 3063 2019 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2210 3518 4902 5170 1632 Size: 578 1308 1384 268 1917 Fragments arranged by size: 1917 1384 1308 578 268 AfeI AGC'GCT Cuts at: 2309 2309 Size: 5455 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5258 AflIII A'CryG_T Cuts at: 1203 4354 5331 1203 Size: 3151 977 1327 Fragments arranged by size: 3151 1327 977 AgeI A'CCGG_T Cuts at: 4286 4286 Size: 5455 AhdI GACnn_n'nnGTC Cuts at: 4184 4184 Size: 5455 AleI CACnn'nnGTG Cuts at: 1166 2735 1166 Size: 1569 3886 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5455 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2159 2191 2159 Size: 32 5423 AlwNI CAG_nnn'CTG Cuts at: 3198 4770 3198 Size: 1572 3883 ApaLI G'TGCA_C Cuts at: 3512 4668 3512 Size: 1156 4299 ApoI r'AATT_y Cuts at: 1493 2156 2764 2990 3142 5251 1493 Size: 663 608 226 152 2109 1697 Fragments arranged by size: 2109 1697 663 608 226 152 AseI AT'TA_AT Cuts at: 160 1032 4009 160 Size: 872 2977 1606 Fragments arranged by size: 2977 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5455 AvaI C'yCGr_G Cuts at: 1870 1943 2137 2367 2780 1870 Size: 73 194 230 413 4545 Fragments arranged by size: 4545 413 230 194 73 BamHI G'GATC_C Cuts at: 2785 3152 2785 Size: 367 5088 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2132 2626 2708 2776 4231 Size: 325 217 627 345 494 82 68 1455 Cuts at: 4231 618 Size: 1842 Fragments arranged by size: 1842 1455 627 494 345 325 217 82 68 BanII G_rGCy'C Cuts at: 1391 2063 2102 2622 2774 1391 Size: 672 39 520 152 4072 Fragments arranged by size: 4072 672 520 152 39 BbeI G_GCGC'C Cuts at: 2136 2136 Size: 5455 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4484 BbvCI CC'TCA_GC Cuts at: 2548 2548 Size: 5455 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3646 3680 1903 Size: 34 1709 34 3678 Fragments arranged by size: 3678 1709 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3347 4557 3347 Size: 1210 4245 BclI T'GATC_A Cuts at: 1548 2430 1548 Size: 882 4573 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2084 2694 4066 Size: 107 122 71 631 605 412 610 1372 Cuts at: 4066 136 Size: 1525 Fragments arranged by size: 1525 1372 631 610 605 412 122 107 71 BglII A'GATC_T Cuts at: 5450 5450 Size: 5455 BlpI GC'TnA_GC Cuts at: 2872 2872 Size: 5455 Bme1580I G_kGCm'C Cuts at: 1790 2547 2671 2711 2732 3516 4672 1790 Size: 757 124 40 21 784 1156 2573 Fragments arranged by size: 2573 1156 784 757 124 40 21 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5455 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2593 3186 4144 Size: 416 53 83 399 24 1176 593 958 Cuts at: 4144 442 Size: 1753 Fragments arranged by size: 1753 1176 958 593 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5455 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2606 4115 1239 Size: 1367 1509 2579 Fragments arranged by size: 2579 1509 1367 Bpu10I CC'TnA_GC Cuts at: 2057 2548 2057 Size: 491 4964 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3580 4445 4743 3580 Size: 865 298 4292 Fragments arranged by size: 4292 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2648 4118 882 Size: 617 1149 1470 2219 Fragments arranged by size: 2219 1470 1149 617 BsaAI yAC'GTr Cuts at: 493 1204 3246 493 Size: 711 2042 2702 Fragments arranged by size: 2702 2042 711 BsaBI GATnn'nnATC Cuts at: 2435 5449 2435 Size: 3014 2441 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2133 2212 2559 3262 Size: 53 83 186 936 600 79 347 703 Cuts at: 3262 3644 4279 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 703 635 600 382 347 186 83 79 53 BsaWI w'CCGG_w Cuts at: 3888 4286 4560 4707 5118 3888 Size: 398 274 147 411 4225 Fragments arranged by size: 4225 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2084 2114 1178 Size: 30 165 30 681 30 4519 Fragments arranged by size: 4519 681 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2594 1186 Size: 1408 4047 BseYI C'CCAG_C Cuts at: 2377 2499 4658 5154 2377 Size: 122 2159 496 2678 Fragments arranged by size: 2678 2159 496 122 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4882 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2905 3666 4694 1540 Size: 275 186 904 761 1028 2301 Fragments arranged by size: 2301 1028 904 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2358 2583 2622 3516 3601 4672 2358 Size: 225 39 894 85 1071 3141 Fragments arranged by size: 3141 1071 894 225 85 39 BsmI GAATG_Cn' Cuts at: 2966 3059 2966 Size: 93 5362 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2063 2102 2358 2547 2583 2622 2671 Size: 399 273 39 256 189 36 39 49 Cuts at: 2671 2711 2732 2774 3516 3601 4672 1391 Size: 40 21 42 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 273 256 189 85 49 42 40 39 39 36 21 BspEI T'CCGG_A Cuts at: 5118 5118 Size: 5455 BspHI T'CATG_A Cuts at: 3344 5074 3344 Size: 1730 3725 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2541 2810 844 Size: 1697 269 3489 Fragments arranged by size: 3489 1697 269 BsrBI CCG'CTC Cuts at: 1394 1823 3342 1394 Size: 429 1519 3507 Fragments arranged by size: 3507 1519 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3950 4124 65 Size: 1183 2702 174 1396 Fragments arranged by size: 2702 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2264 4099 4286 1700 Size: 110 454 1835 187 2869 Fragments arranged by size: 2869 1835 454 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5455 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5455 BssSI C'ACGA_G Cuts at: 2702 3515 4527 2702 Size: 813 1012 3630 Fragments arranged by size: 3630 1012 813 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5455 BstBI TT'CG_AA Cuts at: 4259 4259 Size: 5455 BstEII G'GTnAC_C Cuts at: 1184 2347 1184 Size: 1163 4292 BstXI CCAn_nnnn'nTGG Cuts at: 2689 2689 Size: 5455 BstYI r'GATC_y Cuts at: 1972 2785 3152 3537 3554 5006 5092 5104 5450 Size: 813 367 385 17 1452 86 12 346 Cuts at: 5450 1972 Size: 1977 Fragments arranged by size: 1977 1452 813 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2606 2606 Size: 5455 BtgI C'CryG_G Cuts at: 513 1065 2004 2342 2649 513 Size: 552 939 338 307 3319 Fragments arranged by size: 3319 939 552 338 307 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2254 487 Size: 683 56 1028 3688 Fragments arranged by size: 3688 1028 683 56 BtsI GCAGTG_nn' Cuts at: 2971 3765 3785 2971 Size: 794 20 4641 Fragments arranged by size: 4641 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5455 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5420 DraI TTT'AAA Cuts at: 2759 2759 Size: 5455 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4462 809 Size: 507 3146 1802 Fragments arranged by size: 3146 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2262 2455 2679 Size: 54 1425 50 275 18 432 193 224 Cuts at: 2679 2902 3791 5420 8 Size: 223 889 1629 43 Fragments arranged by size: 1629 1425 889 432 275 224 223 193 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2902 1537 Size: 275 1090 4090 Fragments arranged by size: 4090 1090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3385 1353 Size: 2032 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4042 4416 4562 387 Size: 1064 138 2453 374 146 1280 Fragments arranged by size: 2453 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2620 2620 Size: 5455 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2210 2606 3518 4115 4902 5170 1239 Size: 393 578 396 912 597 787 268 1524 Fragments arranged by size: 1524 912 787 597 578 396 393 268 EcoO109I rG'GnC_Cy Cuts at: 2587 2587 Size: 5455 EcoRI G'AATT_C Cuts at: 2764 3142 2764 Size: 378 5077 FspI TGC'GCA Cuts at: 2450 3961 2450 Size: 1511 3944 HaeII r_GCGC'y Cuts at: 1678 2136 2311 4602 1678 Size: 458 175 2291 2531 Fragments arranged by size: 2531 2291 458 175 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4096 4128 4170 4202 1373 Size: 32 135 32 2524 32 42 32 2626 Fragments arranged by size: 2626 2524 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2799 3045 669 Size: 988 281 861 246 3079 Fragments arranged by size: 3079 988 861 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5455 HpaI GTT'AAC Cuts at: 3045 3045 Size: 5455 KasI G'GCGC_C Cuts at: 2132 2132 Size: 5455 KpnI G_GTAC'C Cuts at: 2780 2780 Size: 5455 MfeI C'AATT_G Cuts at: 3054 3054 Size: 5455 MluI A'CGCG_T Cuts at: 5331 5331 Size: 5455 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2337 4569 4753 1305 Size: 1032 2232 184 2007 Fragments arranged by size: 2232 2007 1032 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2681 10 Size: 54 1425 343 849 2784 Fragments arranged by size: 2784 1425 849 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2045 2300 2333 2723 2735 3413 3772 Size: 648 879 255 33 390 12 678 359 Cuts at: 3772 3931 518 Size: 159 2042 Fragments arranged by size: 2042 879 678 648 390 359 255 159 33 12 MspA1I CmG'CkG Cuts at: 1606 2173 2871 3548 4696 4941 1606 Size: 567 698 677 1148 245 2120 Fragments arranged by size: 2120 1148 698 677 567 245 NaeI GCC'GGC Cuts at: 2266 2266 Size: 5455 NarI GG'CG_CC Cuts at: 2133 2133 Size: 5455 NcoI C'CATG_G Cuts at: 513 1065 2004 2649 513 Size: 552 939 645 3319 Fragments arranged by size: 3319 939 645 552 NdeI CA'TA_TG Cuts at: 387 2734 387 Size: 2347 3108 NgoMIV G'CCGG_C Cuts at: 2264 2264 Size: 5455 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5455 NotI GC'GGCC_GC Cuts at: 2902 2902 Size: 5455 NspI r_CATG'y Cuts at: 1132 1229 2813 4358 1132 Size: 97 1584 1545 2229 Fragments arranged by size: 2229 1584 1545 97 PasI CC'CwG_GG Cuts at: 1348 2671 2751 1348 Size: 1323 80 4052 Fragments arranged by size: 4052 1323 80 PciI A'CATG_T Cuts at: 4354 4354 Size: 5455 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5455 PmeI GTTT'AAAC Cuts at: 2759 2759 Size: 5455 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3524 3556 5037 5069 3524 Size: 32 1481 32 3910 Fragments arranged by size: 3910 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2660 1613 Size: 1047 4408 PsiI TTA'TAA Cuts at: 3025 3025 Size: 5455 PstI C_TGCA'G Cuts at: 830 2807 830 Size: 1977 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5455 PvuII CAG'CTG Cuts at: 2173 2173 Size: 5455 SacI G_AGCT'C Cuts at: 2622 2622 Size: 5455 SalI G'TCGA_C Cuts at: 1936 2797 1936 Size: 861 4594 SbfI CC_TGCA'GG Cuts at: 2807 2807 Size: 5455 ScaI AGT'ACT Cuts at: 3703 5415 3703 Size: 1712 3743 SfcI C'TryA_G Cuts at: 826 1045 2803 3938 4619 4810 826 Size: 219 1758 1135 681 191 1471 Fragments arranged by size: 1758 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2134 2134 Size: 5455 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5455 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5455 SmaI CCC'GGG Cuts at: 1872 2782 1872 Size: 910 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3559 4460 4722 820 Size: 197 926 1616 901 262 1553 Fragments arranged by size: 1616 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3246 493 Size: 2753 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5455 SphI G_CATG'C Cuts at: 1132 2813 1132 Size: 1681 3774 SspI AAT'ATT Cuts at: 5 52 3379 5 Size: 47 3327 2081 Fragments arranged by size: 3327 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5455 StyI C'CwwG_G Cuts at: 513 1065 2004 2082 2649 2894 513 Size: 552 939 78 567 245 3074 Fragments arranged by size: 3074 939 567 552 245 78 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2165 3482 3499 3652 3837 4282 325 Size: 1255 585 1317 17 153 185 445 1498 Fragments arranged by size: 1498 1317 1255 585 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3701 5413 96 Size: 274 80 33 51 1443 1724 1712 138 Fragments arranged by size: 1724 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2084 2116 5415 5447 2084 Size: 32 3299 32 2092 Fragments arranged by size: 3299 2092 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5455 XbaI T'CTAG_A Cuts at: 2791 2791 Size: 5455 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2368 2656 2689 2368 Size: 288 33 5134 Fragments arranged by size: 5134 288 33 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5455 XmaI C'CCGG_G Cuts at: 1870 2780 1870 Size: 910 4545 XmnI GAAnn'nnTTC Cuts at: 3584 3584 Size: 5455 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2560 3263 276 Size: 53 83 186 936 1026 703 2468 Fragments arranged by size: 2468 1026 936 703 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI