################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BamHI BbeI BbrPI BbvCI BciVI BclI BfaI BfrI BlnI BsaBI BsaWI BseAI BsiWI BsmFI BsoBI Bsp1407I BspDI BspEI BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraII DraIII DrdI Eam1105I Eco47III Eco81I EcoO109I EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SpeI SphI Sse8387I SspBI StuI SwaI Tsp45I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AatII 1956 GACGTC GACGT^C AccI 753 GTCTAC GT^[AC][GT]AC AflIII 1086 ACATGT A^C[GA][CT]GT Alw44I 967 GTGCAC G^TGCAC AlwI 1464 GATCC GGATC(4/5) ApaLI 967 GTGCAC G^TGCAC BcgI 1924 GCAAAGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 1830 GCCTGAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1242 AGATCT A^GATCT BlpI 1035 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1035 GCTCAGC GC^T[ACGT]AGC BsaAI 655 TACGTA [CT]AC^GT[GA] BsiEI 1590 CGGCCG CG[GA][CT]^CG BsmI 617 GCATTC GAATGC(1/-1) BspHI 1731 TCATGA T^CATGA BspLU11I 1086 ACATGT A^CATGT BsrDI 984 CATTGC GCAATG(2/0) CelII 1035 GCTCAGC GC^T[ACGT]AGC DraI 373 TTTAAA TTT^AAA EagI 1590 CGGCCG C^GGCCG EclXI 1590 CGGCCG C^GGCCG Eco52I 1590 CGGCCG C^GGCCG EcoNI 1446 CCTTGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HgaI 1871 GCGTC GACGC(5/10) HphI 142 TCACC GGTGA(8/7) MaeIII 938 GTAAC ^GT[ACGT]AC MslI 1083 CACACATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 1493 CCATGG C^CATGG NspI 1086 ACATGT [GA]CATG^[CT] RcaI 1731 TCATGA T^CATGA SacI 1483 GAGCTC GAGCT^C SapI 1690 GAAGAGC GCTCTTC(1/4) ScaI 1610 AGTACT AGT^ACT SmlI 1876 CTTGAG C^T[CT][GA]AG SnaBI 655 TACGTA TAC^GTA SspI 1438 AATATT AAT^ATT ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AcyI 1870 GGCGTC G[GA]^CG[CT]C 1956 GACGTC AlwNI 464 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 1501 CAGTTTCTG AspI 976 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1867 GACGGCGTC BalI 1154 TGGCCA TGG^CCA 1490 TGGCCA BanI 531 GGTGCC G^G[CT][GA]CC 1846 GGTGCC BanII 136 GAGCCC G[GA]GC[CT]^C 1483 GAGCTC BbsI 766 GAAGAC GAAGAC(2/6) 1669 GAAGAC BcnI 74 CCCGG CC^[GC]GG 744 CCGGG BpmI 1780 CTCCAG CTGGAG(16/14) 278 CTGGAG BpuAI 766 GAAGAC GAAGAC(2/6) 1669 GAAGAC BsaHI 1870 GGCGTC G[GA]^CG[CT]C 1956 GACGTC BsaI 815 GAGACC GGTCTC(1/5) 1177 GAGACC BsmBI 1865 GAGACG CGTCTC(1/5) 1954 GAGACG BsrFI 1511 GCCGGC [GA]^CCGG[CT] 1920 GCCGGC BstAPI 804 GCAGTATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1500 GCAGTTTCTGC BstYI 1242 AGATCT [GA]^GATC[CT] 1463 AGATCC Cfr10I 1511 GCCGGC [GA]^CCGG[CT] 1920 GCCGGC DsaI 881 CCACGG C^C[GA][CT]GG 1493 CCATGG EcoRI 572 GAATTC G^AATTC 904 GAATTC Hin1I 1870 GGCGTC G[GA]^CG[CT]C 1956 GACGTC MaeII 656 ACGT A^CGT 1957 ACGT MflI 1242 AGATCT [GA]^GATC[CT] 1463 AGATCC MluNI 1154 TGGCCA TGG^CCA 1490 TGGCCA MscI 1154 TGGCCA TGG^CCA 1490 TGGCCA NaeI 1511 GCCGGC GCC^GGC 1920 GCCGGC NciI 74 CCCGG CC^[GC]GG 744 CCGGG NgoMIV 1511 GCCGGC G^CCGGC 1920 GCCGGC PflFI 976 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1867 GACGGCGTC PstI 1246 CTGCAG CTGCA^G 1654 CTGCAG PvuII 467 CAGCTG CAG^CTG 1768 CAGCTG TfiI 323 GAATC G^A[AT]TC 1719 GAATC Tth111I 976 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1867 GACGGCGTC XhoII 1242 AGATCT [GA]^GATC[CT] 1463 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AccII AciI AcsI AfaI AluI ApoI AspHI AvaII BbvI BmyI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI Ksp632I MboI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI Tru9I TseI Tsp509I TspRI TthHB8I