################################## # NO CUT ENZYMES ( 174 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AfaI AflII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbrPI BbsI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Cfr10I ClaI CpoI DpnII DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HgaI HincII HindII HindIII HpaI KpnI Ksp632I KspI MaeI MamI MboI MboII MfeI MflI MluI MluNI MroI MscI MseI MunI MvnI NaeI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XcmI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AcyI 152 GGCGCC G[GA]^CG[CT]C AflIII 65 ACACGT A^C[GA][CT]GT AocI 197 CCTCAGG CC^T[ACGT]AGG Asp700I 51 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 338 CTCGAG C^[CT]CG[GA]G BanI 152 GGCGCC G^G[CT][GA]CC BanII 259 GAGCCC G[GA]GC[CT]^C BbeI 152 GGCGCC GGCGC^C BbvCI 129 CCTCAGC CCTCAGC(-5/-2) BlpI 4 GCTGAGC GC^T[ACGT]AGC Bpu1102I 4 GCTGAGC GC^T[ACGT]AGC BsaHI 152 GGCGCC G[GA]^CG[CT]C BsoBI 338 CTCGAG C^[CT]CG[GA]G BsrI 382 ACTGG ACTGG(1/-1) Bsu36I 197 CCTCAGG CC^T[ACGT]AGG CelII 4 GCTGAGC GC^T[ACGT]AGC DsaI 224 CCACGG C^C[GA][CT]GG EaeI 500 TGGCCG [CT]^GGCC[GA] Eco81I 197 CCTCAGG CC^T[ACGT]AGG EcoT14I 357 CCTTGG C^C[AT][AT]GG HaeII 152 GGCGCC [GA]GCGC^[CT] Hin1I 152 GGCGCC G[GA]^CG[CT]C HphI 242 TCACC GGTGA(8/7) KasI 152 GGCGCC G^GCGCC MaeII 67 ACGT A^CGT MslI 231 CACTGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 289 CAGCTG C[AC]G^C[GT]G NarI 152 GGCGCC GG^CGCC PaeR7I 338 CTCGAG C^TCGAG PflMI 539 CCATCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 289 CAGCTG CAG^CTG SfaNI 482 GCATC GCATC(5/9) SfoI 152 GGCGCC GGC^GCC StyI 357 CCTTGG C^C[AT][AT]GG TaqI 339 TCGA T^CGA TthHB8I 339 TCGA T^CGA Van91I 539 CCATCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 338 CTCGAG C^TCGAG XmnI 51 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AccI 343 GTCTAC GT^[AC][GT]AC 417 GTCTAC AluI 23 AGCT AG^CT 290 AGCT BcnI 18 CCGGG CC^[GC]GG 149 CCCGG BpmI 143 CTCCAG CTGGAG(16/14) 256 CTGGAG BstXI 80 CCAAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 185 CCACAGTGCTGG CfoI 153 GCGC GCG^C 330 GCGC HapII 18 CCGG C^CGG 150 CCGG HhaI 153 GCGC GCG^C 330 GCGC HinP1I 153 GCGC G^CGC 330 GCGC HinfI 141 GACTC G^A[ACGT]TC 341 GAGTC HpaII 18 CCGG C^CGG 150 CCGG MspI 18 CCGG C^CGG 150 CCGG NciI 18 CCGGG CC^[GC]GG 149 CCCGG NlaIII 237 CATG CATG^ 268 CATG PleI 141 GACTC GAGTC(4/5) 341 GAGTC ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AciI AvaII BbvI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I DdeI EcoRII Fnu4HI FokI HaeIII ItaI MaeIII MnlI MvaI MwoI NlaIV Sau96I ScrFI SmlI TseI Tsp45I TspRI