################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI AocI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I CelII ClaI CpoI DsaI EagI Eam1105I EclXI Eco52I Eco81I EcoT22I FbaI FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NarI NcoI NdeI NotI NruI NsiI NspI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Alw44I 878 GTGCAC G^TGCAC ApaI 365 GGGCCC GGGCC^C ApaLI 878 GTGCAC G^TGCAC BalI 1469 TGGCCA TGG^CCA BamHI 777 GGATCC G^GATCC BglI 490 GCCACCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 1600 TACGTG [CT]AC^GT[GA] BseRI 1435 GAGGAG GAGGAG(10/8) BspMI 171 ACCTGC ACCTGC(4/8) BsrBI 424 GAGCGG CCGCTC(-3/-3) BsrFI 1283 GCCGGC [GA]^CCGG[CT] BstEII 990 GGTGACC G^GT[ACGT]ACC BstPI 990 GGTGACC G^GT[ACGT]ACC BstYI 777 GGATCC [GA]^GATC[CT] Cfr10I 1283 GCCGGC [GA]^CCGG[CT] DraI 391 TTTAAA TTT^AAA DraII 1145 GGGACCC [GA]G^G[ACGT]CC[CT] DrdI 790 GACTCCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 699 CCTGTCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1145 GGGACCC [GA]G^G[ACGT]CC[CT] EcoO65I 990 GGTGACC G^GT[ACGT]ACC EcoRI 847 GAATTC G^AATTC EcoRV 481 GATATC GAT^ATC HincII 1084 GTTGAC GT[CT]^[GA]AC HindII 1084 GTTGAC GT[CT]^[GA]AC MflI 777 GGATCC [GA]^GATC[CT] MluNI 1469 TGGCCA TGG^CCA MscI 1469 TGGCCA TGG^CCA MseI 392 TTAA T^TAA NaeI 1283 GCCGGC GCC^GGC NgoMIV 1283 GCCGGC G^CCGGC NheI 1312 GCTAGC G^CTAGC PaeR7I 40 CTCGAG C^TCGAG PpuMI 1145 GGGACCC [GA]G^G[AT]CC[CT] PstI 580 CTGCAG CTGCA^G SacI 1365 GAGCTC GAGCT^C SapI 1372 GAAGAGC GCTCTTC(1/4) ScaI 311 AGTACT AGT^ACT SfcI 580 CTGCAG C^T[GA][CT]AG TfiI 225 GATTC G^A[AT]TC Tru9I 392 TTAA T^TAA XcmI 494 CCAAGGCTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 40 CTCGAG C^TCGAG XhoII 777 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AfaI 220 GTAC GT^AC 312 GTAC AlwNI 373 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 1238 CAGAAGCTG Aor51HI 584 AGCGCT AGC^GCT 956 AGCGCT BfaI 472 CTAG C^TAG 1313 CTAG BsaI 168 GAGACC GGTCTC(1/5) 596 GAGACC BsgI 811 CTGCAC GTGCAG(16/14) 1213 GTGCAG BstXI 671 CCACCCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 737 CCAAGTGCTTGG DraIII 51 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG 1462 CACTGTGTG EarI 1372 GAAGAG CTCTTC(1/4) 1387 GAAGAG Eco47III 584 AGCGCT AGC^GCT 956 AGCGCT Ksp632I 1372 GAAGAG CTCTTC(1/4) 1387 GAAGAG MaeI 472 CTAG C^TAG 1313 CTAG MslI 105 CACCGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 753 CATGAAGGTG PflMI 648 CCAAGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 953 CCAAGCGCTGG PvuII 376 CAGCTG CAG^CTG 397 CAGCTG RsaI 220 GTAC GT^AC 312 GTAC SmlI 40 CTCGAG C^T[CT][GA]AG 298 CTTGAG Van91I 648 CCAAGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 953 CCAAGCGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EaeI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PshAI Sau3AI Sau96I ScrFI SfaNI SmaI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I XmaCI XmaI