(Circular) (Six-base) MAPSORT of: pFN21AE3934.seq Check: 9175 from: 1 to: 6340 pFN21AE3934 6340 bp With 182 enzymes: SgfI * March 28, 2012 11:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4150 278 Size: 53 83 186 936 2614 2468 Fragments arranged by size: 2614 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3683 1937 Size: 1746 4594 Acc65I G'GTAC_C Cuts at: 3661 3661 Size: 6340 AclI AA'CG_TT Cuts at: 1563 4467 4840 1563 Size: 2904 373 3063 Fragments arranged by size: 3063 2904 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2848 3062 3396 4403 5787 6055 1632 Size: 1216 214 334 1007 1384 268 1917 Fragments arranged by size: 1917 1384 1216 1007 334 268 214 AfeI AGC'GCT Cuts at: 2591 2963 2591 Size: 372 5968 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6143 AflIII A'CryG_T Cuts at: 1203 5239 6216 1203 Size: 4036 977 1327 Fragments arranged by size: 4036 1327 977 AgeI A'CCGG_T Cuts at: 5171 5171 Size: 6340 AhdI GACnn_n'nnGTC Cuts at: 5069 5069 Size: 6340 AleI CACnn'nnGTG Cuts at: 1166 2114 1166 Size: 948 5392 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6340 AlwNI CAG_nnn'CTG Cuts at: 2383 3248 4083 5655 2383 Size: 865 835 1572 3068 Fragments arranged by size: 3068 1572 865 835 ApaI G_GGCC'C Cuts at: 2374 2374 Size: 6340 ApaLI G'TGCA_C Cuts at: 2883 4397 5553 2883 Size: 1514 1156 3670 Fragments arranged by size: 3670 1514 1156 ApoI r'AATT_y Cuts at: 1493 2201 2423 2852 3649 3875 4027 6136 1493 Size: 708 222 429 797 226 152 2109 1697 Fragments arranged by size: 2109 1697 797 708 429 226 222 152 AseI AT'TA_AT Cuts at: 160 1032 4894 160 Size: 872 3862 1606 Fragments arranged by size: 3862 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6340 AvaI C'yCGr_G Cuts at: 1870 1943 2045 2839 2941 3573 3665 1870 Size: 73 102 794 102 632 92 4545 Fragments arranged by size: 4545 794 632 102 102 92 73 BamHI G'GATC_C Cuts at: 2782 3670 4037 2782 Size: 888 367 5085 Fragments arranged by size: 5085 888 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2687 2728 2906 3661 5116 Size: 325 217 627 900 41 178 755 1455 Cuts at: 5116 618 Size: 1842 Fragments arranged by size: 1842 1455 900 755 627 325 217 178 41 BanII G_rGCy'C Cuts at: 1391 2374 2703 3171 3374 3659 1391 Size: 983 329 468 203 285 4072 Fragments arranged by size: 4072 983 468 329 285 203 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3140 3537 3558 928 Size: 971 1241 397 21 3710 Fragments arranged by size: 3710 1241 971 397 21 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4531 4565 1903 Size: 34 2594 34 3678 Fragments arranged by size: 3678 2594 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4232 5442 4232 Size: 1210 5130 BclI T'GATC_A Cuts at: 1548 1548 Size: 6340 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2501 4951 136 Size: 107 122 71 631 605 829 2450 1525 Fragments arranged by size: 2450 1525 829 631 605 122 107 71 BglII A'GATC_T Cuts at: 6335 6335 Size: 6340 BlpI GC'TnA_GC Cuts at: 3757 3757 Size: 6340 Bme1580I G_kGCm'C Cuts at: 1790 2018 2374 2733 2887 2909 3509 3612 4401 Size: 228 356 359 154 22 600 103 789 Cuts at: 4401 5557 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 789 600 359 356 228 154 103 22 BmgBI CAC'GTC Cuts at: 1318 2803 2848 1318 Size: 1485 45 4810 Fragments arranged by size: 4810 1485 45 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4071 5029 442 Size: 416 53 83 399 24 2654 958 1753 Fragments arranged by size: 2654 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3321 1056 Size: 2265 4075 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3403 3650 3403 Size: 247 6093 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2219 2426 2741 5000 1239 Size: 980 207 315 2259 2579 Fragments arranged by size: 2579 2259 980 315 207 Bpu10I CC'TnA_GC Cuts at: 3188 3263 3284 3188 Size: 75 21 6244 Fragments arranged by size: 6244 75 21 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2324 4465 5330 5628 2324 Size: 2141 865 298 3036 Fragments arranged by size: 3036 2141 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2167 2595 5003 882 Size: 617 668 428 2408 2219 Fragments arranged by size: 2408 2219 668 617 428 BsaAI yAC'GTr Cuts at: 493 1204 3607 4131 493 Size: 711 2403 524 2702 Fragments arranged by size: 2702 2403 711 524 BsaBI GATnn'nnATC Cuts at: 6334 6334 Size: 6340 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4147 4529 5164 275 Size: 53 83 186 936 2614 382 635 1451 Fragments arranged by size: 2614 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4773 5171 5445 5592 6003 4773 Size: 398 274 147 411 5110 Fragments arranged by size: 5110 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2217 2247 1178 Size: 30 165 30 814 30 5271 Fragments arranged by size: 5271 814 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3455 1186 Size: 2269 4071 BseYI C'CCAG_C Cuts at: 2373 5543 6039 2373 Size: 3170 496 2674 Fragments arranged by size: 3170 2674 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2801 3239 1163 Size: 573 1065 438 4264 Fragments arranged by size: 4264 1065 573 438 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3790 4551 5579 1540 Size: 275 186 1789 761 1028 2301 Fragments arranged by size: 2301 1789 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2046 2887 3374 4401 4486 5557 2046 Size: 841 487 1027 85 1071 2829 Fragments arranged by size: 2829 1071 1027 841 487 85 BsmI GAATG_Cn' Cuts at: 3851 3944 3851 Size: 93 6247 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2018 2046 2374 2703 2733 2887 2909 Size: 399 228 28 328 329 30 154 22 Cuts at: 2909 3171 3374 3509 3612 3659 4401 4486 5557 Size: 262 203 135 103 47 742 85 1071 Cuts at: 5557 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 329 328 262 228 203 154 135 103 85 47 30 28 22 BspEI T'CCGG_A Cuts at: 6003 6003 Size: 6340 BspHI T'CATG_A Cuts at: 4229 5959 4229 Size: 1730 4610 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2185 3695 844 Size: 1341 1510 3489 Fragments arranged by size: 3489 1510 1341 BsrBI CCG'CTC Cuts at: 1394 1823 2431 4227 1394 Size: 429 608 1796 3507 Fragments arranged by size: 3507 1796 608 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4835 5009 65 Size: 1183 3587 174 1396 Fragments arranged by size: 3587 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3288 4984 5171 1700 Size: 110 1478 1696 187 2869 Fragments arranged by size: 2869 1696 1478 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6340 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6340 BssSI C'ACGA_G Cuts at: 4400 5412 4400 Size: 1012 5328 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6340 BstBI TT'CG_AA Cuts at: 5144 5144 Size: 6340 BstEII G'GTnAC_C Cuts at: 1184 2995 1184 Size: 1811 4529 BstXI CCAn_nnnn'nTGG Cuts at: 2683 2749 2683 Size: 66 6274 BstYI r'GATC_y Cuts at: 1972 2782 3670 4037 4422 4439 5891 5977 5989 Size: 810 888 367 385 17 1452 86 12 Cuts at: 5989 6335 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 888 810 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4849 Fragments arranged by size: 4849 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5601 Fragments arranged by size: 5601 683 56 BtsI GCAGTG_nn' Cuts at: 2066 3856 4650 4670 2066 Size: 1790 794 20 3736 Fragments arranged by size: 3736 1790 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6340 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2760 2795 529 Size: 35 2196 35 4074 Fragments arranged by size: 4074 2196 35 35 DraI TTT'AAA Cuts at: 2398 3644 2398 Size: 1246 5094 DraIII CAC_nnn'GTG Cuts at: 2061 3472 2061 Size: 1411 4929 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2801 5347 809 Size: 507 1485 2546 1802 Fragments arranged by size: 2546 1802 1485 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2550 3474 3519 Size: 54 1425 50 275 18 720 924 45 Cuts at: 3519 3787 4676 6305 8 Size: 268 889 1629 43 Fragments arranged by size: 1629 1425 924 889 720 275 268 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3787 1537 Size: 275 1975 4090 Fragments arranged by size: 4090 1975 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3372 3387 4270 1353 Size: 2019 15 883 3423 Fragments arranged by size: 3423 2019 883 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3598 4927 5301 5447 387 Size: 1064 138 2009 1329 374 146 1280 Fragments arranged by size: 2009 1329 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3372 3372 Size: 6340 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2219 2426 2741 2848 3062 3396 4403 Size: 393 587 207 315 107 214 334 1007 Cuts at: 4403 5000 5787 6055 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1007 787 597 587 393 334 315 268 214 207 107 EcoNI CCTnn'n_nnAGG Cuts at: 2708 2708 Size: 6340 EcoO109I rG'GnC_Cy Cuts at: 3151 3151 Size: 6340 EcoRI G'AATT_C Cuts at: 2852 3649 4027 2852 Size: 797 378 5165 Fragments arranged by size: 5165 797 378 EcoRV GAT'ATC Cuts at: 2488 2488 Size: 6340 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2368 2552 2368 Size: 184 6156 FspI TGC'GCA Cuts at: 4846 4846 Size: 6340 HaeII r_GCGC'y Cuts at: 1678 2053 2593 2965 3324 5487 1678 Size: 375 540 372 359 2163 2531 Fragments arranged by size: 2531 2163 540 375 372 359 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2180 2212 4981 5013 5055 Size: 32 135 32 608 32 2769 32 42 Cuts at: 5055 5087 1373 Size: 32 2626 Fragments arranged by size: 2769 2626 608 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3091 3684 3930 669 Size: 988 281 1153 593 246 3079 Fragments arranged by size: 3079 1153 988 593 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6340 HpaI GTT'AAC Cuts at: 3930 3930 Size: 6340 KpnI G_GTAC'C Cuts at: 3665 3665 Size: 6340 MfeI C'AATT_G Cuts at: 3939 3939 Size: 6340 MluI A'CGCG_T Cuts at: 6216 6216 Size: 6340 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2058 2688 2916 3036 5454 5638 1305 Size: 753 630 228 120 2418 184 2007 Fragments arranged by size: 2418 2007 753 630 228 184 120 MscI TGG'CCA Cuts at: 10 64 1489 1832 3476 10 Size: 54 1425 343 1644 2874 Fragments arranged by size: 2874 1644 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2114 2762 4298 4657 4816 518 Size: 648 948 648 1536 359 159 2042 Fragments arranged by size: 2042 1536 948 648 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2263 2383 2404 3756 4433 5581 5826 1606 Size: 657 120 21 1352 677 1148 245 2120 Fragments arranged by size: 2120 1352 1148 677 657 245 120 21 NaeI GCC'GGC Cuts at: 3290 3290 Size: 6340 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4849 Fragments arranged by size: 4849 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6340 NgoMIV G'CCGG_C Cuts at: 3288 3288 Size: 6340 NheI G'CTAG_C Cuts at: 1052 3317 1052 Size: 2265 4075 NotI GC'GGCC_GC Cuts at: 3787 3787 Size: 6340 NspI r_CATG'y Cuts at: 1132 1229 3698 5243 1132 Size: 97 2469 1545 2229 Fragments arranged by size: 2469 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6340 PciI A'CATG_T Cuts at: 5239 5239 Size: 6340 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2659 2964 1108 Size: 902 649 305 4484 Fragments arranged by size: 4484 902 649 305 PfoI T'CCnGG_A Cuts at: 2235 2235 Size: 6340 PmeI GTTT'AAAC Cuts at: 3644 3644 Size: 6340 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4409 4441 5922 5954 4409 Size: 32 1481 32 4795 Fragments arranged by size: 4795 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3151 3151 Size: 6340 PshAI GACnn'nnGTC Cuts at: 1613 2898 3537 3575 1613 Size: 1285 639 38 4378 Fragments arranged by size: 4378 1285 639 38 PsiI TTA'TAA Cuts at: 3910 3910 Size: 6340 PspOMI G'GGCC_C Cuts at: 2370 2370 Size: 6340 PspXI vC'TCGA_Gb Cuts at: 2045 2045 Size: 6340 PstI C_TGCA'G Cuts at: 830 2589 3692 830 Size: 1759 1103 3478 Fragments arranged by size: 3478 1759 1103 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6340 PvuII CAG'CTG Cuts at: 2383 2404 2383 Size: 21 6319 SacI G_AGCT'C Cuts at: 3374 3374 Size: 6340 SalI G'TCGA_C Cuts at: 1936 3682 1936 Size: 1746 4594 SanDI GG'GwC_CC Cuts at: 3151 3151 Size: 6340 SapI GCTCTTCn'nnn_ Cuts at: 3372 3372 Size: 6340 SbfI CC_TGCA'GG Cuts at: 3692 3692 Size: 6340 ScaI AGT'ACT Cuts at: 2318 4588 6300 2318 Size: 2270 1712 2358 Fragments arranged by size: 2358 2270 1712 SfcI C'TryA_G Cuts at: 826 1045 2585 3688 4823 5504 5695 826 Size: 219 1540 1103 1135 681 191 1471 Fragments arranged by size: 1540 1471 1135 1103 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6340 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6340 SmaI CCC'GGG Cuts at: 1872 2841 2943 3575 3667 1872 Size: 969 102 632 92 4545 Fragments arranged by size: 4545 969 632 102 92 SmlI C'TyrA_G Cuts at: 820 1017 1943 2045 2303 4444 5345 5607 820 Size: 197 926 102 258 2141 901 262 1553 Fragments arranged by size: 2141 1553 926 901 262 258 197 102 SnaBI TAC'GTA Cuts at: 493 4131 493 Size: 3638 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6340 SphI G_CATG'C Cuts at: 1132 3698 1132 Size: 2566 3774 SrfI GCCC'GGGC Cuts at: 2841 2841 Size: 6340 SspI AAT'ATT Cuts at: 5 52 4264 5 Size: 47 4212 2081 Fragments arranged by size: 4212 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6340 StyI C'CwwG_G Cuts at: 513 1065 2004 2181 2499 3363 3779 513 Size: 552 939 177 318 864 416 3074 Fragments arranged by size: 3074 939 864 552 416 318 177 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2675 3014 4367 4384 4537 4722 5167 Size: 1255 1095 339 1353 17 153 185 445 Cuts at: 5167 325 Size: 1498 Fragments arranged by size: 1498 1353 1255 1095 445 339 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2316 4586 6298 Size: 274 80 33 51 1443 339 2270 1712 Cuts at: 6298 96 Size: 138 Fragments arranged by size: 2270 1712 1443 339 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6300 6332 6300 Size: 32 6308 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6340 XbaI T'CTAG_A Cuts at: 3676 3676 Size: 6340 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2506 2506 Size: 6340 XhoI C'TCGA_G Cuts at: 1943 2045 1943 Size: 102 6238 XmaI C'CCGG_G Cuts at: 1870 2839 2941 3573 3665 1870 Size: 969 102 632 92 4545 Fragments arranged by size: 4545 969 632 102 92 XmnI GAAnn'nnTTC Cuts at: 4469 4469 Size: 6340 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4148 276 Size: 53 83 186 936 2614 2468 Fragments arranged by size: 2614 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I FseI FspAI KasI NarI NruI NsiI PacI PmlI PsrI RsrII SacII SexAI SfiI SfoI SwaI