################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII Acc65I AclI AflII AflIII AgeI AhdI AlwI Aor51HI ApaI AscI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanII BbeI BbvCI BcgI BciVI BfaI BfrI BglII BlnI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstYI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoT22I FauI FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI KspI MaeI MfeI MflI MluI MslI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccI 1095 GTATAC GT^[AC][GT]AC AccIII 1082 TCCGGA T^CCGGA AcsI 634 GAATTC [GA]^AATT[CT] AcyI 148 GGCGTC G[GA]^CG[CT]C Alw44I 682 GTGCAC G^TGCAC AocI 107 CCTCAGG CC^T[ACGT]AGG ApaLI 682 GTGCAC G^TGCAC ApoI 634 GAATTC [GA]^AATT[CT] AvaI 885 CCCGGG C^[CT]CG[GA]G BalI 92 TGGCCA TGG^CCA BanI 1192 GGCACC G^G[CT][GA]CC BbrPI 58 CACGTG CAC^GTG BclI 470 TGATCA T^GATCA BglI 94 GCCAAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 874 GCTGAGC GC^T[ACGT]AGC Bpu1102I 874 GCTGAGC GC^T[ACGT]AGC BsaAI 58 CACGTG [CT]AC^GT[GA] BsaBI 1074 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 148 GGCGTC G[GA]^CG[CT]C BsaI 376 GAGACC GGTCTC(1/5) BsaWI 1082 TCCGGA [AT]^CCGG[AT] BseAI 1082 TCCGGA T^CCGGA BsoBI 885 CCCGGG C^[CT]CG[GA]G BspEI 1082 TCCGGA T^CCGGA BsrBI 84 GAGCGG CCGCTC(-3/-3) BsrDI 870 CATTGC GCAATG(2/0) Bst1107I 1095 GTATAC GTA^TAC BstXI 1331 CCAAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 1095 GTATAC GTA^TAC Bsu36I 107 CCTCAGG CC^T[ACGT]AGG CelII 874 GCTGAGC GC^T[ACGT]AGC DsaI 437 CCATGG C^C[GA][CT]GG Eco81I 107 CCTCAGG CC^T[ACGT]AGG EcoRI 634 GAATTC G^AATTC EcoRV 763 GATATC GAT^ATC FbaI 470 TGATCA T^GATCA Hin1I 148 GGCGTC G[GA]^CG[CT]C MaeII 59 ACGT A^CGT MamI 1074 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 92 TGGCCA TGG^CCA MroI 1082 TCCGGA T^CCGGA MscI 92 TGGCCA TGG^CCA MspA1I 1222 CAGCTG C[AC]G^C[GT]G NcoI 437 CCATGG C^CATGG PflMI 912 CCACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 58 CACGTG CAC^GTG PmlI 58 CACGTG CAC^GTG PvuII 1222 CAGCTG CAG^CTG SmaI 885 CCCGGG CCC^GGG Van91I 912 CCACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 885 CCCGGG C^CCGGG XmaI 885 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AlwNI 253 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG 1222 CAGCTGCTG AseI 162 ATTAAT AT^TAAT 835 ATTAAT AsnI 162 ATTAAT AT^TAAT 835 ATTAAT AspHI 21 GAGCAC G[AT]GC[AT]^C 682 GTGCAC BpmI 560 CTGGAG CTGGAG(16/14) 955 CTGGAG BseRI 10 GAGGAG GAGGAG(10/8) 1162 GAGGAG BsgI 943 CTGCAC GTGCAG(16/14) 1219 GTGCAG BsiHKAI 21 GAGCAC G[AT]GC[AT]^C 682 GTGCAC BsmAI 376 GAGAC GTCTC(1/5) 424 GAGAC BspMI 941 ACCTGC ACCTGC(4/8) 611 GCAGGT BsrI 220 ACTGG ACTGG(1/-1) 513 ACTGG DraII 207 AGGACCT [GA]G^G[ACGT]CC[CT] 269 AGGTCCC EaeI 92 TGGCCA [CT]^GGCC[GA] 1235 CGGCCA EcoO109I 207 AGGACCT [GA]G^G[ACGT]CC[CT] 269 AGGTCCC EcoT14I 380 CCAAGG C^C[AT][AT]GG 437 CCATGG HgaI 33 GACGC GACGC(5/10) 149 GCGTC MaeIII 403 GTCAC ^GT[ACGT]AC 712 GTGAC PleI 479 GACTC GAGTC(4/5) 958 GAGTC PpuMI 207 AGGACCT [GA]G^G[AT]CC[CT] 269 AGGTCCC PshBI 162 ATTAAT AT^TAAT 835 ATTAAT SapI 18 GAAGAGC GCTCTTC(1/4) 188 GAAGAGC StyI 380 CCAAGG C^C[AT][AT]GG 437 CCATGG TfiI 432 GAATC G^A[AT]TC 1148 GAATC Tsp45I 403 GTCAC ^GT[GC]AC 712 GTGAC Tsp509I 161 AATT ^AATT 635 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AccII AciI AfaI AluI AvaII BbsI BbvI BcnI BmyI BpuAI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MboI MboII MnlI MseI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI RsaI Sau3AI Sau96I ScrFI SfaNI TaqI Tru9I TseI TspRI TthHB8I