(Circular) (Six-base) MAPSORT of: pFN21AE1123.seq Check: 2994 from: 1 to: 6073 pFN21AE1123 6073 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2607 2607 Size: 6073 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3883 278 Size: 53 83 186 936 2347 2468 Fragments arranged by size: 2468 2347 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3101 3416 1937 Size: 1164 315 4594 Fragments arranged by size: 4594 1164 315 Acc65I G'GTAC_C Cuts at: 3394 3394 Size: 6073 AclI AA'CG_TT Cuts at: 1563 4200 4573 1563 Size: 2637 373 3063 Fragments arranged by size: 3063 2637 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2676 4136 5520 5788 1632 Size: 1044 1460 1384 268 1917 Fragments arranged by size: 1917 1460 1384 1044 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5876 AflIII A'CryG_T Cuts at: 1203 4972 5949 1203 Size: 3769 977 1327 Fragments arranged by size: 3769 1327 977 AgeI A'CCGG_T Cuts at: 4904 4904 Size: 6073 AhdI GACnn_n'nnGTC Cuts at: 4802 4802 Size: 6073 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6073 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6073 AlwNI CAG_nnn'CTG Cuts at: 2263 3232 3816 5388 2263 Size: 969 584 1572 2948 Fragments arranged by size: 2948 1572 969 584 ApaLI G'TGCA_C Cuts at: 2687 4130 5286 2687 Size: 1443 1156 3474 Fragments arranged by size: 3474 1443 1156 ApoI r'AATT_y Cuts at: 1493 2639 3382 3608 3760 5869 1493 Size: 1146 743 226 152 2109 1697 Fragments arranged by size: 2109 1697 1146 743 226 152 AseI AT'TA_AT Cuts at: 160 1032 2168 2841 4627 160 Size: 872 1136 673 1786 1606 Fragments arranged by size: 1786 1606 1136 872 673 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6073 AvaI C'yCGr_G Cuts at: 1870 1943 2890 3398 1870 Size: 73 947 508 4545 Fragments arranged by size: 4545 947 508 73 BamHI G'GATC_C Cuts at: 3403 3770 3403 Size: 367 5706 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3197 3394 4849 618 Size: 325 217 627 1410 197 1455 1842 Fragments arranged by size: 1842 1455 1410 627 325 217 197 BanII G_rGCy'C Cuts at: 1391 3392 1391 Size: 2001 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2042 2333 2813 2958 928 Size: 971 143 291 480 145 4043 Fragments arranged by size: 4043 971 480 291 145 143 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4264 4298 1903 Size: 34 2327 34 3678 Fragments arranged by size: 3678 2327 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3965 5175 3965 Size: 1210 4863 BclI T'GATC_A Cuts at: 1548 2475 1548 Size: 927 5146 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2105 4684 136 Size: 107 122 71 631 605 433 2579 1525 Fragments arranged by size: 2579 1525 631 605 433 122 107 71 BglII A'GATC_T Cuts at: 6068 6068 Size: 6073 BlpI GC'TnA_GC Cuts at: 2880 3490 2880 Size: 610 5463 Bme1580I G_kGCm'C Cuts at: 1790 2691 3142 4134 5290 1790 Size: 901 451 992 1156 2573 Fragments arranged by size: 2573 1156 992 901 451 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6073 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3804 4762 442 Size: 416 53 83 399 24 2387 958 1753 Fragments arranged by size: 2387 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6073 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2586 2981 4733 1239 Size: 1347 395 1752 2579 Fragments arranged by size: 2579 1752 1347 395 Bpu10I CC'TnA_GC Cuts at: 2256 2256 Size: 6073 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4198 5063 5361 4198 Size: 865 298 4910 Fragments arranged by size: 4910 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2375 4736 882 Size: 617 876 2361 2219 Fragments arranged by size: 2361 2219 876 617 BsaAI yAC'GTr Cuts at: 493 1204 2065 3864 493 Size: 711 861 1799 2702 Fragments arranged by size: 2702 1799 861 711 BsaBI GATnn'nnATC Cuts at: 3083 6067 3083 Size: 2984 3089 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2154 3880 4262 4897 Size: 53 83 186 936 621 1726 382 635 Cuts at: 4897 275 Size: 1451 Fragments arranged by size: 1726 1451 936 635 621 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3087 4506 4904 5178 5325 5736 3087 Size: 1419 398 274 147 411 3424 Fragments arranged by size: 3424 1419 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5848 Fragments arranged by size: 5848 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2030 3182 1186 Size: 844 1152 4077 Fragments arranged by size: 4077 1152 844 BseYI C'CCAG_C Cuts at: 2074 2263 2525 5276 5772 2074 Size: 189 262 2751 496 2375 Fragments arranged by size: 2751 2375 496 262 189 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2933 3245 1163 Size: 573 1197 312 3991 Fragments arranged by size: 3991 1197 573 312 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3523 4284 5312 1540 Size: 275 186 1522 761 1028 2301 Fragments arranged by size: 2301 1522 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2030 2691 4134 4219 5290 2030 Size: 661 1443 85 1071 2813 Fragments arranged by size: 2813 1443 1071 661 85 BsmI GAATG_Cn' Cuts at: 3584 3677 3584 Size: 93 5980 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2030 2691 3142 3392 4134 4219 5290 Size: 399 240 661 451 250 742 85 1071 Cuts at: 5290 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 661 451 399 250 240 85 BspEI T'CCGG_A Cuts at: 3087 5736 3087 Size: 2649 3424 BspHI T'CATG_A Cuts at: 3962 5692 3962 Size: 1730 4343 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2607 2955 3428 844 Size: 1763 348 473 3489 Fragments arranged by size: 3489 1763 473 348 BsrBI CCG'CTC Cuts at: 1394 1823 2091 3960 1394 Size: 429 268 1869 3507 Fragments arranged by size: 3507 1869 429 268 BsrDI GCAATG_nn' Cuts at: 65 1248 2874 4568 4742 65 Size: 1183 1626 1694 174 1396 Fragments arranged by size: 1694 1626 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4717 4904 1700 Size: 110 2907 187 2869 Fragments arranged by size: 2907 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6073 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6073 BssSI C'ACGA_G Cuts at: 4133 5145 4133 Size: 1012 5061 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6073 BstBI TT'CG_AA Cuts at: 4877 4877 Size: 6073 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6073 BstXI CCAn_nnnn'nTGG Cuts at: 3343 3343 Size: 6073 BstYI r'GATC_y Cuts at: 1972 3403 3770 4155 4172 5624 5710 5722 6068 Size: 1431 367 385 17 1452 86 12 346 Cuts at: 6068 1972 Size: 1977 Fragments arranged by size: 1977 1452 1431 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3102 3102 Size: 6073 Bsu36I CC'TnA_GG Cuts at: 2113 2113 Size: 6073 BtgI C'CryG_G Cuts at: 513 1065 2442 513 Size: 552 1377 4144 Fragments arranged by size: 4144 1377 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2032 487 Size: 683 56 806 4528 Fragments arranged by size: 4528 806 683 56 BtsI GCAGTG_nn' Cuts at: 3589 4383 4403 3589 Size: 794 20 5259 Fragments arranged by size: 5259 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6073 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6038 DraI TTT'AAA Cuts at: 3377 3377 Size: 6073 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5080 809 Size: 507 3764 1802 Fragments arranged by size: 3764 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2097 3240 3520 Size: 54 1425 50 275 18 267 1143 280 Cuts at: 3520 4409 6038 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1143 889 280 275 267 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3520 1537 Size: 275 1708 4090 Fragments arranged by size: 4090 1708 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2018 2188 2600 2652 3291 4003 1353 Size: 665 170 412 52 639 712 3423 Fragments arranged by size: 3423 712 665 639 412 170 52 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3322 4660 5034 5180 387 Size: 1064 138 1733 1338 374 146 1280 Fragments arranged by size: 1733 1338 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2586 2676 2981 4136 4733 5520 5788 Size: 393 954 90 305 1155 597 787 268 Cuts at: 5788 1239 Size: 1524 Fragments arranged by size: 1524 1155 954 787 597 393 305 268 90 EcoO109I rG'GnC_Cy Cuts at: 2213 2275 2213 Size: 62 6011 EcoRI G'AATT_C Cuts at: 2639 3382 3760 2639 Size: 743 378 4952 Fragments arranged by size: 4952 743 378 EcoRV GAT'ATC Cuts at: 2770 2770 Size: 6073 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2296 2296 Size: 6073 FspI TGC'GCA Cuts at: 4579 4579 Size: 6073 HaeII r_GCGC'y Cuts at: 1678 5220 1678 Size: 3542 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2422 2454 2754 2759 2786 Size: 32 135 32 850 32 300 5 27 Cuts at: 2786 2791 3023 3055 3311 3343 4714 4746 4788 Size: 5 232 32 256 32 1371 32 42 Cuts at: 4788 4820 1373 Size: 32 2626 Fragments arranged by size: 2626 1371 850 300 256 232 135 42 32 32 32 32 32 32 32 27 5 5 HincII GTy'rAC Cuts at: 669 1657 1938 3417 3663 669 Size: 988 281 1479 246 3079 Fragments arranged by size: 3079 1479 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6073 HpaI GTT'AAC Cuts at: 3663 3663 Size: 6073 KpnI G_GTAC'C Cuts at: 3398 3398 Size: 6073 MfeI C'AATT_G Cuts at: 3672 3672 Size: 6073 MluI A'CGCG_T Cuts at: 5949 5949 Size: 6073 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3021 5187 5371 1305 Size: 1716 2166 184 2007 Fragments arranged by size: 2166 2007 1716 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2099 10 Size: 54 1425 343 267 3984 Fragments arranged by size: 3984 1425 343 267 54 MslI CAynn'nnrTG Cuts at: 518 1166 4031 4390 4549 518 Size: 648 2865 359 159 2042 Fragments arranged by size: 2865 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3229 3489 4166 5314 5559 1606 Size: 1623 260 677 1148 245 2120 Fragments arranged by size: 2120 1623 1148 677 260 245 NcoI C'CATG_G Cuts at: 513 1065 2442 513 Size: 552 1377 4144 Fragments arranged by size: 4144 1377 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6073 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6073 NotI GC'GGCC_GC Cuts at: 3520 3520 Size: 6073 NspI r_CATG'y Cuts at: 1132 1229 2151 2923 3293 3431 4976 1132 Size: 97 922 772 370 138 1545 2229 Fragments arranged by size: 2229 1545 922 772 370 138 97 PasI CC'CwG_GG Cuts at: 1348 2755 1348 Size: 1407 4666 PciI A'CATG_T Cuts at: 4972 4972 Size: 6073 PflMI CCAn_nnn'nTGG Cuts at: 1108 2923 1108 Size: 1815 4258 PfoI T'CCnGG_A Cuts at: 2218 2218 Size: 6073 PmeI GTTT'AAAC Cuts at: 3377 3377 Size: 6073 PmlI CAC'GTG Cuts at: 2065 2065 Size: 6073 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4142 4174 5655 5687 4142 Size: 32 1481 32 4528 Fragments arranged by size: 4528 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2213 2275 2213 Size: 62 6011 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6073 PsiI TTA'TAA Cuts at: 3643 3643 Size: 6073 PstI C_TGCA'G Cuts at: 830 3425 830 Size: 2595 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6073 PvuII CAG'CTG Cuts at: 3229 3229 Size: 6073 SalI G'TCGA_C Cuts at: 1936 3415 1936 Size: 1479 4594 SapI GCTCTTCn'nnn_ Cuts at: 2018 2188 2018 Size: 170 5903 SbfI CC_TGCA'GG Cuts at: 3425 3425 Size: 6073 ScaI AGT'ACT Cuts at: 4321 6033 4321 Size: 1712 4361 SfcI C'TryA_G Cuts at: 826 1045 3421 4556 5237 5428 826 Size: 219 2376 1135 681 191 1471 Fragments arranged by size: 2376 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6073 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6073 SmaI CCC'GGG Cuts at: 1872 2892 3400 1872 Size: 1020 508 4545 Fragments arranged by size: 4545 1020 508 SmlI C'TyrA_G Cuts at: 820 1017 1943 4177 5078 5340 820 Size: 197 926 2234 901 262 1553 Fragments arranged by size: 2234 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3864 493 Size: 3371 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6073 SphI G_CATG'C Cuts at: 1132 3431 1132 Size: 2299 3774 SspI AAT'ATT Cuts at: 5 52 3997 5 Size: 47 3945 2081 Fragments arranged by size: 3945 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6073 StyI C'CwwG_G Cuts at: 513 1065 2385 2442 3512 513 Size: 552 1320 57 1070 3074 Fragments arranged by size: 3074 1320 1070 552 57 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3087 4100 4117 4270 4455 4900 325 Size: 1255 1507 1013 17 153 185 445 1498 Fragments arranged by size: 1507 1498 1255 1013 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2859 3363 4319 Size: 274 80 33 51 1443 882 504 956 Cuts at: 4319 6031 96 Size: 1712 138 Fragments arranged by size: 1712 1443 956 882 504 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2918 2950 6033 6065 2918 Size: 32 3083 32 2926 Fragments arranged by size: 3083 2926 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6073 XbaI T'CTAG_A Cuts at: 3409 3409 Size: 6073 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6073 XmaI C'CCGG_G Cuts at: 1870 2890 3398 1870 Size: 1020 508 4545 Fragments arranged by size: 4545 1020 508 XmnI GAAnn'nnTTC Cuts at: 4202 4202 Size: 6073 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3881 276 Size: 53 83 186 936 2347 2468 Fragments arranged by size: 2468 2347 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI