(Circular) (Six-base) MAPSORT of: pFN21AE1409.seq Check: 334 from: 1 to: 6109 pFN21AE1409 6109 bp With 182 enzymes: SgfI * August 16, 2010 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3919 278 Size: 53 83 186 936 2383 2468 Fragments arranged by size: 2468 2383 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3452 1937 Size: 1515 4594 Acc65I G'GTAC_C Cuts at: 3430 3430 Size: 6109 AclI AA'CG_TT Cuts at: 1563 4236 4609 1563 Size: 2673 373 3063 Fragments arranged by size: 3063 2673 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3054 4172 5556 5824 1632 Size: 1422 1118 1384 268 1917 Fragments arranged by size: 1917 1422 1384 1118 268 AfeI AGC'GCT Cuts at: 2166 2166 Size: 6109 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5912 AflIII A'CryG_T Cuts at: 1203 5008 5985 1203 Size: 3805 977 1327 Fragments arranged by size: 3805 1327 977 AgeI A'CCGG_T Cuts at: 2442 4940 2442 Size: 2498 3611 AhdI GACnn_n'nnGTC Cuts at: 2920 4838 2920 Size: 1918 4191 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6109 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2587 1184 Size: 1403 4706 AlwNI CAG_nnn'CTG Cuts at: 3013 3032 3392 3852 5424 3013 Size: 19 360 460 1572 3698 Fragments arranged by size: 3698 1572 460 360 19 ApaI G_GGCC'C Cuts at: 2209 2209 Size: 6109 ApaLI G'TGCA_C Cuts at: 2446 4166 5322 2446 Size: 1720 1156 3233 Fragments arranged by size: 3233 1720 1156 ApoI r'AATT_y Cuts at: 1493 3418 3644 3796 5905 1493 Size: 1925 226 152 2109 1697 Fragments arranged by size: 2109 1925 1697 226 152 AscI GG'CGCG_CC Cuts at: 2218 2218 Size: 6109 AseI AT'TA_AT Cuts at: 160 1032 4663 160 Size: 872 3631 1606 Fragments arranged by size: 3631 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6109 AvaI C'yCGr_G Cuts at: 1870 1943 2063 2202 2208 3434 1870 Size: 73 120 139 6 1226 4545 Fragments arranged by size: 4545 1226 139 120 73 6 BamHI G'GATC_C Cuts at: 3439 3806 3439 Size: 367 5742 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 2634 3430 4885 618 Size: 325 217 627 221 626 796 1455 1842 Fragments arranged by size: 1842 1455 796 627 626 325 221 217 BanII G_rGCy'C Cuts at: 1391 2123 2209 3428 1391 Size: 732 86 1219 4072 Fragments arranged by size: 4072 1219 732 86 BbeI G_GCGC'C Cuts at: 2012 2012 Size: 6109 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5138 BbvCI CC'TCA_GC Cuts at: 2877 2877 Size: 6109 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4300 4334 1903 Size: 34 2363 34 3678 Fragments arranged by size: 3678 2363 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4001 5211 4001 Size: 1210 4899 BclI T'GATC_A Cuts at: 1548 2347 1548 Size: 799 5310 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4720 136 Size: 107 122 71 631 605 3048 1525 Fragments arranged by size: 3048 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6104 6104 Size: 6109 BlpI GC'TnA_GC Cuts at: 2323 3526 2323 Size: 1203 4906 Bme1580I G_kGCm'C Cuts at: 1790 2203 2209 2450 2639 3132 4170 5326 1790 Size: 413 6 241 189 493 1038 1156 2573 Fragments arranged by size: 2573 1156 1038 493 413 241 189 6 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6109 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2190 3840 4798 Size: 416 53 83 399 24 773 1650 958 Cuts at: 4798 442 Size: 1753 Fragments arranged by size: 1753 1650 958 773 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6109 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2183 2183 Size: 6109 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2451 2695 4769 1239 Size: 1212 244 2074 2579 Fragments arranged by size: 2579 2074 1212 244 Bpu10I CC'TnA_GC Cuts at: 2800 2877 2800 Size: 77 6032 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4234 5099 5397 4234 Size: 865 298 4946 Fragments arranged by size: 4946 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2861 3045 4772 882 Size: 617 1362 184 1727 2219 Fragments arranged by size: 2219 1727 1362 617 184 BsaAI yAC'GTr Cuts at: 493 1204 3900 493 Size: 711 2696 2702 Fragments arranged by size: 2702 2696 711 BsaBI GATnn'nnATC Cuts at: 6103 6103 Size: 6109 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 3916 4298 4933 Size: 53 83 186 936 476 1907 382 635 Cuts at: 4933 275 Size: 1451 Fragments arranged by size: 1907 1451 936 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2442 2589 4542 4940 5214 5361 5772 2442 Size: 147 1953 398 274 147 411 2779 Fragments arranged by size: 2779 1953 411 398 274 147 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2993 3023 1178 Size: 30 165 30 1590 30 4264 Fragments arranged by size: 4264 1590 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2175 3061 3064 1186 Size: 989 886 3 4231 Fragments arranged by size: 4231 989 886 3 BseYI C'CCAG_C Cuts at: 2105 3016 5312 5808 2105 Size: 911 2296 496 2406 Fragments arranged by size: 2406 2296 911 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2527 2644 1163 Size: 573 791 117 4628 Fragments arranged by size: 4628 791 573 117 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3559 4320 5348 1540 Size: 275 186 1558 761 1028 2301 Fragments arranged by size: 2301 1558 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2450 4170 4255 5326 2450 Size: 1720 85 1071 3233 Fragments arranged by size: 3233 1720 1071 85 BsmI GAATG_Cn' Cuts at: 3620 3713 3620 Size: 93 6016 BsmBI CGTCTCn'nnnn_ Cuts at: 2938 2938 Size: 6109 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2123 2203 2209 2450 2639 3132 3428 Size: 399 333 80 6 241 189 493 296 Cuts at: 3428 4170 4255 5326 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 493 399 333 296 241 189 85 80 6 BspEI T'CCGG_A Cuts at: 2589 5772 2589 Size: 3183 2926 BspHI T'CATG_A Cuts at: 2344 3153 3998 5728 2344 Size: 809 845 1730 2725 Fragments arranged by size: 2725 1730 845 809 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2299 3464 844 Size: 1455 1165 3489 Fragments arranged by size: 3489 1455 1165 BsrBI CCG'CTC Cuts at: 1394 1823 3996 1394 Size: 429 2173 3507 Fragments arranged by size: 3507 2173 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2375 2716 3240 4604 4778 65 Size: 1183 1127 341 524 1364 174 1396 Fragments arranged by size: 1396 1364 1183 1127 524 341 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2236 2442 2962 3022 4753 4940 1700 Size: 110 426 206 520 60 1731 187 2869 Fragments arranged by size: 2869 1731 520 426 206 187 110 60 BsrGI T'GTAC_A Cuts at: 96 2504 2986 96 Size: 2408 482 3219 Fragments arranged by size: 3219 2408 482 BssHII G'CGCG_C Cuts at: 1925 2218 1925 Size: 293 5816 BssSI C'ACGA_G Cuts at: 2145 4169 5181 2145 Size: 2024 1012 3073 Fragments arranged by size: 3073 2024 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2634 3074 794 Size: 1840 440 3829 Fragments arranged by size: 3829 1840 440 BstBI TT'CG_AA Cuts at: 4913 4913 Size: 6109 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6109 BstXI CCAn_nnnn'nTGG Cuts at: 2647 2827 2647 Size: 180 5929 BstYI r'GATC_y Cuts at: 1972 2408 3439 3806 4191 4208 5660 5746 5758 Size: 436 1031 367 385 17 1452 86 12 Cuts at: 5758 6104 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1031 436 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2094 2127 2178 513 Size: 552 939 90 33 51 4444 Fragments arranged by size: 4444 939 552 90 51 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5370 Fragments arranged by size: 5370 683 56 BtsI GCAGTG_nn' Cuts at: 2055 3625 4419 4439 2055 Size: 1570 794 20 3725 Fragments arranged by size: 3725 1570 794 20 ClaI AT'CG_AT Cuts at: 1554 3186 1554 Size: 1632 4477 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6074 DraI TTT'AAA Cuts at: 3413 3413 Size: 6109 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5116 809 Size: 507 3800 1802 Fragments arranged by size: 3800 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2097 3556 4445 Size: 54 1425 50 275 18 267 1459 889 Cuts at: 4445 6074 8 Size: 1629 43 Fragments arranged by size: 1629 1459 1425 889 275 267 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3556 1537 Size: 275 1744 4090 Fragments arranged by size: 4090 1744 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2128 2523 3306 4039 1353 Size: 775 395 783 733 3423 Fragments arranged by size: 3423 783 775 733 395 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4696 5070 5216 387 Size: 1064 138 3107 374 146 1280 Fragments arranged by size: 3107 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2451 2695 3054 4172 4769 5556 5824 Size: 393 819 244 359 1118 597 787 268 Cuts at: 5824 1239 Size: 1524 Fragments arranged by size: 1524 1118 819 787 597 393 359 268 244 EcoNI CCTnn'n_nnAGG Cuts at: 2306 3301 2306 Size: 995 5114 EcoO109I rG'GnC_Cy Cuts at: 2228 2299 2815 2867 3340 2228 Size: 71 516 52 473 4997 Fragments arranged by size: 4997 516 473 71 52 EcoRI G'AATT_C Cuts at: 3418 3796 3418 Size: 378 5731 FspI TGC'GCA Cuts at: 4615 4615 Size: 6109 HaeII r_GCGC'y Cuts at: 1678 2012 2168 2851 5256 1678 Size: 334 156 683 2405 2531 Fragments arranged by size: 2531 2405 683 334 156 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2044 2076 2634 2666 2906 Size: 32 135 32 472 32 558 32 240 Cuts at: 2906 2938 4750 4782 4824 4856 1373 Size: 32 1812 32 42 32 2626 Fragments arranged by size: 2626 1812 558 472 240 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3253 3453 3699 669 Size: 988 281 1315 200 246 3079 Fragments arranged by size: 3079 1315 988 281 246 200 HindIII A'AGCT_T Cuts at: 748 748 Size: 6109 HpaI GTT'AAC Cuts at: 3699 3699 Size: 6109 KasI G'GCGC_C Cuts at: 2008 2008 Size: 6109 KpnI G_GTAC'C Cuts at: 3434 3434 Size: 6109 MfeI C'AATT_G Cuts at: 2355 3708 2355 Size: 1353 4756 MluI A'CGCG_T Cuts at: 5985 5985 Size: 6109 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2502 5223 5407 1305 Size: 1197 2721 184 2007 Fragments arranged by size: 2721 2007 1197 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4287 Fragments arranged by size: 4287 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2034 2368 2569 4067 4426 4585 518 Size: 648 868 334 201 1498 359 159 2042 Fragments arranged by size: 2042 1498 868 648 359 334 201 159 MspA1I CmG'CkG Cuts at: 1606 2096 2129 2327 3163 3525 4202 5350 5595 Size: 490 33 198 836 362 677 1148 245 Cuts at: 5595 1606 Size: 2120 Fragments arranged by size: 2120 1148 836 677 490 362 245 198 33 NaeI GCC'GGC Cuts at: 2238 2964 2238 Size: 726 5383 NarI GG'CG_CC Cuts at: 2009 2009 Size: 6109 NcoI C'CATG_G Cuts at: 513 1065 2004 2178 513 Size: 552 939 174 4444 Fragments arranged by size: 4444 939 552 174 NdeI CA'TA_TG Cuts at: 387 387 Size: 6109 NgoMIV G'CCGG_C Cuts at: 2236 2962 2236 Size: 726 5383 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6109 NotI GC'GGCC_GC Cuts at: 3556 3556 Size: 6109 NspI r_CATG'y Cuts at: 1132 1229 2568 3323 3467 5012 1132 Size: 97 1339 755 144 1545 2229 Fragments arranged by size: 2229 1545 1339 755 144 97 PasI CC'CwG_GG Cuts at: 1348 2996 3215 1348 Size: 1648 219 4242 Fragments arranged by size: 4242 1648 219 PciI A'CATG_T Cuts at: 5008 5008 Size: 6109 PflMI CCAn_nnn'nTGG Cuts at: 1108 3071 1108 Size: 1963 4146 PfoI T'CCnGG_A Cuts at: 2428 2428 Size: 6109 PmeI GTTT'AAAC Cuts at: 3413 3413 Size: 6109 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4178 4210 5691 5723 4178 Size: 32 1481 32 4564 Fragments arranged by size: 4564 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2228 2299 2815 3340 2228 Size: 71 516 525 4997 Fragments arranged by size: 4997 525 516 71 PshAI GACnn'nnGTC Cuts at: 1613 2978 1613 Size: 1365 4744 PsiI TTA'TAA Cuts at: 3679 3679 Size: 6109 PspOMI G'GGCC_C Cuts at: 2205 2205 Size: 6109 PstI C_TGCA'G Cuts at: 830 2310 3461 830 Size: 1480 1151 3478 Fragments arranged by size: 3478 1480 1151 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6109 PvuII CAG'CTG Cuts at: 2327 3163 2327 Size: 836 5273 SacII CC_GC'GG Cuts at: 2097 2130 2097 Size: 33 6076 SalI G'TCGA_C Cuts at: 1936 3451 1936 Size: 1515 4594 SanDI GG'GwC_CC Cuts at: 2815 2815 Size: 6109 SapI GCTCTTCn'nnn_ Cuts at: 2128 2128 Size: 6109 SbfI CC_TGCA'GG Cuts at: 3461 3461 Size: 6109 ScaI AGT'ACT Cuts at: 4357 6069 4357 Size: 1712 4397 SfcI C'TryA_G Cuts at: 826 1045 2306 3457 4592 5273 5464 826 Size: 219 1261 1151 1135 681 191 1471 Fragments arranged by size: 1471 1261 1151 1135 681 219 191 SfoI GGC'GCC Cuts at: 2010 2010 Size: 6109 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6109 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6109 SmaI CCC'GGG Cuts at: 1872 2065 2204 2210 3436 1872 Size: 193 139 6 1226 4545 Fragments arranged by size: 4545 1226 193 139 6 SmlI C'TyrA_G Cuts at: 820 1017 1943 4213 5114 5376 820 Size: 197 926 2270 901 262 1553 Fragments arranged by size: 2270 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3900 493 Size: 3407 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6109 SphI G_CATG'C Cuts at: 1132 3467 1132 Size: 2335 3774 SrfI GCCC'GGGC Cuts at: 2204 2210 2204 Size: 6 6103 SspI AAT'ATT Cuts at: 5 52 4033 5 Size: 47 3981 2081 Fragments arranged by size: 3981 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6109 StyI C'CwwG_G Cuts at: 513 1065 2004 2178 3297 3402 3548 513 Size: 552 939 174 1119 105 146 3074 Fragments arranged by size: 3074 1119 939 552 174 146 105 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2119 2716 4136 4153 4306 4491 4936 Size: 1255 539 597 1420 17 153 185 445 Cuts at: 4936 325 Size: 1498 Fragments arranged by size: 1498 1420 1255 597 539 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2026 2504 2986 Size: 274 80 33 51 1443 49 478 482 Cuts at: 2986 4355 6067 96 Size: 1369 1712 138 Fragments arranged by size: 1712 1443 1369 482 478 274 138 80 51 49 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2807 2822 2839 2854 2993 3025 6069 6101 2807 Size: 15 17 15 139 32 3044 32 2815 Fragments arranged by size: 3044 2815 139 32 32 17 15 15 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6109 XbaI T'CTAG_A Cuts at: 3445 3445 Size: 6109 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6109 XmaI C'CCGG_G Cuts at: 1870 2063 2202 2208 3434 1870 Size: 193 139 6 1226 4545 Fragments arranged by size: 4545 1226 193 139 6 XmnI GAAnn'nnTTC Cuts at: 4238 4238 Size: 6109 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3917 276 Size: 53 83 186 936 2383 2468 Fragments arranged by size: 2468 2383 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SexAI SfiI SwaI XcmI